##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2017208 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15995028772442 33.0 31.0 34.0 31.0 34.0 2 32.430827163088786 34.0 31.0 34.0 31.0 34.0 3 32.50641530273526 34.0 31.0 34.0 31.0 34.0 4 36.05436177131956 37.0 35.0 37.0 35.0 37.0 5 35.96395513006095 37.0 35.0 37.0 35.0 37.0 6 36.01860095736285 37.0 35.0 37.0 35.0 37.0 7 35.97500753516742 37.0 35.0 37.0 35.0 37.0 8 35.96354119158758 37.0 35.0 37.0 35.0 37.0 9 37.58858481624106 39.0 37.0 39.0 35.0 39.0 10-11 37.536342310758236 39.0 37.0 39.0 35.0 39.0 12-13 37.43332987971493 39.0 37.0 39.0 34.5 39.0 14-15 38.547035803942876 40.0 38.0 41.0 34.0 41.0 16-17 38.405402913333674 40.0 38.0 41.0 34.0 41.0 18-19 38.6854568294395 40.0 38.0 41.0 34.0 41.0 20-21 38.635418112559535 40.0 38.0 41.0 34.0 41.0 22-23 38.49597413851224 40.0 38.0 41.0 34.0 41.0 24-25 38.342721226566624 40.0 38.0 41.0 34.0 41.0 26-27 38.12537279249339 40.0 38.0 41.0 33.0 41.0 28-29 37.967087181887045 40.0 37.5 41.0 33.0 41.0 30-31 37.675695317488334 40.0 37.0 41.0 32.5 41.0 32-33 37.41207252796935 39.0 36.0 41.0 32.0 41.0 34-35 37.28677632648691 39.0 36.0 41.0 32.0 41.0 36-37 37.19887438479324 39.0 36.0 41.0 31.0 41.0 38-39 36.954814277952494 39.0 36.0 41.0 31.0 41.0 40-41 36.66329054812394 39.0 35.0 40.0 30.0 41.0 42-43 36.3343026599141 39.0 35.0 40.0 30.0 41.0 44-45 36.00884217195252 38.0 35.0 40.0 29.5 41.0 46-47 35.58769026297735 38.0 35.0 40.0 28.0 41.0 48-49 35.07909372756801 38.0 34.0 40.0 26.5 41.0 50-51 34.57772723487117 38.0 33.5 40.0 25.0 41.0 52-53 33.88080753199472 37.0 33.0 40.0 23.5 41.0 54-55 33.34230852742999 37.0 32.0 39.5 22.0 41.0 56-57 32.81896140606224 36.0 31.0 39.0 19.5 40.0 58-59 32.1479986198746 36.0 31.0 39.0 16.5 40.0 60-61 31.48858471709412 35.0 30.0 38.5 11.0 40.0 62-63 30.748866750478882 35.0 29.0 38.0 7.5 40.0 64-65 30.006757359677337 34.0 28.0 38.0 4.0 40.0 66-67 29.259320060202022 34.0 26.5 37.0 2.0 39.0 68-69 28.44940804319634 33.5 26.0 36.5 2.0 39.0 70-71 27.65619658458622 32.5 25.0 36.0 2.0 39.0 72-73 26.82294314716182 32.0 22.5 35.0 2.0 38.0 74-75 26.03546808261716 31.0 21.0 35.0 2.0 37.5 76-77 24.93517103838573 30.5 20.0 34.0 2.0 36.0 78-79 25.5083340438864 31.0 20.0 34.5 2.0 36.0 80-81 25.11542215775468 31.0 18.5 34.5 2.0 36.0 82-83 24.411861592855075 31.0 14.0 34.0 2.0 36.0 84-85 23.583801472133764 30.5 6.5 34.0 2.0 35.0 86-87 22.67224376464896 29.5 2.0 34.0 2.0 35.0 88-89 21.66156588710733 29.0 2.0 34.0 2.0 35.0 90-91 20.791821914249795 28.5 2.0 34.0 2.0 35.0 92-93 19.651347555631347 26.5 2.0 33.0 2.0 35.0 94-95 18.533107889716874 25.0 2.0 32.5 2.0 34.0 96-97 17.292233126182325 21.0 2.0 32.0 2.0 34.0 98-99 15.76976742110878 2.0 2.0 32.0 2.0 34.0 100-101 12.656185926290199 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20100101396073455 1101 2 0.1995646172905836 1101 3 0.23334646997290065 1101 4 0.037679167870969366 1101 5 0.014740111914285592 1101 6 0.1215367739344444 1101 7 0.14319222403565846 1101 8 0.11034441538776463 1101 9 0.12493099463769397 1101 10-11 0.24832609035500042 1101 12-13 0.31255461135953766 1101 14-15 0.37389149910072916 1101 16-17 0.48281456386357036 1101 18-19 0.33885098415844794 1101 20-21 0.31460618700253207 1101 22-23 0.42718352611421295 1101 24-25 0.45047950971261486 1101 26-27 0.4756674003436885 1101 28-29 0.5311321989893898 1101 30-31 0.6211420764496367 1101 32-33 0.6440087529939547 1101 34-35 0.7093878376137184 1101 36-37 0.9025927515861198 1101 38-39 0.9626457088237856 1101 40-41 0.9844380621046795 1101 42-43 1.08198989805053 1101 44-45 1.1480960866425036 1101 46-47 1.3249400848222948 1101 48-49 1.5411190608064516 1101 50-51 1.4020877662976403 1101 52-53 1.5252845212017547 1101 54-55 1.6934624355884083 1101 56-57 1.8247673071911095 1101 58-59 1.9462883985871215 1101 60-61 1.9548048820458312 1101 62-63 2.2865724651715844 1101 64-65 2.3017765561341292 1101 66-67 2.4687084570127915 1101 68-69 3.036646289515719 1101 70-71 3.2035488559978056 1101 72-73 3.279038925949042 1101 74-75 3.3718866562123253 1101 76-77 3.307952551991413 1101 78-79 3.5910153171656525 1101 80-81 3.753802863936979 1101 82-83 3.8236134076685744 1101 84-85 4.035289826289532 1101 86-87 4.401315583843093 1101 88-89 4.6847407657966365 1101 90-91 4.847528341902265 1101 92-93 5.051626156363724 1101 94-95 5.223565143869607 1101 96-97 5.465788506709096 1101 98-99 5.564600278142445 1101 100-101 4.849057312607011 1102 1 0.26356843575145916 1102 2 0.2891007553704128 1102 3 0.3099376242339531 1102 4 0.1611958884750706 1102 5 0.03847258224869421 1102 6 0.15551735645871645 1102 7 0.18580279577244596 1102 8 0.18046307773943937 1102 9 0.3271963668059783 1102 10-11 0.324108183127386 1102 12-13 0.43283508600894294 1102 14-15 0.5060382089173387 1102 16-17 0.5612935282648621 1102 18-19 0.5095629582684822 1102 20-21 0.4497194556109463 1102 22-23 0.5194165358229625 1102 24-25 0.6182249250308445 1102 26-27 0.579496958056744 1102 28-29 0.5052422313518505 1102 30-31 0.646222982598502 1102 32-33 0.64508750164552 1102 34-35 0.878751375909296 1102 36-37 0.8351709608633513 1102 38-39 0.924080444530361 1102 40-41 1.0693895184153561 1102 42-43 1.1467148171443782 1102 44-45 1.1273301750998925 1102 46-47 1.1482949931286655 1102 48-49 1.4451104308172376 1102 50-51 1.6073196972577293 1102 52-53 1.7852629462287197 1102 54-55 1.8536566103456877 1102 56-57 1.898931276986147 1102 58-59 2.1873887222117077 1102 60-61 2.180802724548524 1102 62-63 2.4653750541683443 1102 64-65 2.5248078398450247 1102 66-67 2.6267451344669475 1102 68-69 2.914208317563183 1102 70-71 3.1571626639805466 1102 72-73 3.244518969098987 1102 74-75 3.3702685332349773 1102 76-77 3.319818787158617 1102 78-79 3.603420926658643 1102 80-81 3.842529940528131 1102 82-83 3.943354507992197 1102 84-85 4.256163612697296 1102 86-87 4.26647200239757 1102 88-89 4.549088122862443 1102 90-91 4.731832549022009 1102 92-93 4.94186348106707 1102 94-95 5.001342921647385 1102 96-97 4.994105658812654 1102 98-99 5.115301464765961 1102 100-101 4.4267272155196355 1103 1 0.2883872515589232 1103 2 0.30022756657926664 1103 3 0.2847617578164616 1103 4 0.13643025041803014 1103 5 0.06992089094300269 1103 6 0.1558389552351258 1103 7 0.13846953334277856 1103 8 0.1729624826550875 1103 9 0.20145884205300035 1103 10-11 0.3405413741469303 1103 12-13 0.3302172213235295 1103 14-15 0.4411233692862453 1103 16-17 0.46638137284402603 1103 18-19 0.4634464534141145 1103 20-21 0.3378980416905293 1103 22-23 0.46041455558482625 1103 24-25 0.5292864035748863 1103 26-27 0.5132032953252335 1103 28-29 0.6105405078862773 1103 30-31 0.6432602222174424 1103 32-33 0.753186473035889 1103 34-35 0.7368440166150876 1103 36-37 0.8200735442891158 1103 38-39 0.8039367126677774 1103 40-41 0.9265173627919339 1103 42-43 1.0252416415210632 1103 44-45 1.080245075505566 1103 46-47 1.1695382382882187 1103 48-49 1.3035056782457133 1103 50-51 1.482527976780247 1103 52-53 1.5583218317243634 1103 54-55 1.7819780673147605 1103 56-57 1.7769516465968351 1103 58-59 1.7226164495972398 1103 60-61 1.9201456501351224 1103 62-63 2.2072502535849132 1103 64-65 2.266095551733031 1103 66-67 2.648864546371673 1103 68-69 2.863466614558387 1103 70-71 2.8022454946575266 1103 72-73 3.2476805129756947 1103 74-75 3.431208969089951 1103 76-77 3.3744449982185785 1103 78-79 3.5248052194705295 1103 80-81 3.638194434503852 1103 82-83 3.8304391517772416 1103 84-85 4.053862788695394 1103 86-87 4.250259119201235 1103 88-89 4.342850879191861 1103 90-91 4.410257829218107 1103 92-93 4.344746981277247 1103 94-95 4.678684388644264 1103 96-97 4.806702201752426 1103 98-99 5.10678036332814 1103 100-101 4.496690143030763 1104 1 0.22452936705283832 1104 2 0.15014300434735617 1104 3 0.18587329877011172 1104 4 0.11707858032058738 1104 5 0.13192700319770978 1104 6 0.15387070096362976 1104 7 0.19236966447128623 1104 8 0.20300980248075007 1104 9 0.1353448149113632 1104 10-11 0.18816033837500612 1104 12-13 0.35009544852555763 1104 14-15 0.48924901703506407 1104 16-17 0.46687476308638765 1104 18-19 0.36468416111163293 1104 20-21 0.4431473742406027 1104 22-23 0.5035236490893666 1104 24-25 0.47685370709837827 1104 26-27 0.40424776492341863 1104 28-29 0.5134488967674784 1104 30-31 0.556171338741386 1104 32-33 0.5965180233187084 1104 34-35 0.5834771290305909 1104 36-37 0.5709250039759866 1104 38-39 0.6639047610393902 1104 40-41 0.8009553171447763 1104 42-43 0.8533484080385563 1104 44-45 0.6630657818164494 1104 46-47 0.8825593095937947 1104 48-49 1.053067875664432 1104 50-51 1.1667685697294488 1104 52-53 1.3598228047074414 1104 54-55 1.4530392105579253 1104 56-57 1.5438188533792143 1104 58-59 1.6008907485691708 1104 60-61 1.5648948582431181 1104 62-63 1.8396146900056287 1104 64-65 1.936601481702663 1104 66-67 2.175883068339541 1104 68-69 2.3139851054820753 1104 70-71 2.543337671470333 1104 72-73 2.6898660287681615 1104 74-75 2.613298094020763 1104 76-77 2.7643759899183706 1104 78-79 2.900425506127739 1104 80-81 2.9651207260612686 1104 82-83 3.2198697218598795 1104 84-85 3.531117219920855 1104 86-87 3.699286427999578 1104 88-89 3.856553436648806 1104 90-91 3.9609588174844674 1104 92-93 4.291798075757168 1104 94-95 4.535389348443374 1104 96-97 4.718671114054462 1104 98-99 4.538842822101726 1104 100-101 3.7516341281805 1105 1 0.18014520669716205 1105 2 0.20882630616392106 1105 3 0.18458411538529873 1105 4 0.1566476509171224 1105 5 0.04065923492078838 1105 6 -0.001649519154760526 1105 7 0.09494008840488277 1105 8 0.0746388068666235 1105 9 0.11965873208387734 1105 10-11 0.13231201803024817 1105 12-13 0.27982528008651997 1105 14-15 0.32935667301250504 1105 16-17 0.38657269092266944 1105 18-19 0.37736036732802347 1105 20-21 0.3258966036327493 1105 22-23 0.4151545068036455 1105 24-25 0.32422218442616213 1105 26-27 0.3832035772973441 1105 28-29 0.4285733294402334 1105 30-31 0.4782783389256977 1105 32-33 0.6496357392034611 1105 34-35 0.6195577162301973 1105 36-37 0.6409719074409992 1105 38-39 0.6396139391900704 1105 40-41 0.6951098989901041 1105 42-43 0.7457191612943532 1105 44-45 0.8876123018868682 1105 46-47 0.9354802366245849 1105 48-49 1.0540907703800997 1105 50-51 1.0960147029430232 1105 52-53 1.1856463393305674 1105 54-55 1.3509378721900518 1105 56-57 1.4879231815120306 1105 58-59 1.6274720093823802 1105 60-61 1.6795247475423913 1105 62-63 1.8067018178252106 1105 64-65 1.9771728261905892 1105 66-67 2.170964110724757 1105 68-69 2.6343677974313984 1105 70-71 2.8260086944770606 1105 72-73 2.9701353621911046 1105 74-75 2.80682402076134 1105 76-77 2.890246010498462 1105 78-79 2.9596485134687534 1105 80-81 3.2878053129879703 1105 82-83 3.0601970787182644 1105 84-85 3.299065738123698 1105 86-87 3.5969967018543088 1105 88-89 3.5599412089582465 1105 90-91 3.5796561007290535 1105 92-93 3.945777589350108 1105 94-95 4.068976088795118 1105 96-97 4.197413335421306 1105 98-99 4.1527418177402975 1105 100-101 3.3808172278620727 1106 1 0.11834724999474133 1106 2 0.1011733322689139 1106 3 0.09625448246968205 1106 4 0.14210346439841715 1106 5 -0.01744026585387104 1106 6 0.05468976966860595 1106 7 0.11433160142696863 1106 8 0.15114500439192824 1106 9 0.24915912910019244 1106 10-11 0.19316905340958357 1106 12-13 0.24547478554493551 1106 14-15 0.1085859267640572 1106 16-17 0.1740454895419603 1106 18-19 0.17257596509139006 1106 20-21 0.12321339617248128 1106 22-23 0.044035102244748714 1106 24-25 0.0733699112808921 1106 26-27 -0.11173661031799753 1106 28-29 0.16597670537863252 1106 30-31 0.19406688592444254 1106 32-33 0.3747929149975846 1106 34-35 0.18167004904316997 1106 36-37 0.3906829119290762 1106 38-39 0.4063720005341196 1106 40-41 0.5065999779961103 1106 42-43 0.3573485515861634 1106 44-45 0.45610987834623273 1106 46-47 0.6057602667772315 1106 48-49 0.7019575249347483 1106 50-51 0.961423963256749 1106 52-53 1.1653433317664081 1106 54-55 1.0994841892077147 1106 56-57 1.320782253224607 1106 58-59 1.4773407414167181 1106 60-61 1.4185861999796394 1106 62-63 1.650093715357226 1106 64-65 1.6300439726923095 1106 66-67 1.9618932073863355 1106 68-69 2.064610509521245 1106 70-71 1.8940963803991409 1106 72-73 2.4085985681702375 1106 74-75 2.519385185551805 1106 76-77 2.4823546551548787 1106 78-79 2.761991032371782 1106 80-81 2.598706978985181 1106 82-83 3.2777083185224996 1106 84-85 3.175961742408237 1106 86-87 3.3410426831614366 1106 88-89 3.437566997412059 1106 90-91 3.4578244454579092 1106 92-93 3.367508972864929 1106 94-95 3.7713256701255915 1106 96-97 4.191011907045354 1106 98-99 4.2952775430785675 1106 100-101 3.5563574177334747 1107 1 0.26648430961917313 1107 2 0.22297563136852716 1107 3 0.22711357952280764 1107 4 0.048507852827576414 1107 5 -0.042703524823885175 1107 6 0.012515399532773586 1107 7 0.1837022173680154 1107 8 0.14265104020549302 1107 9 0.17026662641035983 1107 10-11 0.3267589775525863 1107 12-13 0.24881411817083432 1107 14-15 0.4696014403929567 1107 16-17 0.3694714187962731 1107 18-19 0.19677978674744168 1107 20-21 0.3601949723532343 1107 22-23 0.4492919105390385 1107 24-25 0.37190730673439276 1107 26-27 0.45793635027131785 1107 28-29 0.4516091793605028 1107 30-31 0.47923784114149015 1107 32-33 0.6052350571143421 1107 34-35 0.5501564053716024 1107 36-37 0.6439603229922284 1107 38-39 0.5790304914206956 1107 40-41 0.7164782522680042 1107 42-43 0.7064100028897187 1107 44-45 0.8217255608745049 1107 46-47 0.9690804197222533 1107 48-49 1.0408984323133907 1107 50-51 1.0781883338603748 1107 52-53 1.3794818834173626 1107 54-55 1.4274744590281543 1107 56-57 1.5277335949405355 1107 58-59 1.7703415120508694 1107 60-61 1.8103837472456128 1107 62-63 1.8753835648989075 1107 64-65 2.15490357201179 1107 66-67 2.3077317829195643 1107 68-69 2.5705019215052225 1107 70-71 2.7580148212068067 1107 72-73 2.896830147428606 1107 74-75 2.7887370896090786 1107 76-77 2.86179620390795 1107 78-79 2.9196444390425675 1107 80-81 2.982541757975671 1107 82-83 3.492912708921331 1107 84-85 3.6818814684268624 1107 86-87 3.7617707724072744 1107 88-89 3.8445693043903617 1107 90-91 3.7983924249930183 1107 92-93 4.053601242148638 1107 94-95 4.028080167748442 1107 96-97 4.249271726475673 1107 98-99 3.8450250557521795 1107 100-101 3.467503914548759 1108 1 0.2507338925909153 1108 2 0.23429541539724852 1108 3 0.20271703631036075 1108 4 0.18530039880462112 1108 5 0.06695957869786184 1108 6 0.17958393767332126 1108 7 0.21856629993181542 1108 8 0.18619762525067785 1108 9 0.255996724678333 1108 10-11 0.3079043632192864 1108 12-13 0.3024738335003221 1108 14-15 0.4626021211493736 1108 16-17 0.4490068934809628 1108 18-19 0.4340600349377226 1108 20-21 0.41794935959450896 1108 22-23 0.36446550342591166 1108 24-25 0.4494275345539478 1108 26-27 0.5531858656870767 1108 28-29 0.6032014218552888 1108 30-31 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2.6108006850482113 2311 74-75 2.8766102453420856 2311 76-77 2.817534943266846 2311 78-79 2.8909702461603466 2311 80-81 2.9352424761785585 2311 82-83 2.9734397734678097 2311 84-85 3.4564724599139787 2311 86-87 3.558590487420698 2311 88-89 3.6903805524112876 2311 90-91 3.9005022893834607 2311 92-93 4.111459024209626 2311 94-95 3.756277088424884 2311 96-97 4.181314359733289 2311 98-99 3.8402087367140663 2311 100-101 3.4490107638638303 2312 1 -0.08410556698757432 2312 2 -0.06609686498566703 2312 3 -0.09778720472186819 2312 4 -0.025900125970274246 2312 5 0.00153925240457653 2312 6 0.012113570080025227 2312 7 0.01625898142189186 2312 8 -0.04522364318450656 2312 9 -0.06889774892543699 2312 10-11 0.007959228583125366 2312 12-13 0.03254228280352578 2312 14-15 -0.05697844917167316 2312 16-17 -0.014079308785930778 2312 18-19 0.02813648036828198 2312 20-21 -0.04224444847056219 2312 22-23 -9.968197250387334E-4 2312 24-25 0.09029958257902848 2312 26-27 0.04191012751332579 2312 28-29 -0.018968552063917343 2312 30-31 0.03592965601098541 2312 32-33 0.15982652297915934 2312 34-35 0.014816182243386322 2312 36-37 -0.043658814925564116 2312 38-39 0.15477127981910854 2312 40-41 0.14697146405921302 2312 42-43 0.4619900143576601 2312 44-45 0.5176736300614095 2312 46-47 0.49325903968641427 2312 48-49 0.6553726452468851 2312 50-51 0.7489897804464078 2312 52-53 0.7564536695718616 2312 54-55 0.8228504856121006 2312 56-57 0.9411587621060988 2312 58-59 1.2890426096322258 2312 60-61 1.5779217968828299 2312 62-63 1.7492960292873398 2312 64-65 1.8575639990345962 2312 66-67 2.2315981949437464 2312 68-69 2.624104164526255 2312 70-71 2.7204482823128764 2312 72-73 2.69284661792123 2312 74-75 2.8437963030166387 2312 76-77 3.0181798742813832 2312 78-79 2.987537588527637 2312 80-81 2.8772134542829058 2312 82-83 2.8717572912218756 2312 84-85 3.134580498166759 2312 86-87 3.3885908566560126 2312 88-89 3.5840057047208553 2312 90-91 3.6449612981097204 2312 92-93 3.7083703708108153 2312 94-95 3.6081324958760526 2312 96-97 3.4978856406105905 2312 98-99 3.5101794440458445 2312 100-101 3.371187652194765 2313 1 -0.2800436937503221 2313 2 -0.2623898944325731 2313 3 -0.27619610308551046 2313 4 -0.15061056583730448 2313 5 -0.15965766691194005 2313 6 -0.1366309116038309 2313 7 -0.239209263800646 2313 8 -0.1815116815620641 2313 9 -0.218219047612898 2313 10-11 -0.29095565445721405 2313 12-13 -0.23031783226345937 2313 14-15 -0.2756553831810038 2313 16-17 -0.1640723589421711 2313 18-19 -0.3449946225258529 2313 20-21 -0.2955740645387621 2313 22-23 -0.21933006693638646 2313 24-25 -0.19770054079571509 2313 26-27 -0.16302153769727568 2313 28-29 -0.28222853350162325 2313 30-31 -0.179172915270982 2313 32-33 -0.22917876410947713 2313 34-35 -0.20918118763130877 2313 36-37 -0.18548830221136114 2313 38-39 -0.2042358178340038 2313 40-41 -0.1437577247638373 2313 42-43 0.05613936750145143 2313 44-45 0.017493074322260327 2313 46-47 0.034607004944398057 2313 48-49 0.23010639813447398 2313 50-51 0.13136057043334404 2313 52-53 0.2536624160403562 2313 54-55 0.3980336750816704 2313 56-57 0.46568359908520307 2313 58-59 0.9699805901878804 2313 60-61 0.9538612775632735 2313 62-63 1.1704007820591649 2313 64-65 1.1064012521198627 2313 66-67 1.398863209109809 2313 68-69 1.6955474568054392 2313 70-71 2.019104599120002 2313 72-73 2.0283975923213724 2313 74-75 2.3957274583049895 2313 76-77 2.49218555592887 2313 78-79 2.412083114811068 2313 80-81 2.5258930440538556 2313 82-83 2.7442415779656173 2313 84-85 2.999616935776988 2313 86-87 3.236090655370912 2313 88-89 3.2177695678565996 2313 90-91 3.5793833134522544 2313 92-93 3.8244043212982852 2313 94-95 3.5599650715362188 2313 96-97 3.7080821629648533 2313 98-99 3.474370624433748 2313 100-101 2.8790286581956614 2314 1 -0.18311015392662355 2314 2 -0.18537974198949314 2314 3 -0.2213744747838149 2314 4 -0.14006880237827346 2314 5 -0.11408234876816437 2314 6 -0.20499760408178958 2314 7 -0.2258675613501353 2314 8 -0.11752704319395946 2314 9 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2314 74-75 3.419763934359146 2314 76-77 3.3726178560281994 2314 78-79 3.418118167333141 2314 80-81 3.4159742714200867 2314 82-83 3.437973539389464 2314 84-85 3.899310719947625 2314 86-87 4.2671220904007505 2314 88-89 4.525807733377402 2314 90-91 4.821846317261375 2314 92-93 4.758415885541037 2314 94-95 4.665301361436178 2314 96-97 4.858603472927232 2314 98-99 4.720093379828699 2314 100-101 4.204126721566306 2315 1 -0.24725249668307825 2315 2 -0.2550323122798517 2315 3 -0.2733318792119164 2315 4 -0.23189635160067468 2315 5 -0.1575704357979646 2315 6 -0.15006387237260554 2315 7 -0.2606220239257908 2315 8 -0.16971427984454124 2315 9 -0.19623838774337798 2315 10-11 -0.2798735299631616 2315 12-13 -0.2757442632559517 2315 14-15 -0.2613327063231594 2315 16-17 -0.23945311654419044 2315 18-19 -0.31675733115648796 2315 20-21 -0.2671217422240204 2315 22-23 -0.24459235796505396 2315 24-25 -0.2497336491366582 2315 26-27 -0.19145361264369143 2315 28-29 -0.1941161722885738 2315 30-31 -0.13174063201029895 2315 32-33 -0.10427537715307267 2315 34-35 -0.1480343299644673 2315 36-37 -0.051195587564919265 2315 38-39 -0.21072286049169975 2315 40-41 0.13207918268427932 2315 42-43 0.2556408733994786 2315 44-45 0.28911161779667083 2315 46-47 0.3010700622580842 2315 48-49 0.24687482080607737 2315 50-51 0.48021484194666186 2315 52-53 0.4453647914070942 2315 54-55 0.5775819835503242 2315 56-57 0.6617749809096765 2315 58-59 0.9991635699385668 2315 60-61 1.2262743355583545 2315 62-63 1.3830342638083017 2315 64-65 1.6233637769666949 2315 66-67 1.9140340365457753 2315 68-69 2.2740612026269886 2315 70-71 2.5301506093176656 2315 72-73 2.6261503291231065 2315 74-75 2.9765813326583874 2315 76-77 3.1294170592764985 2315 78-79 2.8954327693403457 2315 80-81 3.1038831812492447 2315 82-83 3.4474024063166055 2315 84-85 3.648598240057943 2315 86-87 3.8493483985291483 2315 88-89 4.184521247512084 2315 90-91 4.691921204260531 2315 92-93 4.80013522209202 2315 94-95 5.0195111216445625 2315 96-97 5.290324150166182 2315 98-99 5.321958851367809 2315 100-101 4.986936228959243 2316 1 -0.2636657744305708 2316 2 -0.27988764101292674 2316 3 -0.1771056507913613 2316 4 -0.1289951664893536 2316 5 -0.13278319907251301 2316 6 -0.29660796049258664 2316 7 -0.2711788070343175 2316 8 -0.3091416861986289 2316 9 -0.1777955413810588 2316 10-11 -0.2274564576866922 2316 12-13 -0.31503868650112565 2316 14-15 -0.24844391744912286 2316 16-17 -0.13966689828892953 2316 18-19 -0.2547528654071556 2316 20-21 -0.2392799678743316 2316 22-23 -0.13752533874603756 2316 24-25 -0.07940556593637638 2316 26-27 -0.18872052361387404 2316 28-29 -0.1938922058418271 2316 30-31 -0.07839539389196659 2316 32-33 -0.06485718552935538 2316 34-35 -0.016938397831445684 2316 36-37 -0.09678784253554085 2316 38-39 -0.0868160670044773 2316 40-41 -0.02239481140665589 2316 42-43 -0.0896883252681775 2316 44-45 0.10570967513751128 2316 46-47 -0.15026966254156804 2316 48-49 0.2751284451050253 2316 50-51 0.3982160602310074 2316 52-53 0.49010094942710936 2316 54-55 0.8559703369071059 2316 56-57 1.080945790971775 2316 58-59 1.3680035226815477 2316 60-61 1.5346679849601763 2316 62-63 1.7658755628718943 2316 64-65 2.0669885415060847 2316 66-67 2.375766425256078 2316 68-69 2.6771572550364944 2316 70-71 2.751562635801772 2316 72-73 2.899748671635603 2316 74-75 3.501805541598035 2316 76-77 3.5220832008651968 2316 78-79 3.6097679798920836 2316 80-81 3.540362067877023 2316 82-83 3.9852116090193377 2316 84-85 4.111608178387147 2316 86-87 4.4635392773040365 2316 88-89 4.675267330281702 2316 90-91 5.071483223275855 2316 92-93 5.009833871309542 2316 94-95 5.637244045779202 2316 96-97 5.890097467798906 2316 98-99 6.04490964082771 2316 100-101 5.746868641336855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 20.0 5 48.0 6 137.0 7 320.0 8 810.0 9 1362.0 10 2474.0 11 3911.0 12 5994.0 13 8734.0 14 11985.0 15 16361.0 16 21501.0 17 27732.0 18 29605.0 19 28865.0 20 30354.0 21 32964.0 22 36782.0 23 40615.0 24 43518.0 25 48140.0 26 53382.0 27 60216.0 28 70499.0 29 82097.0 30 94132.0 31 107251.0 32 122986.0 33 142847.0 34 170341.0 35 206866.0 36 237342.0 37 209792.0 38 65935.0 39 1281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.248390845168174 18.782247542147363 14.261642825132558 23.707718787551904 2 16.329963742481883 19.781046832338355 39.22688638996986 24.662103035209903 3 20.2002515413975 22.861513918310024 29.35231312491851 27.58592141537397 4 13.467335786596813 16.124762477462316 35.70271721321431 34.70518452272656 5 13.971023584219749 37.04828261161945 34.15495876074283 14.825735043417973 6 34.008738811267854 35.39253264908726 16.226338582833304 14.372389956811594 7 29.73084580271345 31.971764934503533 19.682600901840562 18.614788360942452 8 26.938911888304634 35.290335469792055 18.72084140350912 19.04991123839419 9 26.013133003636707 16.54990462064398 19.700744791811257 37.73621758390806 10-11 25.71611355893889 26.117956105666845 26.49010414394549 21.675826191448774 12-13 26.267742344864782 23.146051373978292 27.372387973872797 23.21381830728413 14-15 22.59724827583472 24.82478257076117 25.335984192011928 27.24198496139218 16-17 22.596950835015527 28.11224722487716 26.343490606818932 22.947311333288386 18-19 22.716026309631925 26.50229921753235 28.517634274700477 22.264040198135245 20-21 22.88313847654778 25.687261799477294 29.043757510380686 22.385842213594234 22-23 22.595296556667822 25.67899710638939 28.957819317143592 22.767887019799197 24-25 22.606140449807217 25.86144886655868 28.992029783895727 22.540380899738373 26-27 22.225218836131134 26.221542255272006 28.78741494870213 22.76582395989473 28-29 22.310490539399012 26.65515901186194 28.61467434196176 22.41967610677729 30-31 22.443149144758497 26.156722559101492 28.584384951874075 22.815743344265936 32-33 22.11611296405725 26.162894456099718 28.675649709896057 23.04534286994698 34-35 22.79584488935471 26.341060374241565 28.49479371696184 22.36830101944188 36-37 22.8884302764828 25.881966849032022 28.4713780911721 22.758224783313075 38-39 22.454459348615057 26.225520513278784 28.51631684846075 22.80370328964541 40-41 22.873347950733823 26.161345858520257 28.49979115048197 22.465515040263956 42-43 22.581045768159353 26.115945607086605 28.332765686993362 22.970242937760677 44-45 22.549182428724333 26.39243421232016 28.521569970844755 22.536813388110748 46-47 23.034882867805404 26.586450182628663 27.837287974269387 22.54137897529655 48-49 22.8062995982566 26.669014796689282 27.76282862253174 22.761856982522378 50-51 22.890202696003588 26.88441152325392 27.762803835796806 22.462581944945686 52-53 23.242075185107335 26.563770320165297 27.687898322830367 22.506256171897 54-55 22.694908011469316 26.81077013376905 27.794630003450315 22.699691851311318 56-57 22.85768249977196 26.9721813516504 27.527652081490857 22.642484067086784 58-59 22.815817704470735 26.554177853746364 27.599211385241386 23.03079305654152 60-61 22.502587735126966 26.828269568631498 27.86147982756364 22.807662868677895 62-63 22.638840417051686 26.764270219035417 27.952769372320553 22.64411999159234 64-65 22.36165036029998 26.865251377150994 27.783822987019686 22.989275275529344 66-67 22.330727585220554 26.889109546020812 27.664835587027014 23.11532728173162 68-69 22.910417239866447 26.49107543433051 27.806819900009174 22.791687425793867 70-71 22.717389580053222 26.63066971774849 27.399331154744576 23.25260954745371 72-73 22.67427752190963 26.740891425840633 27.470737378647154 23.11409367360258 74-75 22.59960301565332 27.144176505347982 27.26434259630142 22.991877882697274 76-77 22.69738668496258 26.69033138873135 27.606672192456106 23.005609733849955 78-79 22.745125936442847 26.90062204789987 27.56208085631229 22.792171159344996 80-81 22.90592884121461 26.930875215086996 27.270057064049453 22.89313887964894 82-83 22.77592341322937 26.964330596982002 27.395678980826045 22.86406700896259 84-85 22.6255647603894 27.169427249244155 27.29485381731794 22.910154173048507 86-87 22.875707961015266 27.071284042796055 27.078447620490014 22.974560375698665 88-89 22.683059952171515 27.299316679291373 26.703840159269642 23.313783209267463 90-91 22.71172198398731 27.13707458542994 27.005701112956398 23.145502317626352 92-93 22.800325995137836 26.873158345594504 27.105657919262665 23.220857740004995 94-95 22.597278663697214 26.61947271165708 27.23460526495167 23.548643359694033 96-97 22.89205961116515 26.308864835817463 27.17714502138106 23.62193053163633 98-99 22.54487390492205 26.68314323560089 26.776068704863356 23.995914154613704 100-101 23.127486109513743 25.943556638680793 27.00532121625534 23.92363603555013 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 404.0 1 308.5 2 340.5 3 472.5 4 382.5 5 338.0 6 671.0 7 1347.0 8 2111.0 9 2441.5 10 2024.0 11 1532.5 12 1465.5 13 1622.5 14 1810.5 15 1933.0 16 2084.0 17 2288.0 18 2543.5 19 2922.0 20 3389.5 21 4046.0 22 5039.0 23 6213.0 24 7685.0 25 9648.0 26 11964.5 27 14758.5 28 17467.5 29 20022.0 30 22755.0 31 26695.0 32 32359.5 33 34381.0 34 34503.5 35 37044.5 36 40746.0 37 44367.0 38 47262.0 39 50448.0 40 53391.5 41 56486.0 42 59006.5 43 61564.5 44 64636.5 45 68374.5 46 70683.0 47 72745.0 48 75059.0 49 74638.0 50 73528.5 51 72392.0 52 71838.5 53 72364.0 54 72616.5 55 69204.5 56 64169.5 57 60068.5 58 55896.0 59 49754.0 60 41188.0 61 34450.0 62 29272.5 63 22859.0 64 16847.0 65 12786.5 66 9866.5 67 7685.0 68 6011.5 69 4671.0 70 3601.0 71 2607.5 72 1895.5 73 1476.0 74 1144.0 75 894.0 76 653.0 77 430.0 78 290.5 79 200.0 80 126.5 81 81.0 82 50.0 83 26.5 84 17.0 85 12.5 86 6.0 87 3.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01224464705672395 3 0.002230806143937561 4 0.0015367775658236534 5 0.11198646842566558 6 0.0 7 0.0 8 3.4701428905695397E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.478673493263957E-4 24-25 5.205214335854309E-4 26-27 0.0057753092393050195 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011005310310091969 36-37 5.948816383833497E-4 38-39 0.006766778636610602 40-41 0.010187348057314862 42-43 0.005304361275584868 44-45 0.0037180102398959355 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.957346986527914E-5 68-69 0.00114018980690142 70-71 0.0 72-73 7.436020479791871E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.957346986527914E-5 82-83 0.002230806143937561 84-85 0.004535972492673041 86-87 0.002949621456984109 88-89 0.0 90-91 0.00287526125218619 92-93 0.0 94-95 2.478673493263957E-5 96-97 9.914693973055828E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2017208.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.115490742827795 #Duplication Level Percentage of deduplicated Percentage of total 1 78.87402935735713 46.62676952324373 2 12.46064417700193 14.732341909904573 3 3.728020361173834 6.6115125945013595 4 1.65794185653893 3.9204018588949534 5 0.8605132779141658 2.543483235730763 6 0.5100196940155838 1.8090038700142863 7 0.34277285637214616 1.4184229932432173 8 0.270197694350023 1.2778295439265757 9 0.19954924233334817 1.0616806249105808 >10 1.0036534689583172 10.667768347419013 >50 0.05943887186888439 2.368528225786714 >100 0.028104016655263138 3.0963630330775933 >500 0.002851053535350781 1.189704203832955 >1k 0.002096362893640269 2.0543713049770407 >5k 1.6770903149122157E-4 0.6218187305366889 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6717 0.33298499708507995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5827 0.2888646089049815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3631 0.18000126908082856 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2988 0.14812552795745407 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2915 0.14450666465728867 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2492 0.12353708690427562 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2401 0.1190259011465352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.914693973055828E-5 2 0.0 0.0 0.0 0.0 9.914693973055828E-5 3 0.0 0.0 0.0 0.0 9.914693973055828E-5 4 0.0 0.0 0.0 0.0 9.914693973055828E-5 5 0.0 0.0 0.0 0.0 9.914693973055828E-5 6 9.914693973055828E-5 0.0 0.0 0.0 9.914693973055828E-5 7 9.914693973055828E-5 0.0 0.0 4.957346986527914E-5 9.914693973055828E-5 8 9.914693973055828E-5 0.0 0.0 4.957346986527914E-5 9.914693973055828E-5 9 9.914693973055828E-5 0.0 0.0 4.957346986527914E-5 1.4872040959583742E-4 10-11 9.914693973055828E-5 0.0 0.0 4.957346986527914E-5 1.4872040959583742E-4 12-13 9.914693973055828E-5 0.0 0.0 4.957346986527914E-5 2.478673493263957E-4 14-15 1.4872040959583742E-4 0.0 0.0 4.957346986527914E-5 3.4701428905695397E-4 16-17 2.478673493263957E-4 0.0 0.0 9.914693973055828E-5 4.2137449385487265E-4 18-19 2.478673493263957E-4 0.0 0.0 9.914693973055828E-5 4.957346986527914E-4 20-21 2.478673493263957E-4 0.0 0.0 1.7350714452847699E-4 5.948816383833497E-4 22-23 2.478673493263957E-4 0.0 0.0 2.478673493263957E-4 6.692418431812684E-4 24-25 2.478673493263957E-4 0.0 0.0 5.453081685180706E-4 7.43602047979187E-4 26-27 2.478673493263957E-4 0.0 0.0 7.931755178444662E-4 7.931755178444662E-4 28-29 2.726540842590353E-4 0.0 0.0 0.0025034602281965965 7.931755178444662E-4 30-31 2.9744081919167483E-4 0.0 0.0 0.008154835792838419 8.179622527771058E-4 32-33 2.9744081919167483E-4 0.0 0.0 0.020473843054360286 8.923224575750245E-4 34-35 2.9744081919167483E-4 0.0 0.0 0.0402784442655393 8.923224575750245E-4 36-37 2.9744081919167483E-4 0.0 0.0 0.07505423337603262 9.17109192507664E-4 38-39 2.9744081919167483E-4 0.0 0.0 0.1291636757339848 9.418959274403036E-4 40-41 2.9744081919167483E-4 0.0 0.0 0.18659454057291067 9.418959274403036E-4 42-43 2.9744081919167483E-4 0.0 0.0 0.24628099829070677 9.418959274403036E-4 44-45 2.9744081919167483E-4 0.0 0.0 0.3182616765350921 9.666826623729431E-4 46-47 3.4701428905695397E-4 0.0 0.0 0.4012228783546367 0.0010162561322382222 48-49 3.4701428905695397E-4 0.0 0.0 0.49114915269025305 0.001090616337036141 50-51 3.4701428905695397E-4 0.0 0.0 0.590667893444801 0.001090616337036141 52-53 3.4701428905695397E-4 0.0 0.0 0.6854027943573493 0.0011401898069014202 54-55 3.4701428905695397E-4 0.0 0.0 0.7883421045326016 0.0011897632767666993 56-57 3.4701428905695397E-4 0.0 0.0 0.9061534556674373 0.0011897632767666993 58-59 3.4701428905695397E-4 0.0 0.0 1.0292939548127906 0.0011897632767666993 60-61 3.4701428905695397E-4 0.0 0.0 1.1607132234256459 0.001214550011699339 62-63 3.965877589222331E-4 0.0 0.0 1.291240169580926 0.0012393367466319784 64-65 3.965877589222331E-4 0.0 0.0 1.4222132769649933 0.0012889102164972575 66-67 4.957346986527914E-4 0.0 0.0 1.578543214185151 0.0012889102164972575 68-69 4.957346986527914E-4 0.0 0.0 1.7509597423765917 0.0012889102164972575 70-71 4.957346986527914E-4 0.0 0.0 1.9279122430607059 0.0013384836863625368 72-73 5.948816383833497E-4 0.0 0.0 2.105186971298944 0.001437630626093095 74-75 6.196683733159892E-4 0.0 0.0 2.2839241168982074 0.001487204095958374 76-77 6.444551082486288E-4 0.0 0.0 2.475575151397377 0.001487204095958374 78-79 6.444551082486288E-4 0.0 0.0 2.690203489179103 0.0015367775658236534 80-81 6.940285781139079E-4 0.0 0.0 2.9222569016184745 0.0015367775658236534 82-83 6.940285781139079E-4 0.0 0.0 3.1536162854797323 0.0015863510356889324 84-85 6.940285781139079E-4 0.0 0.0 3.385471404039643 0.001611137770621572 86-87 7.188153130465475E-4 0.0 0.0 3.619854769562683 0.0016854979754194906 88-89 7.683887829118266E-4 0.0 0.0 3.867127237250695 0.0017598581802174096 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4170 0.0 41.282204 1 GTATCAA 6530 0.0 34.22749 1 TATCAAC 7870 0.0 28.097559 2 ATCAACG 7830 0.0 28.055416 3 TCAACGC 7920 0.0 27.736605 4 CAACGCA 8005 0.0 27.323292 5 AACGCAG 8325 0.0 26.357168 6 GTACATG 5940 0.0 23.216776 1 ACGCAGA 9690 0.0 22.546288 7 CGCAGAG 9755 0.0 22.396057 8 TACATGG 5820 0.0 22.143013 2 ACATGGG 5745 0.0 21.849947 3 GTGTAGC 1115 0.0 21.751324 1 TAAGGTG 1180 0.0 21.356514 5 GAGTACT 6825 0.0 20.598238 12-13 GTACTTT 6930 0.0 19.97774 14-15 GCAGAGT 10810 0.0 19.902771 9 GTAAGGT 1140 0.0 19.603313 4 ACCGTCC 245 1.7598904E-7 19.385408 8 AGAGTAC 9215 0.0 19.069855 10-11 >>END_MODULE