FastQCFastQC Report
Thu 26 May 2016
SRR937912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414249
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68620.28422917437265166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61090.2530393509534435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35900.14870048615532203No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29510.12223262803463934No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27730.11485973484922227No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24900.10313766309937376No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24520.1015636746665319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.035.592551
GTATCAA84300.031.787331
ATCAACG97950.027.205833
TCAACGC99050.026.850734
TATCAAC101000.026.4816232
CAACGCA100800.026.3845735
AACGCAG104500.025.4498586
GTACATG64700.024.233281
TACATGG62450.023.8866212
ACGCAGA117850.022.4464767
CGCAGAG119150.022.1617138
GAGTACT77950.021.78213712-13
ACATGGG65550.021.739633
TAAGGTG12500.021.2765225
GTACTTT81550.020.79059214-15
GTAAGGT11950.020.6660754
ATTCGCG2600.020.09994580-81
GCAGAGT132300.019.7435599
AGAGTAC100600.019.73396710-11
GAGTACA48650.019.5321331
Produced by FastQC (version 0.11.5)