##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937912_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2414249 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10948166489869 33.0 31.0 34.0 30.0 34.0 2 32.23148813564798 34.0 31.0 34.0 30.0 34.0 3 32.26908989089361 34.0 31.0 34.0 30.0 34.0 4 35.76916796900402 37.0 35.0 37.0 35.0 37.0 5 35.76929223124873 37.0 35.0 37.0 35.0 37.0 6 35.79425858724597 37.0 36.0 37.0 35.0 37.0 7 35.73177828798935 37.0 36.0 37.0 35.0 37.0 8 35.72462741001446 37.0 36.0 37.0 35.0 37.0 9 37.39154660517619 39.0 37.0 39.0 35.0 39.0 10-11 37.303475532142706 39.0 37.0 39.0 34.0 39.0 12-13 37.1620694468549 39.0 37.0 39.0 34.0 39.0 14-15 38.3959359618664 40.0 38.0 41.0 34.0 41.0 16-17 38.218294177609685 40.0 38.0 41.0 33.0 41.0 18-19 38.37075504639331 40.0 38.0 41.0 34.0 41.0 20-21 38.36197115541934 40.0 38.0 41.0 34.0 41.0 22-23 38.25568903621789 40.0 38.0 41.0 34.0 41.0 24-25 38.141347474929056 40.0 38.0 41.0 33.5 41.0 26-27 37.970010756968314 40.0 38.0 41.0 33.0 41.0 28-29 37.803273605995074 40.0 38.0 41.0 33.0 41.0 30-31 37.602514487942216 40.0 37.5 41.0 32.5 41.0 32-33 37.33680701534929 40.0 37.0 41.0 31.5 41.0 34-35 37.114676655142034 40.0 36.5 41.0 31.0 41.0 36-37 36.86523158961648 39.5 36.0 41.0 30.5 41.0 38-39 36.57558085350766 39.0 36.0 41.0 30.0 41.0 40-41 36.482823437019135 39.0 36.0 41.0 30.0 41.0 42-43 36.283738545609836 39.0 35.5 41.0 30.0 41.0 44-45 35.98855606857454 39.0 35.0 41.0 29.0 41.0 46-47 35.651788817143554 39.0 35.0 40.0 27.5 41.0 48-49 35.271467027634685 39.0 35.0 40.0 26.0 41.0 50-51 34.55172954405283 37.5 33.5 39.5 24.5 40.5 52-53 34.6255827381517 38.0 34.0 40.0 26.0 40.5 54-55 34.71814609843475 38.0 34.0 40.0 25.5 41.0 56-57 34.40505826035343 38.0 34.0 40.0 23.5 41.0 58-59 33.9994797554022 38.0 33.5 40.0 22.5 41.0 60-61 33.498140622611835 37.0 33.0 40.0 21.0 41.0 62-63 32.9579562837139 36.5 32.0 39.5 18.5 41.0 64-65 32.41978644290626 36.0 31.5 39.0 15.0 40.5 66-67 31.85558593997554 35.5 31.0 39.0 9.5 40.0 68-69 31.178433127651704 35.0 30.5 38.0 6.5 40.0 70-71 30.596449661985986 35.0 29.5 37.5 2.0 40.0 72-73 29.906602840055022 34.0 29.0 37.0 2.0 39.0 74-75 29.236789991421766 34.0 28.5 36.0 2.0 39.0 76-77 28.499766179876225 34.0 26.5 36.0 2.0 38.5 78-79 27.746183388705973 33.5 26.0 35.0 2.0 37.0 80-81 27.034742480995128 33.0 25.0 35.0 2.0 37.0 82-83 26.298605901876734 32.0 23.5 35.0 2.0 36.0 84-85 25.581207447947584 32.0 20.5 35.0 2.0 36.0 86-87 24.853194720180063 31.0 17.5 34.0 2.0 35.0 88-89 23.985049595132896 31.0 9.0 34.0 2.0 35.0 90-91 23.10096845851443 30.0 2.0 34.0 2.0 35.0 92-93 22.17636954597475 29.5 2.0 34.0 2.0 35.0 94-95 21.10581799971751 29.0 2.0 34.0 2.0 35.0 96-97 19.85210079821924 28.0 2.0 34.0 2.0 35.0 98-99 18.294735754265613 25.0 2.0 33.0 2.0 35.0 100-101 14.250880294451814 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17858209947891623 1101 2 0.08082923120849017 1101 3 0.016658778296466892 1101 4 -0.12698806489427028 1101 5 0.01777918362325437 1101 6 0.07499134503078153 1101 7 0.07737976644178701 1101 8 0.1439878884133492 1101 9 0.11653673918822705 1101 10-11 0.12822456520696335 1101 12-13 0.09710815806516138 1101 14-15 0.13614902985570865 1101 16-17 0.023769302954576688 1101 18-19 0.22960337201425318 1101 20-21 0.2043490012659035 1101 22-23 0.15107065771341155 1101 24-25 0.2096528642051041 1101 26-27 0.14995720867660367 1101 28-29 0.10916393212269071 1101 30-31 0.03587742435976793 1101 32-33 -0.11940228230592709 1101 34-35 -0.009886308629596385 1101 36-37 -0.10770414259365424 1101 38-39 0.0050022077528808495 1101 40-41 0.013006966344235593 1101 42-43 0.0424008368760056 1101 44-45 -0.021110967593379826 1101 46-47 0.02978697062512481 1101 48-49 0.12515143544458596 1101 50-51 -0.12301214214217282 1101 52-53 -0.07000034672697097 1101 54-55 -0.127329382769112 1101 56-57 -0.10377867885716796 1101 58-59 -0.20306779011732345 1101 60-61 -0.0734356483694043 1101 62-63 -0.33575270842514016 1101 64-65 -0.4519587015434112 1101 66-67 -0.6993084025490539 1101 68-69 -0.6855279127705955 1101 70-71 -0.5765017245484003 1101 72-73 -0.6871390223417606 1101 74-75 -0.7717950316064872 1101 76-77 -1.071538924746438 1101 78-79 -1.2659475105812064 1101 80-81 -1.2787573773774703 1101 82-83 -1.2475166328389413 1101 84-85 -1.3682298886351134 1101 86-87 -1.3151164619316944 1101 88-89 -1.2350467875673878 1101 90-91 -1.3296995928095008 1101 92-93 -1.46090884747953 1101 94-95 -1.4555692810421412 1101 96-97 -1.703339557341689 1101 98-99 -1.9097617200288681 1101 100-101 -2.162141955466007 1102 1 0.15660630869243874 1102 2 0.1822401214412679 1102 3 0.23618793709161423 1102 4 0.10479750686214118 1102 5 0.047215596958835704 1102 6 0.14477625851920806 1102 7 0.11109265308679284 1102 8 0.16475735869288854 1102 9 0.2156563577482089 1102 10-11 0.26444652666785373 1102 12-13 0.3109090431539201 1102 14-15 0.2586625889095515 1102 16-17 0.2413469108026689 1102 18-19 0.2874271667949557 1102 20-21 0.22649625933021156 1102 22-23 0.353132650979866 1102 24-25 0.2464204961739469 1102 26-27 0.23095923924996242 1102 28-29 0.15416842108687234 1102 30-31 0.12686605326242528 1102 32-33 0.1843690147523418 1102 34-35 0.06629677305139836 1102 36-37 0.14547806889563475 1102 38-39 0.027915019005213537 1102 40-41 0.06609385941954571 1102 42-43 0.20917053514842365 1102 44-45 0.13713593734663476 1102 46-47 0.019917015416460515 1102 48-49 0.08135685891488009 1102 50-51 0.12141767622539845 1102 52-53 -0.03287163151597383 1102 54-55 -0.07372430688396747 1102 56-57 -0.06607695667915436 1102 58-59 -0.13268671118169095 1102 60-61 -0.05457727258988143 1102 62-63 0.00458185882983031 1102 64-65 0.027288842570655447 1102 66-67 -0.08585302307986709 1102 68-69 -0.1864226808256575 1102 70-71 -0.41111406765914893 1102 72-73 -0.32240392416209573 1102 74-75 -0.2833050798347294 1102 76-77 -0.5396685078805596 1102 78-79 -0.59176681507439 1102 80-81 -0.5898735991082908 1102 82-83 -0.7506027602665384 1102 84-85 -0.7016513187175804 1102 86-87 -0.4320199973147325 1102 88-89 -0.2739695760601357 1102 90-91 -0.4477511271391599 1102 92-93 -0.5036770779769206 1102 94-95 -0.521681448621063 1102 96-97 -0.6039386341406932 1102 98-99 -0.6040199671417597 1102 100-101 -0.7067365865930082 1103 1 0.1915983297425825 1103 2 0.181876468098487 1103 3 0.14436601736451848 1103 4 0.1003199564134789 1103 5 0.13374147155336402 1103 6 0.12187378847803387 1103 7 0.16994132220894898 1103 8 0.1503815158218913 1103 9 0.13595255394523065 1103 10-11 0.14569585717764966 1103 12-13 0.254058819441191 1103 14-15 0.24327900879183062 1103 16-17 0.24760015525517787 1103 18-19 0.20450459144812783 1103 20-21 0.31978762421129403 1103 22-23 0.26762055621026803 1103 24-25 0.26261460575884854 1103 26-27 0.246531512605209 1103 28-29 0.2815970568649746 1103 30-31 0.21096061411805067 1103 32-33 0.2128133529938978 1103 34-35 0.2241116119754949 1103 36-37 0.12172469397837915 1103 38-39 0.11899232830007378 1103 40-41 0.22053886234470355 1103 42-43 0.0220821514556917 1103 44-45 0.1111174195910678 1103 46-47 0.15892158305625514 1103 48-49 0.3142070606545957 1103 50-51 0.3602555236393101 1103 52-53 0.46540595903343274 1103 54-55 0.5195244852870502 1103 56-57 0.47031721717680597 1103 58-59 0.4689381930297074 1103 60-61 0.45262548062847685 1103 62-63 0.44780359673025316 1103 64-65 0.5046258783564497 1103 66-67 0.2981731467770068 1103 68-69 0.3857476759336542 1103 70-71 0.22440915094760072 1103 72-73 0.30187350708298055 1103 74-75 0.3405617307551623 1103 76-77 0.3519067647907832 1103 78-79 0.1724193732398156 1103 80-81 0.2198077098240141 1103 82-83 0.054555129555286186 1103 84-85 -0.024248638559484448 1103 86-87 -0.03894424261044804 1103 88-89 0.2643343271707188 1103 90-91 0.10940699339928983 1103 92-93 -0.09717306652898117 1103 94-95 -0.2616509061348715 1103 96-97 -0.460744911830119 1103 98-99 -0.6684261602350929 1103 100-101 -0.7486064349517623 1104 1 0.24221297050334556 1104 2 0.28342778926389656 1104 3 0.3021930972410516 1104 4 0.1953644710346154 1104 5 0.26547977196906913 1104 6 0.23532184285902247 1104 7 0.20498744256791923 1104 8 0.31896882335637144 1104 9 0.36365063954889365 1104 10-11 0.21690185550054508 1104 12-13 0.24615858928542877 1104 14-15 0.32391202633005634 1104 16-17 0.22667382293881388 1104 18-19 0.29108329936440924 1104 20-21 0.27351739637493466 1104 22-23 0.384823360899027 1104 24-25 0.40776929183709854 1104 26-27 0.3347761735269117 1104 28-29 0.2606198761837817 1104 30-31 0.3112451865457544 1104 32-33 0.40093847565442076 1104 34-35 0.5120529668613969 1104 36-37 0.5740110862422867 1104 38-39 0.4721168265070048 1104 40-41 0.49577169433100465 1104 42-43 0.3714991794133766 1104 44-45 0.4131491239925751 1104 46-47 0.3765311746635973 1104 48-49 0.36408440012541377 1104 50-51 0.2763459088379747 1104 52-53 0.32838032075264323 1104 54-55 0.3235464125292822 1104 56-57 0.523765539180765 1104 58-59 0.47949145892204825 1104 60-61 0.34316679269470285 1104 62-63 0.376635065623546 1104 64-65 0.32290452739568565 1104 66-67 0.2561670449732283 1104 68-69 0.37993624308025886 1104 70-71 0.29731366307092344 1104 72-73 0.21289689733079697 1104 74-75 0.29156754709443433 1104 76-77 0.24966676050881276 1104 78-79 0.20728975451334364 1104 80-81 0.2779224843962602 1104 82-83 0.2150771727674794 1104 84-85 0.20761901295971796 1104 86-87 0.13166228025347593 1104 88-89 0.045399555809947145 1104 90-91 0.031795439223479605 1104 92-93 -0.07293088558017047 1104 94-95 -0.2201269717203438 1104 96-97 -0.32006265166490877 1104 98-99 -0.225241625274613 1104 100-101 -0.8208392134134659 1105 1 0.3229663396127975 1105 2 0.21063000485239058 1105 3 0.27981202982435605 1105 4 0.2815351610765404 1105 5 0.20032007359279902 1105 6 0.18734417129758896 1105 7 0.28070569701548465 1105 8 0.2269157803732682 1105 9 0.22430927672039758 1105 10-11 0.29341860182946533 1105 12-13 0.2557146088185007 1105 14-15 0.25498375687032393 1105 16-17 0.29040305179668024 1105 18-19 0.36950805735796166 1105 20-21 0.3894820573933515 1105 22-23 0.2660667822922207 1105 24-25 0.27633045346806995 1105 26-27 0.3237271337878056 1105 28-29 0.22153044164199542 1105 30-31 0.2310293219658348 1105 32-33 0.28195542042001165 1105 34-35 0.2866008763742798 1105 36-37 0.25265412456644754 1105 38-39 0.31664989640847807 1105 40-41 0.18566134629035247 1105 42-43 0.19282551282147864 1105 44-45 0.16572664370682588 1105 46-47 0.35958468354147755 1105 48-49 0.4388747474758574 1105 50-51 0.45086922945986174 1105 52-53 0.4670539010677288 1105 54-55 0.379660418706699 1105 56-57 0.2668931598388937 1105 58-59 0.4076953482806758 1105 60-61 0.3859160895386964 1105 62-63 0.32262103701415157 1105 64-65 0.36200172685041565 1105 66-67 0.3927035486917596 1105 68-69 0.3322117939576934 1105 70-71 0.13009324307540737 1105 72-73 -0.0395826982035139 1105 74-75 -0.14561473855082951 1105 76-77 -0.31538681739051455 1105 78-79 -0.3309593438939835 1105 80-81 -0.2602735537725991 1105 82-83 -0.24436237307139308 1105 84-85 -0.3743085835408948 1105 86-87 -0.22845583270012781 1105 88-89 -0.24330724107718638 1105 90-91 -0.43079407296743355 1105 92-93 -0.4435268857872323 1105 94-95 -0.7071349782083587 1105 96-97 -0.6677116085782693 1105 98-99 -0.7338908755291271 1105 100-101 -1.1721484640130893 1106 1 0.29331445913373244 1106 2 0.2998873279556449 1106 3 0.22877621957295702 1106 4 0.2838707032218082 1106 5 0.15613984968283035 1106 6 0.20309896826523044 1106 7 0.23091228120788543 1106 8 0.12948077194949548 1106 9 0.26346532524850375 1106 10-11 0.28019830945331137 1106 12-13 0.29562017887031544 1106 14-15 0.369556834471652 1106 16-17 0.038635055757993086 1106 18-19 0.3252449407312952 1106 20-21 0.5201118128897235 1106 22-23 0.5381465824729545 1106 24-25 0.29826454379523426 1106 26-27 0.3389225045483997 1106 28-29 0.25176984417679904 1106 30-31 0.29750848371323 1106 32-33 0.34562439951451296 1106 34-35 0.22159196441092632 1106 36-37 0.11283901916218753 1106 38-39 0.04380991422244307 1106 40-41 0.03060354555766054 1106 42-43 0.0076506560156559544 1106 44-45 0.14864048210787217 1106 46-47 0.10051724653047955 1106 48-49 0.03828911840282956 1106 50-51 0.028023319249008694 1106 52-53 0.13944923157752953 1106 54-55 -0.06558415964136088 1106 56-57 -0.16521621072443082 1106 58-59 -0.17025309683329226 1106 60-61 0.12887842814946993 1106 62-63 0.06738718888915685 1106 64-65 -0.16097210913957127 1106 66-67 -0.2425253577520543 1106 68-69 -0.14771422030742443 1106 70-71 -0.011005603500336747 1106 72-73 -0.28799134734116194 1106 74-75 -0.23869668377986386 1106 76-77 -0.3573435836579648 1106 78-79 -0.42197176086427746 1106 80-81 -0.5028913695295891 1106 82-83 -0.3806216598951373 1106 84-85 -0.20118086798246892 1106 86-87 -0.11508778804167363 1106 88-89 -0.5832728884224814 1106 90-91 -0.6042784663212721 1106 92-93 -0.6218744513616485 1106 94-95 -0.8203627428847042 1106 96-97 -0.9637568687986295 1106 98-99 -1.01061117338498 1106 100-101 -1.3192668959857645 1107 1 -0.09648817611149951 1107 2 -0.021402534902527748 1107 3 -0.08213456950711873 1107 4 -3.8559009244210074E-4 1107 5 -0.008646754598679252 1107 6 0.012235763097869778 1107 7 -0.009318861883421903 1107 8 -0.034042488365862766 1107 9 0.005612753644150814 1107 10-11 0.050793268114595946 1107 12-13 -0.07573068709893249 1107 14-15 0.007850088538098987 1107 16-17 -0.02914257712810553 1107 18-19 0.04697369533903384 1107 20-21 0.08375167385315763 1107 22-23 0.19842863455519222 1107 24-25 0.17049079697820702 1107 26-27 0.13538647890479893 1107 28-29 0.04884970103868369 1107 30-31 -0.03848714130116093 1107 32-33 -0.03474235104072676 1107 34-35 -0.016865277312227533 1107 36-37 -0.033750119311910964 1107 38-39 0.0018197490236318004 1107 40-41 0.14263555472967937 1107 42-43 0.07698903232237342 1107 44-45 0.11077610740462518 1107 46-47 -0.053619262233979725 1107 48-49 -0.024882166593151567 1107 50-51 -0.11818482841864153 1107 52-53 -0.26031535659946314 1107 54-55 -0.2741500626917386 1107 56-57 -0.22289563677507118 1107 58-59 -0.4362300976944695 1107 60-61 -0.33297177911993003 1107 62-63 -0.2558172874463196 1107 64-65 -0.2586540837976443 1107 66-67 -0.2898812518340108 1107 68-69 -0.25756198745238734 1107 70-71 -0.2264901769949681 1107 72-73 -0.40898304913476835 1107 74-75 -0.45338593200011346 1107 76-77 -0.6685468495906619 1107 78-79 -0.57783610287402 1107 80-81 -0.6080562562577363 1107 82-83 -0.7076437532752209 1107 84-85 -0.6068111542066887 1107 86-87 -0.6318969872513804 1107 88-89 -0.4705912815663531 1107 90-91 -0.7578959656692597 1107 92-93 -0.8769131523000269 1107 94-95 -1.0829215076051426 1107 96-97 -1.0175870059701815 1107 98-99 -1.1493794120891856 1107 100-101 -0.9925788893757339 1108 1 0.007869842733050803 1108 2 -0.015990804358658295 1108 3 0.07374781393586005 1108 4 0.11757204480904448 1108 5 0.09715393147485685 1108 6 0.1517147205518654 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0.2327957188640788 2316 90-91 0.4382248943707374 2316 92-93 0.4334359058782873 2316 94-95 0.49907583368480246 2316 96-97 0.43499784289620536 2316 98-99 0.14354462727612116 2316 100-101 0.09372406887842111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7030.0 3 2090.0 4 1853.0 5 2151.0 6 2443.0 7 3037.0 8 3849.0 9 5040.0 10 6074.0 11 7769.0 12 10055.0 13 12182.0 14 14593.0 15 17788.0 16 20704.0 17 25617.0 18 25278.0 19 22891.0 20 23918.0 21 25580.0 22 28505.0 23 31494.0 24 35626.0 25 40040.0 26 45238.0 27 51962.0 28 60103.0 29 70087.0 30 82450.0 31 99091.0 32 120654.0 33 150596.0 34 194182.0 35 257792.0 36 333252.0 37 364784.0 38 195387.0 39 13064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32360772412947 17.91330866361676 13.558126135782441 24.204957476471336 2 15.885968147666194 19.060385162100445 40.00949022175623 25.04415646847713 3 19.91945533620843 22.396236934722978 28.924187791526386 28.76011993754221 4 12.928079138081053 15.50416435451883 36.06647870874734 35.50127779865277 5 14.088663531435042 36.66531928067848 34.203669980627524 15.042347207258953 6 34.06595778147516 34.95472732849114 16.557176954162067 14.422137935871634 7 29.993428171239287 31.584980715997425 19.759256985057387 18.6623341277059 8 26.88022647812832 34.98767924164644 18.97832002470369 19.153774255521544 9 26.222946949957432 16.497886848196035 19.703134415199596 37.576031786646936 10-11 25.68163339092477 25.890044725615585 26.705384363739036 21.72293751972061 12-13 26.280084136956315 22.998651576883454 27.29126665279733 23.429997633362902 14-15 22.75648058241353 24.80402815009791 25.35939406030777 27.080097207180785 16-17 22.790586169259903 27.929752348055324 26.30543663802069 22.97422484466408 18-19 22.762782404491638 26.563975946664094 28.204728338196688 22.468513310647584 20-21 23.00241703934973 25.795212167527477 28.648583631531633 22.55378716159116 22-23 22.7568107765549 25.730859210127132 28.49596622075619 23.016363792561776 24-25 22.71576517098372 25.86585634652767 28.606290593664248 22.812087888824365 26-27 22.394858722728188 26.193799687640652 28.466286553407016 22.945055036224144 28-29 22.39421198661242 26.722800774721193 28.30766136223193 22.57532587643446 30-31 22.567927862037827 26.152001437883378 28.265851700241118 23.01421899983768 32-33 22.2991310074491 26.174533384094378 28.392591873754302 23.13374373470222 34-35 23.015169043931706 26.294080492925005 28.092716135089212 22.598034328054077 36-37 23.008048500355592 25.898266242459904 28.157480230089817 22.93620502709469 38-39 22.56829148364052 26.245881458528842 28.23465445071851 22.951172607112124 40-41 22.990881456447106 26.146759998267594 28.138072690974358 22.724285854310942 42-43 22.652725299515517 26.10216093815763 28.010816606545625 23.234297155781224 44-45 22.70452760840424 26.375406341096546 28.17391502524546 22.74615102525375 46-47 23.096242649883532 26.643402953183454 27.51468365645735 22.74567074047567 48-49 22.98252840279277 26.52591069814701 27.581590821599754 22.909970077460468 50-51 22.898792774050236 26.796582634678423 27.60995785277065 22.694666738500686 52-53 23.134508307669872 26.6265967244956 27.469917505416152 22.768977462418373 54-55 22.714402195591205 26.681033187699633 27.657880641147443 22.94668397556172 56-57 22.88356497397163 26.86253180652353 27.419786863406014 22.834116356098825 58-59 22.89672955370153 26.52345375308122 27.402275338126252 23.177541355090998 60-61 22.670967215291864 26.714437342010672 27.698490325941005 22.916105116756455 62-63 22.64694382171943 26.67521893340069 27.859820460417517 22.818016784462365 64-65 22.420758534313883 26.736930868768443 27.71564338465714 23.12666721226053 66-67 22.47285361775656 26.72521707782481 27.566242145108056 23.235687159310572 68-69 22.832993284334503 26.36476394658554 27.74998782455337 23.05225494452659 70-71 22.7267867200059 26.49378383519486 27.425182854582896 23.35424659021634 72-73 22.709462108383953 26.59559787054696 27.430984652389085 23.26395536868 74-75 22.61288926358816 26.966185278476313 27.22161433734413 23.1993111205914 76-77 22.85738610976061 26.605299338178284 27.259166517691703 23.278148034369405 78-79 22.905818568319276 26.6890925433028 27.29444206569129 23.110646822686633 80-81 23.120113455055282 26.737611983213753 27.060837083197477 23.081437478533488 82-83 22.93776825812439 26.819225096531497 27.10975175248165 23.13325489286247 84-85 22.94061291769246 26.85981441989906 27.006575778886454 23.192996883522024 86-87 23.05800691183383 26.795070528007674 26.924064082058706 23.222858478099788 88-89 22.991365171629287 26.944121266430443 26.717650272250133 23.34686328969014 90-91 23.079765110251515 26.83654562044817 26.685919448896882 23.39776982040343 92-93 23.217331862535996 26.51827283290573 26.892770666298553 23.371624638259718 94-95 22.992750048706075 26.394962755396012 27.023416223475916 23.588870972421997 96-97 23.228669069295606 26.110165856003913 26.97216943300582 23.688995641694664 98-99 23.019571218184883 26.341997231138976 26.597001001489694 24.041430549186448 100-101 23.961103342498753 25.16860354313702 26.624584129233025 24.245708985131202 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 465.0 1 337.5 2 298.5 3 401.0 4 345.5 5 321.0 6 576.0 7 1112.0 8 1806.5 9 2177.5 10 1906.0 11 1632.5 12 1712.0 13 1825.0 14 1999.0 15 2230.0 16 2331.5 17 2487.0 18 2802.0 19 3139.5 20 3586.0 21 4349.5 22 5443.0 23 6741.0 24 8423.0 25 10605.0 26 13513.5 27 16972.5 28 20031.5 29 22788.5 30 25913.0 31 30334.5 32 36772.5 33 40145.5 34 40980.0 35 43783.0 36 48205.0 37 52509.0 38 55935.0 39 59252.5 40 62748.5 41 67289.5 42 70256.0 43 72664.0 44 76430.5 45 81493.5 46 84906.0 47 87594.0 48 90749.5 49 90556.5 50 89105.0 51 87792.5 52 87600.0 53 89512.0 54 90570.5 55 86360.5 56 79798.5 57 73420.0 58 67864.5 59 60391.5 60 49660.5 61 41644.5 62 35296.0 63 27797.0 64 20809.0 65 15746.0 66 11917.0 67 9307.0 68 7246.5 69 5511.0 70 4313.0 71 3233.0 72 2409.0 73 1863.5 74 1373.0 75 957.5 76 711.5 77 488.0 78 305.0 79 195.0 80 128.5 81 85.5 82 60.5 83 41.5 84 25.0 85 15.0 86 10.0 87 6.0 88 7.0 89 6.5 90 3.5 91 3.0 92 2.5 93 3.5 94 4.0 95 2.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14356431337446968 2 0.0513203070602908 3 0.15164135927984232 4 0.12720311782256097 5 0.19239937554079964 6 0.1962515051264389 7 0.16406758374964636 8 0.0685099175768531 9 0.12103142633589162 10-11 0.10073525970187831 12-13 0.15141354516456257 14-15 0.1373512011395676 16-17 0.1352180326055846 18-19 0.13215289723636628 20-21 0.13426535539623294 22-23 0.19558877315471604 24-25 0.21373106088062996 26-27 0.1476235467012723 28-29 0.1267060688437688 30-31 0.09818788368556847 32-33 0.12223262803463934 34-35 0.09106351498954747 36-37 0.08534745173343761 38-39 0.05088538920384766 40-41 0.058755331368056905 42-43 0.06119915551378503 44-45 0.039101186331650134 46-47 0.04937353189335483 48-49 0.04216632170086847 50-51 0.05297713698959801 52-53 0.026343595875984623 54-55 0.0510096514485457 56-57 0.02619862325717024 58-59 0.05156883154968688 60-61 0.03783785351055338 62-63 0.051506700427337855 64-65 0.04044736064921224 66-67 0.039101186331650134 68-69 0.06654243203580078 70-71 0.052438667262573166 72-73 0.051154624067360085 74-75 0.0436781790113613 76-77 0.02205654843390222 78-79 0.021518078706877376 80-81 0.025100973429004213 82-83 0.02191157581508784 84-85 0.019322779050545327 86-87 0.023299170880882628 88-89 0.0653619407111694 90-91 0.10974427244248625 92-93 0.0808533005501918 94-95 0.07534434103524533 96-97 0.06685308764754588 98-99 0.05564877525060588 100-101 0.1322771594810643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2414249.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.278332311362604 #Duplication Level Percentage of deduplicated Percentage of total 1 79.31708719382074 47.017846526446284 2 12.092369331477688 14.33630975326128 3 3.8152439012894375 6.784838874886042 4 1.5961932894127193 3.7847870497189664 5 0.826332043713999 2.449179274340292 6 0.4924712729140465 1.7515725461759157 7 0.33825103900363207 1.4035670246304681 8 0.24965879209000816 1.183948547357191 9 0.18330670477115166 0.9779504184292647 >10 0.9847053609583158 10.786936001931606 >50 0.0685634000278284 2.746190634095473 >100 0.031197923025271173 3.402905447733764 >500 0.0027998469667996602 1.1423732921383827 >1k 0.0016799081800769815 1.6979587338670725 >5k 1.3999234833974846E-4 0.533635874988134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7005 0.29015234136992496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5856 0.2425599016505754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3437 0.14236311167572194 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2933 0.1214870545664511 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2897 0.11999590763007462 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2527 0.10467023078398292 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2453 0.1016050954147646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.142074823268023E-5 2 0.0 0.0 0.0 0.0 4.142074823268023E-5 3 0.0 0.0 0.0 0.0 4.142074823268023E-5 4 0.0 0.0 0.0 0.0 4.142074823268023E-5 5 0.0 0.0 0.0 0.0 8.284149646536046E-5 6 0.0 0.0 0.0 0.0 2.4852448939608135E-4 7 0.0 0.0 0.0 0.0 2.4852448939608135E-4 8 0.0 0.0 0.0 0.0 2.4852448939608135E-4 9 0.0 0.0 0.0 0.0 2.4852448939608135E-4 10-11 0.0 0.0 0.0 0.0 2.4852448939608135E-4 12-13 0.0 0.0 0.0 0.0 7.869942164209243E-4 14-15 2.0710374116340114E-5 0.0 0.0 2.0710374116340114E-5 9.112564611189649E-4 16-17 4.142074823268023E-5 0.0 0.0 6.213112234902034E-5 0.0010355187058170056 18-19 4.142074823268023E-5 0.0 0.0 8.284149646536046E-5 0.001076939454049686 20-21 4.142074823268023E-5 0.0 0.0 1.2426224469804068E-4 0.0011390705763987064 22-23 4.142074823268023E-5 0.0 0.0 2.0710374116340112E-4 0.001284043195213087 24-25 4.142074823268023E-5 0.0 0.0 4.556282305594825E-4 0.001284043195213087 26-27 4.142074823268023E-5 0.0 0.0 7.662838423045841E-4 0.0013461743175621074 28-29 4.142074823268023E-5 0.0 0.0 0.0030030042468693163 0.0013668846916784474 30-31 4.142074823268023E-5 0.0 0.0 0.011162891648707322 0.0013668846916784474 32-33 4.142074823268023E-5 0.0 0.0 0.028911682266410797 0.0014911469363764881 34-35 4.142074823268023E-5 0.0 0.0 0.05428189055892743 0.001656829929307209 36-37 4.142074823268023E-5 0.0 0.0 0.10108733606185609 0.001656829929307209 38-39 4.142074823268023E-5 0.0 0.0 0.1718753947915066 0.0016982506775398893 40-41 4.142074823268023E-5 0.0 0.0 0.24274629501762246 0.0017603817998889095 42-43 4.142074823268023E-5 0.0 0.0 0.3154189977918599 0.0018018025481215897 44-45 4.142074823268023E-5 0.0 0.0 0.40528131108265963 0.0018639336704706101 46-47 4.142074823268023E-5 0.0 0.0 0.5092680995208034 0.0019053544187032903 48-49 4.142074823268023E-5 0.0 0.0 0.621414775360785 0.0019053544187032903 50-51 4.142074823268023E-5 0.0 0.0 0.7368129799370322 0.002029616663401331 52-53 4.142074823268023E-5 0.0 0.0 0.8489803661511303 0.002071037411634011 54-55 4.142074823268023E-5 0.0 0.0 0.9712544149340022 0.002071037411634011 56-57 4.142074823268023E-5 0.0 0.0 1.115005121675519 0.002174589282215712 58-59 4.142074823268023E-5 0.0 0.0 1.2623180127650464 0.0022781411527974123 60-61 4.142074823268023E-5 0.0 0.0 1.422450625432588 0.0023195619010300925 62-63 4.142074823268023E-5 0.0 0.0 1.5729529141360317 0.0025266656421934937 64-65 4.142074823268023E-5 0.0 0.0 1.7306624130319614 0.0025266656421934937 66-67 4.142074823268023E-5 0.0 0.0 1.913493595731012 0.002568086390426174 68-69 4.142074823268023E-5 0.0 0.0 2.1132451540831125 0.002609507138658854 70-71 4.142074823268023E-5 4.142074823268023E-5 0.0 2.3136387340328195 0.0026302175127751945 72-73 4.142074823268023E-5 4.142074823268023E-5 0.0 2.520659633699755 0.002775190131589575 74-75 4.142074823268023E-5 4.142074823268023E-5 0.0 2.720804689160066 0.002899452376287616 76-77 4.142074823268023E-5 4.142074823268023E-5 0.0 2.943689735400118 0.002940873124520296 78-79 4.142074823268023E-5 4.142074823268023E-5 0.0 3.192359197415014 0.002961583498636636 80-81 4.142074823268023E-5 4.142074823268023E-5 0.0 3.454221167742018 0.0030444249951019965 82-83 4.142074823268023E-5 4.142074823268023E-5 0.0 3.7096215013447247 0.003355080606847098 84-85 4.142074823268023E-5 4.142074823268023E-5 0.0 3.966865058243785 0.0033965013550797786 86-87 4.142074823268023E-5 4.142074823268023E-5 0.0 4.2364727084903 0.003437922103312459 88-89 6.213112234902034E-5 4.142074823268023E-5 0.0 4.521426745957024 0.003479342851545139 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8390 0.0 32.17528 1 GGTATCA 5755 0.0 31.933258 1 ATCAACG 9860 0.0 27.062195 3 TCAACGC 9955 0.0 26.756165 4 CAACGCA 10020 0.0 26.630064 5 TATCAAC 10065 0.0 26.605518 2 GTACATG 6250 0.0 26.052378 1 AACGCAG 10365 0.0 25.738869 6 TACATGG 6175 0.0 24.956583 2 ACGCAGA 11805 0.0 22.674103 7 CGCAGAG 11925 0.0 22.364805 8 ACATGGG 6630 0.0 21.665588 3 GAGTACT 7260 0.0 21.279545 12-13 GTATAGG 595 0.0 20.804518 1 AGAGTAC 9815 0.0 20.705414 10-11 GCAGAGT 12785 0.0 20.636871 9 GTACTTT 7480 0.0 20.525723 14-15 CATGGGG 4305 0.0 20.108297 4 GTGTAGC 1875 0.0 19.8059 1 AGTACTT 7565 0.0 19.29057 12-13 >>END_MODULE