FastQCFastQC Report
Thu 26 May 2016
SRR937914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433305
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73670.30275694990969076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61430.25245499433897517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36500.15000174659567955No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30160.12394664869385466No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28520.11720684418928165No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24340.10002856197640657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.038.5707321
GTATCAA79950.037.074581
ATCAACG92750.031.413993
TCAACGC93450.031.2804034
TATCAAC94450.030.9989052
CAACGCA94600.030.84995
AACGCAG98200.029.7618376
ACGCAGA111450.026.1285767
CGCAGAG111900.025.9385958
GTACATG65750.024.8275241
TACATGG64150.024.0798782
GCAGAGT124650.023.0934549
ACATGGG65600.022.8960083
GAGTACT73300.022.6813112-13
GTCGTAA1302.847723E-422.0298211
ACTACGC1100.00287667521.5929838
AGAGTAC98850.020.90252910-11
GTACTTT79400.020.82235314-15
TAAGGTG12650.020.665635
GTGTAAG11800.020.2251191
Produced by FastQC (version 0.11.5)