FastQCFastQC Report
Thu 26 May 2016
SRR937909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018445
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70690.3502200951722737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59580.2951777234455237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38820.19232627096601593No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30010.148678809677747No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28400.1407023723708102No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26060.12910928957687726No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24850.12311457582445892No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20290.10052292730294855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46750.040.6460761
GTATCAA70950.032.607451
ATCAACG85850.026.666853
TATCAAC87350.026.3766162
TCAACGC86900.026.3439834
CAACGCA87900.026.0983145
AACGCAG90300.025.4046696
GTAAGGT11300.023.9580024
TAAGGTG11950.023.4497535
ACGCAGA101350.022.681717
GTACATG55400.022.637811
CGCAGAG101900.022.5126788
TACATGG54700.021.9721972
AGGTAAG12650.021.4054622
GAGTACT72150.020.96656612-13
ACATGGG55100.020.6020893
TCGCGTA1404.856458E-420.3552959
AGAGTAC93100.019.97267710-11
GTACTTT74950.019.92981314-15
GCAGAGT113950.019.7985539
Produced by FastQC (version 0.11.5)