FastQCFastQC Report
Thu 26 May 2016
SRR937907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725414
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84520.3101180224362244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74310.2726558240326057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.1771840901969389No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32050.11759681281449351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86300.031.2275241
GTATCAA123350.030.2094081
ACCGTAT1056.907454E-527.1438968
GTACATG94500.025.550371
TACATGG95250.024.893232
TAAGGTG12700.024.6936865
ATCAACG149850.024.6065463
TCAACGC151350.024.394074
CAACGCA153850.023.9667935
TATCAAC154050.023.9356772
ACATGGG94750.023.8711553
AACGCAG156300.023.581166
GTAAGGT11050.022.3607714
GAGTACT100250.021.10928512-13
AGAGTAC141550.021.05785410-11
ACGCAGA177300.020.7636267
CGCAGAG179700.020.3277118
AGTACTT104900.020.01506412-13
GTACTTT105000.019.94856614-15
GCGACAG8150.019.816717
Produced by FastQC (version 0.11.5)