FastQCFastQC Report
Thu 26 May 2016
SRR937914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433305
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.294907543443999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61660.2534002108243726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37730.15505659997410928No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29270.1202890718590559No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27940.11482325479132292No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25240.10372723518013566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55600.033.6605871
GTATCAA78950.031.045441
ATCAACG95850.025.3712833
TCAACGC96650.025.2093834
GTGTACG2451.0913936E-1125.2044681
TATCAAC97350.024.9824072
CAACGCA97550.024.9281145
AACGCAG101850.023.9223086
TACATGG64350.023.3259222
GTACATG67000.023.1122931
CGCAGAG115750.021.0495648
ACGCAGA116300.020.9908567
ACATGGG68700.020.7409733
GAGTACT74850.020.5906312-13
AGAGTAC101200.019.68784510-11
GTACTTT78800.019.28725814-15
GTGTAGC15850.019.1801051
GCAGAGT129500.018.7045449
AGTACTT79500.018.69923812-13
AGGCGAC8200.018.5345845
Produced by FastQC (version 0.11.5)