FastQCFastQC Report
Thu 26 May 2016
SRR937910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020111
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72110.35696058285906074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55520.274836382753225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.17157473029947365No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30860.1527638827767385No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29860.1478136597444398No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26290.13014136351913336No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24950.12350806465585307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.041.616061
GTATCAA65200.036.4403231
ATCAACG78550.029.7066543
TCAACGC79250.029.4442624
TATCAAC80550.029.2050572
CAACGCA80450.028.9459925
AACGCAG83100.028.0766356
ACGCAGA95250.024.5365857
CGCAGAG96250.024.2323068
GGACCGA3750.022.8089796
GTACATG60600.022.2012251
TACATGG57500.022.0679442
GAGTACT66100.021.12885512-13
GCAGAGT108400.021.0336769
ACATGGG56050.021.027833
GTACTTT67800.020.53155514-15
GTGTAGC12650.020.3659041
AGAGTAC89250.020.25064810-11
TAAGGTG12100.019.6382625
CAGAGTA101150.019.32397310-11
Produced by FastQC (version 0.11.5)