FastQCFastQC Report
Thu 26 May 2016
SRR937908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060516
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68670.3332660362744089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.26765140382312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36120.17529589675595822No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32220.15636859893347102No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30920.15005949965930862No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26810.13011303964637982No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25830.12535694942431896No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA20690.1004117415249384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.041.5218051
GTATCAA67500.036.753891
ATCAACG80750.030.099643
CAACGCA81300.029.9545175
TCAACGC81400.029.8592834
TATCAAC82650.029.637892
AACGCAG84250.029.0686666
ACGCAGA96400.025.101017
CGCAGAG97200.024.8449028
TACATGG57250.024.5922972
GTAAGGT10350.024.3566994
GTACATG59750.023.9085241
TAAGGTG11350.023.8870165
ACCGTAC1000.001646430123.7689448
ACATGGG56850.023.6776643
GCAGAGT107950.022.1054739
GTGTAAG7700.021.6444571
TATACCG1100.002855558221.6202265
GTGTACG2657.694325E-1021.562781
CATGGGG39950.021.0736314
Produced by FastQC (version 0.11.5)