##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937915_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2482438 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39977634889572 33.0 31.0 34.0 31.0 34.0 2 32.639923736262496 34.0 31.0 34.0 31.0 34.0 3 32.71403273717208 34.0 31.0 34.0 31.0 34.0 4 36.18938922140251 37.0 35.0 37.0 35.0 37.0 5 36.10732231781821 37.0 35.0 37.0 35.0 37.0 6 36.157422259891284 37.0 36.0 37.0 35.0 37.0 7 36.127111331682805 37.0 36.0 37.0 35.0 37.0 8 36.11848956549972 37.0 36.0 37.0 35.0 37.0 9 37.79686542020385 39.0 38.0 39.0 35.0 39.0 10-11 37.75973458350219 39.0 38.0 39.0 35.0 39.0 12-13 37.67473749596164 39.0 37.0 39.0 35.0 39.0 14-15 38.896801048002004 40.0 38.0 41.0 35.0 41.0 16-17 38.788783848780916 40.0 38.0 41.0 35.0 41.0 18-19 39.03797778635358 40.0 38.5 41.0 36.0 41.0 20-21 38.99922173282877 40.0 38.5 41.0 35.0 41.0 22-23 38.883521965100435 40.0 38.0 41.0 35.0 41.0 24-25 38.76375381781942 40.0 38.0 41.0 34.5 41.0 26-27 38.58638463478242 40.0 38.0 41.0 34.0 41.0 28-29 38.4546256542963 40.0 38.0 41.0 34.0 41.0 30-31 38.21610912336985 40.0 38.0 41.0 34.0 41.0 32-33 37.9895832242336 40.0 37.0 41.0 33.0 41.0 34-35 37.8997191470643 40.0 37.0 41.0 33.0 41.0 36-37 37.845042857062296 40.0 37.0 41.0 33.0 41.0 38-39 37.64140957397526 40.0 37.0 41.0 33.0 41.0 40-41 37.41210535771689 40.0 37.0 41.0 32.0 41.0 42-43 37.14064681575129 40.0 36.0 41.0 31.0 41.0 44-45 36.86311843437782 39.0 36.0 41.0 31.0 41.0 46-47 36.50045318352362 39.0 35.5 41.0 30.0 41.0 48-49 36.082957358854486 39.0 35.0 40.0 29.5 41.0 50-51 35.674060741899694 38.5 35.0 40.0 28.0 41.0 52-53 35.101540703131356 38.0 34.0 40.0 26.5 41.0 54-55 34.6744514868045 38.0 34.0 40.0 26.0 41.0 56-57 34.250634255518165 38.0 33.0 40.0 24.5 41.0 58-59 33.68495527380745 37.0 33.0 40.0 23.0 41.0 60-61 33.147735210305356 36.5 32.0 39.0 22.0 40.5 62-63 32.53706134856137 36.0 31.0 39.0 19.5 40.0 64-65 31.908087533303952 35.0 31.0 38.5 16.5 40.0 66-67 31.287110695211723 35.0 30.0 38.0 10.5 40.0 68-69 30.59578728653042 34.5 29.0 37.5 7.5 40.0 70-71 29.917718186718055 34.0 28.5 37.0 4.5 39.0 72-73 29.18645903744625 34.0 27.5 36.0 2.0 39.0 74-75 28.483930716497248 33.5 26.0 36.0 2.0 38.5 76-77 27.241099677011068 31.5 25.5 34.5 2.0 36.5 78-79 27.876105868505075 33.0 26.0 35.0 2.0 37.0 80-81 27.56736945696126 33.0 26.0 35.0 2.0 37.0 82-83 26.957605990562506 33.0 25.0 35.0 2.0 36.0 84-85 26.244622423601314 32.0 23.5 35.0 2.0 36.0 86-87 25.463709667673474 31.5 20.5 34.5 2.0 35.0 88-89 24.57959252154535 31.0 17.5 34.0 2.0 35.0 90-91 23.8538201155477 31.0 8.5 34.0 2.0 35.0 92-93 22.832852623106803 30.0 2.0 34.0 2.0 35.0 94-95 21.85038297028969 29.5 2.0 34.0 2.0 35.0 96-97 20.72107500771419 29.0 2.0 34.0 2.0 35.0 98-99 19.230430125545936 26.5 2.0 33.5 2.0 35.0 100-101 15.556061823094876 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21623417087143793 1101 2 0.18896074482460534 1101 3 0.18902769916887507 1101 4 0.06815572885847843 1101 5 0.048678411636508656 1101 6 0.006381242854963887 1101 7 0.08976058790980801 1101 8 0.11253764884931883 1101 9 0.014622668845973408 1101 10-11 0.1138870436543371 1101 12-13 0.20043133258027268 1101 14-15 0.3589971469813804 1101 16-17 0.38887196124589707 1101 18-19 0.3050764639044914 1101 20-21 0.2692695191715657 1101 22-23 0.2563952839358308 1101 24-25 0.34754275974049165 1101 26-27 0.27897252750013024 1101 28-29 0.3518574343517855 1101 30-31 0.491000906633964 1101 32-33 0.4715670245793433 1101 34-35 0.46046800468071325 1101 36-37 0.5939760141530854 1101 38-39 0.6192675326445283 1101 40-41 0.7884746734887997 1101 42-43 0.8523175581333291 1101 44-45 0.7909149576327934 1101 46-47 0.8770468643871396 1101 48-49 1.1266802545241674 1101 50-51 1.0680556334791547 1101 52-53 1.066118393411223 1101 54-55 1.2570911729542402 1101 56-57 1.3164534479122736 1101 58-59 1.6088247107401585 1101 60-61 1.4394978587531142 1101 62-63 1.3360113265718425 1101 64-65 1.6018241429712212 1101 66-67 1.8508653221698346 1101 68-69 2.0646110710036716 1101 70-71 2.2175738888878875 1101 72-73 2.2975688811985364 1101 74-75 2.4668574363115745 1101 76-77 2.331193428124731 1101 78-79 2.7165794611961402 1101 80-81 2.84809793734723 1101 82-83 2.943475403581445 1101 84-85 3.166298559864199 1101 86-87 3.0986438874967064 1101 88-89 3.584535461001032 1101 90-91 3.743739456138762 1101 92-93 3.8512681198991316 1101 94-95 4.182557593092756 1101 96-97 4.330434309937576 1101 98-99 4.609445170806943 1101 100-101 4.482362003692222 1102 1 0.1941491366787247 1102 2 0.1821464324983637 1102 3 0.2273173538991884 1102 4 0.11989047187321944 1102 5 -0.007078105430849746 1102 6 0.0938624599491078 1102 7 0.0918212701461556 1102 8 0.1980440375175334 1102 9 0.20906704969500112 1102 10-11 0.1993150191756996 1102 12-13 0.31074794617020984 1102 14-15 0.4265005639630388 1102 16-17 0.4171947517313086 1102 18-19 0.36650438829514087 1102 20-21 0.26783270795760217 1102 22-23 0.3775952035003485 1102 24-25 0.42435044033683766 1102 26-27 0.42224984980754243 1102 28-29 0.31648795389326523 1102 30-31 0.42491112033744827 1102 32-33 0.4635085951469833 1102 34-35 0.564358269774381 1102 36-37 0.5382806440773678 1102 38-39 0.6253742187866536 1102 40-41 0.657557247302087 1102 42-43 0.7791208888032841 1102 44-45 0.7567869232729549 1102 46-47 0.7665636944049794 1102 48-49 0.8548022234076953 1102 50-51 0.9732523264666995 1102 52-53 1.0670750910386815 1102 54-55 1.313274860425004 1102 56-57 1.4071113513851188 1102 58-59 1.3375128378068482 1102 60-61 1.3297728733101053 1102 62-63 1.5753249120024932 1102 64-65 1.5708378811071846 1102 66-67 1.5421980747498374 1102 68-69 1.8743436688178754 1102 70-71 1.8968772825173055 1102 72-73 2.125898654460535 1102 74-75 2.358367314841786 1102 76-77 2.333285303912202 1102 78-79 2.469496010529266 1102 80-81 2.6221733490448713 1102 82-83 2.660498287246863 1102 84-85 2.9286649427172478 1102 86-87 3.1177830866676253 1102 88-89 3.363476398921474 1102 90-91 3.3868312388225448 1102 92-93 3.4115169852933285 1102 94-95 3.886565134491466 1102 96-97 4.264314856379908 1102 98-99 4.618740181288743 1102 100-101 3.9920622441592055 1103 1 0.21843923133277343 1103 2 0.1781477064025836 1103 3 0.1865872290269479 1103 4 0.12375588241107494 1103 5 -0.022519750248576997 1103 6 0.07588411632085013 1103 7 0.13746407332795485 1103 8 0.16675413543040207 1103 9 0.2465198337682395 1103 10-11 0.27337625545530386 1103 12-13 0.22846484317653193 1103 14-15 0.37626503981069703 1103 16-17 0.3617925090673708 1103 18-19 0.2705768082687783 1103 20-21 0.21358327454872494 1103 22-23 0.2962119805226777 1103 24-25 0.3866947203587614 1103 26-27 0.38740634745970937 1103 28-29 0.44985165354812295 1103 30-31 0.48713245378928605 1103 32-33 0.6485383689549167 1103 34-35 0.6420253215748843 1103 36-37 0.5750213008786886 1103 38-39 0.6380825243459043 1103 40-41 0.6864319158647945 1103 42-43 0.8040475652464281 1103 44-45 0.848498028458927 1103 46-47 0.9009361484481531 1103 48-49 0.9764767408794341 1103 50-51 1.0383880861431791 1103 52-53 1.2215452357601322 1103 54-55 1.257938534898038 1103 56-57 1.3371158693012504 1103 58-59 1.488554921084635 1103 60-61 1.6294040335998474 1103 62-63 1.7560756267220086 1103 64-65 1.727024833957909 1103 66-67 1.8899057877955094 1103 68-69 1.986827930175611 1103 70-71 2.132016301018542 1103 72-73 2.393949951324654 1103 74-75 2.4726235621569614 1103 76-77 2.414534100368968 1103 78-79 2.443519474067461 1103 80-81 2.634117312072025 1103 82-83 2.842329968948558 1103 84-85 3.0725976035476563 1103 86-87 3.2455364232597823 1103 88-89 3.266585613989097 1103 90-91 3.577750227401843 1103 92-93 3.692770225602029 1103 94-95 4.0180231623099765 1103 96-97 4.035653122388883 1103 98-99 4.355913743332319 1103 100-101 4.097830734286452 1104 1 0.27830967667690487 1104 2 0.25996327095499083 1104 3 0.2456506568272232 1104 4 0.1902723016643364 1104 5 0.1253174067464755 1104 6 0.16716976634334202 1104 7 0.22610914755197342 1104 8 0.2328252105541111 1104 9 0.2689597797871315 1104 10-11 0.2958323958061868 1104 12-13 0.3376776187050936 1104 14-15 0.41383049190246624 1104 16-17 0.4100768095621987 1104 18-19 0.42433299360390464 1104 20-21 0.4153363109859711 1104 22-23 0.3918417600998154 1104 24-25 0.5160127590452248 1104 26-27 0.5824108458852564 1104 28-29 0.5964058595760093 1104 30-31 0.515346129002964 1104 32-33 0.6749094890628768 1104 34-35 0.6353469590017866 1104 36-37 0.7394477516209292 1104 38-39 0.8285966943863912 1104 40-41 0.9815173859502693 1104 42-43 0.867930156339547 1104 44-45 0.9293522794710753 1104 46-47 1.087009922627189 1104 48-49 1.2180210503711137 1104 50-51 1.3039494896519557 1104 52-53 1.3131583711627428 1104 54-55 1.4103586878445071 1104 56-57 1.4963430397915758 1104 58-59 1.6496493880212029 1104 60-61 1.6863541010762333 1104 62-63 1.8061156627094093 1104 64-65 1.976752229369513 1104 66-67 2.2109561701616762 1104 68-69 2.2665710700766546 1104 70-71 2.333102220946568 1104 72-73 2.637695790281022 1104 74-75 2.4902303997637993 1104 76-77 2.697504860602887 1104 78-79 2.6810012554439773 1104 80-81 2.817871698036935 1104 82-83 2.981591327473076 1104 84-85 3.309096029098715 1104 86-87 3.418245824969187 1104 88-89 3.3846009086359707 1104 90-91 3.6429270155259985 1104 92-93 3.7858246565511955 1104 94-95 4.0387492081939165 1104 96-97 4.3368663477600045 1104 98-99 4.811938484672851 1104 100-101 4.176784400503275 1105 1 0.17804911919909472 1105 2 0.1612662302724317 1105 3 0.2070859150421711 1105 4 0.0925879192096275 1105 5 0.002800718946943448 1105 6 0.01646129242272565 1105 7 0.12705741859708297 1105 8 0.12208458602422212 1105 9 0.2137267523430637 1105 10-11 0.15694778124814945 1105 12-13 0.17743476985165074 1105 14-15 0.4187460105889329 1105 16-17 0.27907184512987726 1105 18-19 0.19045235488993484 1105 20-21 0.21201198956584477 1105 22-23 0.23113771333529343 1105 24-25 0.2193926993001014 1105 26-27 0.2481575523820041 1105 28-29 0.31165203954205367 1105 30-31 0.31878753365791823 1105 32-33 0.5441438489519825 1105 34-35 0.49921646535238295 1105 36-37 0.46265937398021606 1105 38-39 0.5395799958413363 1105 40-41 0.5270053402173218 1105 42-43 0.538191014692039 1105 44-45 0.7005734438561078 1105 46-47 0.7578847372668918 1105 48-49 0.7675317851500765 1105 50-51 0.9437746384774712 1105 52-53 1.0998611714464204 1105 54-55 1.238044316780055 1105 56-57 1.1511674794124716 1105 58-59 1.239728346135145 1105 60-61 1.425938464188988 1105 62-63 1.4348963035724935 1105 64-65 1.487663922649265 1105 66-67 1.6699374944401164 1105 68-69 1.962888191995365 1105 70-71 2.2204502684847647 1105 72-73 2.1407177756241254 1105 74-75 2.1155795218544498 1105 76-77 2.025413824519415 1105 78-79 2.435021500012116 1105 80-81 2.6689818826450455 1105 82-83 2.7286133987803787 1105 84-85 3.0222508721066 1105 86-87 2.952625571804628 1105 88-89 3.035432483285131 1105 90-91 3.2846059822363642 1105 92-93 3.439132773720395 1105 94-95 3.5282717200677247 1105 96-97 3.8735411666979154 1105 98-99 3.9558426345944113 1105 100-101 3.6818432215043284 1106 1 0.13985641634199197 1106 2 0.1610088878415752 1106 3 0.09082305144643499 1106 4 0.11680349352045027 1106 5 0.06322591633821162 1106 6 0.09159449741115822 1106 7 0.1420426790646232 1106 8 0.21720023996021354 1106 9 0.24859424587921808 1106 10-11 0.14638180875341789 1106 12-13 0.18424961553993313 1106 14-15 0.10652999360267756 1106 16-17 0.13631158789364406 1106 18-19 0.1121299952506476 1106 20-21 0.14090409472814258 1106 22-23 0.14909533695134058 1106 24-25 0.1036538121045254 1106 26-27 -0.006052551535560724 1106 28-29 0.18577218477543767 1106 30-31 0.29425389007569436 1106 32-33 0.3940355610490158 1106 34-35 0.3128072981878205 1106 36-37 0.3548616826288651 1106 38-39 0.33813487954860477 1106 40-41 0.37489406209817844 1106 42-43 0.4134253978973206 1106 44-45 0.5385310044224241 1106 46-47 0.6625539676238787 1106 48-49 0.6153087596891069 1106 50-51 0.9391567438703916 1106 52-53 0.9672795456959804 1106 54-55 1.0233328746320751 1106 56-57 0.9791186337948687 1106 58-59 1.1846543282466158 1106 60-61 1.1241393402815163 1106 62-63 1.2792863464280586 1106 64-65 1.4016682455375253 1106 66-67 1.6234444611858656 1106 68-69 1.6247705183112124 1106 70-71 1.6808125654791688 1106 72-73 2.0259167530903177 1106 74-75 2.2129935041928945 1106 76-77 2.2750289308353544 1106 78-79 2.4685421352745998 1106 80-81 2.1657237051371716 1106 82-83 2.5507090588189136 1106 84-85 2.870301615189092 1106 86-87 3.0602385108692296 1106 88-89 3.0144714362349347 1106 90-91 3.109934351012214 1106 92-93 3.1683204327654018 1106 94-95 3.475103877883175 1106 96-97 3.78880980249599 1106 98-99 3.5718318955670085 1106 100-101 3.341573810028711 1107 1 0.25070410113345787 1107 2 0.22545643078866817 1107 3 0.20404107413122574 1107 4 0.10883955073256857 1107 5 0.043998751943277625 1107 6 0.10244106780616846 1107 7 0.17804417135791084 1107 8 0.1885848675763171 1107 9 0.17979124239781186 1107 10-11 0.21220829256814255 1107 12-13 0.2845220937896542 1107 14-15 0.4602653756734938 1107 16-17 0.3809615285229171 1107 18-19 0.25139204524447223 1107 20-21 0.2565262198555658 1107 22-23 0.32209274573008173 1107 24-25 0.41686167215895154 1107 26-27 0.331479506074416 1107 28-29 0.45149860576813694 1107 30-31 0.5159584908531443 1107 32-33 0.550164417214198 1107 34-35 0.49036524971423745 1107 36-37 0.5416176462715967 1107 38-39 0.5341064860575528 1107 40-41 0.5256434604488405 1107 42-43 0.5993961278586042 1107 44-45 0.7377790196740222 1107 46-47 0.7941330025332576 1107 48-49 0.8559724044008874 1107 50-51 0.8529773889130041 1107 52-53 0.945191376311719 1107 54-55 0.9467845662942125 1107 56-57 1.0666222909280094 1107 58-59 1.2546765978796586 1107 60-61 1.3921127670822244 1107 62-63 1.4399016462930163 1107 64-65 1.5637251918617103 1107 66-67 1.733265237960108 1107 68-69 1.9658778757728363 1107 70-71 1.8988269338750179 1107 72-73 2.132740967694467 1107 74-75 2.08841296218705 1107 76-77 1.9796089173062477 1107 78-79 1.8899960633807247 1107 80-81 2.099808402261683 1107 82-83 2.4261824901936286 1107 84-85 2.4241025137918193 1107 86-87 2.6258070202209254 1107 88-89 2.8669071326487234 1107 90-91 2.8635799659536474 1107 92-93 3.1115579008366936 1107 94-95 3.253987245981797 1107 96-97 3.519166194798803 1107 98-99 3.5926724399047707 1107 100-101 3.069973189093661 1108 1 0.23684702441305916 1108 2 0.186873866220445 1108 3 0.16663985471276277 1108 4 0.13194272225730685 1108 5 0.0565736696745347 1108 6 0.12815896422888073 1108 7 0.1486106414614241 1108 8 0.13199505656651667 1108 9 0.20042197238653614 1108 10-11 0.1675538391525393 1108 12-13 0.21718577774900183 1108 14-15 0.3731970742973161 1108 16-17 0.23935008007472192 1108 18-19 0.2417427844565836 1108 20-21 0.29195915630888436 1108 22-23 0.35630510186033604 1108 24-25 0.3487781003460739 1108 26-27 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84-85 2.5002640778379224 2316 86-87 2.667118993958109 2316 88-89 3.0860815878259054 2316 90-91 3.2958775805774287 2316 92-93 3.5442831285618794 2316 94-95 3.899531668095012 2316 96-97 4.229401576507112 2316 98-99 4.616654529876968 2316 100-101 4.816443150808835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 23.0 5 39.0 6 100.0 7 268.0 8 674.0 9 1231.0 10 2178.0 11 3649.0 12 5569.0 13 8483.0 14 11427.0 15 15624.0 16 19762.0 17 26259.0 18 27685.0 19 25320.0 20 25992.0 21 27537.0 22 30481.0 23 34367.0 24 37870.0 25 42457.0 26 48026.0 27 55187.0 28 65097.0 29 77452.0 30 91892.0 31 108322.0 32 129204.0 33 160420.0 34 201300.0 35 264449.0 36 349851.0 37 395217.0 38 182724.0 39 6296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.527371076337054 18.370932123984566 14.067662515639867 24.034034284038512 2 16.142066168676582 19.517741148562656 39.35162448415885 24.988568198601914 3 20.068756984264205 22.490962251336025 29.20584635786987 28.2344344065299 4 13.260463051512206 15.90870455309011 35.74763475053597 35.08319764486171 5 14.034013687746766 36.844921942740534 34.223399836263255 14.897664533249449 6 34.1536827908693 35.16853190291157 16.332129946447807 14.345655359771323 7 29.896335779584426 31.712896757139553 19.664217192936945 18.72655027033908 8 26.78223626939582 35.325602464360514 18.845372818316253 19.046788447927412 9 26.014627555652954 16.504299402442278 19.689676036219232 37.79139700568554 10-11 25.632382359599713 26.014285150323996 26.64562015244691 21.707712337629378 12-13 26.198821481140715 23.057454002879428 27.31276672368051 23.430957792299345 14-15 22.63303252689493 24.88879883404943 25.364359552987832 27.113809086067807 16-17 22.691744970065717 27.983679753532613 26.38116238955414 22.94341288684753 18-19 22.659256746794885 26.61514607816993 28.2713606543245 22.454236520710687 20-21 22.929434692830196 25.793413571658185 28.75350764047279 22.52364409503883 22-23 22.667227138548004 25.77712098458286 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22.741454167233986 48-49 22.9443393953847 26.517862681766875 27.63980409581226 22.897993827036164 50-51 22.902062408003747 26.75259160551039 27.693944420767004 22.651401565718864 52-53 23.16208904311004 26.580120027166842 27.518693316811945 22.739097612911177 54-55 22.691765111555657 26.675912953314445 27.65982473681115 22.97249719831875 56-57 22.860539864657188 26.83210087506752 27.48627659030219 22.821082669973094 58-59 22.885526244764222 26.501326518527353 27.45172286276636 23.16142437394207 60-61 22.602759867517335 26.785543083049806 27.665182373134794 22.946514676298058 62-63 22.5926488395682 26.789712371467083 27.77432910711164 22.843309681853082 64-65 22.391395072102505 26.807195184733718 27.63505070418677 23.166359038977006 66-67 22.39856995392633 26.838540748760035 27.472031017900754 23.290858279412877 68-69 22.755665144091424 26.458528119973394 27.691640848415073 23.094165887520106 70-71 22.64705100389214 26.59893217876942 27.334297976424786 23.41971884091365 72-73 22.66799627462702 26.660586617338776 27.313775662293004 23.3576414457412 74-75 22.569788248487978 27.027966055949836 27.080897891508265 23.321347804053918 76-77 22.66958933113335 26.64233708958693 27.386967972614016 23.301105606665708 78-79 22.650696613571014 26.76920833470967 27.417361480931245 23.16273357078807 80-81 22.855025697535467 26.756575646547255 27.2568301344264 23.131568521490884 82-83 22.7249088078247 26.889374978599296 27.249954177316667 23.13576203625934 84-85 22.633791108798768 26.958114562102026 27.16519818775139 23.242896141347817 86-87 22.799596433809082 26.876529411326324 27.092432086533485 23.231442068331113 88-89 22.603746800524323 27.114111208416887 26.764736923943317 23.517405067115472 90-91 22.66223595818838 26.913593127863187 27.00981158343713 23.4143593305113 92-93 22.74644925673874 26.616455275015934 27.11562182016228 23.521473648083056 94-95 22.471612063390143 26.518684365138846 27.244805657667374 23.764897913803637 96-97 22.662835484412188 26.278114263949952 27.186153603526215 23.872896648111645 98-99 22.43302753180543 26.497439210969215 26.879966387881595 24.189566869343764 100-101 22.884942141556003 25.76338261015985 27.02512610586851 24.32654914241564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 467.0 1 308.5 2 252.5 3 387.5 4 322.5 5 283.0 6 596.0 7 1220.5 8 1866.0 9 2199.5 10 1982.5 11 1687.5 12 1716.5 13 1850.0 14 2052.5 15 2257.5 16 2435.5 17 2671.0 18 2997.0 19 3303.5 20 3757.5 21 4541.0 22 5597.0 23 7021.5 24 8917.0 25 11230.0 26 14053.0 27 17477.5 28 20637.5 29 23678.5 30 27305.5 31 31624.5 32 38090.0 33 41769.0 34 42543.5 35 45477.0 36 49667.0 37 53874.0 38 57737.5 39 61374.5 40 65005.0 41 69275.5 42 72479.0 43 75126.0 44 78613.0 45 83865.0 46 87435.5 47 90491.0 48 93933.0 49 94016.0 50 92288.0 51 90556.5 52 90433.5 53 92190.5 54 93502.0 55 88672.5 56 81219.5 57 74935.0 58 69179.0 59 61374.5 60 50598.0 61 42028.5 62 35679.0 63 28045.5 64 20603.0 65 15545.0 66 11660.5 67 9091.5 68 7070.5 69 5451.5 70 4197.5 71 2992.5 72 2167.5 73 1704.5 74 1295.5 75 942.5 76 687.5 77 430.0 78 272.0 79 163.0 80 86.0 81 56.5 82 34.0 83 14.5 84 12.5 85 9.5 86 5.0 87 4.5 88 2.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013172534419792157 3 0.0029003745511468966 4 0.0014501872755734483 5 0.11472592668981059 6 0.0 7 0.0 8 3.625468188933621E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.4169787926224138E-4 24-25 5.035372484630029E-4 26-27 0.0062438618809412365 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01043329178815342 36-37 5.841032082170833E-4 38-39 0.006787682109281278 40-41 0.010090886459198577 42-43 0.005559051223031552 44-45 0.0035046192493025 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.028297987704023E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0141489938520115E-5 68-69 8.459425774178448E-4 70-71 0.0 72-73 8.056595975408046E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.0424469815560346E-5 82-83 0.00173216813471273 84-85 0.004330420336781825 86-87 0.0027996671014542964 88-89 0.0 90-91 0.002658676671884655 92-93 0.0 94-95 6.0424469815560346E-5 96-97 6.0424469815560346E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2482438.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.48683616466956 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96757775453045 44.82109368530827 2 12.914454527361693 14.848222631410335 3 3.9110778010273592 6.745064663248078 4 1.716036536829649 3.9459804498125193 5 0.9173736503816307 2.636845437063683 6 0.5662717559104736 1.9531902994023094 7 0.370287795798377 1.490067169558654 8 0.25874793600910184 1.1899680164241313 9 0.18700598676823277 0.9675344270841985 >10 1.0759885452333469 11.367436993027576 >50 0.07702854524339617 3.027268273722614 >100 0.033449293933507165 3.5689356194971094 >500 0.0028058931180276756 1.1619383661084466 >1k 0.0017536831987660507 1.7378668642251152 >5k 1.4029465590128406E-4 0.5385871041068925 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7187 0.2895137763762881 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6169 0.2485057028614612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3755 0.15126258943828608 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3071 0.12370903120239056 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2779 0.1119464010782948 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2686 0.10820008394973007 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2084893963112069E-4 2 0.0 0.0 0.0 0.0 1.2084893963112069E-4 3 0.0 0.0 0.0 0.0 1.2084893963112069E-4 4 0.0 0.0 0.0 0.0 1.2084893963112069E-4 5 0.0 0.0 0.0 0.0 1.6113191950816092E-4 6 0.0 0.0 0.0 0.0 1.6113191950816092E-4 7 0.0 0.0 0.0 0.0 1.6113191950816092E-4 8 0.0 0.0 0.0 0.0 1.6113191950816092E-4 9 0.0 0.0 0.0 0.0 1.6113191950816092E-4 10-11 0.0 0.0 0.0 0.0 1.6113191950816092E-4 12-13 0.0 0.0 0.0 4.028297987704023E-5 5.035372484630029E-4 14-15 0.0 2.0141489938520115E-5 0.0 6.0424469815560346E-5 5.639617182785633E-4 16-17 0.0 4.028297987704023E-5 0.0 8.056595975408046E-5 6.243861880941236E-4 18-19 0.0 4.028297987704023E-5 0.0 8.056595975408046E-5 6.445276780326437E-4 20-21 0.0 4.028297987704023E-5 0.0 1.2084893963112069E-4 8.459425774178448E-4 22-23 0.0 4.028297987704023E-5 0.0 2.4169787926224138E-4 8.862255572948851E-4 24-25 0.0 4.028297987704023E-5 0.0 3.625468188933621E-4 8.862255572948851E-4 26-27 0.0 4.028297987704023E-5 0.0 7.049521478482041E-4 9.063670472334053E-4 28-29 0.0 4.028297987704023E-5 0.0 0.003142072430409138 9.466500271104455E-4 30-31 0.0 4.028297987704023E-5 0.0 0.010795838607046783 9.869330069874856E-4 32-33 0.0 4.028297987704023E-5 0.0 0.02799667101454296 0.0010876404566800863 34-35 0.0 4.028297987704023E-5 0.0 0.055590512230315525 0.0011279234365571265 36-37 0.0 4.028297987704023E-5 0.0 0.10066716671272354 0.0011279234365571265 38-39 0.0 8.056595975408046E-5 0.0 0.16983304316160164 0.0011279234365571265 40-41 0.0 8.056595975408046E-5 0.0 0.23873708024127893 0.0011883479063726867 42-43 0.0 8.056595975408046E-5 0.0 0.30945385141542303 0.0012286308862497271 44-45 0.0 8.056595975408046E-5 0.0 0.3975930113863871 0.0012890553560652874 46-47 0.0 8.056595975408046E-5 0.0 0.5007778643414257 0.0013696213158193677 48-49 0.0 8.056595975408046E-5 0.0 0.6028750768397841 0.0014904702554504885 50-51 0.0 8.056595975408046E-5 0.0 0.7190310493152297 0.0015307532353275289 52-53 0.0 8.056595975408046E-5 0.0 0.8268283034661893 0.0016918851548356898 54-55 0.0 8.056595975408046E-5 0.0 0.9418966354849547 0.0016918851548356898 56-57 0.0 8.056595975408046E-5 0.0 1.0741859414011548 0.0017120266447742099 58-59 0.0 8.056595975408046E-5 0.0 1.220070753025856 0.00173216813471273 60-61 0.0 8.056595975408046E-5 0.0 1.3716153233232813 0.0017724511145897702 62-63 0.0 8.056595975408046E-5 0.0 1.5156672593635774 0.001893300054220891 64-65 0.0 8.056595975408046E-5 0.0 1.6653789540765973 0.0020342904837905317 66-67 0.0 8.056595975408046E-5 0.0 1.8378303909302065 0.002074573463667572 68-69 0.0 1.0070744969260058E-4 0.0 2.0305240251720287 0.0020947149536060922 70-71 0.0 1.2084893963112069E-4 0.0 2.23125411389932 0.0021349979334831324 72-73 0.0 1.2084893963112069E-4 0.0 2.428942837645895 0.0023364128328683337 74-75 0.0 1.2084893963112069E-4 0.0 2.6291693891247236 0.0025781107121305748 76-77 0.0 1.2084893963112069E-4 0.0 2.84806710177656 0.002618393692007615 78-79 0.0 1.2084893963112069E-4 0.0 3.0915575736433296 0.0026385351819461353 80-81 0.0 1.2084893963112069E-4 0.0 3.3492679374066947 0.0027392426316387355 82-83 0.0 1.2084893963112069E-4 0.0 3.606615754351166 0.0029003745511468966 84-85 0.0 1.2084893963112069E-4 0.0 3.866199276678813 0.002920516041085417 86-87 0.0 1.2084893963112069E-4 0.0 4.127978221409759 0.0030413649807165375 88-89 0.0 1.2084893963112069E-4 0.0 4.405548094252505 0.003142072430409138 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5790 0.0 37.61871 1 GTATCAA 8440 0.0 32.73789 1 ATCAACG 9980 0.0 27.302177 3 TCAACGC 10030 0.0 27.11757 4 TATCAAC 10195 0.0 26.822395 2 CAACGCA 10250 0.0 26.489145 5 AACGCAG 10660 0.0 25.574238 6 TACATGG 6760 0.0 23.286182 2 GTACATG 7095 0.0 23.125105 1 TATACCG 150 3.0102126E-5 22.190262 5 ACATGGG 7010 0.0 22.182112 3 ACGCAGA 12230 0.0 22.174696 7 CGCAGAG 12210 0.0 22.094322 8 GAGTACT 7880 0.0 21.216047 12-13 TAGCCCT 1700 0.0 20.698483 4 GAGTACA 5285 0.0 20.336697 1 GTAGCCC 1500 0.0 20.28906 3 GTACTTT 8355 0.0 19.981445 14-15 GTGTAGC 1815 0.0 19.913792 1 GCAGAGT 13385 0.0 19.764456 9 >>END_MODULE