FastQCFastQC Report
Thu 26 May 2016
SRR937915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482438
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.2895137763762881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61690.2485057028614612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37550.15126258943828608No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30710.12370903120239056No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27790.1119464010782948No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26860.10820008394973007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.037.618711
GTATCAA84400.032.737891
ATCAACG99800.027.3021773
TCAACGC100300.027.117574
TATCAAC101950.026.8223952
CAACGCA102500.026.4891455
AACGCAG106600.025.5742386
TACATGG67600.023.2861822
GTACATG70950.023.1251051
TATACCG1503.0102126E-522.1902625
ACATGGG70100.022.1821123
ACGCAGA122300.022.1746967
CGCAGAG122100.022.0943228
GAGTACT78800.021.21604712-13
TAGCCCT17000.020.6984834
GAGTACA52850.020.3366971
GTAGCCC15000.020.289063
GTACTTT83550.019.98144514-15
GTGTAGC18150.019.9137921
GCAGAGT133850.019.7644569
Produced by FastQC (version 0.11.5)