FastQCFastQC Report
Thu 26 May 2016
SRR937913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493212
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77640.3114055282904141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.2526459843767798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.15975376341843373No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30160.12096845354506555No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29200.11711799879031548No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26060.10452380302998703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.033.739371
GTATCAA83600.033.226071
ATCAACG98250.027.858933
TCAACGC99400.027.4888114
CAACGCA100050.027.3102255
TATCAAC101050.027.1810362
GTACATG68300.026.4838311
AACGCAG104500.026.1017786
TACATGG66300.025.80262
ACATGGG69950.023.980683
ACGCAGA117650.023.0733437
CGCAGAG118800.022.6494488
GAGTACT73050.021.37592112-13
GCAGAGT130700.020.2958649
AGAGTAC99700.020.21693410-11
GGACCGA5450.020.0542226
CATGGGG48650.019.9260794
GTACTTT79050.019.74017714-15
GAGTACA49850.019.0524221
TAACCCG1759.910965E-519.0079155
Produced by FastQC (version 0.11.5)