FastQCFastQC Report
Thu 26 May 2016
SRR937909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018445
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73250.3629031259211918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.2764999789441872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36570.18117907597184962No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30770.15244408443133203No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30410.15066053323226541No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26650.13203233181979196No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25520.12643396277827734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.041.0249061
GTATCAA68450.035.648391
ATCAACG83700.029.0676443
TCAACGC84050.028.9466044
CAACGCA84500.028.7362145
TATCAAC87450.027.9298532
AACGCAG87450.027.9298536
GTACATG62350.025.1205941
TACATGG61400.024.4559232
ACGCAGA100150.024.3016197
CGCAGAG100300.024.1698888
ACATGGG60550.023.543583
GAGTACT68200.021.70907612-13
GTAAGGT10150.021.5356084
GCAGAGT113400.021.3353319
GTACTTT70150.020.92403214-15
AGAGTAC92450.020.87490510-11
GGACCGT2308.9914465E-820.6603436
AGTACTT71450.020.3557412-13
GAGTACA44950.020.1844271
Produced by FastQC (version 0.11.5)