FastQCFastQC Report
Thu 26 May 2016
SRR937907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725414
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73740.27056439865649773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60380.22154432317438746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.14394143421880126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38260.14038234191209115No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28840.10581878569641164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111250.031.8580551
GGTATCA74500.031.8006531
ATCAACG130800.026.828953
TCAACGC131000.026.7874974
GTACATG103400.026.7772161
TACATGG100800.026.6528112
TATCAAC133950.026.2335342
CAACGCA133700.026.2124235
AACGCAG137600.025.4777776
ACATGGG103050.025.194153
CATGGGG53900.022.5838644
ACGCAGA156500.022.2495487
CGCAGAG156700.022.1900228
GAGTACT79900.022.14558412-13
GAGTACA74850.021.0377451
GCAGAGT165500.020.5505149
GTACTTT86000.020.5191814-15
AGAGTAC125650.020.35761510-11
AGTACTT85550.020.2665812-13
CATGGGA50500.019.396484
Produced by FastQC (version 0.11.5)