FastQCFastQC Report
Thu 26 May 2016
SRR937908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060516
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70280.34107961306779466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.27857099872070884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.1858272393905216No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30990.15039922038945583No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28740.13947962549186707No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25730.12487163409553724No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25350.1230274358461667No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20680.10036320999206023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45100.041.612031
GTATCAA70000.035.022731
ATCAACG85250.028.580763
TCAACGC86050.028.3122984
CAACGCA87100.028.0800385
TATCAAC87700.027.898762
AACGCAG90950.026.94366
GTAAGGT10950.025.5885984
TAAGGTG12150.024.233945
ACGCAGA102450.023.7331897
CGCAGAG103900.023.310568
GTACATG60300.022.4556641
TACATGG59050.021.642612
ACATGGG57400.021.2653243
GAGTACT71700.020.89672912-13
GCAGAGT114800.020.8077059
AGGCGAC7700.020.353165
GTACTTT73050.020.21800414-15
AGGTAAG12700.019.8266542
AGAGTAC95550.019.70654910-11
Produced by FastQC (version 0.11.5)