##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937915_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2482438 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.982057960762766 33.0 31.0 34.0 30.0 34.0 2 32.14943575630086 34.0 31.0 34.0 30.0 34.0 3 32.24683758466475 34.0 31.0 34.0 30.0 34.0 4 35.764492406255464 37.0 35.0 37.0 35.0 37.0 5 35.76938477416153 37.0 35.0 37.0 35.0 37.0 6 35.79985602862992 37.0 36.0 37.0 35.0 37.0 7 35.73100879055187 37.0 35.0 37.0 35.0 37.0 8 35.700463818230304 37.0 35.0 37.0 35.0 37.0 9 37.35600446013153 39.0 37.0 39.0 35.0 39.0 10-11 37.25653974036814 39.0 37.0 39.0 34.0 39.0 12-13 37.12297205408554 39.0 37.0 39.0 34.0 39.0 14-15 38.318684897669144 40.0 38.0 41.0 33.5 41.0 16-17 38.13132674411204 40.0 38.0 41.0 33.0 41.0 18-19 38.298431622461464 40.0 38.0 41.0 33.5 41.0 20-21 38.272265208637634 40.0 38.0 41.0 34.0 41.0 22-23 38.16302441390279 40.0 38.0 41.0 33.5 41.0 24-25 38.029610407188414 40.0 38.0 41.0 33.0 41.0 26-27 37.826813197348734 40.0 38.0 41.0 33.0 41.0 28-29 37.6160071268648 40.0 37.5 41.0 32.0 41.0 30-31 37.37885377197739 40.0 37.0 41.0 32.0 41.0 32-33 37.07069139289682 40.0 36.5 41.0 31.0 41.0 34-35 36.77494704802295 39.0 36.0 41.0 30.0 41.0 36-37 36.47583947715915 39.0 35.5 41.0 30.0 41.0 38-39 36.14366803924207 39.0 35.0 40.0 29.5 41.0 40-41 35.98772235197818 39.0 35.0 40.0 29.0 41.0 42-43 35.726122666507685 39.0 35.0 40.0 27.5 41.0 44-45 35.381072961338816 38.5 35.0 40.0 26.5 41.0 46-47 34.94308740037012 38.0 34.0 40.0 24.5 41.0 48-49 34.47957793910663 38.0 33.5 40.0 23.5 41.0 50-51 33.64951914206921 37.5 32.5 39.5 22.5 40.5 52-53 33.62454570869444 38.0 33.0 39.5 22.0 40.5 54-55 33.59947096362528 38.0 33.0 40.0 19.5 41.0 56-57 33.11584901616878 37.5 32.5 40.0 15.5 41.0 58-59 32.63985324104771 37.0 31.5 40.0 10.5 41.0 60-61 32.06184283353703 36.0 31.0 39.5 7.5 41.0 62-63 31.424392875068783 36.0 30.5 39.0 2.0 41.0 64-65 30.801068546324217 35.0 29.5 39.0 2.0 40.0 66-67 30.10333208724649 35.0 28.5 38.0 2.0 40.0 68-69 29.376910722443018 34.5 27.5 38.0 2.0 40.0 70-71 28.531838861635215 34.0 26.0 37.0 2.0 39.5 72-73 27.71038813456771 34.0 25.5 36.0 2.0 39.0 74-75 26.87086525423797 33.0 22.5 36.0 2.0 39.0 76-77 26.029509901153624 32.5 19.5 35.0 2.0 37.5 78-79 25.18401265207832 32.0 15.5 35.0 2.0 37.0 80-81 24.37910493635692 31.0 7.5 35.0 2.0 36.5 82-83 23.534742458824752 31.0 2.0 35.0 2.0 36.0 84-85 22.71997085123576 30.0 2.0 34.0 2.0 35.5 86-87 21.942125442810656 29.5 2.0 34.0 2.0 35.0 88-89 21.098816566617174 29.0 2.0 34.0 2.0 35.0 90-91 20.22995800902178 28.0 2.0 34.0 2.0 35.0 92-93 19.317827071612665 26.0 2.0 34.0 2.0 35.0 94-95 18.370497269216795 24.5 2.0 33.5 2.0 35.0 96-97 17.25469397423017 20.5 2.0 33.0 2.0 35.0 98-99 15.933196116076212 2.0 2.0 32.0 2.0 34.0 100-101 12.87713308449194 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45779206833988795 1101 2 0.4355941859331338 1101 3 0.42132246050378797 1101 4 0.16118787833755022 1101 5 0.28453364465806885 1101 6 0.19757642793620533 1101 7 0.3135184034632488 1101 8 0.2001215317781444 1101 9 0.3458110945052013 1101 10-11 0.3862165308343606 1101 12-13 0.46742340548091477 1101 14-15 0.6206573852553916 1101 16-17 0.6796610990672107 1101 18-19 0.6941411664648527 1101 20-21 0.6498227819110411 1101 22-23 0.746869383644146 1101 24-25 0.8330732462625647 1101 26-27 0.8663192966956714 1101 28-29 0.8187881981295249 1101 30-31 0.9167685895825244 1101 32-33 0.9424871743824781 1101 34-35 1.0696422685336913 1101 36-37 1.0760558107579214 1101 38-39 1.189044825792763 1101 40-41 1.3669671705589863 1101 42-43 1.394497510483582 1101 44-45 1.62208339519497 1101 46-47 1.6570202444402753 1101 48-49 1.768313223626997 1101 50-51 1.973824040725745 1101 52-53 2.0642786377665345 1101 54-55 2.1986017881700093 1101 56-57 2.3571455201407048 1101 58-59 2.61479710880203 1101 60-61 2.7119240736480705 1101 62-63 2.829188314981703 1101 64-65 2.8872615321794655 1101 66-67 2.9981943131426654 1101 68-69 3.3533495538078704 1101 70-71 3.599774829525245 1101 72-73 3.9522337421748617 1101 74-75 4.150831996258319 1101 76-77 4.340863332214525 1101 78-79 4.4944539290672125 1101 80-81 4.613305091493544 1101 82-83 5.064381486137368 1101 84-85 4.961394524237011 1101 86-87 5.20542748374768 1101 88-89 5.221159846917665 1101 90-91 5.214214230338353 1101 92-93 5.6216232510232444 1101 94-95 5.441588921936901 1101 96-97 5.461791934204566 1101 98-99 5.611621145456413 1101 100-101 4.893628355095576 1102 1 0.3965241182159964 1102 2 0.3833113140354101 1102 3 0.37533496234487984 1102 4 0.22389893562150576 1102 5 0.18442409519597192 1102 6 0.2694648759816758 1102 7 0.29669658942184185 1102 8 0.3438383302017911 1102 9 0.35780048373737827 1102 10-11 0.307912036749876 1102 12-13 0.4976949820697669 1102 14-15 0.6511238499939012 1102 16-17 0.60305279009728 1102 18-19 0.5149393665110935 1102 20-21 0.512978676404714 1102 22-23 0.5243637802521164 1102 24-25 0.49138681414087415 1102 26-27 0.5205819002258281 1102 28-29 0.5689147848033258 1102 30-31 0.5724687674716193 1102 32-33 0.6477658291410293 1102 34-35 0.9156898834385245 1102 36-37 0.9562476768534438 1102 38-39 1.0855478665439406 1102 40-41 1.0060105645089266 1102 42-43 1.258455279566597 1102 44-45 1.323416685792182 1102 46-47 1.50208903051319 1102 48-49 1.50655897844225 1102 50-51 1.6900383722832402 1102 52-53 1.7944774103241485 1102 54-55 1.8714304689899564 1102 56-57 2.102720519358485 1102 58-59 2.2450535560416185 1102 60-61 2.3992851335648666 1102 62-63 2.5271160710988845 1102 64-65 2.446248537346719 1102 66-67 2.722901208233889 1102 68-69 2.7836259572887236 1102 70-71 3.21316300627268 1102 72-73 3.4255861503170095 1102 74-75 3.586769027639601 1102 76-77 3.7414406132357954 1102 78-79 3.9720848141551315 1102 80-81 4.057621263985677 1102 82-83 4.404551453787651 1102 84-85 4.547658906658118 1102 86-87 4.827302500441476 1102 88-89 4.925186180761539 1102 90-91 4.997122854410847 1102 92-93 4.983293588417272 1102 94-95 4.959000551655905 1102 96-97 4.87365632028877 1102 98-99 4.873850448392156 1102 100-101 4.160026909851609 1103 1 0.4729220799692406 1103 2 0.3802909760064068 1103 3 0.3752194221946752 1103 4 0.2090957241978444 1103 5 0.3615301106901967 1103 6 0.288537147826915 1103 7 0.2898419478185019 1103 8 0.29110313933835386 1103 9 0.419700378566219 1103 10-11 0.3767380451339477 1103 12-13 0.45363048696540886 1103 14-15 0.6218914513507556 1103 16-17 0.6969569362111301 1103 18-19 0.6342342012738627 1103 20-21 0.6265183525960367 1103 22-23 0.6312773028935439 1103 24-25 0.6450881724782747 1103 26-27 0.7454152557784255 1103 28-29 0.6695754374570555 1103 30-31 0.8356651561012782 1103 32-33 0.9179842444634332 1103 34-35 0.9064322289035758 1103 36-37 0.8883659870046898 1103 38-39 1.1183172010030518 1103 40-41 1.2809246500382656 1103 42-43 1.2804816019828635 1103 44-45 1.3865986319030768 1103 46-47 1.6163745331868995 1103 48-49 1.677733562571781 1103 50-51 1.883959923483829 1103 52-53 1.8591806350793618 1103 54-55 2.048507229125647 1103 56-57 2.2197097356111257 1103 58-59 2.478057674011076 1103 60-61 2.6193406076498107 1103 62-63 2.690731292773034 1103 64-65 2.7987630168975954 1103 66-67 3.0838667831942708 1103 68-69 3.1648866040173083 1103 70-71 3.275066974632452 1103 72-73 3.6554425398354446 1103 74-75 3.9354629054929227 1103 76-77 4.176128210445057 1103 78-79 4.341978457965581 1103 80-81 4.648818363631651 1103 82-83 4.8472383547934434 1103 84-85 4.769627088678295 1103 86-87 4.993667725818835 1103 88-89 5.276953352546045 1103 90-91 5.465614833258112 1103 92-93 5.435399233590509 1103 94-95 5.371761442796345 1103 96-97 5.19979987151898 1103 98-99 5.020336102156838 1103 100-101 4.4219201389025535 1104 1 0.5440592819485452 1104 2 0.39563330619084525 1104 3 0.46946503622976365 1104 4 0.3627430476346376 1104 5 0.31705147821156743 1104 6 0.36463791255926026 1104 7 0.2962009221774693 1104 8 0.4074459193653439 1104 9 0.4418092404645506 1104 10-11 0.48596521562427597 1104 12-13 0.47849193545843605 1104 14-15 0.7002810150034762 1104 16-17 0.8454711062516154 1104 18-19 0.7338149480125082 1104 20-21 0.7262385505267659 1104 22-23 0.6905795970905686 1104 24-25 0.8396999583532221 1104 26-27 0.79138286711445 1104 28-29 0.9124881680013601 1104 30-31 0.9326323175947593 1104 32-33 1.0418492916968987 1104 34-35 1.0856252113597122 1104 36-37 1.14886621192597 1104 38-39 1.276127817287346 1104 40-41 1.4527807004206323 1104 42-43 1.4591235273090035 1104 44-45 1.3981622846246182 1104 46-47 1.609663781949834 1104 48-49 1.8009175481768196 1104 50-51 1.9843544354046543 1104 52-53 1.9765980889705048 1104 54-55 2.1603988170698614 1104 56-57 2.418879326254398 1104 58-59 2.6133941562423004 1104 60-61 2.7436640444469234 1104 62-63 2.949558103389318 1104 64-65 3.0857909071360083 1104 66-67 3.1558312456324096 1104 68-69 3.356133117737503 1104 70-71 3.5762788504759087 1104 72-73 3.8117754233262247 1104 74-75 3.961265424611234 1104 76-77 4.26079352758406 1104 78-79 4.329175938847275 1104 80-81 4.522205228229044 1104 82-83 4.838386352994071 1104 84-85 4.890148006357105 1104 86-87 4.982325395634401 1104 88-89 5.048594828029621 1104 90-91 5.035763281301296 1104 92-93 5.115255733815431 1104 94-95 4.957160453142723 1104 96-97 4.718015346102874 1104 98-99 4.49700861677672 1104 100-101 3.7334824420964345 1105 1 0.3830969987395072 1105 2 0.29584537986215764 1105 3 0.41808045390830273 1105 4 0.27477210353285386 1105 5 0.2617178726582807 1105 6 0.289290638698823 1105 7 0.37894163267155534 1105 8 0.32376063428657886 1105 9 0.3549326205767329 1105 10-11 0.3872167683525589 1105 12-13 0.5103067685804419 1105 14-15 0.6342299952532002 1105 16-17 0.6789158205281183 1105 18-19 0.6386204325282065 1105 20-21 0.6183058604921712 1105 22-23 0.6885704419037069 1105 24-25 0.6528012456670567 1105 26-27 0.7169122561239334 1105 28-29 0.9188787463254897 1105 30-31 0.9579643328104268 1105 32-33 0.9796661449775073 1105 34-35 1.0532336444473316 1105 36-37 1.1129648988228737 1105 38-39 1.097203315342412 1105 40-41 1.1914389712508253 1105 42-43 1.381641590535672 1105 44-45 1.4969473526437937 1105 46-47 1.5934701905806463 1105 48-49 1.5768790362170435 1105 50-51 1.7349735065615164 1105 52-53 1.6307777111578616 1105 54-55 1.742924015324789 1105 56-57 2.2292124804956615 1105 58-59 2.298926038213459 1105 60-61 2.511124189296595 1105 62-63 2.537033463901661 1105 64-65 2.689340454749427 1105 66-67 2.9335290385016783 1105 68-69 2.865504246662315 1105 70-71 3.2004677138143265 1105 72-73 3.367262818825999 1105 74-75 3.4507767611395685 1105 76-77 3.707706510326748 1105 78-79 3.9759602331886796 1105 80-81 3.932047956732621 1105 82-83 4.079075699881091 1105 84-85 4.050200530480808 1105 86-87 4.2730908638169005 1105 88-89 4.785040466421236 1105 90-91 4.734257188588913 1105 92-93 4.709147719803266 1105 94-95 4.516253874732634 1105 96-97 4.469017989501896 1105 98-99 4.220337236406644 1105 100-101 3.686339333201289 1106 1 0.412993519207685 1106 2 0.33836843739553046 1106 3 0.2916626024736928 1106 4 0.3058670665400882 1106 5 0.33591860539564067 1106 6 0.2945214430025942 1106 7 0.2272240834940078 1106 8 0.2208414154195779 1106 9 0.2599921785085684 1106 10-11 0.23895616923748264 1106 12-13 0.3371280055198085 1106 14-15 0.5100635167678433 1106 16-17 0.46362855187706487 1106 18-19 0.5066149651215142 1106 20-21 0.5592819432426097 1106 22-23 0.46271048098458323 1106 24-25 0.48752228132848074 1106 26-27 0.5910986865288947 1106 28-29 0.6738077000700571 1106 30-31 0.6604801329885888 1106 32-33 0.7813001781572595 1106 34-35 0.876694994716928 1106 36-37 0.9145661627375787 1106 38-39 0.9638947926289916 1106 40-41 1.0182164881834126 1106 42-43 0.9497185357565741 1106 44-45 1.0766851786129408 1106 46-47 1.2809707816718117 1106 48-49 1.344930379931128 1106 50-51 1.5288588944392814 1106 52-53 1.4996791642715195 1106 54-55 1.709549141071662 1106 56-57 1.6261863259763771 1106 58-59 2.0131690872716206 1106 60-61 2.201217285534206 1106 62-63 1.9934346619128114 1106 64-65 2.3434096757153213 1106 66-67 2.4489830238996966 1106 68-69 2.291605903725234 1106 70-71 2.6892570578798285 1106 72-73 3.155595351040674 1106 74-75 3.1329377871510644 1106 76-77 3.0384280364486607 1106 78-79 3.4856758639953753 1106 80-81 3.66908364735691 1106 82-83 3.7841705656590143 1106 84-85 3.9696932489841927 1106 86-87 4.0321363838933415 1106 88-89 4.184870796292419 1106 90-91 4.281826712940127 1106 92-93 4.537068261884894 1106 94-95 4.5521937943176844 1106 96-97 4.34035448936941 1106 98-99 4.290078528451476 1106 100-101 3.6631254199188845 1107 1 0.47446512463549695 1107 2 0.4545336228851582 1107 3 0.4021398751896257 1107 4 0.3235799823783765 1107 5 0.33220334067574697 1107 6 0.36565719428793386 1107 7 0.35916612889052857 1107 8 0.34837876724434835 1107 9 0.3771693025107865 1107 10-11 0.40990799520933763 1107 12-13 0.4467973408056096 1107 14-15 0.4757309579123259 1107 16-17 0.5851421750410708 1107 18-19 0.4698454540770953 1107 20-21 0.4729431630609682 1107 22-23 0.6229423952033315 1107 24-25 0.6413914076067257 1107 26-27 0.6793820053612194 1107 28-29 0.812988415755413 1107 30-31 0.8525833700108976 1107 32-33 0.9018271446387089 1107 34-35 1.0757390920499788 1107 36-37 1.1682778929910924 1107 38-39 1.317848479975595 1107 40-41 1.3243289693738163 1107 42-43 1.306623710431559 1107 44-45 1.5020159589249786 1107 46-47 1.7763521073478614 1107 48-49 1.7610431056234361 1107 50-51 1.815765168541219 1107 52-53 1.9623645969309464 1107 54-55 1.8917442293110724 1107 56-57 2.265243460084676 1107 58-59 2.487883847519207 1107 60-61 2.551480491007453 1107 62-63 2.7113676128362947 1107 64-65 2.788877017828213 1107 66-67 2.7931212902281395 1107 68-69 2.8721280333934125 1107 70-71 3.172651386400247 1107 72-73 3.3442201741718165 1107 74-75 3.542917922618429 1107 76-77 3.804328353186744 1107 78-79 3.9231746893050783 1107 80-81 3.9654403360559165 1107 82-83 4.318281908501753 1107 84-85 4.248650882458531 1107 86-87 4.433751726504553 1107 88-89 4.6395262540157916 1107 90-91 4.471246832586385 1107 92-93 4.333889337882905 1107 94-95 4.381798667590019 1107 96-97 4.474982147059418 1107 98-99 4.432446667490895 1107 100-101 3.611253258823062 1108 1 0.3973445037366439 1108 2 0.36916306946365296 1108 3 0.29428783558623195 1108 4 0.23996759371600263 1108 5 0.13603837777488792 1108 6 0.13860619571255484 1108 7 0.20699568200279828 1108 8 0.13457531490659846 1108 9 0.4167630441878316 1108 10-11 0.3827489435029179 1108 12-13 0.37053882123181836 1108 14-15 0.41254945903933304 1108 16-17 0.43880275414865366 1108 18-19 0.4633332196741904 1108 20-21 0.46504893894322663 1108 22-23 0.4735150142360567 1108 24-25 0.4557849962755398 1108 26-27 0.5356067236991464 1108 28-29 0.580762128010555 1108 30-31 0.533701956758172 1108 32-33 0.6167805718623782 1108 34-35 0.7071600309414166 1108 36-37 0.7877184298069082 1108 38-39 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0.48201200646017384 2311 34-35 0.6769393682438007 2311 36-37 0.7146341509759537 2311 38-39 0.8428421918500533 2311 40-41 0.890976222460587 2311 42-43 0.9741199511603611 2311 44-45 1.0557179870268598 2311 46-47 1.1846410811801888 2311 48-49 1.4292349702400955 2311 50-51 1.44066812583457 2311 52-53 1.5031440735775163 2311 54-55 1.716055740027187 2311 56-57 1.8808536712750055 2311 58-59 1.909979867196192 2311 60-61 2.0096516834113984 2311 62-63 2.1787945179115944 2311 64-65 2.3777657186107746 2311 66-67 2.4847154872556736 2311 68-69 2.6073423224331442 2311 70-71 3.1459329747082023 2311 72-73 3.2740165023902037 2311 74-75 3.5133000199622373 2311 76-77 3.466463589000522 2311 78-79 3.7393998368492944 2311 80-81 3.9234872775176974 2311 82-83 4.349563216867196 2311 84-85 4.579072984977955 2311 86-87 4.536011083265588 2311 88-89 4.739694960191638 2311 90-91 5.06345977512078 2311 92-93 5.237942820809135 2311 94-95 5.480605450421745 2311 96-97 5.542581310496871 2311 98-99 5.536407823804629 2311 100-101 5.27371854564926 2312 1 -0.25523456009910817 2312 2 -0.21572036803485162 2312 3 -0.10050719826014642 2312 4 -0.03335104019346602 2312 5 -0.0890805903765397 2312 6 -0.10050266233657368 2312 7 -0.09260316392249024 2312 8 -0.091750301760257 2312 9 -0.05274526327016105 2312 10-11 -0.15108572621409166 2312 12-13 -0.17053773100980862 2312 14-15 -0.12302165977906299 2312 16-17 0.0638113969637999 2312 18-19 -0.10985071377054823 2312 20-21 -0.05600081658857903 2312 22-23 -0.09112304696940754 2312 24-25 0.05455707926568465 2312 26-27 0.101165543433126 2312 28-29 0.13719947198185167 2312 30-31 0.15443411870460722 2312 32-33 0.21169325951502316 2312 34-35 0.35170159531485723 2312 36-37 0.3476811661301582 2312 38-39 0.41925414459778665 2312 40-41 0.4211071275793685 2312 42-43 0.5926628041768325 2312 44-45 0.7377523689196366 2312 46-47 0.705235202292009 2312 48-49 0.8940659573244574 2312 50-51 0.9456498519833119 2312 52-53 1.0954807096045371 2312 54-55 1.370566447016671 2312 56-57 1.5159742781544026 2312 58-59 1.643800961288484 2312 60-61 1.7160731152816169 2312 62-63 1.969156098742527 2312 64-65 2.163011591096918 2312 66-67 2.4940732241650245 2312 68-69 2.6171084052814315 2312 70-71 2.950677130012764 2312 72-73 3.1553334025500916 2312 74-75 2.7731986030516786 2312 76-77 2.899864400680702 2312 78-79 3.5176046721178835 2312 80-81 3.907929378078311 2312 82-83 4.140416806727824 2312 84-85 4.381360126405493 2312 86-87 4.5694309852194515 2312 88-89 4.803612551104184 2312 90-91 4.925125212213949 2312 92-93 4.968227027427059 2312 94-95 5.185926059241151 2312 96-97 4.96338941242519 2312 98-99 4.764266743852891 2312 100-101 4.36511238890801 2313 1 -0.43505915771496717 2313 2 -0.3303903668531518 2313 3 -0.3146909974187153 2313 4 -0.1202107229303877 2313 5 -0.23311259533864614 2313 6 -0.19465353387862194 2313 7 -0.16472411318320468 2313 8 -0.13132278457272406 2313 9 -0.16067776913866538 2313 10-11 -0.18228104358811947 2313 12-13 -0.17780092540660064 2313 14-15 -0.1827912696685985 2313 16-17 -0.061964835086882886 2313 18-19 -0.15857156281902718 2313 20-21 -0.09024049361824638 2313 22-23 -0.09400195580155923 2313 24-25 0.04950886179995706 2313 26-27 -0.05034690285502563 2313 28-29 0.024049621139155875 2313 30-31 0.04485832816573776 2313 32-33 0.13669095029884915 2313 34-35 0.2257509464896188 2313 36-37 0.30010331458360184 2313 38-39 0.2386344799836948 2313 40-41 0.5403928496054746 2313 42-43 0.5795924471333151 2313 44-45 0.7154759152357997 2313 46-47 0.6520912367253899 2313 48-49 0.8516044386703712 2313 50-51 0.8886299509261306 2313 52-53 0.9536323329964134 2313 54-55 1.1855990701778865 2313 56-57 1.422165338314258 2313 58-59 1.5267390531380087 2313 60-61 1.8041593917260101 2313 62-63 1.740683540745085 2313 64-65 1.9044745221140431 2313 66-67 2.1037678941844824 2313 68-69 2.1468901340406426 2313 70-71 2.447789384236053 2313 72-73 2.9488938629897383 2313 74-75 2.891475429822279 2313 76-77 3.005539228469935 2313 78-79 3.1168711670167113 2313 80-81 3.342149097803972 2313 82-83 3.6318921223680043 2313 84-85 3.8940486242384402 2313 86-87 3.9937762256798095 2313 88-89 4.206373135183647 2313 90-91 4.336331657687811 2313 92-93 4.468907859178341 2313 94-95 4.623731062835272 2313 96-97 4.6422148547755455 2313 98-99 4.4311761147295226 2313 100-101 4.269553089010749 2314 1 -0.27362208766867724 2314 2 -0.22497988160819915 2314 3 -0.09724743386593104 2314 4 -0.17534726337014916 2314 5 -0.18465590815206667 2314 6 -0.05036388169911277 2314 7 -0.004975214186160315 2314 8 -0.07030602441456324 2314 9 -0.04119584326750214 2314 10-11 -0.09349007743792725 2314 12-13 -0.013051051981754824 2314 14-15 0.031437937850363085 2314 16-17 0.06904773603631043 2314 18-19 -0.07306050055208146 2314 20-21 -0.008473695286625116 2314 22-23 0.030092680224548474 2314 24-25 0.06691369938192793 2314 26-27 0.05953238864555033 2314 28-29 0.09076904419752907 2314 30-31 0.1786123564703601 2314 32-33 0.244845404137088 2314 34-35 0.2909256001156919 2314 36-37 0.3054317771173558 2314 38-39 0.37686936932084336 2314 40-41 0.4757578983871369 2314 42-43 0.5578730039836444 2314 44-45 0.6421050178782082 2314 46-47 0.7340388567106046 2314 48-49 0.8551990224591108 2314 50-51 0.8355731106438924 2314 52-53 0.9838197546403578 2314 54-55 1.1885989516392428 2314 56-57 1.5348651964697098 2314 58-59 1.771938489376275 2314 60-61 1.8150604260258731 2314 62-63 1.9469123946572573 2314 64-65 2.2204962749589754 2314 66-67 2.3340628544840847 2314 68-69 2.6430692720437285 2314 70-71 2.8867301812175334 2314 72-73 3.066576111125265 2314 74-75 3.2746395274820443 2314 76-77 3.6121330909811853 2314 78-79 3.9601881996128228 2314 80-81 4.173674800597464 2314 82-83 4.301421714836177 2314 84-85 4.4546859100652405 2314 86-87 4.627525231160714 2314 88-89 4.9008239697760345 2314 90-91 4.98358176216254 2314 92-93 5.139577130346531 2314 94-95 4.93901571630062 2314 96-97 4.78930975399761 2314 98-99 4.8082701299346144 2314 100-101 4.49793661374715 2315 1 -0.5706516665456824 2315 2 -0.4516551824103381 2315 3 -0.37362193252903353 2315 4 -0.30066477673913283 2315 5 -0.26649219380269074 2315 6 -0.1529318407009015 2315 7 -0.293731341637141 2315 8 -0.3904750863575188 2315 9 -0.5001725589537784 2315 10-11 -0.4102723301117237 2315 12-13 -0.350913824931844 2315 14-15 -0.37712587119597885 2315 16-17 -0.2547330404788397 2315 18-19 -0.4341692906590353 2315 20-21 -0.42417180691764855 2315 22-23 -0.26447547374159086 2315 24-25 -0.22769623265906347 2315 26-27 -0.2913254544916981 2315 28-29 -0.2024719595903477 2315 30-31 -0.35197824624800234 2315 32-33 -0.26440983204829394 2315 34-35 -0.0489153646793099 2315 36-37 0.01836278758973009 2315 38-39 0.07790059146879003 2315 40-41 0.11808814014293745 2315 42-43 0.21483532016011253 2315 44-45 0.3098286698389856 2315 46-47 0.4262173047943776 2315 48-49 0.6463998635910215 2315 50-51 0.7307456540930843 2315 52-53 0.8978147134193293 2315 54-55 0.9918764416125043 2315 56-57 1.125978303644402 2315 58-59 1.3799647538593334 2315 60-61 1.4980999202326473 2315 62-63 1.506920332491486 2315 64-65 1.820193383916191 2315 66-67 1.9689266579600222 2315 68-69 2.323866676232676 2315 70-71 2.6130691685170007 2315 72-73 2.8123829850111832 2315 74-75 3.0986730613501408 2315 76-77 3.2830012393769117 2315 78-79 3.595925164853469 2315 80-81 3.833377758485163 2315 82-83 4.017842587162381 2315 84-85 4.35139727927201 2315 86-87 4.713492672114366 2315 88-89 4.838033612155712 2315 90-91 4.958333878918335 2315 92-93 5.115577715239578 2315 94-95 5.226816518439659 2315 96-97 5.23276870007604 2315 98-99 5.111705680291118 2315 100-101 4.82590912377389 2316 1 -0.6165645498710859 2316 2 -0.617003387365127 2316 3 -0.5889463512958564 2316 4 -0.453296064078188 2316 5 -0.3954572749145484 2316 6 -0.43520759617506855 2316 7 -0.3728122968293164 2316 8 -0.4326842022946522 2316 9 -0.4518718862536346 2316 10-11 -0.44630315089760586 2316 12-13 -0.3890777604856055 2316 14-15 -0.39544715859235424 2316 16-17 -0.42111064286930855 2316 18-19 -0.5285794409610034 2316 20-21 -0.4964747493359667 2316 22-23 -0.43062323850860906 2316 24-25 -0.4576559394298201 2316 26-27 -0.37411781922088494 2316 28-29 -0.24853061690302525 2316 30-31 -0.42675686275160274 2316 32-33 -0.18228233425973883 2316 34-35 -0.05843022197174008 2316 36-37 0.1278883438903904 2316 38-39 0.038572374554043165 2316 40-41 0.014912063125358088 2316 42-43 0.20616453819634728 2316 44-45 0.40459819218558835 2316 46-47 0.4700819012029598 2316 48-49 0.6574144925742544 2316 50-51 0.576886655558468 2316 52-53 0.5807975587059317 2316 54-55 0.9032953402642363 2316 56-57 0.9721391746965153 2316 58-59 1.154030548938941 2316 60-61 1.3339385409776412 2316 62-63 1.615965579743655 2316 64-65 1.9854141560406902 2316 66-67 2.113869943663662 2316 68-69 2.3352750669298885 2316 70-71 2.764201830487565 2316 72-73 3.084936589908274 2316 74-75 3.2471147316945874 2316 76-77 3.5918611645220615 2316 78-79 3.833425952950055 2316 80-81 4.026477280277707 2316 82-83 4.108772693980516 2316 84-85 4.29767144517924 2316 86-87 4.473277201730561 2316 88-89 4.653798791851287 2316 90-91 4.882346083429876 2316 92-93 5.038472446551182 2316 94-95 5.440388974607156 2316 96-97 5.460225985240914 2316 98-99 5.427544033560908 2316 100-101 5.348203564301734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6175.0 3 2079.0 4 2239.0 5 2555.0 6 3022.0 7 3719.0 8 4742.0 9 6214.0 10 7984.0 11 9993.0 12 12791.0 13 16119.0 14 18946.0 15 23103.0 16 27173.0 17 32872.0 18 34222.0 19 33093.0 20 35353.0 21 39324.0 22 43757.0 23 49103.0 24 54702.0 25 60554.0 26 65524.0 27 72374.0 28 79854.0 29 87492.0 30 96734.0 31 108654.0 32 124148.0 33 144891.0 34 175763.0 35 220118.0 36 274202.0 37 308440.0 38 180443.0 39 13967.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34464504066198 17.854029320618242 13.55666151248326 24.24466412623652 2 15.828398492571852 19.117607358724946 40.01445699828716 25.03953715041604 3 19.905203720115214 22.434743429004147 28.96916602084132 28.690886830039318 4 12.900267136920062 15.521868032185385 36.087985528102166 35.48987930279238 5 14.07394001880685 36.66646379238141 34.27679280744825 14.982803381363485 6 34.086480076838214 34.90274508002033 16.592363657908706 14.418411185232745 7 29.984123466242217 31.660669312755623 19.763786190639294 18.591421030362866 8 26.812330609656147 34.99561186834477 19.018541367307975 19.17351615469111 9 26.238588081314756 16.517906074106552 19.741329843134473 37.50217600144422 10-11 25.628389053807005 25.906444082954327 26.71953493226224 21.74563193097643 12-13 26.270283905163737 23.053387806547825 27.27936675895522 23.39696152933321 14-15 22.76464579921067 24.84984013973419 25.343019636521873 27.042494424533263 16-17 22.77336797598761 27.911802262866985 26.34828923016667 22.96654053097873 18-19 22.738228780860467 26.577816405406363 28.232733611194384 22.451221202538786 20-21 22.977290330276308 25.81573710718284 28.701776104594884 22.505196457945967 22-23 22.736172902109562 25.752345908980928 28.50762172365004 23.003859465259474 24-25 22.68502089949121 25.891973922335666 28.650126416582083 22.77287876159104 26-27 22.39015434065201 26.19922392431227 28.47191914111819 22.93870259391753 28-29 22.373602710100702 26.74961185552201 28.35209549663756 22.52468993773973 30-31 22.578392354277184 26.155816742508136 28.300252428780748 22.96553847443393 32-33 22.32459298738655 26.145319249164938 28.429127358348005 23.10096040510051 34-35 23.020423985892926 26.313410907191837 28.13456039510121 22.53160471181403 36-37 23.020586472897723 25.964072903524006 28.15368509027108 22.861655533307193 38-39 22.541123629548096 26.24343883308327 28.264568328802092 22.950869208566544 40-41 23.00764502215429 26.130436744451757 28.17351412371109 22.68840410968286 42-43 22.698129052830858 26.143585460682388 27.96090951411454 23.19737597237221 44-45 22.766893308747427 26.381295320526586 28.16619738850632 22.685613982219667 46-47 23.096727145264467 26.656446445065136 27.558538803770738 22.68828760589966 48-49 22.980273407676865 26.526390556956038 27.61011828381104 22.883217751556053 50-51 22.93419273381166 26.797627342407154 27.610959733026668 22.657220190754515 52-53 23.171810550800394 26.60559642884335 27.52801497623405 22.694578044122203 54-55 22.753532868904266 26.645983150514578 27.66699928414059 22.933484696440562 56-57 22.928558623342983 26.788773872039222 27.48763719939007 22.79503030522773 58-59 22.959068370046396 26.48353844065262 27.45122683705298 23.106166352248003 60-61 22.69168435525644 26.707763011436587 27.710161193443007 22.890391439863965 62-63 22.703651698123924 26.66840834256039 27.897941552606703 22.729998406708983 64-65 22.468437361075942 26.705123765911242 27.74208766131808 23.084351211694727 66-67 22.52292101490173 26.770363638341248 27.521110215446864 23.18560513131015 68-69 22.87734428614275 26.348782200919274 27.76618563819512 23.007687874742853 70-71 22.812400094358264 26.471462164329633 27.41363468887054 23.302503052441562 72-73 22.83669593482309 26.54465603000033 27.434666367995565 23.183981667181015 74-75 22.728246022523205 26.905012115282616 27.24019925102596 23.12654261116822 76-77 22.9935326937669 26.533862663961333 27.29327311119791 23.179331531073867 78-79 23.014588831900408 26.625368537859718 27.274128113499085 23.08591451674079 80-81 23.222363226170334 26.676214648295865 27.021029731689627 23.08039239384417 82-83 23.145267776118157 26.737163133282237 27.022358573136472 23.095210517463137 84-85 23.13257055104991 26.69718520929648 26.974929863885176 23.195314375768437 86-87 23.23116580312968 26.676203036867967 26.899855222270475 23.19277593773188 88-89 23.17434818223539 26.78556103127202 26.72588821238205 23.31420257411054 90-91 23.25593014070933 26.69017093543592 26.700001873518936 23.353897050335814 92-93 23.419373202270876 26.316306857291 26.84591365179305 23.41840628864507 94-95 23.221550253631822 26.15603993076926 27.031920399557507 23.59048941604141 96-97 23.49012027707745 25.879364337509102 26.90237925202787 23.728136133385576 98-99 23.27646155383366 26.116894492219227 26.517939449221924 24.088704504725193 100-101 24.094307038246452 25.027961234347103 26.70384077571632 24.173890951690126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 452.0 1 318.0 2 277.0 3 398.5 4 336.0 5 314.0 6 610.5 7 1223.5 8 1979.0 9 2344.0 10 2094.0 11 1785.5 12 1783.5 13 1917.5 14 2072.5 15 2280.0 16 2446.5 17 2584.0 18 2852.5 19 3307.5 20 3888.5 21 4706.0 22 5776.5 23 7091.0 24 8951.0 25 11290.5 26 14195.5 27 17615.0 28 20656.0 29 23629.0 30 27197.0 31 31752.5 32 37886.5 33 41243.5 34 42252.0 35 45120.5 36 49578.0 37 54035.5 38 57446.0 39 60541.5 40 64225.5 41 68785.5 42 71479.5 43 73802.0 44 77484.0 45 82289.0 46 85730.5 47 88911.0 48 92034.5 49 91792.5 50 90808.0 51 89534.0 52 89222.0 53 90564.5 54 91607.0 55 87914.0 56 81526.5 57 75460.5 58 69625.5 59 62209.0 60 52193.0 61 43932.5 62 37494.0 63 30189.5 64 22747.0 65 17531.5 66 13532.0 67 10501.5 68 8232.0 69 6280.5 70 4879.0 71 3707.0 72 2774.0 73 2155.0 74 1594.0 75 1132.0 76 853.0 77 591.5 78 395.5 79 256.0 80 155.0 81 100.5 82 65.0 83 46.0 84 32.5 85 17.5 86 10.0 87 7.0 88 6.5 89 4.5 90 4.5 91 3.5 92 2.0 93 3.5 94 2.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37729038952835886 2 0.02396837302683894 3 0.04499608852265394 4 0.03782571810454078 5 0.05748381228453641 6 0.05563079521019256 7 0.031742988143107706 8 0.029970537028517936 9 0.03311260945892707 10-11 0.029607990209624572 12-13 0.02936629233036233 14-15 0.023484977268314455 16-17 0.028963462531591926 18-19 0.025056013483519025 20-21 0.037866001084417816 22-23 0.024874740074072345 24-25 0.04662754920767407 26-27 0.039437037299622384 28-29 0.030574781726673533 30-31 0.03810769896368006 32-33 0.03548930527167245 34-35 0.03407940097597604 36-37 0.03452251375462348 38-39 0.016516021749586496 40-41 0.012084893963112069 42-43 0.012064752473173549 44-45 0.010352725828399339 46-47 0.017341822837065823 48-49 0.014381023816103363 50-51 0.009768622620182256 52-53 0.004229712887089224 54-55 0.0043102788468433045 56-57 0.007774615116268764 58-59 0.0031823554102861783 60-61 0.005679900162662672 62-63 0.006223720391002717 64-65 0.0077141906464532044 66-67 0.004390844806597385 68-69 0.0061431544312486345 70-71 0.016455597279770934 72-73 0.013373949319177358 74-75 0.009768622620182256 76-77 0.007391926807436882 78-79 0.006545984230019038 80-81 0.009587349210735575 82-83 0.011299375855509785 84-85 0.0049145235449989085 86-87 4.8339575852448277E-4 88-89 0.023001581509789974 90-91 0.01923512289128671 92-93 0.012628714191452112 94-95 0.023867665577146337 96-97 0.018550312233377025 98-99 0.01699941750811098 100-101 0.10737428286225073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2482438.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.61440212948313 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73512312898683 46.150121687968024 2 12.466236105760247 14.61401952288226 3 3.7928521006519307 6.669472747358012 4 1.6799139270107948 3.938686018429196 5 0.8781335860376386 2.573563756770761 6 0.5392252292502121 1.8963818655380795 7 0.34294412196608964 1.4071025271004023 8 0.24428295827992694 1.145479963999951 9 0.19153409024518583 1.0103990568422432 >10 1.0231242754894472 10.971714663079341 >50 0.07099687574190551 2.808575448701006 >100 0.03086666697174203 3.3547675989188384 >500 0.0029706977557902425 1.2113882566731409 >1k 0.0016580638636925964 1.6873992530661002 >5k 1.381719886410497E-4 0.5609276326727051 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7596 0.3059895151459976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6256 0.2520103221107637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3692 0.14872476170603252 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2974 0.11980158215431766 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2940 0.11843196083849829 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2592 0.10441348384128829 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2573 0.10364810722362451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6113191950816092E-4 2 0.0 0.0 0.0 0.0 1.6113191950816092E-4 3 0.0 0.0 0.0 0.0 1.6113191950816092E-4 4 0.0 0.0 0.0 0.0 1.6113191950816092E-4 5 0.0 0.0 0.0 0.0 2.819808591392816E-4 6 0.0 0.0 0.0 0.0 2.819808591392816E-4 7 0.0 0.0 0.0 0.0 2.819808591392816E-4 8 0.0 0.0 0.0 0.0 2.819808591392816E-4 9 0.0 0.0 0.0 0.0 2.819808591392816E-4 10-11 0.0 0.0 0.0 4.028297987704023E-5 2.819808591392816E-4 12-13 0.0 0.0 0.0 8.056595975408046E-5 5.23678738401523E-4 14-15 0.0 0.0 0.0 1.0070744969260058E-4 5.639617182785632E-4 16-17 0.0 0.0 0.0 1.2084893963112069E-4 6.243861880941236E-4 18-19 0.0 0.0 0.0 1.2084893963112069E-4 7.250936377867242E-4 20-21 0.0 0.0 0.0 1.6113191950816092E-4 0.001027215986864526 22-23 0.0 0.0 0.0 2.819808591392816E-4 0.0011279234365571265 24-25 0.0 0.0 0.0 4.028297987704023E-4 0.0011279234365571265 26-27 0.0 0.0 0.0 7.452351277252444E-4 0.0011883479063726867 28-29 0.0 0.0 0.0 0.0032226383901632185 0.0012487723761882472 30-31 0.0 0.0 0.0 0.010695131157354182 0.0012689138661267673 32-33 0.0 0.0 0.0 0.0278355390950348 0.0013293383359423276 34-35 0.0 0.0 0.0 0.055248106901360686 0.0014904702554504885 36-37 0.0 0.0 0.0 0.1005261762831539 0.0014904702554504885 38-39 0.0 0.0 0.0 0.17003445806098683 0.0014904702554504885 40-41 0.0 0.0 0.0 0.23875722173121744 0.0014904702554504885 42-43 0.0 0.0 0.0 0.30933300247579193 0.0015307532353275289 44-45 0.0 0.0 0.0 0.39719018158761665 0.0016113191950816092 46-47 0.0 0.0 0.0 0.49985135580425366 0.0016918851548356898 48-49 0.0 0.0 0.0 0.6016061629736573 0.00175230962465125 50-51 0.0 0.0 0.0 0.7181045407780577 0.0018127340944668105 52-53 0.0 0.0 0.0 0.8261636342982182 0.0019738660139749713 54-55 0.0 0.0 0.0 0.9409097024779672 0.0020141489938520114 56-57 0.0 0.0 0.0 1.0734608477633683 0.0020342904837905317 58-59 0.0 0.0 0.0 1.2185198583005898 0.002054431973729052 60-61 0.0 0.0 0.0 1.3699637211483227 0.002054431973729052 62-63 0.0 0.0 0.0 1.514217072088004 0.0021752809133601726 64-65 0.0 0.0 0.0 1.6637072104117 0.0022155638932372127 66-67 0.0 0.0 0.0 1.8354335536275226 0.0022155638932372127 68-69 0.0 0.0 0.0 2.0277042165806356 0.0022155638932372127 70-71 0.0 0.0 0.0 2.2272056744216773 0.0022759883630527732 72-73 0.0 0.0 0.0 2.4235046353624945 0.0023565543228068536 74-75 0.0 0.0 0.0 2.6215760474179013 0.002396837302683894 76-77 0.0 0.0 0.0 2.8379359323374844 0.002416978792622414 78-79 0.0 0.0 0.0 3.077700228565628 0.002437120282560934 80-81 0.0 0.0 0.0 3.330616917723625 0.0024774032624379745 82-83 0.0 0.0 0.0 3.5812374770286306 0.0025378277322535346 84-85 0.0 0.0 0.0 3.83493968429423 0.0025982522020690946 86-87 0.0 2.0141489938520115E-5 0.0 4.090374059694542 0.002759384121577256 88-89 0.0 4.028297987704023E-5 0.0 4.358719130145445 0.0029003745511468966 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5810 0.0 37.09941 1 GTATCAA 8215 0.0 30.882242 1 ATCAACG 9580 0.0 25.847122 3 TCAACGC 9700 0.0 25.576363 4 TATCAAC 9855 0.0 25.415226 2 CAACGCA 9850 0.0 25.138624 5 GTACATG 6815 0.0 25.120792 1 TACATGG 6575 0.0 24.79353 2 AACGCAG 10125 0.0 24.544777 6 ACATGGG 6900 0.0 22.385885 3 GAGTACT 7150 0.0 22.058231 12-13 ACGCAGA 11500 0.0 21.602673 7 TAAGGTG 1175 0.0 21.43769 5 CGCAGAG 11625 0.0 21.370386 8 GTACTTT 7615 0.0 20.649723 14-15 GTGTAGC 1790 0.0 19.714394 1 GCAGAGT 12530 0.0 19.257837 9 AGAGTAC 9885 0.0 19.125957 10-11 TAGCCCT 1625 0.0 18.718307 4 GTAAGGT 1145 0.0 18.678717 4 >>END_MODULE