FastQCFastQC Report
Thu 26 May 2016
SRR937911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017208
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67170.33298499708507995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58270.2888646089049815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36310.18000126908082856No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29880.14812552795745407No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29150.14450666465728867No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24920.12353708690427562No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24010.1190259011465352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.041.2822041
GTATCAA65300.034.227491
TATCAAC78700.028.0975592
ATCAACG78300.028.0554163
TCAACGC79200.027.7366054
CAACGCA80050.027.3232925
AACGCAG83250.026.3571686
GTACATG59400.023.2167761
ACGCAGA96900.022.5462887
CGCAGAG97550.022.3960578
TACATGG58200.022.1430132
ACATGGG57450.021.8499473
GTGTAGC11150.021.7513241
TAAGGTG11800.021.3565145
GAGTACT68250.020.59823812-13
GTACTTT69300.019.9777414-15
GCAGAGT108100.019.9027719
GTAAGGT11400.019.6033134
ACCGTCC2451.7598904E-719.3854088
AGAGTAC92150.019.06985510-11
Produced by FastQC (version 0.11.5)