FastQCFastQC Report
Thu 26 May 2016
SRR937910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020111
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69820.3456245721150966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58960.29186514998433255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.1880589729970284No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28870.14291293894246404No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27700.13712117799467455No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25220.12484462487457372No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24230.119943904072598No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20560.10177658554406169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.045.416331
GTATCAA64000.036.744371
ATCAACG78800.029.3557453
CAACGCA80200.028.901815
TCAACGC80550.028.8351964
TATCAAC82100.028.354272
AACGCAG84150.027.6573646
TAAGGTG12400.023.7492355
ACGCAGA98600.023.6523027
GTAAGGT11900.023.1505134
CGCAGAG99950.023.1427528
GTACATG59900.021.4142321
GGTAAGG13050.021.1109523
GAGTACT71600.021.02882812-13
GTACTTT72400.020.73086214-15
TACATGG59900.020.621112
GAGTACA45950.020.5746821
GCAGAGT110950.020.4630079
AGAGTAC93250.019.99215510-11
ACATGGG59900.019.4280663
Produced by FastQC (version 0.11.5)