##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937914_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2433305 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.412107401250566 33.0 31.0 34.0 31.0 34.0 2 32.65478392556626 34.0 31.0 34.0 31.0 34.0 3 32.62878184198035 34.0 31.0 34.0 31.0 34.0 4 36.16882717127528 37.0 35.0 37.0 35.0 37.0 5 36.121146753078634 37.0 35.0 37.0 35.0 37.0 6 36.18146800339456 37.0 36.0 37.0 35.0 37.0 7 36.137692973137355 37.0 36.0 37.0 35.0 37.0 8 36.129539042577896 37.0 36.0 37.0 35.0 37.0 9 37.8269666153647 39.0 38.0 39.0 35.0 39.0 10-11 37.7835201505771 39.0 38.0 39.0 35.0 39.0 12-13 37.72096387423689 39.0 38.0 39.0 35.0 39.0 14-15 38.98227451963482 40.0 38.0 41.0 35.5 41.0 16-17 38.90489108434824 40.0 38.0 41.0 35.0 41.0 18-19 38.872637626602504 40.0 38.0 41.0 35.0 41.0 20-21 38.86363834373414 40.0 38.0 41.0 35.0 41.0 22-23 38.89808121053464 40.0 38.0 41.0 35.0 41.0 24-25 38.84066239127442 40.0 38.0 41.0 35.0 41.0 26-27 38.659849874964294 40.0 38.0 41.0 34.0 41.0 28-29 38.58065552818081 40.0 38.0 41.0 34.0 41.0 30-31 38.37082013968656 40.0 38.0 41.0 34.0 41.0 32-33 38.17894119315088 40.0 38.0 41.0 34.0 41.0 34-35 37.9682731922221 40.0 37.5 41.0 33.0 41.0 36-37 37.891854083232474 40.0 37.0 41.0 33.0 41.0 38-39 37.84478312418706 40.0 37.0 41.0 33.0 41.0 40-41 37.72120860311387 40.0 37.0 41.0 33.0 41.0 42-43 37.533770530204805 40.0 37.0 41.0 33.0 41.0 44-45 37.29569762113668 40.0 37.0 41.0 32.0 41.0 46-47 36.95841807747077 40.0 36.0 41.0 31.0 41.0 48-49 36.59189990568383 39.0 36.0 41.0 30.5 41.0 50-51 36.28249623454519 39.0 35.0 40.5 30.0 41.0 52-53 35.78638847164659 39.0 35.0 40.0 28.5 41.0 54-55 35.445428337179266 38.0 35.0 40.0 28.0 41.0 56-57 35.14679849011941 38.0 34.0 40.0 27.0 41.0 58-59 34.736665974877795 38.0 34.0 40.0 26.5 41.0 60-61 34.281066491870114 37.0 33.5 40.0 26.0 41.0 62-63 33.81542243985032 36.5 33.0 39.5 25.0 41.0 64-65 33.35080661898118 36.0 32.5 39.0 23.5 40.0 66-67 32.797781823486986 35.5 32.0 39.0 22.0 40.0 68-69 32.22269197655041 35.0 31.0 38.0 20.5 40.0 70-71 31.590985511475132 35.0 30.5 37.5 18.5 39.5 72-73 31.09260737967497 34.5 30.0 37.0 17.5 39.0 74-75 30.507332825108236 34.0 29.5 36.0 13.0 39.0 76-77 29.356502575714924 32.5 28.5 35.0 13.0 37.0 78-79 30.056385040099784 34.0 29.5 35.0 11.0 37.0 80-81 29.84116993143071 34.0 29.5 35.0 7.5 37.0 82-83 29.338480585047908 34.0 29.0 35.0 4.5 36.5 84-85 28.781143547561854 34.0 29.0 35.0 2.0 36.0 86-87 28.18022052311568 33.0 27.5 35.0 2.0 36.0 88-89 27.479305512461444 33.0 26.0 35.0 2.0 35.0 90-91 26.87729137942017 32.5 25.0 35.0 2.0 35.0 92-93 26.158789177682205 32.0 24.5 34.0 2.0 35.0 94-95 25.31511236774675 31.5 21.5 34.0 2.0 35.0 96-97 24.379718325487353 31.0 14.0 34.0 2.0 35.0 98-99 23.231657354914404 31.0 2.0 34.0 2.0 35.0 100-101 19.728334508004544 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09356282213515499 1101 2 0.016148619010486698 1101 3 0.17415049781767777 1101 4 0.0018128789071667484 1101 5 0.04384540260473102 1101 6 0.02500939806200364 1101 7 0.010332980745239695 1101 8 0.08566566256730823 1101 9 0.06602617396013954 1101 10-11 0.13444307120563792 1101 12-13 0.03823104133766719 1101 14-15 0.17959174411214462 1101 16-17 0.2759063369292605 1101 18-19 0.2906812772152563 1101 20-21 0.28226727296025445 1101 22-23 0.15523231767231493 1101 24-25 0.23031023493843605 1101 26-27 0.30981266452023704 1101 28-29 0.2094253017543437 1101 30-31 0.2018009380955732 1101 32-33 0.2870486238654806 1101 34-35 0.22923848159958027 1101 36-37 0.3694655326302936 1101 38-39 0.38176280725221545 1101 40-41 0.44609003017997395 1101 42-43 0.45839654680573716 1101 44-45 0.49894899448370467 1101 46-47 0.3964954045127911 1101 48-49 0.4662048597626196 1101 50-51 0.6696397324612562 1101 52-53 0.6284862747228601 1101 54-55 0.7508384017568019 1101 56-57 0.7297429506889443 1101 58-59 0.803449451497606 1101 60-61 0.8300377575406301 1101 62-63 0.8229167116725122 1101 64-65 0.7418746146694062 1101 66-67 0.9229657108122282 1101 68-69 0.9392997079033876 1101 70-71 0.902050474224005 1101 72-73 1.0141909240356597 1101 74-75 0.9230194261287004 1101 76-77 0.9345954049752585 1101 78-79 1.0975342461338613 1101 80-81 1.1242333919006349 1101 82-83 1.313674168554737 1101 84-85 1.416953936966415 1101 86-87 1.6971649164137688 1101 88-89 1.9192298116542155 1101 90-91 2.11101243250792 1101 92-93 2.475760955562844 1101 94-95 2.4271964161455806 1101 96-97 2.599519790375485 1101 98-99 2.8614961142271653 1101 100-101 3.367486330415094 1102 1 0.09707522808406566 1102 2 0.16367640598352295 1102 3 0.23927640364149738 1102 4 0.15507176889948227 1102 5 0.0979371825551354 1102 6 0.09509400534807355 1102 7 0.12493810729200305 1102 8 0.14079222753625942 1102 9 0.08432874051103312 1102 10-11 0.22029280942305007 1102 12-13 0.20149127511204767 1102 14-15 0.25790610608333253 1102 16-17 0.29688417690703517 1102 18-19 0.2981164160599903 1102 20-21 0.36172803761958505 1102 22-23 0.38662448680968 1102 24-25 0.26049789829080794 1102 26-27 0.29622389606189614 1102 28-29 0.2705590095258046 1102 30-31 0.21863672465053696 1102 32-33 0.3092157473816357 1102 34-35 0.2503500624881667 1102 36-37 0.42856481263259383 1102 38-39 0.4225278380372117 1102 40-41 0.4017719886494646 1102 42-43 0.3367503118172266 1102 44-45 0.46058456297452466 1102 46-47 0.3710346493218424 1102 48-49 0.3827381496870572 1102 50-51 0.4121424118315815 1102 52-53 0.48422007258962907 1102 54-55 0.3957974346124544 1102 56-57 0.44048484948577027 1102 58-59 0.5602306346157704 1102 60-61 0.663083761864776 1102 62-63 0.5801867911086092 1102 64-65 0.5691949785029422 1102 66-67 0.6461788357391711 1102 68-69 0.836969975833135 1102 70-71 1.1144282182797767 1102 72-73 1.0652901486843405 1102 74-75 1.0690645007304553 1102 76-77 1.1211922896587296 1102 78-79 1.3940425795855518 1102 80-81 1.3787997927606561 1102 82-83 1.3446953227433518 1102 84-85 1.4865377214135798 1102 86-87 1.5671595558522782 1102 88-89 1.630550192239511 1102 90-91 1.955554055337796 1102 92-93 2.0163350709700545 1102 94-95 1.9572795631156445 1102 96-97 2.324702976275173 1102 98-99 2.419035113950759 1102 100-101 2.786071072989788 1103 1 0.3003120960508596 1103 2 0.28058978529012535 1103 3 0.3706843549896419 1103 4 0.1755655773678555 1103 5 0.14040357996179154 1103 6 0.12502780252640378 1103 7 0.2112590257854734 1103 8 0.21993233386638877 1103 9 0.2451875313457279 1103 10-11 0.30458107651852373 1103 12-13 0.30541409721118384 1103 14-15 0.39675644247142117 1103 16-17 0.28100042975349027 1103 18-19 0.38780168278955074 1103 20-21 0.4966609726238147 1103 22-23 0.4250035013516822 1103 24-25 0.4430393165274964 1103 26-27 0.48530557081991077 1103 28-29 0.4752576014015588 1103 30-31 0.5528942665711298 1103 32-33 0.5311152968547717 1103 34-35 0.4564608441506337 1103 36-37 0.38701409553271304 1103 38-39 0.4444241397542186 1103 40-41 0.5361852826732658 1103 42-43 0.4694211496308256 1103 44-45 0.6447884117758207 1103 46-47 0.6528357894477992 1103 48-49 0.7284872574887302 1103 50-51 0.7662310096841338 1103 52-53 0.6641491500072618 1103 54-55 0.8116844061569708 1103 56-57 0.7607835823001139 1103 58-59 0.7370457214009534 1103 60-61 0.9316127947016852 1103 62-63 1.002916364241301 1103 64-65 0.96292162211288 1103 66-67 0.8937990950082408 1103 68-69 1.0651719245802767 1103 70-71 1.059212158382735 1103 72-73 1.1249806351888623 1103 74-75 0.9368704288433598 1103 76-77 0.6641833515534827 1103 78-79 1.100228246560171 1103 80-81 1.2650695649364074 1103 82-83 1.4146498868687765 1103 84-85 1.5042260760816326 1103 86-87 1.8179571253157079 1103 88-89 1.8658143750036018 1103 90-91 1.7413942124637707 1103 92-93 1.7874196673066756 1103 94-95 1.641506309765898 1103 96-97 2.119331351196138 1103 98-99 2.8526596238082824 1103 100-101 2.9771875020094924 1104 1 0.12596861861233322 1104 2 0.153718371042892 1104 3 0.17011193001678038 1104 4 0.1143243962793008 1104 5 0.045045766853192504 1104 6 0.10605737292267037 1104 7 0.1396627218475004 1104 8 0.12362537313381239 1104 9 0.10395324215711099 1104 10-11 0.2079124637681602 1104 12-13 0.11054886494638083 1104 14-15 0.24889755196367958 1104 16-17 0.28584829408497114 1104 18-19 0.29078458863221357 1104 20-21 0.3047900031545794 1104 22-23 0.3223055557078709 1104 24-25 0.35572356473608124 1104 26-27 0.3613403274140765 1104 28-29 0.3563578612728193 1104 30-31 0.30617979418579466 1104 32-33 0.2879568893845743 1104 34-35 0.25769084965206446 1104 36-37 0.3392151420996754 1104 38-39 0.3896821153147485 1104 40-41 0.5058844572862 1104 42-43 0.45718457264234047 1104 44-45 0.34194727352413423 1104 46-47 0.42584981247780007 1104 48-49 0.4619879367467874 1104 50-51 0.46825263366794445 1104 52-53 0.5586491029385172 1104 54-55 0.579240830349093 1104 56-57 0.6971513955728099 1104 58-59 0.6513149770498501 1104 60-61 0.7830029634668776 1104 62-63 0.7393137142280395 1104 64-65 0.7549140634777487 1104 66-67 0.817119798949328 1104 68-69 0.8961340441763284 1104 70-71 0.9682140333595299 1104 72-73 1.0921912120961608 1104 74-75 1.099212672994529 1104 76-77 1.1343121903547058 1104 78-79 1.291426219408848 1104 80-81 1.4674921368587341 1104 82-83 1.4305010408301975 1104 84-85 1.7333394305627898 1104 86-87 1.7576381028945818 1104 88-89 1.8842316389538674 1104 90-91 1.8900506806155057 1104 92-93 2.0898963466890983 1104 94-95 2.256633641503786 1104 96-97 2.4497051115393838 1104 98-99 2.9465207373424036 1104 100-101 3.21304939109827 1105 1 0.08536705069627715 1105 2 0.1680142102077582 1105 3 0.16548871238484963 1105 4 0.11069672669375308 1105 5 0.1281435868504488 1105 6 0.0734312729536768 1105 7 0.05308526632680355 1105 8 0.08772005298700236 1105 9 0.15087093005637087 1105 10-11 0.1287324601181652 1105 12-13 0.10112592753154104 1105 14-15 0.1445049182600755 1105 16-17 0.16605290600646327 1105 18-19 0.2168481032918308 1105 20-21 0.21512205190652622 1105 22-23 0.16658882943788456 1105 24-25 0.19208132252563104 1105 26-27 0.1596156774500983 1105 28-29 0.10651940818777916 1105 30-31 0.2193953187228601 1105 32-33 0.14043464555866336 1105 34-35 0.20862689559697856 1105 36-37 0.2032967949553992 1105 38-39 0.22483973439258875 1105 40-41 0.2089727666643313 1105 42-43 0.20128302394794417 1105 44-45 0.274628214419522 1105 46-47 0.25386004337753576 1105 48-49 0.356997625006791 1105 50-51 0.3516900058462298 1105 52-53 0.37095246435934826 1105 54-55 0.39777073216683334 1105 56-57 0.43342977673911065 1105 58-59 0.4449457773660157 1105 60-61 0.5131626033994507 1105 62-63 0.6735653344798394 1105 64-65 0.699279978171873 1105 66-67 0.888822715994678 1105 68-69 0.9116777725272982 1105 70-71 1.0669028007778252 1105 72-73 1.0094583150407175 1105 74-75 0.8309226470893272 1105 76-77 0.9080689243862352 1105 78-79 1.1420945438545687 1105 80-81 1.32896474630601 1105 82-83 1.3275606592333027 1105 84-85 1.4383368479377694 1105 86-87 1.7431704683027718 1105 88-89 1.8011310860200744 1105 90-91 1.7441850518169097 1105 92-93 1.8515223346076972 1105 94-95 1.7526700484696143 1105 96-97 2.1058771754014316 1105 98-99 2.431607861840721 1105 100-101 2.7259880236157805 1106 1 0.028953402727793787 1106 2 -0.021657540165996636 1106 3 0.07126570470028071 1106 4 0.07326377026597441 1106 5 -0.001333773528621407 1106 6 0.014311042164599996 1106 7 -0.00612507448451538 1106 8 -0.0700224641887317 1106 9 -0.04473077588747998 1106 10-11 0.07895927927764745 1106 12-13 0.03283953818791474 1106 14-15 -0.0038903997783279465 1106 16-17 0.06704593635429745 1106 18-19 -0.035975779205891456 1106 20-21 0.011180394863778531 1106 22-23 0.030869634347155284 1106 24-25 0.0837485623258587 1106 26-27 -0.007036766553007112 1106 28-29 -0.042176525239419504 1106 30-31 0.08755025783369774 1106 32-33 0.11429930776468211 1106 34-35 -0.09334700621323577 1106 36-37 0.04770737023180516 1106 38-39 0.06398536307932545 1106 40-41 0.15207917839522622 1106 42-43 0.04108254705109715 1106 44-45 0.19883322844550833 1106 46-47 -0.1744369204667109 1106 48-49 0.06532766997079165 1106 50-51 0.1136450621299332 1106 52-53 -0.004677653095484402 1106 54-55 -0.007527438763823113 1106 56-57 0.192780071543595 1106 58-59 0.21818293665054966 1106 60-61 0.31190306221760267 1106 62-63 0.3278022137370229 1106 64-65 0.3447142719278631 1106 66-67 0.4293465655365978 1106 68-69 0.05782019082070633 1106 70-71 0.2480797421005363 1106 72-73 0.4031633243672559 1106 74-75 0.5688151944683248 1106 76-77 0.5935090096506102 1106 78-79 0.8047950517080906 1106 80-81 0.9050480395945897 1106 82-83 0.9252918410456417 1106 84-85 0.9389212949777672 1106 86-87 1.0944567814109298 1106 88-89 1.396493821378428 1106 90-91 1.2335917534066816 1106 92-93 1.2673004950712716 1106 94-95 1.8069153867305552 1106 96-97 1.8391750813728187 1106 98-99 2.102952292929455 1106 100-101 2.471208947580674 1107 1 -0.006335727201523866 1107 2 -0.029143664408579184 1107 3 0.03657187355359781 1107 4 -0.011417261516783128 1107 5 -0.008654422900129077 1107 6 -0.07822549836904358 1107 7 -0.10291144393210772 1107 8 -0.10777185924458621 1107 9 -0.06004453634059814 1107 10-11 -0.02576643658820643 1107 12-13 0.035936470814853294 1107 14-15 0.0659036148136849 1107 16-17 0.05104013006405239 1107 18-19 0.013284241522477203 1107 20-21 -0.10648412097185656 1107 22-23 -0.04928929781181779 1107 24-25 -0.011590268743809418 1107 26-27 -0.04117043298764855 1107 28-29 0.021297464179291126 1107 30-31 0.0373895873083967 1107 32-33 0.031225274630095612 1107 34-35 -0.014420040337299156 1107 36-37 -0.01734523185320569 1107 38-39 0.09692051027933957 1107 40-41 0.15547023967154416 1107 42-43 0.22588453098917682 1107 44-45 0.11443767015485662 1107 46-47 0.2119422825106554 1107 48-49 0.25303250563649726 1107 50-51 0.3321624776999812 1107 52-53 0.18548994155563037 1107 54-55 0.24789219333331403 1107 56-57 0.36480308252611593 1107 58-59 0.5090786647436545 1107 60-61 0.5669549234798339 1107 62-63 0.5676378684373233 1107 64-65 0.4791191821892866 1107 66-67 0.5481444668810198 1107 68-69 0.7358619101456938 1107 70-71 0.8349285240338418 1107 72-73 0.5350129828186141 1107 74-75 0.6796102293420105 1107 76-77 0.6528247094457456 1107 78-79 0.794857977635349 1107 80-81 0.8767437682744834 1107 82-83 1.119159808822051 1107 84-85 1.269975705843951 1107 86-87 1.2723717540261568 1107 88-89 1.3940201334232505 1107 90-91 1.4794615196954304 1107 92-93 1.5614657454950418 1107 94-95 1.604981399174914 1107 96-97 1.7654754637400636 1107 98-99 1.881153032431957 1107 100-101 2.037087897137976 1108 1 -0.08100485986071249 1108 2 -0.07764125045810744 1108 3 0.0318064230696109 1108 4 -0.026523067007623524 1108 5 -0.029618561924465325 1108 6 -0.06658329146586084 1108 7 -0.07407134112904146 1108 8 -0.06821910061561454 1108 9 -0.09949641345730953 1108 10-11 -0.031635578855159 1108 12-13 -0.09690046142640085 1108 14-15 0.012047298910424331 1108 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2316 66-67 -1.4017781009477872 2316 68-69 -1.412920001699117 2316 70-71 -1.6620044499667443 2316 72-73 -1.54158292093053 2316 74-75 -1.491936028815072 2316 76-77 -1.6462724492385874 2316 78-79 -1.8028454019674136 2316 80-81 -2.1668357696957656 2316 82-83 -2.2164789264880866 2316 84-85 -2.2955173145803407 2316 86-87 -2.4440292698585537 2316 88-89 -2.5950275754745036 2316 90-91 -2.8595710587780694 2316 92-93 -3.1725620446441916 2316 94-95 -3.1372566931811967 2316 96-97 -3.437939384012271 2316 98-99 -4.064155290013407 2316 100-101 -4.375100460623575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 23.0 5 40.0 6 88.0 7 251.0 8 562.0 9 1048.0 10 1890.0 11 2984.0 12 4539.0 13 6888.0 14 9453.0 15 12421.0 16 15403.0 17 21027.0 18 21284.0 19 17575.0 20 16573.0 21 17418.0 22 18834.0 23 20480.0 24 23313.0 25 27318.0 26 31816.0 27 38415.0 28 46425.0 29 56028.0 30 68635.0 31 84103.0 32 105111.0 33 136274.0 34 186775.0 35 266791.0 36 389785.0 37 494229.0 38 274696.0 39 14803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.512917616164025 18.471995906801656 13.95439535939802 24.060691117636303 2 16.16735777084276 19.48835685717961 39.43810743905933 24.9061779329183 3 20.274737830209 22.510517204815475 29.07302285591224 28.141722109063288 4 13.253250209077777 15.9638434146151 35.78363583685564 34.99927053945149 5 14.053601326265833 36.80112341357285 34.28823761002557 14.85703765013574 6 34.14491912635587 35.151945792178694 16.340141914156156 14.362993167309277 7 29.93303346682804 31.663601562483944 19.660626185373392 18.742738785314625 8 26.767689843804902 35.30407022882066 18.8761531136895 19.052086813684937 9 25.9928886813978 16.489678231737585 19.71931990413035 37.798113182734255 10-11 25.635730005075402 26.00976860689474 26.660385771615147 21.69411561641471 12-13 26.164106020412564 23.06730968785253 27.38820246537117 23.38038182636373 14-15 22.62135244040513 24.903248873445786 25.358740478485025 27.116658207664063 16-17 22.71982400887353 28.02033202174245 26.340414855600685 22.919429113783334 18-19 22.689243641878022 26.65471447270276 28.24099321704431 22.415048668374908 20-21 22.86677995565702 25.819533515116273 28.784122828827456 22.52956370039925 22-23 22.66937354750021 25.774841213904548 28.601552209854496 22.954233028740745 24-25 22.647240919661254 25.935147644184326 28.715875846676 22.70173558947842 26-27 22.360358612491424 26.240812620410626 28.54437297277523 22.85445579432272 28-29 22.30668006960101 26.782272454915102 28.38026826142955 22.53077921405434 30-31 22.495364840431947 26.183889514809806 28.415279885965727 22.905465758792523 32-33 22.27032949555106 26.172751780100402 28.5041485159888 23.052770208359746 34-35 22.947674656377888 26.3572099863478 28.218907030851337 22.476208326422974 36-37 22.958228007556535 25.938797649360872 28.253988542720055 22.848985800362538 38-39 22.478708960877267 26.238402914135833 28.400184805274186 22.882703319712714 40-41 22.94951070239599 26.119103590840574 28.285499715756462 22.64588599100697 42-43 22.61300831855657 26.146915130006732 28.065818451757057 23.174258099679637 44-45 22.65304851697018 26.357268256875603 28.28439631881694 22.705286907337275 46-47 23.011956392640485 26.623963992324697 27.66820137108298 22.695878243951835 48-49 22.929976355150693 26.50795761064349 27.673987521881298 22.88807851232452 50-51 22.888848705772602 26.773236400697815 27.718966590706877 22.6189483028227 52-53 23.139289978033993 26.599974109287572 27.571101855295577 22.68963405738286 54-55 22.68694224521792 26.689440904448887 27.685000441785967 22.938616408547222 56-57 22.83067679555173 26.865641586237647 27.52618352405473 22.77749809415589 58-59 22.87442388027806 26.550411888357605 27.479004892522724 23.096159338841616 60-61 22.604687862803882 26.777202200299595 27.722418685697026 22.8956912511995 62-63 22.60388648360974 26.750284078650232 27.879632845039975 22.76619659270005 64-65 22.338177910290735 26.794606512541584 27.753158769656906 23.11405680751077 66-67 22.374884287679578 26.83399789791425 27.55688525550205 23.23423255890412 68-69 22.713341023852802 26.457887008542723 27.74363117726182 23.08514079034266 70-71 22.600763159571034 26.609302985034756 27.45132648804815 23.33860736734606 72-73 22.57772864478559 26.62800183289805 27.48496386601762 23.309305656298736 74-75 22.49720030986662 27.06082879047222 27.177213707282892 23.26475719237827 76-77 22.641407468443127 26.7270646302046 27.385921616895537 23.245606284456738 78-79 22.639332101812144 26.821606826928807 27.450956620727773 23.088104450531276 80-81 22.75362676201044 26.825319524925 27.27984629926437 23.14120741380019 82-83 22.610658474689462 26.932878527909914 27.338217012051658 23.118245985348963 84-85 22.602542539189688 26.979786885363804 27.200175900230043 23.21749467521646 86-87 22.678558145517805 26.977770362099267 27.114540314464037 23.229131177918884 88-89 22.573792670377397 27.179998882178307 26.876645910252066 23.36956253719223 90-91 22.649717810169932 26.974902417698488 27.00956866516453 23.36581110696705 92-93 22.62223749186289 26.734293731629855 27.20306119765125 23.440407578856004 94-95 22.448978333784368 26.581835524489435 27.31552914976321 23.653656991962986 96-97 22.6298190461885 26.373890113448788 27.27441596300991 23.721874877352796 98-99 22.37026525483998 26.624772967920336 26.999818970234475 24.005142807005214 100-101 22.70812044063728 26.10082776173498 27.10411616404983 24.08693563357791 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 488.0 1 331.0 2 270.5 3 392.0 4 347.5 5 340.5 6 646.5 7 1257.0 8 1963.5 9 2306.5 10 2038.5 11 1717.0 12 1753.5 13 1895.5 14 2050.5 15 2181.5 16 2327.5 17 2609.5 18 2868.0 19 3186.5 20 3693.5 21 4390.0 22 5375.5 23 6711.0 24 8423.0 25 10674.5 26 13565.0 27 16952.0 28 20084.0 29 23192.0 30 26615.0 31 30987.0 32 37748.0 33 41158.0 34 41586.5 35 44427.5 36 48897.0 37 53406.0 38 56975.5 39 60097.5 40 63884.5 41 68609.5 42 71344.5 43 73969.5 44 78113.5 45 83130.0 46 86023.0 47 89000.5 48 92820.5 49 92673.5 50 91253.0 51 89696.5 52 88856.0 53 90553.0 54 92185.0 55 87331.0 56 79694.5 57 73321.0 58 67489.0 59 59737.0 60 48605.0 61 40214.5 62 34149.0 63 26594.0 64 19394.0 65 14495.5 66 10873.0 67 8533.0 68 6672.5 69 4909.5 70 3776.5 71 2748.5 72 1965.5 73 1524.5 74 1154.5 75 820.5 76 577.5 77 364.0 78 223.0 79 135.0 80 77.5 81 55.0 82 31.5 83 16.5 84 12.5 85 6.0 86 2.5 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009945321281138205 3 0.006123358970618151 4 0.0 5 2.8767458251226214E-4 6 1.232891067909695E-4 7 0.0 8 8.219273786064632E-5 9 4.109636893032316E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.054818446516158E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016233065727477647 26-27 0.01989064256227641 28-29 2.8767458251226214E-4 30-31 6.164455339548475E-5 32-33 0.0035137395435426307 34-35 0.014938530106172469 36-37 0.007376798222993006 38-39 0.01947967887297318 40-41 0.02186326827093192 42-43 0.01771253500896928 44-45 0.009390520300578842 46-47 0.0018904329707948653 48-49 2.260300291167774E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.082227669774237E-4 68-69 2.46578213581939E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0548184465161583E-4 82-83 0.0012328910679096949 84-85 0.00441785966000974 86-87 9.041201164671096E-4 88-89 4.109636893032316E-5 90-91 0.004212377815358124 92-93 3.6986732037290845E-4 94-95 2.054818446516158E-5 96-97 1.8493366018645422E-4 98-99 1.4383729125613107E-4 100-101 0.0023219448445632586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2433305.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.52029614511387 #Duplication Level Percentage of deduplicated Percentage of total 1 77.82183691228339 44.76335105751294 2 12.919421183357906 14.862578649804083 3 4.056406859169924 6.999771714735756 4 1.7671084654934042 4.065784090228733 5 0.8984948225682833 2.5840844139489594 6 0.5440068073743249 1.8774859599077451 7 0.3619358461122018 1.4573059937734345 8 0.2549380035400998 1.1731287569816502 9 0.18687330084995893 0.9674106845854137 >10 1.0761150093855238 11.346804505517648 >50 0.07493801010834464 2.9466258720864094 >100 0.03304698388405426 3.4901057236990503 >500 0.0030127562743181343 1.2107518366743013 >1k 0.0017215750138941286 1.704311456025978 >5k 1.4346458449117738E-4 0.5504992845179537 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7176 0.294907543443999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6166 0.2534002108243726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3773 0.15505659997410928 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2927 0.1202890718590559 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2794 0.11482325479132292 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2524 0.10372723518013566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.054818446516158E-4 2 0.0 0.0 0.0 0.0 2.054818446516158E-4 3 0.0 0.0 0.0 0.0 2.054818446516158E-4 4 0.0 0.0 0.0 0.0 2.054818446516158E-4 5 0.0 0.0 0.0 0.0 2.054818446516158E-4 6 0.0 0.0 0.0 0.0 2.8767458251226214E-4 7 0.0 0.0 0.0 0.0 2.8767458251226214E-4 8 0.0 0.0 0.0 0.0 3.287709514425853E-4 9 0.0 0.0 0.0 0.0 3.287709514425853E-4 10-11 0.0 0.0 0.0 0.0 3.287709514425853E-4 12-13 0.0 0.0 0.0 0.0 7.602828252109785E-4 14-15 0.0 0.0 0.0 0.0 8.835719320019479E-4 16-17 0.0 0.0 0.0 0.0 0.001027409223258079 18-19 0.0 0.0 0.0 0.0 0.0011096019611187254 20-21 0.0 0.0 0.0 6.164455339548475E-5 0.0011917946989793717 22-23 0.0 0.0 0.0 2.260300291167774E-4 0.0012328910679096949 24-25 0.0 0.0 0.0 4.520600582335548E-4 0.0012328910679096949 26-27 0.0 0.0 0.0 7.191864562806553E-4 0.0013356319902355029 28-29 0.0 0.0 0.0 0.0033082576988910147 0.0013561801747006643 30-31 0.0 0.0 0.0 0.011260405086908546 0.0013561801747006643 32-33 4.109636893032316E-5 0.0 0.0 0.02767840447457265 0.0013561801747006643 34-35 4.109636893032316E-5 0.0 0.0 0.05356911690067624 0.0013561801747006643 36-37 4.109636893032316E-5 0.0 0.0 0.09875457453956656 0.0013561801747006643 38-39 4.109636893032316E-5 0.0 0.0 0.16678961330371656 0.0013972765436309875 40-41 4.109636893032316E-5 0.0 0.0 0.23872880711624722 0.0013972765436309875 42-43 4.109636893032316E-5 0.0 0.0 0.3114488319384541 0.0013972765436309875 44-45 4.109636893032316E-5 0.0 0.0 0.39871697136199535 0.0014794692814916338 46-47 4.109636893032316E-5 0.0 0.0 0.5003893880956148 0.0016233065727477647 48-49 4.109636893032316E-5 0.0 0.0 0.6121715115860938 0.0017465956795387344 50-51 4.109636893032316E-5 0.0 0.0 0.7283098501831871 0.0017671438640038959 52-53 4.109636893032316E-5 0.0 0.0 0.839064564450408 0.0019315293397251885 54-55 4.109636893032316E-5 0.0 0.0 0.9586755462221135 0.001972625708655512 56-57 4.109636893032316E-5 0.0 0.0 1.097067568594977 0.002013722077585835 58-59 4.109636893032316E-5 0.0 0.0 1.243493931093718 0.002013722077585835 60-61 4.109636893032316E-5 0.0 0.0 1.3982217601163849 0.002013722077585835 62-63 4.109636893032316E-5 0.0 0.0 1.546250881003409 0.002219203922237451 64-65 4.109636893032316E-5 0.0 0.0 1.7000745899096086 0.002301396660098097 66-67 4.109636893032316E-5 0.0 0.0 1.8744875796499 0.002301396660098097 68-69 4.109636893032316E-5 0.0 0.0 2.070825482214519 0.002301396660098097 70-71 4.109636893032316E-5 0.0 0.0 2.26868395042956 0.0023219448445632586 72-73 4.109636893032316E-5 0.0 0.0 2.4697684836056313 0.0025274266892148746 74-75 4.109636893032316E-5 0.0 0.0 2.6709352095195626 0.0027123603494013286 76-77 4.109636893032316E-5 0.0 0.0 2.889054187617253 0.0027534567183316516 78-79 4.109636893032316E-5 0.0 0.0 3.1340296428109093 0.002815101271727137 80-81 4.109636893032316E-5 0.0 0.0 3.388888774732308 0.0029178421940529446 82-83 4.109636893032316E-5 0.0 0.0 3.648371248158369 0.0032055167765652065 84-85 4.109636893032316E-5 0.0 0.0 3.9114291056813677 0.0032055167765652065 86-87 4.109636893032316E-5 0.0 0.0 4.177281516291628 0.0032466131454955294 88-89 4.109636893032316E-5 0.0 0.0 4.463558822260259 0.0033082576988910147 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5560 0.0 33.660587 1 GTATCAA 7895 0.0 31.04544 1 ATCAACG 9585 0.0 25.371283 3 TCAACGC 9665 0.0 25.209383 4 GTGTACG 245 1.0913936E-11 25.204468 1 TATCAAC 9735 0.0 24.982407 2 CAACGCA 9755 0.0 24.928114 5 AACGCAG 10185 0.0 23.922308 6 TACATGG 6435 0.0 23.325922 2 GTACATG 6700 0.0 23.112293 1 CGCAGAG 11575 0.0 21.049564 8 ACGCAGA 11630 0.0 20.990856 7 ACATGGG 6870 0.0 20.740973 3 GAGTACT 7485 0.0 20.59063 12-13 AGAGTAC 10120 0.0 19.687845 10-11 GTACTTT 7880 0.0 19.287258 14-15 GTGTAGC 1585 0.0 19.180105 1 GCAGAGT 12950 0.0 18.704544 9 AGTACTT 7950 0.0 18.699238 12-13 AGGCGAC 820 0.0 18.534584 5 >>END_MODULE