##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937909_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018445 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19376103881949 33.0 31.0 34.0 31.0 34.0 2 32.39536276688243 34.0 31.0 34.0 31.0 34.0 3 32.47817057190065 34.0 31.0 34.0 31.0 34.0 4 35.986569859471025 37.0 35.0 37.0 35.0 37.0 5 36.00628850426938 37.0 37.0 37.0 35.0 37.0 6 36.03771665812048 37.0 37.0 37.0 35.0 37.0 7 35.989318510041144 37.0 37.0 37.0 35.0 37.0 8 35.974759777947874 37.0 37.0 37.0 35.0 37.0 9 37.694624822573815 39.0 38.0 39.0 35.0 39.0 10-11 37.626176091000744 39.0 38.0 39.0 35.0 39.0 12-13 37.5429404814102 39.0 38.0 39.0 35.0 39.0 14-15 38.87245850147019 40.0 38.0 41.0 36.0 41.0 16-17 38.79314224563959 40.0 38.0 41.0 35.0 41.0 18-19 38.71934112646121 40.0 38.0 41.0 35.0 41.0 20-21 38.6060806710116 40.0 38.0 41.0 34.5 41.0 22-23 38.459687036307656 40.0 38.0 41.0 34.0 41.0 24-25 38.42011548494014 40.0 38.0 41.0 34.0 41.0 26-27 38.450678864175146 40.0 38.0 41.0 34.0 41.0 28-29 38.37104454171404 40.0 38.0 41.0 34.0 41.0 30-31 38.20173945784998 40.0 38.0 41.0 34.0 41.0 32-33 38.001751843622195 40.0 38.0 41.0 33.5 41.0 34-35 37.81129879684609 40.0 38.0 41.0 33.0 41.0 36-37 37.61340091010654 40.0 37.0 41.0 33.0 41.0 38-39 37.379540190592266 40.0 37.0 41.0 32.5 41.0 40-41 37.165681997775515 40.0 37.0 41.0 31.5 41.0 42-43 36.89456561858262 39.5 36.0 41.0 31.0 41.0 44-45 36.69577199279644 39.0 36.0 41.0 31.0 41.0 46-47 36.599856572757744 39.0 36.0 41.0 30.5 41.0 48-49 36.35803304028596 39.0 36.0 41.0 30.0 41.0 50-51 35.72228076563889 38.5 35.0 40.5 29.0 40.5 52-53 35.808124818858076 39.0 35.0 40.0 29.5 41.0 54-55 35.95523484662698 39.0 35.0 40.5 29.5 41.0 56-57 35.743043035604146 39.0 35.0 41.0 28.5 41.0 58-59 35.471972236053006 38.5 35.0 40.0 28.0 41.0 60-61 35.095552021481886 38.0 35.0 40.0 27.5 41.0 62-63 34.677907002667894 37.5 34.0 40.0 26.5 41.0 64-65 34.2468791569748 37.0 34.0 40.0 26.0 41.0 66-67 33.81925318747848 36.0 34.0 39.0 25.5 41.0 68-69 33.33159486634513 36.0 33.0 39.0 24.5 40.5 70-71 32.84561283562346 35.0 33.0 38.5 23.0 40.0 72-73 32.355664385207426 35.0 32.0 37.5 21.5 40.0 74-75 31.836133261000423 35.0 32.0 37.0 20.0 39.0 76-77 31.312969389802547 35.0 31.0 36.5 18.0 39.0 78-79 30.756794463064388 34.5 31.0 36.0 14.0 38.5 80-81 30.238013668938216 34.0 30.0 35.5 7.5 37.0 82-83 29.7048604247329 34.0 29.5 35.0 6.0 37.0 84-85 29.224060601106295 34.0 29.0 35.0 2.0 36.0 86-87 28.742260750230994 34.0 29.0 35.0 2.0 36.0 88-89 28.186987259994698 33.5 28.0 35.0 2.0 35.5 90-91 27.580899900666108 33.0 26.5 35.0 2.0 35.0 92-93 26.97494952797822 33.0 25.5 35.0 2.0 35.0 94-95 26.374046852899138 32.5 25.0 35.0 2.0 35.0 96-97 25.527577417269235 32.0 22.0 34.5 2.0 35.0 98-99 24.42980413139818 32.0 10.0 34.0 2.0 35.0 100-101 21.423631062525857 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32019237850820303 1101 2 0.23292047803204952 1101 3 0.18659734632171165 1101 4 0.07175503327655264 1101 5 0.12262720154514994 1101 6 0.18541111269627208 1101 7 0.1468431057042281 1101 8 0.18604880553947822 1101 9 0.09599101989930148 1101 10-11 0.16177593506951382 1101 12-13 0.07814396355680486 1101 14-15 0.228953658863162 1101 16-17 0.30505062453321585 1101 18-19 0.20048825426714245 1101 20-21 0.3518600462726411 1101 22-23 0.29960588183050163 1101 24-25 0.25510950411955235 1101 26-27 0.2921907357782203 1101 28-29 0.22438221958420002 1101 30-31 0.2658233941732817 1101 32-33 0.34666254453458123 1101 34-35 0.3507071352074931 1101 36-37 0.3220457928035785 1101 38-39 0.44338316039700487 1101 40-41 0.39982146193244716 1101 42-43 0.46372679956915874 1101 44-45 0.3498818027928863 1101 46-47 0.4962388101141002 1101 48-49 0.38240349371983484 1101 50-51 0.5336657810243182 1101 52-53 0.49460384083223374 1101 54-55 0.4889397157588533 1101 56-57 0.4603651055453213 1101 58-59 0.531554434971973 1101 60-61 0.40492814928917653 1101 62-63 0.48762002255040926 1101 64-65 0.37607608690782257 1101 66-67 0.3029258844115361 1101 68-69 0.36686809968125544 1101 70-71 0.3428180563198211 1101 72-73 0.48524115395025547 1101 74-75 0.3660852022640526 1101 76-77 0.4515949774609105 1101 78-79 0.6300362528782557 1101 80-81 0.6505600213447664 1101 82-83 0.5184298586481795 1101 84-85 0.6753591425354308 1101 86-87 0.7270858232371324 1101 88-89 0.6387850371284074 1101 90-91 0.6546488618938362 1101 92-93 0.6646984050856872 1101 94-95 0.7314630695192541 1101 96-97 0.9335467346800286 1101 98-99 1.0198349258474693 1101 100-101 0.928999509424802 1102 1 0.36393230473164095 1102 2 0.29468266668603604 1102 3 0.2475926122271943 1102 4 0.19279598807707998 1102 5 0.14640630978144742 1102 6 0.23321699596471746 1102 7 0.12834324037304867 1102 8 0.08591740141790893 1102 9 0.3228858890031816 1102 10-11 0.29619342980166863 1102 12-13 0.18886720407419233 1102 14-15 0.27854035082562234 1102 16-17 0.311168275830191 1102 18-19 0.2703592789688045 1102 20-21 0.3295297887920441 1102 22-23 0.3633938722987011 1102 24-25 0.26097414126939356 1102 26-27 0.30909624611113173 1102 28-29 0.34719399197503265 1102 30-31 0.3526767622024778 1102 32-33 0.40151497030419847 1102 34-35 0.37734735781491935 1102 36-37 0.460498567670804 1102 38-39 0.523345340551522 1102 40-41 0.49618790859782536 1102 42-43 0.5893730902945151 1102 44-45 0.411657641264604 1102 46-47 0.559110855596991 1102 48-49 0.5847785057161943 1102 50-51 0.6552031951746997 1102 52-53 0.6381781117420005 1102 54-55 0.7276677103529607 1102 56-57 0.6663712504962547 1102 58-59 0.6239372456580057 1102 60-61 0.6326293426772622 1102 62-63 0.5725206223262163 1102 64-65 0.42855316295362655 1102 66-67 0.47233799379807806 1102 68-69 0.4816972616584323 1102 70-71 0.4564436421010498 1102 72-73 0.7189050661681335 1102 74-75 0.579022499504454 1102 76-77 0.5187671376866909 1102 78-79 0.32754714529207973 1102 80-81 0.6880097738327642 1102 82-83 0.915570742497799 1102 84-85 0.7563912151040562 1102 86-87 0.715919568738677 1102 88-89 0.7482983690601728 1102 90-91 0.7771002509527598 1102 92-93 0.9055031420508861 1102 94-95 0.8349392418626991 1102 96-97 0.9692329870057748 1102 98-99 1.1233600686710012 1102 100-101 1.0175541794777523 1103 1 0.3152481573945636 1103 2 0.21225538275320588 1103 3 0.202342168006858 1103 4 0.1265039072049703 1103 5 0.06920001545946519 1103 6 0.0730103334601182 1103 7 0.041789909921149615 1103 8 0.0959439745982067 1103 9 0.1594296146911205 1103 10-11 0.09951547753734502 1103 12-13 0.010347458280278943 1103 14-15 0.09864632338801016 1103 16-17 0.05270887996984186 1103 18-19 0.18945234913160647 1103 20-21 0.27291650860507843 1103 22-23 0.1772043222818951 1103 24-25 0.3179087808506367 1103 26-27 0.20419261390956223 1103 28-29 0.15379246481757036 1103 30-31 0.20252108498658572 1103 32-33 0.24971087547316273 1103 34-35 0.18704816941747282 1103 36-37 0.26372456776685027 1103 38-39 0.19690102417060018 1103 40-41 0.2321741577774148 1103 42-43 0.07211973262911897 1103 44-45 0.07735942935149609 1103 46-47 0.11843668120648942 1103 48-49 0.07955871670084491 1103 50-51 0.06508129471206558 1103 52-53 0.12043931329797175 1103 54-55 0.007814263043094627 1103 56-57 -0.05049751641213618 1103 58-59 0.009701784869456276 1103 60-61 -0.004543094851754859 1103 62-63 0.01291465776780143 1103 64-65 0.09783832839568873 1103 66-67 0.14378142974230457 1103 68-69 0.2367473665104427 1103 70-71 0.2856401235558437 1103 72-73 0.28407499045057705 1103 74-75 0.19944382848363063 1103 76-77 0.24833300235556877 1103 78-79 0.18440734795260028 1103 80-81 0.28853083227775045 1103 82-83 0.2268147195103971 1103 84-85 0.26120360204797777 1103 86-87 0.2747283378881704 1103 88-89 0.3638260708002363 1103 90-91 0.5130752945743957 1103 92-93 0.6410199583917908 1103 94-95 0.7941265987917383 1103 96-97 0.9161562609496485 1103 98-99 0.7943528811059686 1103 100-101 0.6044824959227064 1104 1 0.2555583388551881 1104 2 0.18328872502549132 1104 3 0.12446159233867604 1104 4 0.1068918575044222 1104 5 0.11255390837087731 1104 6 0.09918803972632162 1104 7 0.10317365891551589 1104 8 0.11328106228927481 1104 9 0.10555905064356352 1104 10-11 0.1565526635568375 1104 12-13 0.10461759325837505 1104 14-15 0.16306484488617912 1104 16-17 0.19815448647739942 1104 18-19 0.3199185152172461 1104 20-21 0.24008599104004702 1104 22-23 0.20535977057009092 1104 24-25 0.23276978022490624 1104 26-27 0.21906925644106678 1104 28-29 0.3520180838396456 1104 30-31 0.2341403752341904 1104 32-33 0.2642724201231985 1104 34-35 0.2748867320347941 1104 36-37 0.2837992079742335 1104 38-39 0.1978798388662284 1104 40-41 0.2812959062354494 1104 42-43 0.3137975729795244 1104 44-45 0.29532000859526164 1104 46-47 0.342082430737193 1104 48-49 0.2863813679925258 1104 50-51 0.30698931294752896 1104 52-53 0.3169955839692804 1104 54-55 0.4284431179639867 1104 56-57 0.4280467760702109 1104 58-59 0.3311262060726392 1104 60-61 0.4264996333148616 1104 62-63 0.40806751376135963 1104 64-65 0.40670352907504537 1104 66-67 0.3716620054104922 1104 68-69 0.47739052374651436 1104 70-71 0.6360714277482131 1104 72-73 0.6665233680317044 1104 74-75 0.6104521417043287 1104 76-77 0.46874508781190016 1104 78-79 0.5442580675378323 1104 80-81 0.45909934930644525 1104 82-83 0.5265778374151076 1104 84-85 0.5424563954689141 1104 86-87 0.41024947849781057 1104 88-89 0.6102996562118754 1104 90-91 0.6498300098689356 1104 92-93 0.6528193684212908 1104 94-95 0.7647018200306732 1104 96-97 0.8814016350445826 1104 98-99 0.9333758541997383 1104 100-101 0.7010477055410114 1105 1 0.27034477261956624 1105 2 0.23794235230608507 1105 3 0.24564610610394766 1105 4 0.17908679744834188 1105 5 0.12545442834725407 1105 6 0.11147558512080735 1105 7 0.21125776968857934 1105 8 0.17939114242482646 1105 9 0.15429193259195273 1105 10-11 0.23322813910307616 1105 12-13 0.2013725450970938 1105 14-15 0.2051507398781638 1105 16-17 0.1326552768919882 1105 18-19 0.08440652714570263 1105 20-21 0.14348849091030047 1105 22-23 0.09203486739061617 1105 24-25 0.263222834367248 1105 26-27 0.282457070169734 1105 28-29 0.2995452854633953 1105 30-31 0.33469626042138145 1105 32-33 0.2791356510462961 1105 34-35 0.2899358190954189 1105 36-37 0.3495835138611696 1105 38-39 0.3468621187108596 1105 40-41 0.3186368589690787 1105 42-43 0.40817356625784385 1105 44-45 0.45859006668056423 1105 46-47 0.46250107694705633 1105 48-49 0.391369045379939 1105 50-51 0.2951937603095587 1105 52-53 0.3837698367925455 1105 54-55 0.38355851007835895 1105 56-57 0.3907959460296837 1105 58-59 0.23736248603230337 1105 60-61 0.34527138374303945 1105 62-63 0.25684864817112185 1105 64-65 0.27216612850808275 1105 66-67 0.20736447848940287 1105 68-69 0.11119116253055239 1105 70-71 0.2743168335948951 1105 72-73 0.2688427824936923 1105 74-75 0.0911627293323427 1105 76-77 -0.021941064994106085 1105 78-79 0.17005987896889607 1105 80-81 0.2597991502650423 1105 82-83 0.2678657433003657 1105 84-85 0.400839980093771 1105 86-87 0.5010359936180819 1105 88-89 0.5133325478388073 1105 90-91 0.2389304942170405 1105 92-93 0.33851559953935606 1105 94-95 0.7212980998220111 1105 96-97 0.7727413702451713 1105 98-99 0.4749858873452517 1105 100-101 0.3427917655087249 1106 1 0.08208878857535495 1106 2 0.03048219590617407 1106 3 0.08503161608458498 1106 4 0.1284182387048176 1106 5 0.09187074370427695 1106 6 0.14938893606549186 1106 7 0.16921999928548814 1106 8 0.12727611448203646 1106 9 0.16875367954162357 1106 10-11 -0.26347375254335503 1106 12-13 -0.18025292381604885 1106 14-15 -0.060541072245314353 1106 16-17 0.020775924883004393 1106 18-19 0.15716460749417394 1106 20-21 0.062490066961387924 1106 22-23 -0.06864543301283277 1106 24-25 -0.013691403941550107 1106 26-27 0.053996151909863954 1106 28-29 0.008063618469378753 1106 30-31 0.14754625329664606 1106 32-33 0.08697080203751995 1106 34-35 -0.003346968238268744 1106 36-37 -0.09904089053220133 1106 38-39 0.04524078191960257 1106 40-41 0.15996360537391752 1106 42-43 0.1312277253721703 1106 44-45 0.20159836467905023 1106 46-47 0.3399359762050693 1106 48-49 0.3070488394625315 1106 50-51 0.5610482294236405 1106 52-53 0.36601621138796503 1106 54-55 -0.10222021706046291 1106 56-57 0.043716058113489 1106 58-59 0.30875508586415634 1106 60-61 0.12052235237901243 1106 62-63 0.05497761704133097 1106 64-65 0.1571706944574487 1106 66-67 -0.06511361888595246 1106 68-69 -0.47348754573733487 1106 70-71 -0.06062709844982095 1106 72-73 0.010590262273140638 1106 74-75 0.16941558030387682 1106 76-77 0.2694794357221042 1106 78-79 0.3078340879586605 1106 80-81 0.16140175205195817 1106 82-83 0.171117682441281 1106 84-85 0.17599648081179353 1106 86-87 0.2675939881978415 1106 88-89 0.1432972956740315 1106 90-91 0.12423873940863572 1106 92-93 0.34863204880521437 1106 94-95 0.1776412353911745 1106 96-97 0.08251662495174727 1106 98-99 0.05226189020602234 1106 100-101 -0.10408928669624018 1107 1 0.24508514993513586 1107 2 0.13546537941597592 1107 3 0.17258555111668983 1107 4 0.15980480825831478 1107 5 0.16880471767002803 1107 6 0.10185649634536986 1107 7 0.11736699299008535 1107 8 0.15239188788650893 1107 9 0.12935713559583917 1107 10-11 0.1746607177477344 1107 12-13 0.07404187099749748 1107 14-15 0.1615964387811175 1107 16-17 0.2554331914889403 1107 18-19 0.27262387358977236 1107 20-21 0.20592704674590578 1107 22-23 0.38744269953981814 1107 24-25 0.3529363482886225 1107 26-27 0.32198888748759913 1107 28-29 0.23355190190599018 1107 30-31 0.3107411403564697 1107 32-33 0.33512504508928487 1107 34-35 0.2756996916194794 1107 36-37 0.23342259503817075 1107 38-39 0.1887358451585115 1107 40-41 0.1407096053193797 1107 42-43 0.23125741000784217 1107 44-45 0.23285446815273048 1107 46-47 0.16807933858530077 1107 48-49 0.11001180505295594 1107 50-51 0.25106714201713487 1107 52-53 0.20417276225943226 1107 54-55 0.206252695764924 1107 56-57 0.17743525476167576 1107 58-59 0.17625952137833423 1107 60-61 0.2063018600485762 1107 62-63 0.1817504094211344 1107 64-65 0.16424024895069778 1107 66-67 0.23142802129769535 1107 68-69 0.18460530842376244 1107 70-71 0.23164308006369083 1107 72-73 0.3112245690659563 1107 74-75 0.356562243908229 1107 76-77 0.3339436457838012 1107 78-79 0.2866663267588123 1107 80-81 0.286981838383479 1107 82-83 0.157280851279463 1107 84-85 0.19336432044648078 1107 86-87 0.46634071573833324 1107 88-89 0.2764065052047755 1107 90-91 0.3033729900023836 1107 92-93 0.4847715741303773 1107 94-95 0.7070176075296501 1107 96-97 0.6310429124812487 1107 98-99 0.5170627626106388 1107 100-101 0.4109808202549061 1108 1 0.2588002193676786 1108 2 0.19664438556326047 1108 3 0.16343808962909634 1108 4 0.22324006578323718 1108 5 0.22170723046667717 1108 6 0.18824918817265512 1108 7 0.17565297667895408 1108 8 0.19827461179725958 1108 9 0.18391127338330904 1108 10-11 0.17336344974058449 1108 12-13 0.11770201892431231 1108 14-15 0.31043479076858205 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88-89 -0.6479246513412491 2316 90-91 -0.49531006882872575 2316 92-93 -0.6481550958809521 2316 94-95 -0.7460944392978632 2316 96-97 -0.9607755796589217 2316 98-99 -0.754104606738732 2316 100-101 -0.6474950952644782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4171.0 3 933.0 4 975.0 5 1090.0 6 1261.0 7 1508.0 8 1844.0 9 2289.0 10 3000.0 11 3849.0 12 5040.0 13 6497.0 14 7937.0 15 9967.0 16 12161.0 17 16542.0 18 14685.0 19 12230.0 20 11949.0 21 12312.0 22 13058.0 23 14570.0 24 16223.0 25 18577.0 26 21342.0 27 25383.0 28 29988.0 29 36671.0 30 45365.0 31 56693.0 32 74241.0 33 99285.0 34 139818.0 35 208629.0 36 315953.0 37 426715.0 38 309746.0 39 35941.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.257117785342295 17.987473912279558 13.570492467474033 24.184915834904114 2 15.751453538803167 19.065075911156935 40.09680345379655 25.086667096243353 3 19.91136865533659 22.69046692558784 28.859267998284878 28.538896420790692 4 12.880528624140561 15.51318908108971 36.04422254842326 35.56205974634648 5 14.047157706014998 36.82800070603681 34.07708494059176 15.047756647356428 6 34.00287847708526 35.01535452283936 16.50442583210008 14.477341167975306 7 29.777577270394946 31.853770626715022 19.779485946443046 18.589166156446986 8 26.98900741579349 34.87197458575202 19.003385955842298 19.135632042612187 9 26.251708175898624 16.527063404614516 19.741284151745994 37.47994426774087 10-11 25.665568572000147 25.9095721645025 26.60263855068361 21.822220712813746 12-13 26.318609882317702 23.05826187678187 27.34431589056987 23.278812350330565 14-15 22.713227805920567 24.77896569851003 25.298076896862554 27.209729598706843 16-17 22.677668293033555 27.96843765917183 26.286616491781622 23.067277556012993 18-19 22.785993568095773 26.467347861918544 28.36231821195541 22.384340358030276 20-21 22.900794203637204 25.68987733696118 28.872443032659078 22.536885426742536 22-23 22.63430108507702 25.64161226739851 28.758804352999036 22.965282294525437 24-25 22.542515084078115 25.86678904505191 28.853778655781536 22.736917215088443 26-27 22.210021195909345 26.141266236832493 28.697664930127363 22.95104763713079 28-29 22.34735925739742 26.63909272716276 28.476091183379094 22.53745683206073 30-31 22.4644422646789 26.126200492653457 28.42065574916468 22.98870149350297 32-33 22.090365089437988 26.171865704200215 28.540683377622656 23.19708582873914 34-35 22.79911155841677 26.344697486136692 28.361440048784964 22.494750906661576 36-37 22.85366333094625 25.85779474598627 28.37987403236965 22.908667890697835 38-39 22.442327154931867 26.225049239867293 28.358595095144214 22.97402851005663 40-41 22.864629825223123 26.126077455354157 28.43138955621057 22.577903163212152 42-43 22.53498613837439 26.107189136906566 28.214622125419524 23.143202599299524 44-45 22.46756142702467 26.4108580333937 28.477858521489857 22.643722018091765 46-47 23.030094745746503 26.58669020237105 27.742405944318104 22.640809107564348 48-49 22.713639333587555 26.669506196791332 27.737650212010816 22.8792042576103 50-51 22.76520927291531 26.91664812486651 27.74991414609683 22.568228456121346 52-53 23.092348083601593 26.53759851572287 27.758118131650306 22.61193526902523 54-55 22.53527757356995 26.78531120901524 27.91808858498469 22.76132263243012 56-57 22.738362863444102 26.975346741041793 27.583309650274522 22.702980745239582 58-59 22.70051726329388 26.561843030151515 27.664778103536403 23.0728616030182 60-61 22.355860958888496 26.817850236733836 27.984174464462175 22.842114339915494 62-63 22.484330410920077 26.692982095366837 28.20114752579307 22.621539967920018 64-65 22.245267334729917 26.85980954127122 27.95997071390433 22.934952410094535 66-67 22.198627842722196 26.896527290529477 27.84978316624846 23.05506170049987 68-69 22.749818887438668 26.462923706767118 28.02078904623301 22.766468359561205 70-71 22.57465442127645 26.622225085868074 27.63080941500672 23.172311077848764 72-73 22.49475019242273 26.776199352428737 27.676906248869376 23.052144206279152 74-75 22.381660715909646 27.1505178509082 27.5917634485498 22.876057984632354 76-77 22.586751476457454 26.760624101216745 27.60472466385135 23.047899758474454 78-79 22.677957098736528 26.97316230702186 27.556364209340252 22.79251638490136 80-81 22.872308929170902 26.934034311424053 27.299867575592785 22.893789183812263 82-83 22.714404782433753 27.022990487856607 27.4951785186903 22.76742621101934 84-85 22.601714080713222 27.218166718530423 27.35762902679143 22.822490173964923 86-87 22.85052543853847 27.132442618454995 27.17849699234467 22.838534950661867 88-89 22.682637609364487 27.280574041972667 26.90376916395411 23.133019184708736 90-91 22.743726421956907 27.30756561948617 26.99046951983759 22.958238438719334 92-93 22.894627028109305 26.88517895533972 27.295930251575257 22.924263764975723 94-95 22.686452192387403 26.656280190961784 27.476284681964163 23.180982934686647 96-97 23.042235482404816 26.32660890408209 27.414353610706748 23.216802002806347 98-99 22.749072123359397 26.68778394462426 27.007368002144933 23.55577592987141 100-101 23.227277452991448 26.03869662106149 27.258818546409074 23.47520737953799 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 451.0 1 335.0 2 369.0 3 520.0 4 409.0 5 377.0 6 738.5 7 1450.0 8 2363.5 9 2707.5 10 2185.0 11 1698.0 12 1656.5 13 1748.0 14 1776.0 15 1891.5 16 2062.5 17 2222.0 18 2478.5 19 2818.0 20 3202.0 21 3844.0 22 4750.5 23 5778.0 24 7229.0 25 9276.5 26 11719.0 27 14333.0 28 16696.0 29 19019.5 30 21838.0 31 25772.5 32 31825.5 33 34336.0 34 34243.0 35 36906.0 36 40886.0 37 44534.5 38 47582.5 39 50515.5 40 53308.5 41 57019.5 42 59507.0 43 61770.0 44 64947.5 45 69648.5 46 72378.5 47 73737.0 48 76056.5 49 75719.0 50 74212.5 51 72871.0 52 73014.5 53 73948.0 54 74329.0 55 70509.0 56 64822.5 57 60464.5 58 56503.5 59 50139.0 60 40347.0 61 33429.0 62 28599.5 63 21581.0 64 15189.5 65 11698.0 66 9070.0 67 7053.0 68 5578.0 69 4296.5 70 3288.5 71 2330.5 72 1679.5 73 1401.0 74 1089.0 75 788.0 76 567.5 77 410.0 78 259.5 79 169.5 80 136.5 81 92.5 82 55.5 83 29.5 84 17.5 85 10.0 86 8.5 87 12.5 88 9.5 89 4.5 90 5.0 91 3.0 92 1.5 93 2.0 94 2.5 95 2.5 96 1.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4636242255795922 2 0.04731364986412808 3 0.05449739774925748 4 0.06361332610004236 5 0.07694041700417896 6 0.10409002970108178 7 0.06638773907636819 8 0.04924583032978357 9 0.04666958970890958 10-11 0.07265493981753281 12-13 0.1053533784670873 14-15 0.052243187205992726 16-17 0.057841556247507364 18-19 0.05734612535887775 20-21 0.07213473738447171 22-23 0.06247383505619424 24-25 0.08665086242131938 26-27 0.06492621795491084 28-29 0.06554550656569785 30-31 0.06978144066348105 32-33 0.06960803985246068 34-35 0.07084661707403471 36-37 0.06636296753193671 38-39 0.03819772151334319 40-41 0.04181436700033937 42-43 0.040848276767511627 44-45 0.019817235545184535 46-47 0.025663320031013973 48-49 0.025044031420226955 50-51 0.023879768831947364 52-53 0.02442474280943994 54-55 0.02400362655410477 56-57 0.02360728184320108 58-59 0.020832868866875243 60-61 0.028090931385299077 62-63 0.018033684346117927 64-65 0.020931955044601165 66-67 0.015308814458655054 68-69 0.017984141257254966 70-71 0.04037761742331349 72-73 0.037256402824946924 74-75 0.03143508988354897 76-77 0.021749416010840027 78-79 0.01367389252617733 80-81 0.015482215269675418 82-83 0.019470433923143807 84-85 0.01669602094681797 86-87 0.0088434413620386 88-89 0.0407491905897857 90-91 0.05328359207211492 92-93 0.03363975733795075 94-95 0.053407449794272324 96-97 0.04339974584395413 98-99 0.03299569718273225 100-101 0.1664400070351186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.37398477652567 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35605042773415 47.11684930021491 2 12.010563485004615 14.262300270323184 3 3.6554639604905326 6.511183846239094 4 1.6690765229783113 3.963988962646826 5 0.8812646982572497 2.6162098389207715 6 0.5523943724295999 1.9678713035564108 7 0.35211022280504467 1.4634330905940052 8 0.2570996646240012 1.221202525874824 9 0.1849691345854619 0.9884119122903909 >10 0.9892345038454909 10.535516194391494 >50 0.05971048122545758 2.4168254752127387 >100 0.02688767163939814 2.9727591378935787 >500 0.0029212887632675704 1.2253397028580526 >1k 0.0020866348309053944 2.0985292832333107 >5k 1.6693078647243153E-4 0.6395791557504631 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7325 0.3629031259211918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5581 0.2764999789441872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3657 0.18117907597184962 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3077 0.15244408443133203 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3041 0.15066053323226541 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2665 0.13203233181979196 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2552 0.12643396277827734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.954308886296134E-5 2 0.0 0.0 0.0 0.0 9.908617772592268E-5 3 0.0 0.0 0.0 0.0 9.908617772592268E-5 4 0.0 0.0 0.0 4.954308886296134E-5 9.908617772592268E-5 5 0.0 0.0 0.0 4.954308886296134E-5 9.908617772592268E-5 6 9.908617772592268E-5 0.0 0.0 4.954308886296134E-5 9.908617772592268E-5 7 9.908617772592268E-5 0.0 0.0 4.954308886296134E-5 9.908617772592268E-5 8 9.908617772592268E-5 0.0 0.0 4.954308886296134E-5 9.908617772592268E-5 9 9.908617772592268E-5 0.0 0.0 4.954308886296134E-5 9.908617772592268E-5 10-11 9.908617772592268E-5 0.0 0.0 7.4314633294442E-5 9.908617772592268E-5 12-13 9.908617772592268E-5 0.0 0.0 9.908617772592268E-5 1.7340081102036468E-4 14-15 9.908617772592268E-5 0.0 0.0 9.908617772592268E-5 2.2294389988332603E-4 16-17 1.9817235545184535E-4 0.0 0.0 1.4862926658888403E-4 2.477154443148067E-4 18-19 1.9817235545184535E-4 0.0 0.0 1.9817235545184535E-4 2.477154443148067E-4 20-21 1.9817235545184535E-4 0.0 0.0 1.9817235545184535E-4 2.9725853317776806E-4 22-23 1.9817235545184535E-4 0.0 0.0 2.9725853317776806E-4 3.2203007760924873E-4 24-25 1.9817235545184535E-4 0.0 0.0 4.211162553351714E-4 3.4680162204072935E-4 26-27 1.9817235545184535E-4 0.0 0.0 4.7065934419813273E-4 3.4680162204072935E-4 28-29 1.9817235545184535E-4 0.0 0.0 0.0020560381878128955 3.963447109036907E-4 30-31 1.9817235545184535E-4 0.0 0.0 0.008348010473408985 3.963447109036907E-4 32-33 1.9817235545184535E-4 0.0 0.0 0.02048606724483451 4.954308886296134E-4 34-35 1.9817235545184535E-4 0.0 0.0 0.04141802228943568 4.954308886296134E-4 36-37 1.9817235545184535E-4 0.0 0.0 0.0771385893596308 4.954308886296134E-4 38-39 1.9817235545184535E-4 0.0 0.0 0.13458380089623448 4.954308886296134E-4 40-41 1.9817235545184535E-4 0.0 0.0 0.19807326927411945 4.954308886296134E-4 42-43 1.9817235545184535E-4 0.0 0.0 0.2578470059872823 5.202024330610941E-4 44-45 2.477154443148067E-4 0.0 0.0 0.33079920433799287 5.94517066355536E-4 46-47 2.477154443148067E-4 0.0 0.0 0.41628580417103267 6.440601552184974E-4 48-49 2.477154443148067E-4 0.0 0.0 0.5090800096113592 7.431463329444201E-4 50-51 2.477154443148067E-4 0.0 0.0 0.6121791775351818 7.926894218073814E-4 52-53 2.477154443148067E-4 0.0 0.0 0.7038338919316602 8.422325106703428E-4 54-55 2.9725853317776806E-4 0.0 0.0 0.8062394566114013 8.917755995333041E-4 56-57 2.9725853317776806E-4 0.0 0.0 0.9231859178724215 8.917755995333041E-4 58-59 2.9725853317776806E-4 0.0 0.0 1.0513538887609024 8.917755995333041E-4 60-61 2.9725853317776806E-4 0.0 0.0 1.1905451969213923 9.165471439647847E-4 62-63 3.963447109036907E-4 0.0 0.0 1.3267391482056732 9.908617772592268E-4 64-65 3.963447109036907E-4 0.0 0.0 1.4619670092571262 9.908617772592268E-4 66-67 3.963447109036907E-4 0.0 0.0 1.6193158594858914 9.908617772592268E-4 68-69 3.963447109036907E-4 0.0 0.0 1.796531488348704 9.908617772592268E-4 70-71 5.202024330610941E-4 0.0 0.0 1.9765463017322742 9.908617772592268E-4 72-73 5.449739774925748E-4 0.0 0.0 2.1600539028806827 0.0013376633992999562 74-75 5.449739774925748E-4 0.0 0.0 2.3428431292405785 0.0013872064881629174 76-77 5.449739774925748E-4 0.0 0.0 2.5373740676609966 0.0013872064881629174 78-79 5.449739774925748E-4 0.0 0.0 2.764479587008811 0.0014367495770258789 80-81 5.945170663555361E-4 0.0 0.0 3.0058782874935908 0.0014615211214573595 82-83 6.440601552184974E-4 0.0 0.0 3.250497288754462 0.0017092365657721661 84-85 6.440601552184974E-4 0.0 0.0 3.4941501997825055 0.0017835511990666082 86-87 6.440601552184974E-4 0.0 0.0 3.74818238792734 0.00185786583236105 88-89 6.688316996499781E-4 0.0 0.0 4.019678514896368 0.0019817235545184536 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 41.024906 1 GTATCAA 6845 0.0 35.64839 1 ATCAACG 8370 0.0 29.067644 3 TCAACGC 8405 0.0 28.946604 4 CAACGCA 8450 0.0 28.736214 5 TATCAAC 8745 0.0 27.929853 2 AACGCAG 8745 0.0 27.929853 6 GTACATG 6235 0.0 25.120594 1 TACATGG 6140 0.0 24.455923 2 ACGCAGA 10015 0.0 24.301619 7 CGCAGAG 10030 0.0 24.169888 8 ACATGGG 6055 0.0 23.54358 3 GAGTACT 6820 0.0 21.709076 12-13 GTAAGGT 1015 0.0 21.535608 4 GCAGAGT 11340 0.0 21.335331 9 GTACTTT 7015 0.0 20.924032 14-15 AGAGTAC 9245 0.0 20.874905 10-11 GGACCGT 230 8.9914465E-8 20.660343 6 AGTACTT 7145 0.0 20.35574 12-13 GAGTACA 4495 0.0 20.184427 1 >>END_MODULE