##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937912_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2414249 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4454772477901 33.0 31.0 34.0 31.0 34.0 2 32.67099665361775 34.0 31.0 34.0 31.0 34.0 3 32.7340763111013 34.0 31.0 34.0 31.0 34.0 4 36.20150407020982 37.0 35.0 37.0 35.0 37.0 5 36.16912381448641 37.0 35.0 37.0 35.0 37.0 6 36.19878852595569 37.0 37.0 37.0 35.0 37.0 7 36.152024086993514 37.0 36.0 37.0 35.0 37.0 8 36.136642492137305 37.0 36.0 37.0 35.0 37.0 9 37.81879375325412 39.0 38.0 39.0 35.0 39.0 10-11 37.78813701486466 39.0 38.0 39.0 35.0 39.0 12-13 37.701412737459975 39.0 37.0 39.0 35.0 39.0 14-15 38.963979896025634 40.0 38.0 41.0 35.5 41.0 16-17 38.82731855744788 40.0 38.0 41.0 35.0 41.0 18-19 39.07729898614434 40.0 38.5 41.0 36.0 41.0 20-21 39.04938761494775 40.0 39.0 41.0 35.5 41.0 22-23 38.945471034677865 40.0 38.0 41.0 35.0 41.0 24-25 38.8388070161777 40.0 38.0 41.0 35.0 41.0 26-27 38.652907591553316 40.0 38.0 41.0 34.0 41.0 28-29 38.53058984388106 40.0 38.0 41.0 34.0 41.0 30-31 38.297791984173955 40.0 38.0 41.0 34.0 41.0 32-33 38.09068223700206 40.0 38.0 41.0 33.5 41.0 34-35 37.933318394250136 40.0 37.0 41.0 33.0 41.0 36-37 37.93886836030583 40.0 37.5 41.0 33.0 41.0 38-39 37.78441473932473 40.0 37.0 41.0 33.0 41.0 40-41 37.610571858992174 40.0 37.0 41.0 33.0 41.0 42-43 37.370108882720885 40.0 37.0 41.0 32.0 41.0 44-45 37.098772951754356 40.0 36.0 41.0 31.0 41.0 46-47 36.725022771056345 39.0 36.0 41.0 30.5 41.0 48-49 36.32569962750321 39.0 35.0 40.5 30.0 41.0 50-51 35.919803632516775 39.0 35.0 40.0 29.0 41.0 52-53 35.367055759368654 38.0 34.5 40.0 27.5 41.0 54-55 35.01948224893124 38.0 34.0 40.0 26.5 41.0 56-57 34.63657042003538 38.0 34.0 40.0 26.0 41.0 58-59 34.157174757036245 37.5 33.0 40.0 24.5 41.0 60-61 33.63043103673233 37.0 33.0 39.5 23.0 41.0 62-63 33.12622703789046 36.0 32.0 39.0 22.0 40.0 64-65 32.59598264304966 35.5 31.5 39.0 20.5 40.0 66-67 31.998308790849663 35.0 31.0 38.0 18.5 40.0 68-69 31.360804125837888 35.0 30.0 38.0 15.0 40.0 70-71 30.686116055137642 34.0 29.5 37.0 9.5 39.0 72-73 30.049875344258194 34.0 29.0 36.5 7.5 39.0 74-75 29.407224565485997 34.0 28.0 36.0 4.5 39.0 76-77 28.098284808236432 31.5 26.5 34.5 2.0 36.5 78-79 28.768315530005395 33.5 28.0 35.0 2.0 37.0 80-81 28.40875568344442 33.0 27.5 35.0 2.0 37.0 82-83 27.78650545159178 33.0 26.0 35.0 2.0 36.0 84-85 27.13133110959143 32.5 25.5 35.0 2.0 36.0 86-87 26.415158502706223 32.0 24.5 35.0 2.0 35.0 88-89 25.580588000657762 31.5 21.5 34.0 2.0 35.0 90-91 24.950551496552343 31.0 19.5 34.0 2.0 35.0 92-93 24.03917077318868 31.0 12.5 34.0 2.0 35.0 94-95 23.115360511695357 30.0 2.0 34.0 2.0 35.0 96-97 21.950724635279954 29.5 2.0 34.0 2.0 35.0 98-99 20.386943724528827 29.0 2.0 34.0 2.0 35.0 100-101 16.162404540708103 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10754530237029769 1101 2 0.10878367499331887 1101 3 0.06155781712313768 1101 4 -0.01859427695848126 1101 5 0.003688583338288254 1101 6 -0.03266687938335622 1101 7 0.06009608393743093 1101 8 0.03957698669017873 1101 9 -0.015014734326108226 1101 10-11 0.07525027996342004 1101 12-13 0.12875583800784085 1101 14-15 0.022850455435872163 1101 16-17 0.06977250002803714 1101 18-19 0.12113962934600409 1101 20-21 0.16307933242998018 1101 22-23 0.23424396947051207 1101 24-25 0.21487360744461625 1101 26-27 0.12649785092069266 1101 28-29 0.20521897210245754 1101 30-31 0.2163455048131624 1101 32-33 0.22565032448040512 1101 34-35 0.18125290056283205 1101 36-37 0.25766818317308804 1101 38-39 0.2798571595948047 1101 40-41 0.15975633383802545 1101 42-43 0.2856563807946415 1101 44-45 0.2999219725547704 1101 46-47 0.2899218590790156 1101 48-49 0.34829752986687623 1101 50-51 0.0987644512550645 1101 52-53 -0.07266979407912544 1101 54-55 -0.28092632521565974 1101 56-57 -0.11844964579727701 1101 58-59 -0.049511358925769855 1101 60-61 -0.0032345689547526035 1101 62-63 0.019037290020698094 1101 64-65 -0.1746086850921884 1101 66-67 -0.43630677303313803 1101 68-69 -0.24756591695186003 1101 70-71 -0.23036452665851215 1101 72-73 -0.4846383877932432 1101 74-75 -0.639033017340843 1101 76-77 -0.602631933243952 1101 78-79 -0.6044818708384767 1101 80-81 -0.751020189574362 1101 82-83 -0.5262673630843011 1101 84-85 -0.4791103379963282 1101 86-87 -0.7960434270700887 1101 88-89 -0.8722407260411131 1101 90-91 -0.9575248987194875 1101 92-93 -1.1304498151093831 1101 94-95 -1.2713654403159005 1101 96-97 -1.0944054404437935 1101 98-99 -0.9319448077587893 1101 100-101 -1.3099324199465432 1102 1 0.10361800222750617 1102 2 0.14718950949355758 1102 3 0.11232536477619703 1102 4 0.06596933881945688 1102 5 0.09518764404923274 1102 6 0.08228330908886505 1102 7 0.13539592954126078 1102 8 0.1400813878411853 1102 9 0.09641120608306153 1102 10-11 0.15567552259339124 1102 12-13 0.1657030663536716 1102 14-15 0.25921308812215216 1102 16-17 0.2606155802457977 1102 18-19 0.27585935596628985 1102 20-21 0.2565191683053598 1102 22-23 0.3009801871972755 1102 24-25 0.2769266133302679 1102 26-27 0.19777700626569583 1102 28-29 0.11244005089440634 1102 30-31 0.07868573256783407 1102 32-33 0.17419010195860096 1102 34-35 0.17823530333171078 1102 36-37 0.20136670399842416 1102 38-39 0.16086973909219182 1102 40-41 0.12979937458239021 1102 42-43 0.09256078391175038 1102 44-45 -0.061448434764130866 1102 46-47 -0.02352476742989751 1102 48-49 0.1428116734596543 1102 50-51 0.28207289631152577 1102 52-53 0.02427434520901528 1102 54-55 0.21696606403812524 1102 56-57 0.2364737242096382 1102 58-59 0.2156649602899705 1102 60-61 0.11228323859317868 1102 62-63 0.08488592354748903 1102 64-65 0.1443323617354011 1102 66-67 0.077561281100607 1102 68-69 -0.07237078548825693 1102 70-71 -0.11725548462369417 1102 72-73 -0.10744922611321073 1102 74-75 -0.07247204786825634 1102 76-77 -0.2539972721640531 1102 78-79 -0.27671337850847166 1102 80-81 -0.36958622026190113 1102 82-83 -0.23902564394240855 1102 84-85 -0.1685403728247934 1102 86-87 -0.1499159692227785 1102 88-89 -0.21816376540164484 1102 90-91 -0.4663322105228076 1102 92-93 -0.39991971655256364 1102 94-95 -0.4328689001928403 1102 96-97 -0.5425518073319715 1102 98-99 -0.7857393672740969 1102 100-101 -0.8977294321812153 1103 1 0.18563281753061034 1103 2 0.15621547335344133 1103 3 0.1817501937300321 1103 4 0.09544778192172032 1103 5 0.0715150199497856 1103 6 0.10511546549275863 1103 7 0.11388874573976437 1103 8 0.0903218549874154 1103 9 0.07263036490124364 1103 10-11 0.2008336321453399 1103 12-13 0.24299615611617043 1103 14-15 0.2242276589755008 1103 16-17 0.2347162660784221 1103 18-19 0.2907768335744265 1103 20-21 0.29888038813709983 1103 22-23 0.2879073439170341 1103 24-25 0.3093162058133032 1103 26-27 0.3779441659518099 1103 28-29 0.313360445419832 1103 30-31 0.34694956251870224 1103 32-33 0.32818822875936604 1103 34-35 0.35419023668482197 1103 36-37 0.23295400689364243 1103 38-39 0.31629748496332866 1103 40-41 0.31713934925849685 1103 42-43 0.40212343858114963 1103 44-45 0.3573772176283114 1103 46-47 0.25034959638274046 1103 48-49 0.23337289164341968 1103 50-51 0.25683606844535234 1103 52-53 0.25291067263984957 1103 54-55 0.15037685223752106 1103 56-57 0.18567349459166138 1103 58-59 0.3815518597057661 1103 60-61 0.21844996431231323 1103 62-63 0.2945208144926852 1103 64-65 0.17768986538154508 1103 66-67 0.1855709931717584 1103 68-69 0.3012052946912611 1103 70-71 0.005764541029794401 1103 72-73 -0.0022166280517836867 1103 74-75 -0.10196449876544378 1103 76-77 -0.03277161757430491 1103 78-79 -0.07131570819703015 1103 80-81 -0.06905172675354976 1103 82-83 -0.1231451990987864 1103 84-85 -0.07642099345250841 1103 86-87 -0.26904210254951977 1103 88-89 -0.32822987670044057 1103 90-91 -0.4835321347159187 1103 92-93 -0.43040122763657607 1103 94-95 -0.3351598613198341 1103 96-97 -0.6787679997935783 1103 98-99 -0.7480166502013432 1103 100-101 -1.3506352256403993 1104 1 0.18612078341759997 1104 2 0.14450072375933587 1104 3 0.17085972289201834 1104 4 0.06839377560581994 1104 5 0.09665016955102601 1104 6 0.15105027253659387 1104 7 0.16058717033253345 1104 8 0.09921764260182186 1104 9 0.17165354105068076 1104 10-11 0.11308318164666531 1104 12-13 0.15421892547869476 1104 14-15 0.25555025170686463 1104 16-17 0.17535570656799848 1104 18-19 0.24169701056241166 1104 20-21 0.18213075834888315 1104 22-23 0.18022879479038068 1104 24-25 0.21089518135256213 1104 26-27 0.14566348297798726 1104 28-29 0.12103854850820284 1104 30-31 0.014072526353913872 1104 32-33 0.04428931887693466 1104 34-35 0.06947953711837584 1104 36-37 0.12930407558353352 1104 38-39 0.10257110269066771 1104 40-41 0.2452268262875208 1104 42-43 0.2555360775220805 1104 44-45 0.13637951918587987 1104 46-47 0.18714775513669224 1104 48-49 0.2049798783871708 1104 50-51 0.21255963175602233 1104 52-53 0.06642830899374985 1104 54-55 0.08925979394923189 1104 56-57 0.25645263427301046 1104 58-59 0.26575229147082524 1104 60-61 0.15490556566113156 1104 62-63 0.11180702834128198 1104 64-65 0.2282197588676027 1104 66-67 0.09226290560552286 1104 68-69 0.14785064072776777 1104 70-71 0.05861918607252292 1104 72-73 -0.17346009359086167 1104 74-75 -0.15753551698164614 1104 76-77 -0.1363073630827003 1104 78-79 -0.12805088634472384 1104 80-81 -0.17876242632000228 1104 82-83 -0.29705195925507866 1104 84-85 -0.07608111227710523 1104 86-87 -0.28439384834337744 1104 88-89 -0.36575418578713226 1104 90-91 -0.4125723495279594 1104 92-93 -0.40138822116010076 1104 94-95 -0.5053783308732598 1104 96-97 -0.8166897289948132 1104 98-99 -0.8544128392502124 1104 100-101 -1.2052887430983397 1105 1 0.15496191329871323 1105 2 0.1472546795511036 1105 3 0.11302665126500955 1105 4 0.04713430672585872 1105 5 0.12581863365124946 1105 6 0.11022408579906795 1105 7 0.12458903391164 1105 8 0.09621520959576912 1105 9 0.08764494788035648 1105 10-11 0.13039936890185544 1105 12-13 0.12035657150219237 1105 14-15 0.13558407221658086 1105 16-17 0.09685588774929954 1105 18-19 0.09784380192783004 1105 20-21 0.06382281915677623 1105 22-23 0.14068770114552365 1105 24-25 0.13704740815704497 1105 26-27 0.13127078544722792 1105 28-29 0.11790928859068117 1105 30-31 0.055577280206172475 1105 32-33 0.056453486391994545 1105 34-35 0.17457763385193203 1105 36-37 0.14363249689046143 1105 38-39 0.09098326426988734 1105 40-41 0.08975299404416148 1105 42-43 -0.07656622951983394 1105 44-45 -0.022219690518539892 1105 46-47 -0.07582913993345386 1105 48-49 0.12161059638278005 1105 50-51 0.06829013751068658 1105 52-53 -0.012859217104981724 1105 54-55 0.06258731797732509 1105 56-57 -0.02268218187110449 1105 58-59 0.07102639289784207 1105 60-61 -0.13701374826567303 1105 62-63 -0.06845585710036772 1105 64-65 -0.11769589692243443 1105 66-67 -0.2997819410805853 1105 68-69 -0.28214133552885556 1105 70-71 -0.4742186307790597 1105 72-73 -0.4375719601033836 1105 74-75 -0.38304411877965805 1105 76-77 -0.5086353702609294 1105 78-79 -0.5365293085144138 1105 80-81 -0.5320318860546145 1105 82-83 -0.4591919692631876 1105 84-85 -0.4505940796817285 1105 86-87 -0.576688466395936 1105 88-89 -0.8752463604804177 1105 90-91 -1.2034377922142703 1105 92-93 -1.0858989453676458 1105 94-95 -1.1204099081859091 1105 96-97 -0.9791424924410528 1105 98-99 -1.236399966750085 1105 100-101 -1.8469971102234233 1106 1 0.10667232281065253 1106 2 0.1351391916646989 1106 3 0.12863578819441557 1106 4 0.08908639035185928 1106 5 0.10482764723788307 1106 6 0.0115976938340836 1106 7 0.08945746663154353 1106 8 0.04254775485050999 1106 9 0.08308166397379324 1106 10-11 0.01315280192563506 1106 12-13 0.04370850588144748 1106 14-15 0.048700350174200935 1106 16-17 0.09711841456240222 1106 18-19 0.19397205449556054 1106 20-21 0.159259834142226 1106 22-23 0.2126591031582663 1106 24-25 0.1785575301348743 1106 26-27 0.17184886251553877 1106 28-29 0.22074477743354493 1106 30-31 0.18128667547789945 1106 32-33 0.1530741137264613 1106 34-35 0.28748030365497357 1106 36-37 0.14858583486658006 1106 38-39 0.11531008474637616 1106 40-41 0.1287090838193592 1106 42-43 0.02379463276368199 1106 44-45 0.16854673832972367 1106 46-47 0.15892004483860234 1106 48-49 0.06641819461613352 1106 50-51 0.019506777426521182 1106 52-53 -0.03547478120884762 1106 54-55 -0.09492488428887214 1106 56-57 -0.15785061249876975 1106 58-59 -0.09134918085027266 1106 60-61 -0.13642074652312886 1106 62-63 -0.1457607877225513 1106 64-65 -0.27135966533293754 1106 66-67 -0.46191013187730334 1106 68-69 -0.5177598975195572 1106 70-71 -1.0196590830225958 1106 72-73 -0.8085149743495705 1106 74-75 -0.5892880724434235 1106 76-77 -0.5403401406769994 1106 78-79 -0.5475847260814923 1106 80-81 -0.6906379334369674 1106 82-83 -0.6085981206599591 1106 84-85 -0.5843554263695587 1106 86-87 -0.6888689134786006 1106 88-89 -1.2002238688568667 1106 90-91 -1.4620664557585137 1106 92-93 -1.2661893089896985 1106 94-95 -1.2340581142858973 1106 96-97 -1.428130593550243 1106 98-99 -1.4841051237331442 1106 100-101 -2.1159887581844323 1107 1 -0.02486535682716351 1107 2 -0.07527677376340591 1107 3 -0.03364437583782376 1107 4 -0.10361582455890783 1107 5 -0.0741639141222592 1107 6 -0.020803614500607637 1107 7 0.004586423939024087 1107 8 -0.06431524375333453 1107 9 -0.031695578076295305 1107 10-11 0.050214328132831554 1107 12-13 -0.0927845619806078 1107 14-15 -0.0441141929377693 1107 16-17 -0.0753063959452902 1107 18-19 -0.041806084396853294 1107 20-21 -0.06889263722680283 1107 22-23 -0.1414060000157562 1107 24-25 -0.11678186189964634 1107 26-27 -0.1130635300324272 1107 28-29 -0.1141680609349578 1107 30-31 -0.16607598021563774 1107 32-33 -0.1420584361515651 1107 34-35 -0.1516466162526271 1107 36-37 -0.24433568660005278 1107 38-39 -0.2497726372555391 1107 40-41 -0.21928505809869137 1107 42-43 -0.1851066573666671 1107 44-45 -0.2351182223303283 1107 46-47 -0.36744578212507406 1107 48-49 -0.5117782643798918 1107 50-51 -0.5439468605805757 1107 52-53 -0.6013052928696538 1107 54-55 -0.46422283953294396 1107 56-57 -0.5996822699183753 1107 58-59 -0.5468472812832346 1107 60-61 -0.655762392180506 1107 62-63 -0.7293186102755698 1107 64-65 -0.8952639684671304 1107 66-67 -0.8757969216944659 1107 68-69 -0.7848346385906986 1107 70-71 -0.8974349660630629 1107 72-73 -1.2622362012774282 1107 74-75 -1.3308706090582945 1107 76-77 -1.3715261979366993 1107 78-79 -1.3004449435081717 1107 80-81 -1.1794827603954374 1107 82-83 -1.245270909928763 1107 84-85 -1.483260323931141 1107 86-87 -1.4900535701187678 1107 88-89 -1.6638978536677627 1107 90-91 -1.7344239845157965 1107 92-93 -1.747436517967337 1107 94-95 -1.9186641352409488 1107 96-97 -2.084529177178375 1107 98-99 -1.861413173840404 1107 100-101 -2.2531753556300274 1108 1 -0.042720956438607516 1108 2 -0.025906039397391112 1108 3 -0.027347686482563915 1108 4 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0.5979636411241316 2316 66-67 0.6201936381279793 2316 68-69 0.6775592545748417 2316 70-71 0.8306816248147513 2316 72-73 0.9029771543484664 2316 74-75 1.3242711253693642 2316 76-77 1.070816614615456 2316 78-79 1.0836712403644704 2316 80-81 1.1872927337937575 2316 82-83 1.2376042741751974 2316 84-85 1.3186125586903223 2316 86-87 1.5596820229229138 2316 88-89 1.7890926095275503 2316 90-91 1.9773078664207553 2316 92-93 1.8944285759220243 2316 94-95 2.3458544566968342 2316 96-97 2.581140741625557 2316 98-99 2.9103701676427924 2316 100-101 3.5627170251745532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 22.0 5 48.0 6 79.0 7 261.0 8 617.0 9 1141.0 10 1898.0 11 3122.0 12 4981.0 13 7437.0 14 10241.0 15 13672.0 16 17562.0 17 23520.0 18 24467.0 19 21506.0 20 21502.0 21 22723.0 22 24813.0 23 27130.0 24 30739.0 25 34611.0 26 39992.0 27 46929.0 28 56064.0 29 67399.0 30 80576.0 31 98023.0 32 120517.0 33 152943.0 34 201831.0 35 274255.0 36 368799.0 37 416296.0 38 191078.0 39 7447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53030554712118 18.392933407288872 14.021299361284276 24.05546168430567 2 16.159699282755902 19.465260845034017 39.44357864788261 24.931461224327474 3 20.09132439006608 22.407532294518052 29.181303313294936 28.319840002120934 4 13.30037344962075 15.93924899181857 35.680882825625204 35.07949473293547 5 14.059181774011764 36.74275325850181 34.27081922423902 14.927245743247413 6 34.11395079115235 35.17860989147544 16.37233866291111 14.335100654461105 7 29.93899966407773 31.68683097725214 19.616369314018563 18.757800044651564 8 26.762152536875856 35.32659638670245 18.814753573471503 19.096497502950193 9 25.94285013683344 16.529570893474535 19.668932243525834 37.85864672616619 10-11 25.65155872488712 25.96532089274967 26.634907998305064 21.74821238405815 12-13 26.177602115458853 23.058074879423156 27.31885720818415 23.445465796933842 14-15 22.654022016784516 24.865061557444985 25.36086791379017 27.120048511980333 16-17 22.716496432846025 27.94819109349574 26.366991352548556 22.96832112110968 18-19 22.708842377070468 26.572818296704277 28.23569565525346 22.482643670971804 20-21 22.91298453473523 25.762587040524814 28.749085119223412 22.57534330551654 22-23 22.675457253994928 25.740240546853286 28.623911618064252 22.960390581087534 24-25 22.719820526606615 25.8836769843433 28.651455943109323 22.745046545940763 26-27 22.356307160359414 26.246013236149203 28.500786218728102 22.89689338476329 28-29 22.35419338924008 26.75645106136728 28.357206131778177 22.53214941761446 30-31 22.561897631378105 26.125217077936803 28.352962253033397 22.959923037651695 32-33 22.336179905221044 26.123113233142064 28.465622228693064 23.075084632943828 34-35 22.98446661303565 26.311439893356575 28.20209890034324 22.50199459326453 36-37 23.01484883071438 25.911134003153386 28.24067544503311 22.833341721099124 38-39 22.49548864970569 26.237470686319647 28.35691993396539 22.910120730009275 40-41 22.980181442977845 26.084540564542653 28.230052829050763 22.705225163428743 42-43 22.66703090480523 26.11622662267366 28.073336960526625 23.143405511994487 44-45 22.720631285470404 26.329207618851164 28.240112813573248 22.710048282105177 46-47 23.06981144423559 26.610875610853245 27.615682080450792 22.703630864460376 48-49 22.991693020770555 26.471408127212136 27.643932757167384 22.89296609484992 50-51 22.94693784561854 26.765176148955117 27.602199531926352 22.685686473499988 52-53 23.15776392369669 26.59609370145606 27.504919119322302 22.741223255524947 54-55 22.71892112557561 26.69309918948134 27.58255088964846 23.005428795294588 56-57 22.893868496113047 26.825976812927095 27.455636132394584 22.824518558565277 58-59 22.92873468070425 26.537978471315494 27.384927517858227 23.14835933012203 60-61 22.633947109803543 26.748703737647062 27.674680866266733 22.94266828628266 62-63 22.64034510794981 26.73830522167386 27.800040393787643 22.821309276588693 64-65 22.397316175861327 26.782166117030975 27.64846378204597 23.172053925061732 66-67 22.423959720255617 26.755009905058913 27.487760561874925 23.333269812810542 68-69 22.75956109813307 26.456839465700536 27.67756739444072 23.106032041725673 70-71 22.653098255809617 26.62972555784513 27.31736377111675 23.399812415228503 72-73 22.66021105757441 26.657037240775967 27.297019876328015 23.385731825321606 74-75 22.543804593915517 27.03504160374838 27.137222978277396 23.28393082405871 76-77 22.708070988188176 26.66972694285245 27.305818329383353 23.316383739576022 78-79 22.68749233507569 26.71431211240342 27.43350988899532 23.164685663525567 80-81 22.841109888359536 26.809660248360107 27.18528870366032 23.163941159620034 82-83 22.707991920029006 26.91239446832755 27.16739006577925 23.212223545864195 84-85 22.677885130274085 26.916813795586403 27.139107415815435 23.266193658324077 86-87 22.774431393834803 26.905479356983264 27.05967658565943 23.260412663522505 88-89 22.628431109750352 27.105544339427606 26.77018602660577 23.49583852421627 90-91 22.705692809890184 26.95133650943664 26.860059965747475 23.482910714925705 92-93 22.684229584620113 26.670646813990125 27.045722529318716 23.59940107207105 94-95 22.522641579391404 26.54666279183211 27.194316669956685 23.736378958819802 96-97 22.692334301493418 26.27881436224267 27.157470388676714 23.871380947587202 98-99 22.44516502533713 26.554720017730293 26.804394047509316 24.195720909423258 100-101 23.009930102780768 25.80012286798568 26.75284686282738 24.43710016640617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 319.0 2 262.0 3 370.5 4 308.5 5 270.0 6 541.0 7 1089.0 8 1742.5 9 2089.0 10 1857.0 11 1578.0 12 1583.5 13 1708.0 14 1923.5 15 2096.5 16 2226.0 17 2453.5 18 2743.5 19 3137.5 20 3625.0 21 4238.0 22 5308.0 23 6609.0 24 8324.5 25 10624.5 26 13434.0 27 16714.0 28 19904.0 29 22975.0 30 26295.0 31 30741.0 32 37285.5 33 40508.0 34 41200.5 35 44223.5 36 48366.0 37 52602.5 38 56204.5 39 59324.5 40 63167.0 41 68114.0 42 70757.0 43 72765.5 44 76556.0 45 81941.0 46 85074.5 47 87914.0 48 91780.5 49 91951.0 50 90369.5 51 88600.5 52 88193.0 53 90095.5 54 91317.5 55 86959.0 56 79754.5 57 73046.0 58 67274.5 59 59803.0 60 49026.5 61 40643.0 62 34514.0 63 26981.0 64 19765.5 65 14805.0 66 11169.0 67 8789.0 68 6811.5 69 5073.5 70 3934.0 71 2817.5 72 2052.0 73 1651.5 74 1253.0 75 883.5 76 604.0 77 398.0 78 252.0 79 144.0 80 87.5 81 54.5 82 30.5 83 13.0 84 9.0 85 8.0 86 5.0 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0077042591712785215 2 0.011887754742779224 3 0.00898830236649161 4 4.142074823268022E-5 5 0.001076939454049686 6 0.002029616663401331 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.698357128862847E-4 14-15 0.0 16-17 0.002878742002171276 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0031065561174510167 26-27 0.01990266952580285 28-29 0.007020816825439298 30-31 0.0033757909809634386 32-33 0.0 34-35 0.00923682685588769 36-37 7.662838423045841E-4 38-39 0.0025059552680771536 40-41 0.0019467751669359705 42-43 0.0032101079880327175 44-45 2.071037411634011E-5 46-47 1.2426224469804068E-4 48-49 5.798904752575232E-4 50-51 0.004100654075035342 52-53 0.008242728898303365 54-55 0.00735218281130074 56-57 0.01673398228600281 58-59 0.02425184809023427 60-61 0.003997102204453642 62-63 0.021186712721015934 64-65 0.02191157581508784 66-67 0.013710267665017156 68-69 0.03193539688739645 70-71 0.016858244530700853 72-73 0.005011910536154307 74-75 0.01673398228600281 76-77 0.0216837616998081 78-79 0.027213431588870907 80-81 0.012198410354524326 82-83 0.03620173395536251 84-85 0.022760701153857782 86-87 0.0323703147438396 88-89 0.023278460506766286 90-91 0.02918091712992322 92-93 0.024976711184306173 94-95 0.015843436199000187 96-97 0.016236933307210648 98-99 0.012488355592153087 100-101 0.011432126512219741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2414249.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.5389433936745 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04013056951507 44.90346655274294 2 12.738924632891038 14.659685266964065 3 4.0216149128885075 6.941984184715472 4 1.710266301683975 3.936276636828131 5 0.9378713306852489 2.698206270342436 6 0.5603749317263927 1.9346028885503477 7 0.3654519259938614 1.4719402378009079 8 0.24065214728274087 1.1077496224054275 9 0.1964361198695426 1.0172454103482003 >10 1.0751524425471088 11.375670025070313 >50 0.07542030006820834 2.9662482632932035 >100 0.032933795152228114 3.5371918233869626 >500 0.0028912664824126714 1.1918476280061288 >1k 0.001734759889446684 1.7184199425598659 >5k 1.4456332412055703E-4 0.5394652469854304 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6862 0.28422917437265166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6109 0.2530393509534435 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3590 0.14870048615532203 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2951 0.12223262803463934 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2773 0.11485973484922227 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2490 0.10313766309937376 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2452 0.1015636746665319 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.142074823268023E-5 2 0.0 0.0 0.0 0.0 4.142074823268023E-5 3 0.0 0.0 0.0 0.0 8.284149646536046E-5 4 0.0 0.0 0.0 0.0 8.284149646536046E-5 5 0.0 0.0 0.0 0.0 8.284149646536046E-5 6 0.0 0.0 0.0 0.0 1.2426224469804068E-4 7 0.0 0.0 0.0 0.0 1.2426224469804068E-4 8 0.0 0.0 0.0 0.0 1.2426224469804068E-4 9 0.0 0.0 0.0 0.0 1.2426224469804068E-4 10-11 0.0 0.0 0.0 0.0 1.2426224469804068E-4 12-13 0.0 0.0 0.0 0.0 1.8639336704706101E-4 14-15 0.0 0.0 0.0 0.0 2.6923486351242143E-4 16-17 0.0 0.0 0.0 6.213112234902034E-5 2.8994523762876156E-4 18-19 0.0 0.0 0.0 8.284149646536046E-5 3.7278673409412203E-4 20-21 0.0 0.0 0.0 1.2426224469804068E-4 5.177593529085028E-4 22-23 0.0 0.0 0.0 2.0710374116340112E-4 6.006008493738633E-4 24-25 0.0 0.0 0.0 4.556282305594825E-4 6.627319717228836E-4 26-27 0.0 0.0 0.0 7.662838423045841E-4 8.077045905372644E-4 28-29 0.0 0.0 0.0 0.0030030042468693163 8.905460870026248E-4 30-31 0.0 0.0 0.0 0.01103862940400928 9.319668352353051E-4 32-33 0.0 0.0 0.0 0.028745999273480073 9.526772093516452E-4 34-35 0.0 0.0 0.0 0.05409549719188038 9.526772093516452E-4 36-37 0.0 0.0 0.0 0.10119088793243779 9.526772093516452E-4 38-39 0.0 0.0 0.0 0.17268309938204385 9.940979575843254E-4 40-41 0.0 0.0 0.0 0.24353328923404338 0.0010355187058170056 42-43 0.0 0.0 0.0 0.31593675714476843 0.0010355187058170056 44-45 0.0 0.0 0.0 0.4059026223061499 0.0010562290799333456 46-47 0.0 0.0 0.0 0.5098894107442936 0.001097649828166026 48-49 0.0 0.0 0.0 0.6221810592030896 0.0012426224469804068 50-51 0.0 0.0 0.0 0.7376621052758021 0.001284043195213087 52-53 0.0 0.0 0.0 0.8505336442098557 0.0015325676846091683 54-55 0.0 0.0 0.0 0.9731183486044728 0.0015739884328418486 56-57 0.0 0.0 0.0 1.1173453939506655 0.0016775403034235492 58-59 0.0 0.0 0.0 1.2655902518754278 0.0017810921740052496 60-61 0.0 0.0 0.0 1.426219913521762 0.0017810921740052496 62-63 0.0 0.0 0.0 1.5762458636205294 0.0018639336704706101 64-65 0.0 4.142074823268023E-5 0.0 1.733561865408249 0.0018639336704706101 66-67 0.0 4.142074823268023E-5 0.0 1.9164965999778811 0.0018846440445869502 68-69 0.0 4.142074823268023E-5 0.0 2.11552329523591 0.0019053544187032903 70-71 0.0 4.142074823268023E-5 0.0 2.3158340336891516 0.0019467751669359707 72-73 0.0 4.142074823268023E-5 0.0 2.5233726927089957 0.0020917477857503516 74-75 0.0 4.142074823268023E-5 0.0 2.7242426112633784 0.002216010030448392 76-77 0.0 4.142074823268023E-5 0.0 2.9480389139645498 0.0022988515269137524 78-79 0.0 4.142074823268023E-5 0.0 3.198033839922891 0.0023195619010300925 80-81 0.0 4.142074823268023E-5 0.0 3.461759743920366 0.002360982649262773 82-83 0.0 4.142074823268023E-5 0.0 3.718506251840634 0.002609507138658854 84-85 0.0 4.142074823268023E-5 0.0 3.9769717208125592 0.0026923486351242147 86-87 0.0 4.142074823268023E-5 0.0 4.247635600139008 0.0028580316280549358 88-89 0.0 4.142074823268023E-5 0.0 4.535199144744391 0.0030237146209856564 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5740 0.0 35.59255 1 GTATCAA 8430 0.0 31.78733 1 ATCAACG 9795 0.0 27.20583 3 TCAACGC 9905 0.0 26.85073 4 TATCAAC 10100 0.0 26.481623 2 CAACGCA 10080 0.0 26.384573 5 AACGCAG 10450 0.0 25.449858 6 GTACATG 6470 0.0 24.23328 1 TACATGG 6245 0.0 23.886621 2 ACGCAGA 11785 0.0 22.446476 7 CGCAGAG 11915 0.0 22.161713 8 GAGTACT 7795 0.0 21.782137 12-13 ACATGGG 6555 0.0 21.73963 3 TAAGGTG 1250 0.0 21.276522 5 GTACTTT 8155 0.0 20.790592 14-15 GTAAGGT 1195 0.0 20.666075 4 ATTCGCG 260 0.0 20.099945 80-81 GCAGAGT 13230 0.0 19.743559 9 AGAGTAC 10060 0.0 19.733967 10-11 GAGTACA 4865 0.0 19.532133 1 >>END_MODULE