FastQCFastQC Report
Thu 26 May 2016
SRR937912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414249
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70050.29015234136992496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58560.2425599016505754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.14236311167572194No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29330.1214870545664511No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28970.11999590763007462No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25270.10467023078398292No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24530.1016050954147646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83900.032.175281
GGTATCA57550.031.9332581
ATCAACG98600.027.0621953
TCAACGC99550.026.7561654
CAACGCA100200.026.6300645
TATCAAC100650.026.6055182
GTACATG62500.026.0523781
AACGCAG103650.025.7388696
TACATGG61750.024.9565832
ACGCAGA118050.022.6741037
CGCAGAG119250.022.3648058
ACATGGG66300.021.6655883
GAGTACT72600.021.27954512-13
GTATAGG5950.020.8045181
AGAGTAC98150.020.70541410-11
GCAGAGT127850.020.6368719
GTACTTT74800.020.52572314-15
CATGGGG43050.020.1082974
GTGTAGC18750.019.80591
AGTACTT75650.019.2905712-13
Produced by FastQC (version 0.11.5)