FastQCFastQC Report
Thu 26 May 2016
SRR937913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493212
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76220.30571006396567957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65600.26311440824125665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40000.16043561478125407No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30150.12092834464137024No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28830.11563396935358886No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26110.10472434754846358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60200.036.3805271
GTATCAA88050.030.5927331
ATCAACG101850.025.9308853
TCAACGC103700.025.4662384
TATCAAC105400.025.2413162
CAACGCA105100.025.1270125
TACATGG66750.024.412232
GTACATG69550.024.3857231
AACGCAG109700.024.0733726
TATTGCG1503.0363908E-522.1653985
ACATGGG68150.022.0951523
GAGTACT78500.021.93345812-13
CGCAGAG124000.021.143958
ACGCAGA125500.020.9669287
GGACCGA5000.020.8988046
GTACTTT82750.020.83566514-15
GTGTAGC18850.020.4144761
CATGGGG47100.019.5636374
ATTGCGC1951.0339503E-519.4860656
CGTATAG1250.00602738619.0031051
Produced by FastQC (version 0.11.5)