##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937909_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018445 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19620103594599 33.0 31.0 34.0 31.0 34.0 2 32.45201875701344 34.0 31.0 34.0 31.0 34.0 3 32.51981252895174 34.0 31.0 34.0 31.0 34.0 4 36.06765158327326 37.0 35.0 37.0 35.0 37.0 5 36.00929874234869 37.0 35.0 37.0 35.0 37.0 6 36.05286346667856 37.0 35.0 37.0 35.0 37.0 7 36.01100599719091 37.0 35.0 37.0 35.0 37.0 8 35.997818617797364 37.0 35.0 37.0 35.0 37.0 9 37.649357302279725 39.0 37.0 39.0 35.0 39.0 10-11 37.614776721684265 39.0 37.0 39.0 35.0 39.0 12-13 37.55534185969893 39.0 37.0 39.0 35.0 39.0 14-15 38.77270720777628 40.0 38.0 41.0 34.5 41.0 16-17 38.693606216666794 40.0 38.0 41.0 34.0 41.0 18-19 38.700077782649515 40.0 38.0 41.0 34.5 41.0 20-21 38.592092179871145 40.0 38.0 41.0 34.0 41.0 22-23 38.42069464364894 40.0 38.0 41.0 34.0 41.0 24-25 38.36675955995828 40.0 38.0 41.0 34.0 41.0 26-27 38.32038797192888 40.0 38.0 41.0 34.0 41.0 28-29 38.25611597046241 40.0 38.0 41.0 34.0 41.0 30-31 38.03800004458878 40.0 37.5 41.0 33.0 41.0 32-33 37.84704735576149 40.0 37.0 41.0 33.0 41.0 34-35 37.620379054172886 40.0 37.0 41.0 33.0 41.0 36-37 37.47286648880698 40.0 37.0 41.0 32.5 41.0 38-39 37.463677236684674 40.0 37.0 41.0 32.0 41.0 40-41 37.392935403243584 40.0 37.0 41.0 32.0 41.0 42-43 37.21546487518857 40.0 36.5 41.0 31.5 41.0 44-45 36.993864088444326 39.5 36.0 41.0 31.0 41.0 46-47 36.65556703303781 39.0 36.0 41.0 30.5 41.0 48-49 36.28573852643991 39.0 35.0 40.5 30.0 41.0 50-51 35.945656681257105 39.0 35.0 40.0 29.0 41.0 52-53 35.44161322205956 38.0 34.5 40.0 28.0 41.0 54-55 35.161764625739124 38.0 34.0 40.0 27.0 41.0 56-57 34.87118152835475 38.0 34.0 40.0 26.0 41.0 58-59 34.4712925048738 37.5 33.5 40.0 26.0 41.0 60-61 34.037228410979736 37.0 33.0 40.0 25.0 41.0 62-63 33.61196440824496 36.0 33.0 39.0 24.0 41.0 64-65 33.160782186286966 36.0 32.0 39.0 23.0 40.0 66-67 32.6776258456386 35.0 31.5 38.5 22.0 40.0 68-69 32.1630311948059 35.0 31.0 38.0 20.5 40.0 70-71 31.56667583213811 35.0 30.5 37.5 19.0 39.5 72-73 31.09271518421359 34.0 30.0 37.0 18.0 39.0 74-75 30.59697291727047 34.0 29.5 36.0 15.5 39.0 76-77 29.43394865849701 32.5 28.5 35.0 14.0 37.0 78-79 30.222397191897723 34.0 30.0 35.0 15.0 37.0 80-81 30.044704710804606 34.0 30.0 35.0 11.0 37.0 82-83 29.60846740931757 34.0 29.0 35.0 7.0 36.5 84-85 29.138206886984783 34.0 29.0 35.0 4.5 36.0 86-87 28.59278305824533 33.5 28.5 35.0 2.0 36.0 88-89 27.966965659207958 33.0 27.0 35.0 2.0 35.0 90-91 27.473996566663942 33.0 26.0 35.0 2.0 35.0 92-93 26.88509743887002 32.0 25.0 34.0 2.0 35.0 94-95 26.25419617576897 32.0 24.5 34.0 2.0 35.0 96-97 25.40396592426348 32.0 21.5 34.0 2.0 35.0 98-99 24.378988528297775 31.0 10.0 34.0 2.0 35.0 100-101 21.740341946399333 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1359444573513997 1101 2 0.1940064337902072 1101 3 0.22902320018716438 1101 4 0.054056769134611216 1101 5 0.0494397942226783 1101 6 0.11294842107557201 1101 7 0.07649384316764696 1101 8 0.10979940898190677 1101 9 0.1320180863551741 1101 10-11 0.12962144881544901 1101 12-13 0.16060024879838863 1101 14-15 0.21230381211724847 1101 16-17 0.1261873360389103 1101 18-19 0.2164068551197289 1101 20-21 0.20102001070247155 1101 22-23 0.2766128076296397 1101 24-25 0.23643281544975991 1101 26-27 0.22331008698250088 1101 28-29 0.31263140107702014 1101 30-31 0.1810616677069632 1101 32-33 0.26758667497155386 1101 34-35 0.2912127705670571 1101 36-37 0.4615012776367564 1101 38-39 0.3851059560618424 1101 40-41 0.5307808481179066 1101 42-43 0.5824695562532654 1101 44-45 0.5286298990170906 1101 46-47 0.4978443403630308 1101 48-49 0.5669619272522439 1101 50-51 0.6130872166401389 1101 52-53 0.4439712769938424 1101 54-55 0.5703189485262925 1101 56-57 0.5773601466850735 1101 58-59 0.5545331433746554 1101 60-61 0.47730409663860485 1101 62-63 0.591563937868024 1101 64-65 0.605040473484749 1101 66-67 0.403582922712566 1101 68-69 0.5055786786513465 1101 70-71 0.5508616193020934 1101 72-73 0.3953901956115402 1101 74-75 0.5940485408776368 1101 76-77 0.6037881973868373 1101 78-79 0.5320984842320016 1101 80-81 0.6704584074252757 1101 82-83 0.6485704007861202 1101 84-85 0.8894065863659932 1101 86-87 0.9376780929783486 1101 88-89 0.9232500649046145 1101 90-91 0.946057742049284 1101 92-93 1.2279687258443701 1101 94-95 1.0690489085527126 1101 96-97 1.3618579356306917 1101 98-99 1.3681576949129841 1101 100-101 1.7010072088401245 1102 1 0.24453030527666897 1102 2 0.2064646365004137 1102 3 0.2178790386926508 1102 4 0.15757642388710025 1102 5 0.1920584371718732 1102 6 0.1178736130384408 1102 7 0.19772983556990908 1102 8 0.11994277146956023 1102 9 0.12691586892117357 1102 10-11 0.16605239016598006 1102 12-13 0.28575373888791944 1102 14-15 0.331246072262509 1102 16-17 0.3236225212162793 1102 18-19 0.3216196501492874 1102 20-21 0.3194095235897336 1102 22-23 0.310458023199665 1102 24-25 0.3110106350306836 1102 26-27 0.19881935886744628 1102 28-29 0.25023935073087245 1102 30-31 0.331725541367625 1102 32-33 0.32307276186713807 1102 34-35 0.349780198274388 1102 36-37 0.2550012062918299 1102 38-39 0.2969074477476994 1102 40-41 0.35159659499793605 1102 42-43 0.3617463748381837 1102 44-45 0.39052836069670605 1102 46-47 0.3207922732036721 1102 48-49 0.36202271960024035 1102 50-51 0.44021635601772857 1102 52-53 0.4500982157956628 1102 54-55 0.4657444583751271 1102 56-57 0.5478803398314014 1102 58-59 0.5311094932783007 1102 60-61 0.4407617052299386 1102 62-63 0.5394037627450885 1102 64-65 0.7594456636801823 1102 66-67 0.5800920929978375 1102 68-69 0.8290616280589305 1102 70-71 0.805102561381549 1102 72-73 0.6447884075135626 1102 74-75 0.6354582884235533 1102 76-77 0.5199828195838059 1102 78-79 0.6607021416514129 1102 80-81 0.7267907830198546 1102 82-83 0.7143176326171599 1102 84-85 0.6265789238986876 1102 86-87 0.5250768908374965 1102 88-89 0.37683744808257913 1102 90-91 0.47220530474064404 1102 92-93 0.6648554866750764 1102 94-95 0.6699389329563346 1102 96-97 0.8033330052940144 1102 98-99 0.8762523182638517 1102 100-101 1.1197809429883918 1103 1 0.14197223647454393 1103 2 0.23135138032534996 1103 3 0.21393868427686158 1103 4 0.12571582102808065 1103 5 0.15520495784613075 1103 6 0.11838746791045907 1103 7 0.09636355789618278 1103 8 0.055971841888485585 1103 9 0.17924582457918348 1103 10-11 0.08341780341094562 1103 12-13 0.23177456521777628 1103 14-15 0.30574582413944285 1103 16-17 0.2659195005180095 1103 18-19 0.2984865761445761 1103 20-21 0.3169439006407515 1103 22-23 0.35762942021258226 1103 24-25 0.31711806431561484 1103 26-27 0.24172303909283954 1103 28-29 0.2481699242953539 1103 30-31 0.17882970503280404 1103 32-33 0.2816530176740173 1103 34-35 0.25222661169544125 1103 36-37 0.33299188967078663 1103 38-39 0.2173547896292689 1103 40-41 0.33502117358891326 1103 42-43 0.3877288621518886 1103 44-45 0.37219366668848153 1103 46-47 0.4548539678041976 1103 48-49 0.558728285560953 1103 50-51 0.464091398038569 1103 52-53 0.3862889664079674 1103 54-55 0.524364734031856 1103 56-57 0.5323966599261709 1103 58-59 0.4284245615521698 1103 60-61 0.4911298878485155 1103 62-63 0.6585576630796552 1103 64-65 0.6519555485830892 1103 66-67 0.5443367583518537 1103 68-69 0.5697005849815682 1103 70-71 0.6455158063510602 1103 72-73 0.6417100806634437 1103 74-75 0.6473165701312844 1103 76-77 0.6544735269746731 1103 78-79 0.4759909248878955 1103 80-81 0.7784644131403944 1103 82-83 0.8076559284118687 1103 84-85 0.8578764474703782 1103 86-87 0.8813311929713556 1103 88-89 0.486665923006143 1103 90-91 0.47127004084975255 1103 92-93 0.8867043236982433 1103 94-95 0.6758650723214608 1103 96-97 0.8044831757785751 1103 98-99 0.9154361534461053 1103 100-101 0.8929448322774789 1104 1 0.14014869128816798 1104 2 0.10638937085006717 1104 3 0.09200697783187906 1104 4 0.07962661033710106 1104 5 0.04991310122683501 1104 6 0.05382145378692371 1104 7 0.08105561117230309 1104 8 0.06621587514136706 1104 9 -0.029836145564736682 1104 10-11 0.12047420816647048 1104 12-13 0.15412416743463808 1104 14-15 0.19094069318386886 1104 16-17 0.09468950141190646 1104 18-19 0.2016375549592766 1104 20-21 0.1472000014720649 1104 22-23 0.18262718547583034 1104 24-25 0.1592942509608264 1104 26-27 0.2634044550220409 1104 28-29 0.30949780487102174 1104 30-31 0.20356824083328462 1104 32-33 0.18485316069716617 1104 34-35 0.28035971261616055 1104 36-37 0.3298632582235683 1104 38-39 0.36245401194088345 1104 40-41 0.33353283891474206 1104 42-43 0.3721035828097996 1104 44-45 0.3645776838512589 1104 46-47 0.3345714970792457 1104 48-49 0.42665534375350234 1104 50-51 0.5075257414729109 1104 52-53 0.584179374917845 1104 54-55 0.516064921749738 1104 56-57 0.647083349834098 1104 58-59 0.5221605944474916 1104 60-61 0.5172673688532541 1104 62-63 0.5191525500019694 1104 64-65 0.6456893467416691 1104 66-67 0.588231457556283 1104 68-69 0.6523478541332608 1104 70-71 0.6335085658039965 1104 72-73 0.5466130930885811 1104 74-75 0.3723031617107928 1104 76-77 0.508992164679146 1104 78-79 0.6551207183982193 1104 80-81 0.6512855448110813 1104 82-83 0.7537093833148845 1104 84-85 0.7408187018728078 1104 86-87 0.8521634089363133 1104 88-89 0.6767414571082213 1104 90-91 0.8355790602472695 1104 92-93 1.1587142459294029 1104 94-95 1.0820195373254826 1104 96-97 1.1661485016031925 1104 98-99 1.2113309420400071 1104 100-101 1.001814502430335 1105 1 0.07653469414684366 1105 2 0.049386942797433164 1105 3 0.05981038271632144 1105 4 -0.05377811980680036 1105 5 -0.06251864047661115 1105 6 -0.016774422030316316 1105 7 -0.06625045671498242 1105 8 -0.011173217838596372 1105 9 -0.04763064338145284 1105 10-11 -0.006618076068583889 1105 12-13 0.057352383341957136 1105 14-15 0.13561693179228484 1105 16-17 0.05237391628769217 1105 18-19 0.05591968036797823 1105 20-21 0.096382725407679 1105 22-23 0.01914111346464864 1105 24-25 0.04015766142796906 1105 26-27 0.06583938082686558 1105 28-29 -0.12039410400476669 1105 30-31 0.058817168829115474 1105 32-33 0.1007995471629144 1105 34-35 0.18390177992553447 1105 36-37 0.247983460154515 1105 38-39 0.03055562825807101 1105 40-41 0.07187545102807746 1105 42-43 0.09188021879968034 1105 44-45 0.24878194605850013 1105 46-47 0.3273420440012629 1105 48-49 0.3524861206882619 1105 50-51 0.16440480313086425 1105 52-53 0.2604600639742145 1105 54-55 0.42128039564081377 1105 56-57 0.2776991858991593 1105 58-59 0.2248535446787301 1105 60-61 0.12496538980099814 1105 62-63 0.21721592904890485 1105 64-65 0.3304422702937799 1105 66-67 0.40747023289760165 1105 68-69 0.15850986177229487 1105 70-71 0.26468863304885204 1105 72-73 0.2408800975224743 1105 74-75 0.2524185888923576 1105 76-77 0.4382672415815101 1105 78-79 0.5307896251573538 1105 80-81 0.6107818816918105 1105 82-83 0.6813464715032715 1105 84-85 0.7831178054150136 1105 86-87 0.6855020023490113 1105 88-89 0.4853888873139809 1105 90-91 0.35266134331808985 1105 92-93 0.4966925800131712 1105 94-95 0.37104511814344576 1105 96-97 0.6968252844544942 1105 98-99 0.677953985115149 1105 100-101 0.7268613872710681 1106 1 -0.028694410942939896 1106 2 -0.002530314180447135 1106 3 0.02662654429678213 1106 4 0.040589666678485514 1106 5 -0.013062759826070192 1106 6 -0.012415078756781384 1106 7 0.015123573488018849 1106 8 -0.08884555552538842 1106 9 -0.12602700856406557 1106 10-11 -0.032838133759710786 1106 12-13 -0.18896342449352943 1106 14-15 -0.32643460328872465 1106 16-17 -0.22012796797348244 1106 18-19 -0.14819366119787958 1106 20-21 -0.07283899260807658 1106 22-23 -0.09051688322195872 1106 24-25 -0.08815537036468157 1106 26-27 -0.08109470480636816 1106 28-29 -0.045813381848496704 1106 30-31 0.06999360703520097 1106 32-33 0.12620405448327432 1106 34-35 0.1575695840584217 1106 36-37 0.120480881542818 1106 38-39 0.07102572961512266 1106 40-41 0.07513334821752693 1106 42-43 -0.05998024415654868 1106 44-45 -0.008251326357751054 1106 46-47 -0.0976303802018279 1106 48-49 -0.1453082686423457 1106 50-51 -0.1209613687912352 1106 52-53 -0.18410097396768066 1106 54-55 -0.5232676161493259 1106 56-57 -0.42567019249514004 1106 58-59 -0.2014129993835354 1106 60-61 -0.030282665507812112 1106 62-63 -0.10159664320375583 1106 64-65 -0.22412926332830807 1106 66-67 -0.18859571069304337 1106 68-69 -0.3415980853809444 1106 70-71 -0.4216926045805529 1106 72-73 -0.08887013791105502 1106 74-75 -0.18902432813196057 1106 76-77 -0.16868901796014413 1106 78-79 -0.01875392715805191 1106 80-81 -0.3177069238599124 1106 82-83 -0.39409839423335313 1106 84-85 -0.2158302478775731 1106 86-87 0.0054780931306446234 1106 88-89 0.16230090551344034 1106 90-91 -0.1545434778532382 1106 92-93 -0.0037513285844710254 1106 94-95 -0.10771296628278293 1106 96-97 0.15700241293335537 1106 98-99 0.3827102522232977 1106 100-101 0.4834329675607556 1107 1 0.06307389992148416 1107 2 0.04971486627841415 1107 3 0.0970407613528792 1107 4 0.08959237154957833 1107 5 0.03715991927991524 1107 6 0.030350748929116378 1107 7 0.02507247113278055 1107 8 0.017860622669772397 1107 9 -0.018374868155518698 1107 10-11 0.032080324386178916 1107 12-13 0.06663303826842792 1107 14-15 0.11792543786628329 1107 16-17 0.11020859904163416 1107 18-19 0.14490985970141423 1107 20-21 0.12512094798717754 1107 22-23 0.06682820188693483 1107 24-25 0.023305226773132404 1107 26-27 -0.02756758703817752 1107 28-29 0.047932205083697 1107 30-31 -0.04485079470632769 1107 32-33 0.06358377240607638 1107 34-35 0.038192012563122546 1107 36-37 -0.025645672549842402 1107 38-39 -0.035236620046831035 1107 40-41 0.09810125311850726 1107 42-43 0.13128635424358492 1107 44-45 0.13368080423424544 1107 46-47 0.1343205743066349 1107 48-49 0.027572145284295857 1107 50-51 0.07811412883654612 1107 52-53 0.0568940399446376 1107 54-55 0.12740984310302395 1107 56-57 0.21733703623461764 1107 58-59 0.15025371864191328 1107 60-61 0.12599153362221216 1107 62-63 0.12526957042673814 1107 64-65 0.25274332396369914 1107 66-67 0.25222955321533647 1107 68-69 0.30804717324671316 1107 70-71 0.23640268819885435 1107 72-73 0.28877495932441377 1107 74-75 0.3142007445887458 1107 76-77 0.320428093580702 1107 78-79 0.3585732629262566 1107 80-81 0.3516274064988245 1107 82-83 0.36835247037877394 1107 84-85 0.4715112834993498 1107 86-87 0.4730809379784269 1107 88-89 0.6912331165798484 1107 90-91 0.6717230351952566 1107 92-93 0.6960714947103632 1107 94-95 0.3195104605922552 1107 96-97 0.7032303027015949 1107 98-99 0.8481451653896457 1107 100-101 0.7971251886565298 1108 1 0.00979330429561287 1108 2 0.05988483517459997 1108 3 0.024251397879048397 1108 4 0.05266577166224096 1108 5 -0.006503290046332211 1108 6 0.06690079668694437 1108 7 0.09033997621470036 1108 8 0.06787470510713689 1108 9 0.02601236213519087 1108 10-11 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86-87 -0.29810616461875483 2316 88-89 -0.43822463443326143 2316 90-91 -0.464521713281254 2316 92-93 -0.545011143585608 2316 94-95 -0.3513605587674036 2316 96-97 -0.18298789370848212 2316 98-99 0.01274225172911514 2316 100-101 0.11445926613649249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 20.0 5 30.0 6 92.0 7 223.0 8 530.0 9 1034.0 10 1725.0 11 2802.0 12 4042.0 13 5998.0 14 8077.0 15 10670.0 16 13255.0 17 17901.0 18 17777.0 19 14318.0 20 13600.0 21 13451.0 22 14451.0 23 16011.0 24 18519.0 25 21446.0 26 25840.0 27 31484.0 28 38997.0 29 46853.0 30 57126.0 31 69998.0 32 87661.0 33 114413.0 34 155902.0 35 226346.0 36 330282.0 37 406349.0 38 219320.0 39 11886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36481796630574 18.79412121707552 14.198008863258599 23.643051953360136 2 16.326373872490045 19.861963646165528 39.23491847045136 24.57674401089307 3 20.249315645399033 22.951259088600693 29.357016337804865 27.442408928195412 4 13.553354190973746 16.153375494501958 35.716999967796994 34.576270346727306 5 14.003757349720875 36.93875083975578 34.171966128364225 14.885525682159129 6 33.976568089082484 35.46474412963649 16.201192601825962 14.357495179455066 7 29.746760501276974 32.01746889313308 19.632390280636827 18.603380324953118 8 26.961316660391194 35.30340262777194 18.691712411565366 19.043568300271495 9 26.029596167727803 16.57024717640432 19.739085242690262 37.66107141317762 10-11 25.73637131554241 26.077277310008444 26.51865668868857 21.667694685760573 12-13 26.336387664761734 23.14908258585198 27.330147712719445 23.18438203666684 14-15 22.62154777563917 24.867608480785954 25.344312081825365 27.166531661749517 16-17 22.62260028105871 28.05692219162378 26.37218009259133 22.948297434726182 18-19 22.704978337284395 26.49358788572391 28.554134494623334 22.247299282368356 20-21 22.842534723512404 25.711698857288656 29.013869587727186 22.43189683147175 22-23 22.572128544498362 25.693962431475715 28.98283084255454 22.751078181471378 24-25 22.620575564055894 25.897806154084734 28.949580362776434 22.532037919082935 26-27 22.190814069101737 26.245463760004377 28.80671894972061 22.757003221173274 28-29 22.308608854836272 26.675510108028703 28.65592077069229 22.359960266442734 30-31 22.420254205588954 26.167767761816645 28.58452917964076 22.827448852953637 32-33 22.093109668520743 26.219272635212292 28.70409133403718 22.983526362229785 34-35 22.814518532686616 26.341158611592657 28.49821601692486 22.346106838795865 36-37 22.88868938886106 25.852627281595776 28.546001519040775 22.71268181050239 38-39 22.461248549391065 26.19740272868088 28.5345306865109 22.806818035417155 40-41 22.8674832162056 26.099376315240512 28.5582907658286 22.474849702725294 42-43 22.569931053071375 26.097617712803128 28.441431775340675 22.891019458784818 44-45 22.58610383565329 26.32809343479697 28.54401636154334 22.541786368006406 46-47 23.054826859290195 26.57704321891357 27.85693937659931 22.51119054519692 48-49 22.751994728615347 26.688316996499783 27.827535553359144 22.73215272152573 50-51 22.848603751898118 26.858373649021893 27.840342441830217 22.452680157249763 52-53 23.17888275380305 26.597826544691582 27.747969352645228 22.475321348860135 54-55 22.628112234913512 26.838283926487964 27.903460337041636 22.63014350155689 56-57 22.799902895545827 26.98044781998023 27.602931959998912 22.61671732447503 58-59 22.756131576535402 26.57686981810255 27.66112031786846 23.00587828749359 60-61 22.44170636306662 26.806477263437944 27.982506335322487 22.76931003817295 62-63 22.56769443804513 26.766050102925764 28.091476359276573 22.574779099752533 64-65 22.239174215794833 26.860627859565163 27.97784928496937 22.922348639670638 66-67 22.23194092482084 26.90799105251815 27.82451342493851 23.035554597722506 68-69 22.691974323741523 26.512250423036992 28.005135648041147 22.790639605180335 70-71 22.54250177735831 26.73984180891726 27.602436529110282 23.115219884614145 72-73 22.54175863102537 26.834890224900853 27.62430980284328 22.9990413412305 74-75 22.463827352243932 27.196852032133645 27.444072045559825 22.8952485700626 76-77 22.575224987552296 26.800135748063486 27.648660231019424 22.975979033364794 78-79 22.650604797257294 26.960407640535166 27.617051740324854 22.771935821882686 80-81 22.806715070264485 26.947997096774994 27.40317422570345 22.842113607257073 82-83 22.657791837022852 27.07825464436069 27.501452233591 22.762501285025458 84-85 22.51224000214033 27.266667063023746 27.442451428916826 22.778641505919097 86-87 22.754479892966607 27.207978654755134 27.157667411220228 22.879874041058038 88-89 22.567741571102083 27.391311025379462 26.9612518163753 23.07969558714316 90-91 22.589359984978476 27.333897466207763 27.058089997359343 23.01865255145442 92-93 22.68814596622787 26.993972323668853 27.36534832973691 22.952533380366365 94-95 22.477154443148066 26.76602533138134 27.503796239184126 23.253023986286472 96-97 22.73249952314777 26.506023201028516 27.45933131692962 23.302145958894098 98-99 22.4040783870752 26.877422966689707 27.088426982157053 23.630071664078038 100-101 22.62546167965934 26.56470698978669 27.370971217942525 23.43886011261144 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 437.0 1 316.0 2 326.0 3 491.5 4 386.5 5 314.5 6 688.0 7 1478.0 8 2342.0 9 2681.5 10 2212.5 11 1673.5 12 1591.5 13 1694.0 14 1838.0 15 1960.0 16 2062.0 17 2292.0 18 2593.5 19 2865.5 20 3243.0 21 3906.0 22 4826.5 23 5898.5 24 7320.0 25 9300.5 26 11678.5 27 14483.0 28 17172.5 29 19590.0 30 22168.0 31 26146.5 32 32444.0 33 34933.0 34 34767.0 35 37157.0 36 40820.0 37 44648.5 38 48000.5 39 50889.5 40 53818.0 41 57459.5 42 59693.5 43 62117.5 44 65706.0 45 70002.0 46 72201.0 47 74139.0 48 76765.0 49 75931.0 50 74400.5 51 73223.5 52 72724.5 53 73801.5 54 74493.5 55 69731.5 56 63830.0 57 59844.0 58 55397.5 59 49101.5 60 39837.0 61 32997.0 62 28048.0 63 21058.0 64 14878.5 65 11351.0 66 8769.0 67 6911.5 68 5325.5 69 4028.0 70 3199.0 71 2250.5 72 1572.5 73 1255.0 74 991.0 75 724.0 76 497.5 77 341.5 78 222.5 79 148.5 80 103.0 81 60.0 82 36.5 83 19.5 84 9.5 85 5.0 86 4.5 87 3.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013921607970492135 3 0.005945170663555361 4 0.0 5 4.9543088862961345E-5 6 4.9543088862961345E-5 7 0.0 8 2.477154443148067E-4 9 3.9634471090369076E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.724869887462874E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004681821897549846 26-27 0.019396119289849367 28-29 0.0 30-31 0.0 32-33 3.9634471090369076E-4 34-35 0.017265766468742026 36-37 0.003269843864955448 38-39 0.016200590058188358 40-41 0.013772978703903253 42-43 0.007604864140464565 44-45 0.0026505552541684317 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2294389988332603E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2385772215740335E-4 84-85 0.0031212145983665644 86-87 4.706593441981327E-4 88-89 9.908617772592269E-5 90-91 3.9634471090369076E-4 92-93 2.724869887462874E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.60639542481613 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7036351180798 46.12536361100631 2 12.381037944481305 14.512160110878478 3 3.825010277781534 6.72510194530951 4 1.6764037404654664 3.9299192202143973 5 0.9152910185360975 2.6820953680554616 6 0.5559259192667412 1.9548488550870626 7 0.3710816298113773 1.522342971212757 8 0.2459837545068433 1.1532976947767164 9 0.19913686064446348 1.0503624238727338 >10 1.0310364868702964 10.848774813412843 >50 0.06218471525452009 2.4549972748423086 >100 0.02795425265089556 3.0709531924767988 >500 0.0031234352552342334 1.2894372107291532 >1k 0.0020260120574492235 2.0358493351304334 >5k 1.688343381207686E-4 0.6444959729949977 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7069 0.3502200951722737 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5958 0.2951777234455237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3882 0.19232627096601593 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3001 0.148678809677747 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2840 0.1407023723708102 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2606 0.12910928957687726 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2485 0.12311457582445892 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2029 0.10052292730294855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.954308886296134E-5 2 0.0 0.0 0.0 0.0 4.954308886296134E-5 3 0.0 0.0 0.0 0.0 4.954308886296134E-5 4 0.0 0.0 0.0 0.0 4.954308886296134E-5 5 4.954308886296134E-5 0.0 0.0 0.0 9.908617772592268E-5 6 9.908617772592268E-5 0.0 0.0 0.0 9.908617772592268E-5 7 9.908617772592268E-5 0.0 0.0 0.0 9.908617772592268E-5 8 9.908617772592268E-5 0.0 0.0 0.0 9.908617772592268E-5 9 9.908617772592268E-5 0.0 0.0 0.0 9.908617772592268E-5 10-11 9.908617772592268E-5 0.0 0.0 0.0 1.4862926658888403E-4 12-13 9.908617772592268E-5 0.0 0.0 0.0 1.7340081102036468E-4 14-15 9.908617772592268E-5 0.0 0.0 0.0 2.477154443148067E-4 16-17 1.4862926658888403E-4 0.0 0.0 4.954308886296134E-5 3.7157316647221003E-4 18-19 1.4862926658888403E-4 0.0 0.0 1.4862926658888403E-4 5.449739774925748E-4 20-21 1.4862926658888403E-4 0.0 0.0 1.4862926658888403E-4 6.192886107870167E-4 22-23 1.4862926658888403E-4 0.0 0.0 2.724869887462874E-4 6.936032440814587E-4 24-25 1.9817235545184535E-4 0.0 0.0 3.963447109036907E-4 7.431463329444201E-4 26-27 1.9817235545184535E-4 0.0 0.0 4.4588779976665206E-4 7.431463329444201E-4 28-29 2.2294389988332603E-4 0.0 0.0 0.0020064950989499343 7.431463329444201E-4 30-31 2.477154443148067E-4 0.0 0.0 0.008323238928977504 7.431463329444201E-4 32-33 2.477154443148067E-4 0.0 0.0 0.020535610333697475 7.431463329444201E-4 34-35 2.9725853317776806E-4 0.0 0.0 0.04141802228943568 7.926894218073814E-4 36-37 2.9725853317776806E-4 0.0 0.0 0.07696518854861045 7.926894218073814E-4 38-39 2.9725853317776806E-4 0.0 0.0 0.13433608545191966 8.17460966238862E-4 40-41 2.9725853317776806E-4 0.0 0.0 0.19772646765207869 8.422325106703428E-4 42-43 3.4680162204072935E-4 0.0 0.0 0.25764883363183044 8.670040551018235E-4 44-45 3.963447109036907E-4 0.0 0.0 0.33050194580481507 9.165471439647847E-4 46-47 4.954308886296134E-4 0.0 0.0 0.4154435716603623 9.413186883962655E-4 48-49 4.954308886296134E-4 0.0 0.0 0.5078166608453537 9.413186883962655E-4 50-51 4.954308886296134E-4 0.0 0.0 0.610593798691567 9.908617772592268E-4 52-53 4.954308886296134E-4 0.0 0.0 0.7016044529328269 0.001040404866122188 54-55 4.954308886296134E-4 0.0 0.0 0.8030439273797403 0.001040404866122188 56-57 4.954308886296134E-4 0.0 0.0 0.9188508975969125 0.001040404866122188 58-59 4.954308886296134E-4 0.0 0.0 1.04545826118621 0.001040404866122188 60-61 5.449739774925748E-4 0.0 0.0 1.183262362858537 0.001040404866122188 62-63 5.945170663555361E-4 0.0 0.0 1.3184902239099903 0.0010899479549851495 64-65 5.945170663555361E-4 0.0 0.0 1.4526529085508892 0.0010899479549851495 66-67 5.945170663555361E-4 0.0 0.0 1.6088127246469435 0.0010899479549851495 68-69 5.945170663555361E-4 0.0 0.0 1.7840466299552378 0.0010899479549851495 70-71 5.945170663555361E-4 0.0 0.0 1.9619310905177003 0.0011642625882795914 72-73 8.17460966238862E-4 0.0 0.0 2.1433331103894333 0.001263348766005514 74-75 8.422325106703428E-4 0.0 0.0 2.3239176692949277 0.0012881203104369947 76-77 8.422325106703428E-4 0.0 0.0 2.5175320605713805 0.0012881203104369947 78-79 8.422325106703428E-4 0.0 0.0 2.7422595116537734 0.0012881203104369947 80-81 9.413186883962655E-4 0.0 0.0 2.9819242040283487 0.0012881203104369947 82-83 9.413186883962655E-4 0.0 0.0 3.223768792312894 0.001511064210320321 84-85 9.660902328277462E-4 0.0 0.0 3.4645729757313175 0.0015358357547518016 86-87 9.908617772592268E-4 0.0 0.0 3.7149637468447247 0.0015606072991832822 88-89 9.908617772592268E-4 0.0 0.0 3.9823230258936952 0.0015853788436147628 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4675 0.0 40.646076 1 GTATCAA 7095 0.0 32.60745 1 ATCAACG 8585 0.0 26.66685 3 TATCAAC 8735 0.0 26.376616 2 TCAACGC 8690 0.0 26.343983 4 CAACGCA 8790 0.0 26.098314 5 AACGCAG 9030 0.0 25.404669 6 GTAAGGT 1130 0.0 23.958002 4 TAAGGTG 1195 0.0 23.449753 5 ACGCAGA 10135 0.0 22.68171 7 GTACATG 5540 0.0 22.63781 1 CGCAGAG 10190 0.0 22.512678 8 TACATGG 5470 0.0 21.972197 2 AGGTAAG 1265 0.0 21.405462 2 GAGTACT 7215 0.0 20.966566 12-13 ACATGGG 5510 0.0 20.602089 3 TCGCGTA 140 4.856458E-4 20.355295 9 AGAGTAC 9310 0.0 19.972677 10-11 GTACTTT 7495 0.0 19.929813 14-15 GCAGAGT 11395 0.0 19.798553 9 >>END_MODULE