##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937907_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2725414 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.297220165450092 31.0 31.0 34.0 28.0 34.0 2 31.4394748834489 33.0 31.0 34.0 28.0 34.0 3 31.461578681257233 33.0 31.0 34.0 28.0 34.0 4 35.0610813623178 37.0 35.0 37.0 32.0 37.0 5 35.03671772435307 37.0 35.0 37.0 32.0 37.0 6 35.08744909947626 37.0 35.0 37.0 32.0 37.0 7 35.0009495805041 37.0 35.0 37.0 32.0 37.0 8 34.980772829375645 37.0 35.0 37.0 32.0 37.0 9 36.55399363179319 39.0 37.0 39.0 32.0 39.0 10-11 36.40433031458707 39.0 37.0 39.0 32.0 39.0 12-13 36.26066498520959 39.0 37.0 39.0 32.0 39.0 14-15 37.33885439056232 40.0 37.0 41.0 32.0 41.0 16-17 37.22285054674262 40.0 37.0 41.0 31.5 41.0 18-19 37.11741041911431 40.0 37.0 41.0 31.0 41.0 20-21 36.98128431130097 39.0 36.0 41.0 31.0 41.0 22-23 36.881697055933515 39.0 36.0 41.0 30.5 41.0 24-25 36.90880064459931 39.0 36.0 41.0 30.5 41.0 26-27 36.80267768493154 39.0 36.0 41.0 30.0 41.0 28-29 36.661443729282965 39.0 36.0 41.0 30.0 41.0 30-31 36.41069980560752 39.0 36.0 40.5 30.0 41.0 32-33 36.24983580476214 39.0 35.0 40.5 30.0 41.0 34-35 36.23108287401474 39.0 35.0 41.0 29.5 41.0 36-37 36.065570405083406 39.0 35.0 41.0 29.0 41.0 38-39 35.87874044090182 39.0 35.0 41.0 27.5 41.0 40-41 35.62185396420507 39.0 35.0 40.5 27.0 41.0 42-43 35.322989644875975 38.0 35.0 40.0 26.0 41.0 44-45 35.094383275348264 38.0 34.5 40.0 25.5 41.0 46-47 34.75712222069748 38.0 34.0 40.0 24.0 41.0 48-49 34.42540619516888 38.0 33.5 40.0 23.0 41.0 50-51 33.51795030039473 37.5 32.5 39.5 22.0 40.5 52-53 33.60334411579305 37.5 33.0 39.5 22.0 40.5 54-55 33.84755600433549 38.0 33.0 40.0 21.5 41.0 56-57 33.55934658000582 38.0 33.0 40.0 19.5 41.0 58-59 33.25550044873916 37.0 32.5 40.0 18.5 41.0 60-61 32.873936400121224 37.0 32.0 40.0 15.5 41.0 62-63 32.45339258549343 36.0 31.0 39.0 12.5 41.0 64-65 32.01030467297812 36.0 31.0 39.0 8.0 40.5 66-67 31.524704320150995 35.0 30.5 39.0 7.5 40.0 68-69 30.96731047099633 35.0 30.0 38.0 4.5 40.0 70-71 30.47140434444088 35.0 29.0 37.5 2.0 40.0 72-73 29.97620673409618 34.0 29.0 37.0 2.0 39.0 74-75 29.413261618234884 34.0 28.5 36.0 2.0 39.0 76-77 28.896958223594652 34.0 28.0 36.0 2.0 38.5 78-79 28.307405773948474 34.0 26.5 35.0 2.0 37.5 80-81 27.713875580003624 33.0 26.0 35.0 2.0 37.0 82-83 27.17406419721921 33.0 25.5 35.0 2.0 36.5 84-85 26.62484103332558 32.5 24.5 35.0 2.0 36.0 86-87 26.04532118790026 32.0 23.5 35.0 2.0 35.5 88-89 25.49554618124072 32.0 20.5 34.5 2.0 35.0 90-91 24.8702444839573 31.5 18.5 34.0 2.0 35.0 92-93 24.16520866187669 31.0 11.0 34.0 2.0 35.0 94-95 23.35701897032891 31.0 2.0 34.0 2.0 35.0 96-97 22.436389847560775 30.0 2.0 34.0 2.0 35.0 98-99 21.305845460542876 29.5 2.0 34.0 2.0 35.0 100-101 18.566094362177637 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30633692751953134 1101 2 0.3462103792267186 1101 3 0.3253762936944753 1101 4 0.2786739798910105 1101 5 0.20207082152125366 1101 6 0.31088705612557277 1101 7 0.38701641698934 1101 8 0.27835455495864636 1101 9 0.47801172437013406 1101 10-11 0.4279768406456981 1101 12-13 0.4301091194420721 1101 14-15 0.5618974091584832 1101 16-17 0.6478090636392153 1101 18-19 0.6039881567698728 1101 20-21 0.5653353122405349 1101 22-23 0.5329249448763917 1101 24-25 0.6262699353092103 1101 26-27 0.517173626977872 1101 28-29 0.6287988923977537 1101 30-31 0.7448927673167205 1101 32-33 0.6623230115266381 1101 34-35 0.6276909324658178 1101 36-37 0.6658319775835722 1101 38-39 0.5667775750168929 1101 40-41 0.6090437585169113 1101 42-43 0.6355347704043695 1101 44-45 0.8609065825801778 1101 46-47 0.9349613961774352 1101 48-49 1.0502006412132374 1101 50-51 0.9523609366339514 1101 52-53 0.896963020641742 1101 54-55 1.0076387762706958 1101 56-57 0.898871535984604 1101 58-59 0.8258925984196068 1101 60-61 0.9345226914101232 1101 62-63 0.8996619084621003 1101 64-65 0.9765805878589369 1101 66-67 1.0207268108672238 1101 68-69 1.0485676483488184 1101 70-71 1.0835608803932502 1101 72-73 1.2268540037837 1101 74-75 1.43698309926949 1101 76-77 1.3805351747383696 1101 78-79 1.5135691719485642 1101 80-81 1.4850331330330313 1101 82-83 1.365189324861337 1101 84-85 1.3373432386034878 1101 86-87 1.4926371734388084 1101 88-89 1.4301577762592075 1101 90-91 1.5568218649765448 1101 92-93 1.5706863496355403 1101 94-95 1.6885294052633988 1101 96-97 1.9889193177791071 1101 98-99 2.1319084614941204 1101 100-101 2.0343523790735816 1102 1 0.2047612965781056 1102 2 0.3984772271904724 1102 3 0.32671134725017836 1102 4 0.16143181309331567 1102 5 0.18604873512601472 1102 6 0.2366423549310923 1102 7 0.11681139465751045 1102 8 0.31569182397876716 1102 9 0.3282779607187152 1102 10-11 0.39003760316970215 1102 12-13 0.42373335935271683 1102 14-15 0.5385075254834888 1102 16-17 0.62146170559366 1102 18-19 0.6163847170251202 1102 20-21 0.589691902262814 1102 22-23 0.47822476099809563 1102 24-25 0.5545598277778083 1102 26-27 0.3648620234830062 1102 28-29 0.5614082186104454 1102 30-31 0.6238115340590227 1102 32-33 0.6441821904135807 1102 34-35 0.5701620940227841 1102 36-37 0.7891114161575885 1102 38-39 0.8004903022562075 1102 40-41 0.7618084953583235 1102 42-43 0.7783664699202006 1102 44-45 0.7866576158292133 1102 46-47 0.7775972082147007 1102 48-49 0.7748603482416954 1102 50-51 0.8019248655133424 1102 52-53 0.745813879724821 1102 54-55 0.7854353079139003 1102 56-57 0.8842589206997431 1102 58-59 0.8892473995331116 1102 60-61 0.8066781886624526 1102 62-63 0.9767926195500465 1102 64-65 0.8939011752720027 1102 66-67 1.014277814454232 1102 68-69 0.9863159201422036 1102 70-71 0.9500699620911277 1102 72-73 0.8913733018174739 1102 74-75 1.0529081934726605 1102 76-77 0.9542462220046737 1102 78-79 1.130286609674723 1102 80-81 1.1417038727592725 1102 82-83 1.1984630935264242 1102 84-85 1.3395190005303128 1102 86-87 1.2617999562616085 1102 88-89 1.3368825901168826 1102 90-91 1.3284613611421356 1102 92-93 1.4946720331154992 1102 94-95 1.5074757603125732 1102 96-97 1.8535117208886334 1102 98-99 1.8409314915616974 1102 100-101 1.6895413301010578 1103 1 0.15874676809226074 1103 2 0.3848038721938245 1103 3 0.3180106093409165 1103 4 0.1753695720512809 1103 5 0.16646374198578684 1103 6 0.19301357909147043 1103 7 0.24807497844223292 1103 8 0.2180628459038232 1103 9 0.17372548127618614 1103 10-11 0.27123096635563826 1103 12-13 0.3557751746269773 1103 14-15 0.39597175667092444 1103 16-17 0.35313881068236697 1103 18-19 0.347132538314014 1103 20-21 0.3099409013852039 1103 22-23 0.29439534774270015 1103 24-25 0.28259969310406063 1103 26-27 0.39310012936193317 1103 28-29 0.32024396732432336 1103 30-31 0.22791416474112225 1103 32-33 0.2833142263615329 1103 34-35 0.552926151562481 1103 36-37 0.4656223449794865 1103 38-39 0.44904438210421205 1103 40-41 0.5825327474933459 1103 42-43 0.5589549996818732 1103 44-45 0.46611749770880095 1103 46-47 0.455524283654718 1103 48-49 0.37168512881012816 1103 50-51 0.42693725554789097 1103 52-53 0.4822904535088739 1103 54-55 0.40423901106740345 1103 56-57 0.4079059102022242 1103 58-59 0.36550865511487274 1103 60-61 0.4468255832597734 1103 62-63 0.5720161832111117 1103 64-65 0.6797827777247925 1103 66-67 0.5958808290089337 1103 68-69 0.7097674154976552 1103 70-71 0.6685958413466508 1103 72-73 0.6669432121972676 1103 74-75 0.6112121381850208 1103 76-77 0.6335041806512933 1103 78-79 0.7352589146705562 1103 80-81 0.7978119413245359 1103 82-83 0.9220530326935403 1103 84-85 0.8625785088987357 1103 86-87 0.8937688669497454 1103 88-89 1.0115767091885495 1103 90-91 1.1092457595607073 1103 92-93 0.9825015237696029 1103 94-95 1.2084213352913196 1103 96-97 1.3866584650871658 1103 98-99 1.4978465040391207 1103 100-101 1.202535761139437 1104 1 0.21301342676362722 1104 2 0.314931329523894 1104 3 0.15441854924621268 1104 4 0.2595177848272101 1104 5 0.30257522671520576 1104 6 0.1558864445198367 1104 7 0.23908468675795547 1104 8 0.27977229976357876 1104 9 0.32598097981612995 1104 10-11 0.2854912271951022 1104 12-13 0.3280696717509386 1104 14-15 0.42295069471205693 1104 16-17 0.43128618920778194 1104 18-19 0.3363109926714074 1104 20-21 0.4365822570375286 1104 22-23 0.3526612486126126 1104 24-25 0.33229774315096705 1104 26-27 0.30728108623199546 1104 28-29 0.33371381640667863 1104 30-31 0.31871452204497785 1104 32-33 0.2540317224250046 1104 34-35 0.4216856673818228 1104 36-37 0.4081446460106193 1104 38-39 0.5742907810541809 1104 40-41 0.5744819432610342 1104 42-43 0.39352338373717544 1104 44-45 0.35094601168452755 1104 46-47 0.4790925179176142 1104 48-49 0.4803995348975718 1104 50-51 0.504963928152101 1104 52-53 0.3972306452479373 1104 54-55 0.5433053122437883 1104 56-57 0.6282488058016966 1104 58-59 0.5649930180539897 1104 60-61 0.6382401491496736 1104 62-63 0.6503982842849112 1104 64-65 0.7832908824017331 1104 66-67 0.9444780362764646 1104 68-69 0.7081380348761108 1104 70-71 0.6274267008755388 1104 72-73 0.7091195094962472 1104 74-75 0.7691012167897178 1104 76-77 0.7948729463786002 1104 78-79 0.6454424211172451 1104 80-81 0.9249086467755738 1104 82-83 0.8515551007604536 1104 84-85 0.8776626876911422 1104 86-87 1.0410072755380355 1104 88-89 1.1129232162900742 1104 90-91 1.0390213122457048 1104 92-93 1.1939407301517626 1104 94-95 1.1139879149095684 1104 96-97 1.072114840975793 1104 98-99 1.1680672961327545 1104 100-101 1.3338098709676096 1105 1 0.26010871896680143 1105 2 0.38790204266429384 1105 3 0.3076426828887904 1105 4 0.2664129408252407 1105 5 0.27051952027951387 1105 6 0.33103510913118583 1105 7 0.35315202111129906 1105 8 0.2652959140093145 1105 9 0.3079816397318922 1105 10-11 0.42051014126194985 1105 12-13 0.3224765070647848 1105 14-15 0.286643499335689 1105 16-17 0.36149051757598727 1105 18-19 0.4808642668723735 1105 20-21 0.4381001091815193 1105 22-23 0.4800154682645186 1105 24-25 0.4125218506355566 1105 26-27 0.42139993348123284 1105 28-29 0.4764909856158752 1105 30-31 0.5956504891060987 1105 32-33 0.5221331704766001 1105 34-35 0.5822936411650872 1105 36-37 0.5891429498710821 1105 38-39 0.606256655424211 1105 40-41 0.6894153023240364 1105 42-43 0.708932829802599 1105 44-45 0.8260944812581812 1105 46-47 0.7991627911447736 1105 48-49 0.6917738691306852 1105 50-51 0.7995339985400491 1105 52-53 0.8046049514873985 1105 54-55 0.6667548199686522 1105 56-57 0.8631602885195306 1105 58-59 0.7822699958967831 1105 60-61 0.9581489426419338 1105 62-63 1.0010564332349574 1105 64-65 0.9640623689009935 1105 66-67 0.9602135624147294 1105 68-69 1.0737534522154668 1105 70-71 0.9157497714020835 1105 72-73 0.8529031305522423 1105 74-75 0.7490374601159893 1105 76-77 0.7818119032527839 1105 78-79 0.7857508147133991 1105 80-81 0.6967848772339416 1105 82-83 0.8619257710384538 1105 84-85 0.8728221390340565 1105 86-87 1.0445904620959467 1105 88-89 1.211621867455296 1105 90-91 1.1558133804988024 1105 92-93 1.1883200880968694 1105 94-95 1.4217462044703204 1105 96-97 1.3324190837109882 1105 98-99 1.2028937997815667 1105 100-101 1.2005542697145941 1106 1 0.11046820778492972 1106 2 0.10672964924360784 1106 3 0.09396803669325493 1106 4 0.18418409617491704 1106 5 0.0878110931776277 1106 6 0.0779320107853323 1106 7 0.1825024624874345 1106 8 0.3049070109871934 1106 9 0.35931729992304895 1106 10-11 0.15101228174750503 1106 12-13 0.0640179432122494 1106 14-15 0.19992069501073217 1106 16-17 0.1528098911601461 1106 18-19 0.1453174715901966 1106 20-21 0.2031226462486302 1106 22-23 0.23632754770384423 1106 24-25 0.19561102464295743 1106 26-27 0.07129426040242492 1106 28-29 0.18140805694334006 1106 30-31 0.3380794649624974 1106 32-33 0.3221022559758069 1106 34-35 0.3245734609617372 1106 36-37 0.18314807604044603 1106 38-39 0.3772574325965863 1106 40-41 0.29660877118729445 1106 42-43 0.3708614473583083 1106 44-45 0.4523242470534683 1106 46-47 0.5123781461699721 1106 48-49 0.40283248926764514 1106 50-51 0.4414256747815912 1106 52-53 0.41795540311401425 1106 54-55 0.29688500799032624 1106 56-57 0.38872771055302024 1106 58-59 0.4767143432723415 1106 60-61 0.22511004255517975 1106 62-63 0.24834250349172038 1106 64-65 0.37341136123270857 1106 66-67 0.43176907665776554 1106 68-69 0.3012335662939343 1106 70-71 0.145880856340316 1106 72-73 0.38927181591018467 1106 74-75 0.41025238834868816 1106 76-77 0.42052942671938354 1106 78-79 0.5850431358961927 1106 80-81 0.47023109159374243 1106 82-83 0.35921504456864994 1106 84-85 0.5878521193438289 1106 86-87 0.6456689984399375 1106 88-89 0.8549922912006132 1106 90-91 0.6671098116561431 1106 92-93 0.8167812541722661 1106 94-95 1.0343970029357301 1106 96-97 1.116600632203653 1106 98-99 1.2303258181653298 1106 100-101 1.2276279874034657 1107 1 0.22730974594295716 1107 2 0.3273831053985212 1107 3 0.2904534758436661 1107 4 0.14222174910017316 1107 5 0.17174340624544726 1107 6 0.17151656986987263 1107 7 0.17734768704755055 1107 8 0.194040205494602 1107 9 0.2593633251160412 1107 10-11 0.256737818064515 1107 12-13 0.20507303872750526 1107 14-15 0.1987059392899937 1107 16-17 0.346112642617058 1107 18-19 0.44892045806765424 1107 20-21 0.31817321912780017 1107 22-23 0.25598002806001574 1107 24-25 0.23083109074977415 1107 26-27 0.29619347423395226 1107 28-29 0.27238965875627486 1107 30-31 0.37504460968096964 1107 32-33 0.4165381137453821 1107 34-35 0.49990087531902816 1107 36-37 0.5729446359141264 1107 38-39 0.6145888164103326 1107 40-41 0.5834543508351757 1107 42-43 0.5260288575044285 1107 44-45 0.4971684499858995 1107 46-47 0.55680267368448 1107 48-49 0.5132747132206674 1107 50-51 0.3764575420618854 1107 52-53 0.2965070744449534 1107 54-55 0.4885410341449159 1107 56-57 0.4226103678978177 1107 58-59 0.49852459364961277 1107 60-61 0.5276968269410602 1107 62-63 0.670569046948934 1107 64-65 0.7221446641134612 1107 66-67 0.7562419542897416 1107 68-69 0.7624372260990739 1107 70-71 0.6733089623222419 1107 72-73 0.6477020047427651 1107 74-75 0.5140268466637927 1107 76-77 0.606267284905222 1107 78-79 0.8897752229822764 1107 80-81 0.8857088895462617 1107 82-83 0.8141665160992346 1107 84-85 0.7071715375309005 1107 86-87 0.8623965760498749 1107 88-89 0.9898823542494739 1107 90-91 1.2350679657861683 1107 92-93 1.1187535612511539 1107 94-95 1.21601891365194 1107 96-97 1.451128865894809 1107 98-99 1.370668371659658 1107 100-101 1.3633279645582554 1108 1 -0.0906370062281674 1108 2 -0.047385877642838636 1108 3 0.02261047467704813 1108 4 0.07747379716423097 1108 5 0.015786404516589414 1108 6 0.12716358265556948 1108 7 0.10261325526229825 1108 8 0.04804267356494307 1108 9 0.1749413607161543 1108 10-11 0.14782692162688704 1108 12-13 0.23778572072392734 1108 14-15 0.15654157643654543 1108 16-17 0.2926304205240058 1108 18-19 0.27658882953666364 1108 20-21 0.1805639283620195 1108 22-23 0.13223764300344243 1108 24-25 0.04647231906312754 1108 26-27 0.10742612216744618 1108 28-29 0.2090879329437172 1108 30-31 0.019751223275413565 1108 32-33 0.16727878333964696 1108 34-35 0.17144499882886066 1108 36-37 0.21338593043451937 1108 38-39 0.3284298680995761 1108 40-41 0.32756491744768823 1108 42-43 0.1892799772870788 1108 44-45 0.2327348194051666 1108 46-47 0.2553042857523167 1108 48-49 0.45753947480727675 1108 50-51 0.3302939091280308 1108 52-53 0.42693990293523854 1108 54-55 0.4785616849488292 1108 56-57 0.5288013522888164 1108 58-59 0.4869897896874775 1108 60-61 0.4977918396541483 1108 62-63 0.5429155526155327 1108 64-65 0.7910367680386194 1108 66-67 0.6371182337828287 1108 68-69 0.4665932300982867 1108 70-71 0.5553731978395078 1108 72-73 0.7808899123649375 1108 74-75 0.6029010937046273 1108 76-77 0.520681079305156 1108 78-79 0.588307194346438 1108 80-81 0.5695892592252498 1108 82-83 0.8082463992948128 1108 84-85 0.7942882406107188 1108 86-87 0.8161847031665168 1108 88-89 0.7254933982916292 1108 90-91 0.915990734977516 1108 92-93 0.7934330653984922 1108 94-95 0.9245168912474249 1108 96-97 1.0122446683515136 1108 98-99 1.0909541293466631 1108 100-101 1.0676238806170701 1109 1 0.12449968598217609 1109 2 0.0781409292019859 1109 3 0.22383048761057367 1109 4 0.11505120724874729 1109 5 0.1466728722078443 1109 6 0.14505251419875975 1109 7 0.23955401879580052 1109 8 0.18187422282654353 1109 9 0.3693285697966928 1109 10-11 0.3165551198440042 1109 12-13 0.4002969685770097 1109 14-15 0.4096927272159192 1109 16-17 0.33359039864256346 1109 18-19 0.3382495656001083 1109 20-21 0.31965013295918254 1109 22-23 0.31486866940549163 1109 24-25 0.21254455672290362 1109 26-27 0.3465524128100057 1109 28-29 0.2906779813557492 1109 30-31 0.36411821961630864 1109 32-33 0.35336354939415315 1109 34-35 0.38030528500008387 1109 36-37 0.41422429925110293 1109 38-39 0.2717621495528917 1109 40-41 0.4175792765954327 1109 42-43 0.34873374716011085 1109 44-45 0.4627444637069402 1109 46-47 0.47665885903247585 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2316 72-73 -0.3980354313166572 2316 74-75 -0.5114658342333698 2316 76-77 -0.43594012203545063 2316 78-79 -0.39351492237276275 2316 80-81 -0.3512250411047333 2316 82-83 -0.25983148510071175 2316 84-85 -0.38745225657243054 2316 86-87 -0.47995330888935683 2316 88-89 -0.8314174681179658 2316 90-91 -0.714373023882164 2316 92-93 -0.8131857841466861 2316 94-95 -0.7242054177208566 2316 96-97 -0.9321361175070422 2316 98-99 -0.9445891122865859 2316 100-101 -0.943174394877957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15110.0 3 3793.0 4 3794.0 5 4140.0 6 4800.0 7 5566.0 8 6648.0 9 8449.0 10 10124.0 11 12486.0 12 14894.0 13 17697.0 14 19589.0 15 22536.0 16 25876.0 17 30167.0 18 27984.0 19 24344.0 20 25312.0 21 26900.0 22 29417.0 23 32180.0 24 35947.0 25 40707.0 26 46082.0 27 53728.0 28 62074.0 29 73042.0 30 87102.0 31 104157.0 32 127813.0 33 160183.0 34 206319.0 35 274156.0 36 365410.0 37 430520.0 38 263844.0 39 22522.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0950926739715 18.248150994193864 13.888742631867887 23.768013699966755 2 15.516661391356834 19.26232766298867 40.64735412541497 24.573656820239524 3 19.78276087207749 23.08429802679182 28.988280879084964 28.144660222045726 4 12.810166779167954 15.480937473086184 36.239117616965146 35.469778130780725 5 13.907632869588518 37.13428379180218 34.01685265047167 14.941230688137624 6 33.706010611503515 35.08767543359721 16.735694141611727 14.470619813287545 7 29.83660433525416 31.96117450148107 19.834861467773866 18.3673596954909 8 26.768795079363212 35.105940180493945 18.996318641235924 19.12894609890692 9 26.33460148464522 16.739413473583735 19.924315376619614 37.00166966515143 10-11 25.49781349081225 26.22332821906827 26.669841249412805 21.609017040706668 12-13 26.16420451203012 23.344626889817047 27.543789340549445 22.94737925760339 14-15 22.81351961296898 25.012519385744884 25.57557921921683 26.598381782069296 16-17 22.576870855859042 28.314416485648962 26.31916170802266 22.789550950469327 18-19 22.543428368469932 26.881816303314327 28.43880686794379 22.13594846027195 20-21 22.950955278780373 25.949097753966655 28.843270309841756 22.256676657411212 22-23 22.711531101692202 25.84500862296838 28.793692494396268 22.649767780943147 24-25 22.55050447023961 26.112155356796542 28.884356505170622 22.452983667793227 26-27 22.28430791718833 26.343404806226882 28.78596568367312 22.586321592911666 28-29 22.270504800731715 26.78281572452211 28.654885071999487 22.29179440274669 30-31 22.40657164074251 26.252365551285372 28.601176648605158 22.73988615936696 32-33 22.14545131615454 26.273594888024775 28.806287079388483 22.774666716432208 34-35 22.738066105148043 26.342058132272438 28.582586205660256 22.33728955691926 36-37 22.745624950602057 26.062703680353493 28.534549150723006 22.657122218321444 38-39 22.361007117397172 26.486491354400655 28.576186070515273 22.576315457686903 40-41 22.75421795787501 26.352876651498182 28.443484676324555 22.449420714302256 42-43 22.393531179645723 26.355255328289473 28.307063447550945 22.944150044513858 44-45 22.485135740808353 26.538773922440022 28.366330499647212 22.609759837104413 46-47 22.93475129505197 26.81872140401889 27.77526828998354 22.471259010945595 48-49 22.780513623186486 26.82278726055071 27.71406190223279 22.682637214030006 50-51 22.769183874237246 27.036260592084933 27.723239720546 22.471315813131824 52-53 22.967858418966184 26.855279304474735 27.614311325787575 22.56255095077151 54-55 22.690148433506206 26.925914127349987 27.67581132553406 22.708126113609747 56-57 22.734151326988314 27.107021020312374 27.54836414134007 22.610463511359246 58-59 22.935319679957068 26.77506296928312 27.498789317321997 22.790828033437815 60-61 22.613783611881374 26.987952382903877 27.810337277044106 22.587926728170643 62-63 22.53240634638948 27.02523239151494 27.999605229858958 22.442756032236623 64-65 22.44100616600221 27.043298012658397 27.846196002454565 22.669499818884827 66-67 22.46503859565551 27.005895297545468 27.618599720727783 22.910466386071235 68-69 22.692982154649656 26.75348721631245 27.892970171120023 22.66056045791787 70-71 22.629257149872515 26.875889868815396 27.661023640208775 22.833829341103314 72-73 22.6278110059388 26.953661187925388 27.64523779550423 22.773290010631584 74-75 22.452741276153713 27.36440650864932 27.48056149910513 22.70229071609184 76-77 22.733803534763418 26.99309475509071 27.477301872369225 22.79579983777665 78-79 22.718059822174137 27.07503789639575 27.457448233992114 22.749454047437997 80-81 22.812522743529442 27.19771414204607 27.306736054494785 22.683027059929707 82-83 22.798113240835626 27.174577048669356 27.398274085106323 22.629035625388696 84-85 22.72536570511931 27.311084897468785 27.256395210903534 22.707154186508372 86-87 22.788094183629777 27.286055302469258 27.216224311843153 22.70962620205781 88-89 22.82135867018295 27.254174056070646 26.987255001056727 22.93721227268968 90-91 22.844819822551873 27.12334729054714 27.191221916023668 22.84061097087732 92-93 22.846174824424317 26.928134891523115 27.3885570376556 22.837133246396967 94-95 22.85497946682668 26.77575422310724 27.446544371227276 22.9227219388388 96-97 22.87294426008249 26.635288072366674 27.435153692443098 23.056613975107737 98-99 22.705056434296424 26.854935986115606 27.17133416003039 23.26867341955758 100-101 23.171246860061117 25.938086312558305 27.731951242681745 23.158715584698836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3509.0 1 1989.5 2 465.0 3 466.5 4 400.5 5 391.0 6 635.0 7 1181.5 8 1793.5 9 2125.0 10 1985.0 11 1792.5 12 1970.0 13 2193.5 14 2359.5 15 2476.0 16 2631.5 17 2873.0 18 3136.5 19 3524.0 20 4056.0 21 4784.0 22 5731.0 23 7212.5 24 9215.5 25 11761.0 26 15184.0 27 18795.0 28 21547.5 29 24833.5 30 29557.0 31 34933.5 32 42068.0 33 46595.0 34 49579.5 35 54368.5 36 60605.5 37 67328.5 38 71129.5 39 74233.0 40 77854.0 41 81551.0 42 83993.0 43 86608.0 44 90873.0 45 95769.5 46 98633.0 47 102347.5 48 106646.0 49 105305.5 50 100872.0 51 96594.0 52 94567.0 53 94293.0 54 94790.0 55 90335.0 56 82432.0 57 76440.0 58 71181.5 59 63686.5 60 52608.5 61 43475.0 62 36243.5 63 27822.5 64 20336.0 65 15358.0 66 11435.0 67 8676.0 68 6628.5 69 5019.5 70 3797.5 71 2630.0 72 1875.5 73 1414.0 74 1014.5 75 743.0 76 575.5 77 397.5 78 271.5 79 184.5 80 134.0 81 99.5 82 61.5 83 44.0 84 36.0 85 27.0 86 19.0 87 10.0 88 7.5 89 5.5 90 5.5 91 6.0 92 7.0 93 4.5 94 2.0 95 2.5 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3483874376516742 2 0.30652223845624926 3 0.3051279548721772 4 0.30850358881256207 5 0.3188506406733069 6 0.21002313776916096 7 0.21127065466017272 8 0.21156418804629315 9 0.20939937932365504 10-11 0.21635245140738252 12-13 0.20451938677940304 14-15 0.20664750382877609 16-17 0.21415095101147935 18-19 0.20723457060101694 20-21 0.1980066147748562 22-23 0.19641052698782646 24-25 0.2011254070023857 26-27 0.19587849772548316 28-29 0.21194945061557618 30-31 0.21686613483309325 32-33 0.21998492706062273 34-35 0.21807696005083999 36-37 0.18929234237440623 38-39 0.17967912397896246 40-41 0.18643039185973215 42-43 0.20086856528953034 44-45 0.20675757884857127 46-47 0.21044509201170905 48-49 0.20758314149703494 50-51 0.21110554213047997 52-53 0.23814730532682374 54-55 0.19719939796302507 56-57 0.17813807370183024 58-59 0.17764273611275203 60-61 0.17129507663789795 62-63 0.17771611945928215 64-65 0.17500093563766825 66-67 0.17505597314756582 68-69 0.18395370391434107 70-71 0.17826649455825794 72-73 0.17415702715257206 74-75 0.1801928074046732 76-77 0.18677896275575012 78-79 0.18964091327042423 80-81 0.1783215320681555 82-83 0.1750192814743008 84-85 0.16909357624199478 86-87 0.1672773384153747 88-89 0.17388183960308418 90-91 0.1818806243748656 92-93 0.1706162806824945 94-95 0.17672544428112572 96-97 0.17500093563766825 98-99 0.18160543682537772 100-101 0.1549305903616845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2725414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.24071684865357 #Duplication Level Percentage of deduplicated Percentage of total 1 77.01522813973766 42.54376410701705 2 12.813194844907963 14.156201367083767 3 4.308112841919236 7.139497249575262 4 1.9256007917326297 4.254862723985813 5 1.0417090438935876 2.8772377166203658 6 0.6407101230987783 2.123597189529935 7 0.3999819977507029 1.546670459761376 8 0.30916266977743634 1.3662694001079316 9 0.20683315400333177 1.0283050525690804 >10 1.200208951569231 12.23171787686785 >50 0.09099492610000824 3.458707817126405 >100 0.04340359394855039 4.162734767776453 >500 0.00339458903567971 1.2720074902795002 >1k 0.0013312113865110442 1.3452871817760625 >5k 1.3312113865110442E-4 0.49313959992294054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7374 0.27056439865649773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6038 0.22154432317438746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3923 0.14394143421880126 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3826 0.14038234191209115 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2884 0.10581878569641164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.669167326505257E-5 0.0 0.0 0.0 0.0 3 3.669167326505257E-5 0.0 0.0 3.669167326505257E-5 0.0 4 3.669167326505257E-5 0.0 0.0 3.669167326505257E-5 0.0 5 3.669167326505257E-5 0.0 0.0 3.669167326505257E-5 0.0 6 7.338334653010515E-5 0.0 0.0 3.669167326505257E-5 0.0 7 1.1007501979515772E-4 0.0 0.0 3.669167326505257E-5 0.0 8 1.1007501979515772E-4 0.0 0.0 3.669167326505257E-5 0.0 9 1.1007501979515772E-4 0.0 0.0 7.338334653010515E-5 0.0 10-11 1.467666930602103E-4 0.0 0.0 9.172918316263144E-5 0.0 12-13 1.8345836632526288E-4 0.0 0.0 1.1007501979515772E-4 0.0 14-15 1.8345836632526288E-4 0.0 0.0 1.1007501979515772E-4 0.0 16-17 2.0180420295778917E-4 0.0 0.0 1.1007501979515772E-4 1.8345836632526287E-5 18-19 2.935333861204206E-4 0.0 0.0 1.28420856427684E-4 3.669167326505257E-5 20-21 3.302250593854732E-4 3.669167326505257E-5 0.0 1.651125296927366E-4 3.669167326505257E-5 22-23 4.0360840591557835E-4 3.669167326505257E-5 0.0 1.8345836632526288E-4 7.338334653010515E-5 24-25 4.403000791806309E-4 3.669167326505257E-5 0.0 2.2015003959031544E-4 7.338334653010515E-5 26-27 4.403000791806309E-4 3.669167326505257E-5 0.0 2.751875494878943E-4 7.338334653010515E-5 28-29 4.7699175244568347E-4 3.669167326505257E-5 0.0 5.870667722408412E-4 7.338334653010515E-5 30-31 4.7699175244568347E-4 3.669167326505257E-5 0.0 0.0013759377474394717 7.338334653010515E-5 32-33 4.7699175244568347E-4 3.669167326505257E-5 0.0 0.0021831545592706286 7.338334653010515E-5 34-35 4.953375890782098E-4 3.669167326505257E-5 0.0 0.003375633940384837 7.338334653010515E-5 36-37 5.503750989757886E-4 3.669167326505257E-5 0.0 0.0056321718461855705 7.338334653010515E-5 38-39 7.338334653010515E-4 3.669167326505257E-5 0.0 0.008677580727184935 7.338334653010515E-5 40-41 7.338334653010515E-4 3.669167326505257E-5 0.0 0.011557877078491562 7.338334653010515E-5 42-43 7.338334653010515E-4 7.338334653010515E-5 0.0 0.01408960253378019 7.338334653010515E-5 44-45 8.072168118311567E-4 7.338334653010515E-5 0.0 0.01660298215243629 7.338334653010515E-5 46-47 8.98945994993788E-4 7.338334653010515E-5 0.0 0.02071244955812218 7.338334653010515E-5 48-49 9.539835048913669E-4 7.338334653010515E-5 0.0 0.023849587622284174 7.338334653010515E-5 50-51 9.539835048913669E-4 7.338334653010515E-5 0.0 0.02757379245868701 7.338334653010515E-5 52-53 9.539835048913669E-4 7.338334653010515E-5 0.0 0.03626971902250447 7.338334653010515E-5 54-55 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.05175360514035666 7.338334653010515E-5 56-57 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.07219086714899095 7.338334653010515E-5 58-59 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.09866390940972639 1.1007501979515772E-4 60-61 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.13164972367500863 1.28420856427684E-4 62-63 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.15449029028250388 1.467666930602103E-4 64-65 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.18371520803811825 1.467666930602103E-4 66-67 9.539835048913669E-4 1.1007501979515772E-4 0.0 0.23251513348063818 1.467666930602103E-4 68-69 9.539835048913669E-4 1.28420856427684E-4 0.0 0.29839503282804003 1.467666930602103E-4 70-71 9.906751781564196E-4 1.467666930602103E-4 0.0 0.3631925278141229 1.467666930602103E-4 72-73 0.0010273668514214722 1.467666930602103E-4 0.0 0.4368694077303485 1.467666930602103E-4 74-75 0.0010273668514214722 1.467666930602103E-4 0.0 0.5110783169089173 1.467666930602103E-4 76-77 0.0010273668514214722 1.467666930602103E-4 0.0 0.5882592516219554 1.467666930602103E-4 78-79 0.0010273668514214722 1.467666930602103E-4 0.0 0.6688341661120109 1.467666930602103E-4 80-81 0.0011007501979515772 1.467666930602103E-4 0.0 0.7480514886912594 1.467666930602103E-4 82-83 0.0011007501979515772 1.467666930602103E-4 0.0 0.849375544412702 1.467666930602103E-4 84-85 0.0011190960345841034 1.467666930602103E-4 0.0 0.9884920235971489 1.467666930602103E-4 86-87 0.0011374418712166298 1.467666930602103E-4 0.0 1.153439440760193 1.467666930602103E-4 88-89 0.0011374418712166298 1.467666930602103E-4 0.0 1.3458689211987611 1.467666930602103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11125 0.0 31.858055 1 GGTATCA 7450 0.0 31.800653 1 ATCAACG 13080 0.0 26.82895 3 TCAACGC 13100 0.0 26.787497 4 GTACATG 10340 0.0 26.777216 1 TACATGG 10080 0.0 26.652811 2 TATCAAC 13395 0.0 26.233534 2 CAACGCA 13370 0.0 26.212423 5 AACGCAG 13760 0.0 25.477777 6 ACATGGG 10305 0.0 25.19415 3 CATGGGG 5390 0.0 22.583864 4 ACGCAGA 15650 0.0 22.249548 7 CGCAGAG 15670 0.0 22.190022 8 GAGTACT 7990 0.0 22.145584 12-13 GAGTACA 7485 0.0 21.037745 1 GCAGAGT 16550 0.0 20.550514 9 GTACTTT 8600 0.0 20.51918 14-15 AGAGTAC 12565 0.0 20.357615 10-11 AGTACTT 8555 0.0 20.26658 12-13 CATGGGA 5050 0.0 19.39648 4 >>END_MODULE