FastQCFastQC Report
Thu 26 May 2016
SRR937911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017208
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69630.3451800706719386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54540.2703737046452324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35300.17499434862443536No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31390.1556111219071112No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30700.15219055248640695No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25350.1256687461084826No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24280.12036438483289776No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20220.10023755606759442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.039.2138861
GTATCAA63250.036.193571
ATCAACG75700.029.9446373
TCAACGC76500.029.6936134
CAACGCA76800.029.4538635
TATCAAC78150.029.1274932
AACGCAG79750.028.4792866
ACGCAGA89950.024.9813847
GTACATG57650.024.8183331
CGCAGAG91150.024.65258
TACATGG57800.023.1855742
ACCGTCG1452.3328597E-522.9343578
GCAGAGT100000.022.326029
ACATGGG58250.022.1090433
GAGTACT60200.021.81650412-13
TAAGGTG13100.021.4031285
CCGTCGT1353.7961453E-421.1120769
AGAGTAC80800.020.69375810-11
TATACCG1150.00371416920.6618275
GTAAGGT11300.020.1864224
Produced by FastQC (version 0.11.5)