##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937907_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2725414 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.979969648647874 31.0 31.0 34.0 30.0 34.0 2 32.26111702662421 33.0 31.0 34.0 31.0 34.0 3 32.360151155017185 34.0 31.0 34.0 30.0 34.0 4 36.0308980580565 37.0 35.0 37.0 35.0 37.0 5 35.918911035167504 37.0 35.0 37.0 35.0 37.0 6 35.95215956181336 37.0 35.0 37.0 35.0 37.0 7 35.88926966692033 37.0 35.0 37.0 35.0 37.0 8 35.85133891584911 37.0 35.0 37.0 35.0 37.0 9 37.52856703605397 39.0 37.0 39.0 35.0 39.0 10-11 37.42836281020058 39.0 37.0 39.0 34.0 39.0 12-13 37.314209327463644 39.0 37.0 39.0 34.0 39.0 14-15 38.50570280331722 40.0 38.0 41.0 34.0 41.0 16-17 38.44949978241838 40.0 38.0 41.0 34.0 41.0 18-19 38.39565144965132 40.0 38.0 41.0 34.0 41.0 20-21 38.268328408087726 40.0 38.0 41.0 34.0 41.0 22-23 38.20793446426855 40.0 38.0 41.0 33.5 41.0 24-25 38.239435549975155 40.0 38.0 41.0 33.5 41.0 26-27 38.08573413066786 40.0 37.5 41.0 33.0 41.0 28-29 37.92656033175143 40.0 37.0 41.0 33.0 41.0 30-31 37.99548894223042 40.0 37.0 41.0 33.0 41.0 32-33 37.93590735205734 40.0 37.0 41.0 33.0 41.0 34-35 37.79429051879824 40.0 37.0 41.0 33.0 41.0 36-37 37.633499534382665 40.0 37.0 41.0 33.0 41.0 38-39 37.486247043568426 40.0 37.0 41.0 32.5 41.0 40-41 37.30535397557949 40.0 36.5 41.0 32.0 41.0 42-43 37.08270119695577 39.5 36.0 41.0 31.0 41.0 44-45 36.81988754735978 39.0 36.0 41.0 31.0 41.0 46-47 36.49913536071951 39.0 35.5 41.0 30.0 41.0 48-49 36.196067459842794 39.0 35.0 40.5 30.0 41.0 50-51 35.93159974961602 39.0 35.0 40.0 29.0 41.0 52-53 35.6748405930255 38.5 35.0 40.0 28.0 41.0 54-55 35.422306849528184 38.0 34.5 40.0 28.0 41.0 56-57 35.115486858143385 38.0 34.0 40.0 27.0 41.0 58-59 34.81617013048293 38.0 34.0 40.0 26.5 41.0 60-61 34.44323688070877 37.5 34.0 40.0 26.0 41.0 62-63 34.034995600668374 37.0 33.0 39.5 26.0 41.0 64-65 33.586797455359076 36.0 33.0 39.0 25.0 40.5 66-67 33.125969852653576 36.0 32.0 39.0 23.5 40.0 68-69 32.64502292128829 35.0 31.5 38.0 22.0 40.0 70-71 32.219504449599214 35.0 31.0 38.0 22.0 40.0 72-73 31.703551093521938 35.0 31.0 37.0 20.5 39.0 74-75 31.206549537061157 34.0 30.0 36.5 19.5 39.0 76-77 30.02151783178629 33.5 29.0 35.0 18.5 37.5 78-79 30.632523168957082 34.0 30.0 36.0 18.0 37.5 80-81 30.445204471687603 34.0 30.0 35.0 18.0 37.0 82-83 30.049457623685797 34.0 30.0 35.0 14.5 37.0 84-85 29.63933406080691 34.0 29.0 35.0 9.0 36.0 86-87 29.231915591539487 34.0 29.0 35.0 7.0 36.0 88-89 28.731929350916964 33.5 29.0 35.0 2.0 35.5 90-91 28.11760947144177 33.0 27.0 35.0 2.0 35.0 92-93 27.609157911421896 33.0 26.5 35.0 2.0 35.0 94-95 27.046508163530383 32.5 25.5 34.0 2.0 35.0 96-97 26.371511630893508 32.0 25.0 34.0 2.0 35.0 98-99 25.34075428540398 32.0 22.0 34.0 2.0 35.0 100-101 22.469766611604697 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3526920256521997 1101 2 0.28482178903129807 1101 3 0.2473684169184125 1101 4 0.16408740406753708 1101 5 0.06026356605064365 1101 6 0.09703246411590527 1101 7 0.21525128081281508 1101 8 0.1368030181309834 1101 9 0.18074421650125316 1101 10-11 0.2851853037683014 1101 12-13 0.33279556299666524 1101 14-15 0.3821861522343468 1101 16-17 0.2697574401723699 1101 18-19 0.37900277883618116 1101 20-21 0.42889729016466305 1101 22-23 0.37012508986492776 1101 24-25 0.3078591607362213 1101 26-27 0.23332454934210745 1101 28-29 0.19695695979463324 1101 30-31 0.2806384769584085 1101 32-33 0.2942350799684661 1101 34-35 0.379492400614005 1101 36-37 0.25677950230463154 1101 38-39 0.18019270441689628 1101 40-41 0.31360981090619333 1101 42-43 0.3369803337982873 1101 44-45 0.32297252630274187 1101 46-47 0.2267321490444303 1101 48-49 0.20539608027397094 1101 50-51 0.2568985804341537 1101 52-53 0.25671977464249096 1101 54-55 0.1504534514689837 1101 56-57 0.2838114541944279 1101 58-59 0.13056474463251533 1101 60-61 0.16921705423745692 1101 62-63 -0.009962440657105276 1101 64-65 0.16807287802426174 1101 66-67 0.09174470532708057 1101 68-69 0.18736748009850146 1101 70-71 0.10362651165857528 1101 72-73 -0.024544246330364672 1101 74-75 0.07452300406486145 1101 76-77 0.06502082499686779 1101 78-79 0.054437734989601694 1101 80-81 0.015122829880336752 1101 82-83 0.1193056086325548 1101 84-85 0.13393187885354507 1101 86-87 0.02279971358289501 1101 88-89 -0.0769723628918797 1101 90-91 -0.1092454019007505 1101 92-93 -0.03648215855800174 1101 94-95 -0.22176819227034628 1101 96-97 -0.09131313842253519 1101 98-99 -0.04554533941471561 1101 100-101 -0.2564355118915458 1102 1 0.3183238464410785 1102 2 0.21960379284536202 1102 3 0.22063715878924484 1102 4 0.15757120873392694 1102 5 0.07725583686229243 1102 6 0.07225968702864094 1102 7 0.11225134774923617 1102 8 0.1732378266468757 1102 9 0.18890849161258672 1102 10-11 0.24676097408409703 1102 12-13 0.299203255698103 1102 14-15 0.3818550138032677 1102 16-17 0.36875529689429243 1102 18-19 0.3071510548294967 1102 20-21 0.302055521310578 1102 22-23 0.29198180129149875 1102 24-25 0.1785643031598667 1102 26-27 0.2729288631978193 1102 28-29 0.1975236881915592 1102 30-31 0.1640345686586926 1102 32-33 0.2346178906945724 1102 34-35 0.2894770839850267 1102 36-37 0.1672814992413123 1102 38-39 0.20522010241430877 1102 40-41 0.2566528851518157 1102 42-43 0.27643108762055846 1102 44-45 0.2711876862217295 1102 46-47 0.14535596239942805 1102 48-49 0.1458384775429593 1102 50-51 0.0668380489064404 1102 52-53 0.11703284023887761 1102 54-55 0.1015485838784187 1102 56-57 0.14591552852272827 1102 58-59 0.06921849563164528 1102 60-61 -0.13237216086077552 1102 62-63 -0.18339819547029634 1102 64-65 -0.20118847196530254 1102 66-67 -0.23067689702592986 1102 68-69 -0.10680012608432321 1102 70-71 -0.11414799658838604 1102 72-73 -0.17652379265889806 1102 74-75 -0.10177159232818767 1102 76-77 -0.21499287728303784 1102 78-79 -0.11967886826245788 1102 80-81 -0.025897714352172585 1102 82-83 -0.09889978644328679 1102 84-85 -0.12068981878543639 1102 86-87 -0.3451891224714281 1102 88-89 -0.311225897069491 1102 90-91 -0.19922974402725657 1102 92-93 -0.22055831120979974 1102 94-95 -0.37705531672090586 1102 96-97 -0.276337097725186 1102 98-99 0.0022901203532121883 1102 100-101 0.028017781577428735 1103 1 0.3217127861662128 1103 2 0.2228366867954037 1103 3 0.24819297012116692 1103 4 0.08425191535913257 1103 5 0.0714394490598309 1103 6 0.11951197060250962 1103 7 0.13291730118267964 1103 8 0.09482632270265867 1103 9 0.1843076379797779 1103 10-11 0.22195300401493512 1103 12-13 0.24449054845370455 1103 14-15 0.26368754048397136 1103 16-17 0.22874366212130326 1103 18-19 0.16085508218369426 1103 20-21 0.16129368086925666 1103 22-23 0.17107996906442935 1103 24-25 0.12577395502798794 1103 26-27 0.059119061775426474 1103 28-29 0.17127485385349672 1103 30-31 0.1732191108890433 1103 32-33 0.2016973504516102 1103 34-35 0.23607940569649344 1103 36-37 0.254657832964547 1103 38-39 0.2854104033579503 1103 40-41 0.27971545201743453 1103 42-43 0.24527842117277032 1103 44-45 0.15173246074807167 1103 46-47 0.19328654574846382 1103 48-49 0.08512599750121552 1103 50-51 0.031215732113544448 1103 52-53 0.08847034640267992 1103 54-55 -0.041277055565963394 1103 56-57 0.12396550561521025 1103 58-59 0.012871614612173232 1103 60-61 0.1475595410243855 1103 62-63 0.00886443081755317 1103 64-65 0.08165760323377924 1103 66-67 0.019611787657993318 1103 68-69 -0.03106948855618441 1103 70-71 -0.03679878986269358 1103 72-73 0.0095177879225119 1103 74-75 -0.018474232747539787 1103 76-77 -0.036313973298629065 1103 78-79 0.1154361125064618 1103 80-81 0.09076054664874178 1103 82-83 -0.07733906653546896 1103 84-85 -0.03105325563745609 1103 86-87 -0.10490709157935285 1103 88-89 -0.14423475922114903 1103 90-91 -0.13367915835625155 1103 92-93 -0.11293572476980529 1103 94-95 -0.21765374602655285 1103 96-97 -0.4750721282539807 1103 98-99 -0.475662983522092 1103 100-101 -0.200198602729607 1104 1 0.2622548769958364 1104 2 0.24601019298646776 1104 3 0.18544201707594965 1104 4 0.11741146242703593 1104 5 0.08087995412063975 1104 6 0.10142949495214282 1104 7 0.10999380735486852 1104 8 0.07016850995367463 1104 9 0.06879989548033194 1104 10-11 0.1329780475371507 1104 12-13 0.1231535043141605 1104 14-15 0.23508555276026044 1104 16-17 0.24605256870125203 1104 18-19 0.2386002669073548 1104 20-21 0.21539866169367627 1104 22-23 0.06616155814444369 1104 24-25 0.04817079835014937 1104 26-27 0.1308077477663545 1104 28-29 0.05450217780381905 1104 30-31 0.1348201145698269 1104 32-33 0.12197494181840796 1104 34-35 0.13890277751635693 1104 36-37 0.09391762196988651 1104 38-39 0.053506675677198245 1104 40-41 -0.03338495691344434 1104 42-43 0.1789450525613745 1104 44-45 0.10885569880594659 1104 46-47 -0.010537974479831291 1104 48-49 -0.06875255072131381 1104 50-51 -0.023295026062974955 1104 52-53 0.052968471369688075 1104 54-55 -0.012620418699299307 1104 56-57 0.15951301118942496 1104 58-59 0.10537117204371071 1104 60-61 0.11094754181659283 1104 62-63 0.009366247319228194 1104 64-65 0.12877639291114917 1104 66-67 0.1447648948469933 1104 68-69 0.16263801202973127 1104 70-71 0.09251977653560317 1104 72-73 0.10589970408511462 1104 74-75 0.08429004632694159 1104 76-77 0.09120320206097787 1104 78-79 0.0730044275186934 1104 80-81 0.223458162130445 1104 82-83 0.15738867800422085 1104 84-85 0.2616517347908811 1104 86-87 0.15909354103673579 1104 88-89 0.08001659530116001 1104 90-91 0.149251459569669 1104 92-93 0.36297105083998105 1104 94-95 0.38036644990764756 1104 96-97 0.2577077774890846 1104 98-99 0.1861577276310875 1104 100-101 0.14930034525945857 1105 1 0.3067064906688479 1105 2 0.22402726543860751 1105 3 0.164602469306395 1105 4 0.1043004552340463 1105 5 0.05811343396934632 1105 6 0.09799947034526468 1105 7 0.13356789827272308 1105 8 0.0688148336538319 1105 9 0.14391659685605873 1105 10-11 0.16782669370023484 1105 12-13 0.1842193462849906 1105 14-15 0.2354674824494012 1105 16-17 0.19780288282666447 1105 18-19 0.25609365627692426 1105 20-21 0.09999887573033561 1105 22-23 0.12771189668397653 1105 24-25 0.11143322362776331 1105 26-27 0.11225404172898834 1105 28-29 0.03519166816345631 1105 30-31 0.1202164954045557 1105 32-33 0.22103039722912143 1105 34-35 0.2818559969232055 1105 36-37 0.17953222703462046 1105 38-39 0.04947868845698622 1105 40-41 0.14705140894238156 1105 42-43 0.08469681730829137 1105 44-45 -0.004702931359453544 1105 46-47 0.023439312119101885 1105 48-49 0.2117799687159163 1105 50-51 0.20644325400747476 1105 52-53 0.20677880800014492 1105 54-55 0.029252509885701272 1105 56-57 0.012479660662179981 1105 58-59 0.09892792984728516 1105 60-61 0.06597076047999195 1105 62-63 -0.0604600567027731 1105 64-65 -0.01503195204031016 1105 66-67 0.019992744760671144 1105 68-69 -0.08106513876974475 1105 70-71 -0.1711135301196336 1105 72-73 -0.08648071257046297 1105 74-75 -0.0510078290012963 1105 76-77 -0.2121952493856547 1105 78-79 -0.0907774404926478 1105 80-81 -0.14568565912141196 1105 82-83 -0.12120946909183417 1105 84-85 -0.09480861335828195 1105 86-87 -0.16086791263194655 1105 88-89 -0.15179929508490275 1105 90-91 -0.021072775676074684 1105 92-93 -0.1453291222617068 1105 94-95 -0.22905147272525994 1105 96-97 -0.1998734178487318 1105 98-99 -0.11161205079691783 1105 100-101 -0.3333597141421407 1106 1 0.06785970638263805 1106 2 0.07432711512937118 1106 3 0.11449150791434448 1106 4 0.0789588348575947 1106 5 -0.022368287104043816 1106 6 -0.05419806842249386 1106 7 -0.08751784135962026 1106 8 -0.03670134299353833 1106 9 -9.12947063035574E-4 1106 10-11 -0.13924934898274444 1106 12-13 -0.28372811723497193 1106 14-15 -0.060055692476815636 1106 16-17 -0.032288015590197006 1106 18-19 0.053909909057367145 1106 20-21 0.08993745208844928 1106 22-23 0.08693212907157033 1106 24-25 0.02374553510056643 1106 26-27 0.10666182519423018 1106 28-29 0.07144218185202789 1106 30-31 0.056662734924607605 1106 32-33 -0.03412949387355724 1106 34-35 -0.2631456850865632 1106 36-37 0.14575813492385947 1106 38-39 0.16842112365301887 1106 40-41 0.019891587263380472 1106 42-43 -0.1829982399126564 1106 44-45 -0.044043905024118146 1106 46-47 0.03403236266979093 1106 48-49 0.08950700275961054 1106 50-51 0.23210374569480763 1106 52-53 0.4341984675725499 1106 54-55 0.20305426430109463 1106 56-57 0.3012219716410911 1106 58-59 0.2658915108414064 1106 60-61 0.2637261277684928 1106 62-63 0.13889333634423906 1106 64-65 -0.13324444858077555 1106 66-67 -0.2785757531067645 1106 68-69 0.1734365670024829 1106 70-71 0.16957865417272444 1106 72-73 0.3721894304050295 1106 74-75 0.2060773459230596 1106 76-77 0.1622593787504094 1106 78-79 0.014049741157887041 1106 80-81 -0.2543482656681135 1106 82-83 -0.459880620807251 1106 84-85 -0.45910391416779106 1106 86-87 -0.11866129054552843 1106 88-89 -0.21579970836542017 1106 90-91 -0.4374024579765816 1106 92-93 -0.38887429330952017 1106 94-95 -0.3553446050933715 1106 96-97 -0.37146072021258547 1106 98-99 -0.7619557678614406 1106 100-101 -1.0560980516641862 1107 1 0.13906069148569244 1107 2 0.07259981399381843 1107 3 0.11840913263113606 1107 4 -0.025077148601042154 1107 5 -0.0462896127681276 1107 6 -0.046900529921849454 1107 7 0.01716517296613773 1107 8 -0.03674393967327916 1107 9 -0.01060028627932752 1107 10-11 0.03675389806171836 1107 12-13 0.07775827370304 1107 14-15 -0.021051621319351455 1107 16-17 -0.015098004269177068 1107 18-19 0.10805525285355344 1107 20-21 0.043171607884019636 1107 22-23 -0.06371166375527082 1107 24-25 0.006810584386307994 1107 26-27 -0.026123627104581715 1107 28-29 -0.0824824788203955 1107 30-31 0.004617903823906033 1107 32-33 -0.038232165955200514 1107 34-35 -0.10519154012370535 1107 36-37 -0.11357206749580229 1107 38-39 -0.10700929617131294 1107 40-41 -0.1860290164504761 1107 42-43 -0.08821191189998245 1107 44-45 -0.1095382625801804 1107 46-47 -0.23901535588552747 1107 48-49 -0.22745206298553455 1107 50-51 -0.18217009000461104 1107 52-53 -0.14614902898363624 1107 54-55 -0.28654516183274836 1107 56-57 -0.36736345034366735 1107 58-59 -0.3300212627022887 1107 60-61 -0.2481219251418949 1107 62-63 -0.3550886994007243 1107 64-65 -0.22268448925195372 1107 66-67 -0.30744371921458225 1107 68-69 -0.11027094179938501 1107 70-71 -0.17349615887228964 1107 72-73 -0.17782813351048077 1107 74-75 -0.38991352153350434 1107 76-77 -0.222001228890651 1107 78-79 -0.06488288763329209 1107 80-81 -0.11747041528358082 1107 82-83 -0.2664320132396085 1107 84-85 -0.10385458910582912 1107 86-87 -0.25024601285962333 1107 88-89 -0.26371762537161914 1107 90-91 -0.28081331149513744 1107 92-93 -0.19259554617830332 1107 94-95 -0.2001967617147855 1107 96-97 -0.16325928940281287 1107 98-99 0.014242835175249269 1107 100-101 0.02596323976324655 1108 1 0.11902323178739138 1108 2 0.041802090530488556 1108 3 0.01269601004677412 1108 4 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2316 92-93 0.40446346144703327 2316 94-95 0.4350306944311768 2316 96-97 0.3070545327533942 2316 98-99 0.16435650808068303 2316 100-101 0.28545819925361116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 30.0 5 55.0 6 141.0 7 321.0 8 783.0 9 1568.0 10 2414.0 11 3849.0 12 5983.0 13 8129.0 14 10678.0 15 14097.0 16 18139.0 17 23416.0 18 21717.0 19 16189.0 20 15283.0 21 15717.0 22 17087.0 23 19098.0 24 22164.0 25 26598.0 26 32905.0 27 40252.0 28 49877.0 29 61613.0 30 76574.0 31 94504.0 32 118886.0 33 153178.0 34 205574.0 35 287106.0 36 421067.0 37 564852.0 38 353134.0 39 22425.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89067437031687 19.169802513227804 14.545557299935192 23.393965816520133 2 16.04941487788644 20.092250204922994 39.55736633039971 24.30096858679085 3 19.97755203429644 23.227260152035615 29.419530390612213 27.375657423055728 4 13.377673997418373 16.161911548117093 35.74227621931934 34.71813823514519 5 13.797541258311036 37.37131727429202 34.099467777973956 14.731673689422992 6 33.7539911367594 35.61844182204979 16.289451804386417 14.338115236804391 7 29.797968308667965 32.13548473736467 19.709812894481352 18.35673405948601 8 26.786462533765516 35.61924903886162 18.65731224687332 18.93697618049955 9 26.178261357724004 16.746446594902643 19.84421449365124 37.231077553722116 10-11 25.525498144502084 26.474564965175933 26.50751408776795 21.492422802554035 12-13 26.18042577924191 23.501995553531728 27.50031280856593 22.81726585866043 14-15 22.718823635601783 25.197621352205573 25.562465005316625 26.52109000687602 16-17 22.42606077461993 28.499871946060306 26.391091408497942 22.682975870821828 18-19 22.420685444486598 26.9549873890719 28.64080466307137 21.983522503370132 20-21 22.812864394180114 25.975925125503867 29.087874355969408 22.12333612434661 22-23 22.634104602241027 25.881462192437404 28.991885779723013 22.49254742559855 24-25 22.597411508252247 26.11635058878308 29.030940121108408 22.255297781856267 26-27 22.222986639514563 26.371405900995732 29.010626929198143 22.394980530291562 28-29 22.220085932655508 26.81175905099821 28.86238299839656 22.105772017949725 30-31 22.34618300192191 26.280667817806762 28.816245898788225 22.556903281483105 32-33 22.12761070428199 26.293344790919836 28.983871808099614 22.595172696698558 34-35 22.714897626562422 26.372507075989187 28.74007398508997 22.172521312358416 36-37 22.786886689508457 26.091320437922462 28.714169663764842 22.40762320880424 38-39 22.360878017064564 26.535289684429596 28.731543171055847 22.372289127449996 40-41 22.72609525207309 26.374770675864095 28.578465546341818 22.320668525720997 42-43 22.42361359570662 26.372294848860143 28.44271363698913 22.761377918444108 44-45 22.53886932407333 26.49771741100618 28.575163259600195 22.38825000532029 46-47 22.946220280661947 26.881438930012102 27.921005762794206 22.25133502653175 48-49 22.827944671892052 26.9360361398305 27.79546520271783 22.440553985559625 50-51 22.785143835028364 27.095571535186945 27.813334047597905 22.305950582186778 52-53 23.113717769116914 26.888887339684906 27.63947055383145 22.35792433736673 54-55 22.738262731430734 27.023672213782106 27.727914748989193 22.51015030579797 56-57 22.84539156252958 27.161029480291806 27.57590222989975 22.417676727278867 58-59 23.02567609911742 26.82267721527812 27.543521828243346 22.60812485736112 60-61 22.68481045448508 27.067814284361937 27.879874397064082 22.367500864088903 62-63 22.64815547289329 27.10474445350321 27.96318284121238 22.283917232391115 64-65 22.53953804432576 27.13663947445763 27.80389614344688 22.519926337769725 66-67 22.55429817268129 27.105203099419022 27.589918449087 22.750580278812688 68-69 22.763550785311885 26.88696102683849 27.826451320790163 22.523036867059464 70-71 22.739701197689598 26.983992156787924 27.595312125056964 22.680994520465514 72-73 22.7184383730325 27.002448068440245 27.5860841692308 22.693029389296452 74-75 22.55868282763646 27.44052830138834 27.362430074843676 22.638358796131524 76-77 22.782868951285934 27.06467714629777 27.535834922694313 22.61661897972198 78-79 22.762486726787195 27.149288144846984 27.473899378222903 22.614325750142914 80-81 22.88079902723036 27.28222207708627 27.329444260578395 22.507534635104978 82-83 22.82705969075331 27.340493880341104 27.3468782713124 22.48556815759319 84-85 22.73132549085221 27.424302298403948 27.289918995763212 22.55445321498063 86-87 22.8177076950511 27.369768409496686 27.23138576377754 22.581138131674674 88-89 22.77637452511802 27.43163057062156 27.034865161769915 22.7571297424905 90-91 22.82050138830099 27.286513288443132 27.178498646009764 22.71448667724612 92-93 22.792467437877644 27.088548608582148 27.42077903255249 22.69820492098772 94-95 22.728143320611107 26.96973744172445 27.45107715745204 22.851042080212398 96-97 22.80572786373006 26.83882155151474 27.42717253231986 22.928278052435335 98-99 22.596674328610618 27.076627450649443 27.140086619288468 23.18661160145147 100-101 22.665468838390986 26.65268833363334 27.63197900571079 23.04986382226489 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 444.0 1 326.5 2 286.0 3 376.0 4 310.0 5 280.5 6 564.5 7 1179.0 8 1894.5 9 2281.0 10 2092.0 11 1878.5 12 2029.5 13 2283.0 14 2544.0 15 2748.0 16 2897.5 17 3042.0 18 3330.0 19 3780.0 20 4270.5 21 4954.0 22 5975.5 23 7437.5 24 9410.0 25 11985.5 26 15256.0 27 18883.5 28 21681.0 29 25142.5 30 29920.5 31 35129.0 32 42442.5 33 47164.0 34 50107.5 35 55182.0 36 61200.0 37 67780.5 38 71864.0 39 74754.5 40 78037.0 41 81651.5 42 84212.5 43 87051.0 44 91532.0 45 95924.5 46 98238.5 47 102696.5 48 108099.0 49 106218.5 50 101250.0 51 97361.0 52 95018.0 53 94814.5 54 95858.5 55 90782.5 56 82063.0 57 75692.0 58 70629.5 59 62876.0 60 51282.0 61 42283.0 62 35407.5 63 27066.0 64 19336.0 65 14225.0 66 10448.5 67 8119.5 68 6227.5 69 4658.0 70 3382.0 71 2213.0 72 1578.0 73 1246.0 74 955.0 75 657.0 76 477.0 77 325.5 78 200.0 79 133.5 80 96.5 81 75.5 82 52.5 83 36.5 84 20.5 85 10.5 86 7.0 87 1.0 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017318469781104816 2 0.0 3 0.0 4 0.0 5 0.04311271608643678 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038526256928305205 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.953375890782098E-4 24-25 1.651125296927366E-4 26-27 9.723293415238932E-4 28-29 8.806001583612619E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.13683425710736E-4 42-43 0.010603893573600193 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.28420856427684E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.669167326505257E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.237584455058938E-4 84-85 3.669167326505257E-5 86-87 0.0 88-89 0.0 90-91 0.011759681281449351 92-93 0.001706162806824945 94-95 0.0 96-97 0.0 98-99 0.0014493210939695769 100-101 0.017703732350387868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2725414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.653940376338596 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77143630265485 39.370107489331865 2 14.041223055606114 14.7865144316151 3 4.677899329062268 7.389294970768769 4 2.1674490701119593 4.564989364257025 5 1.1288939572135572 2.972035755716579 6 0.6986787632868166 2.2072913966590813 7 0.458110645499284 1.6884931429719696 8 0.3309097315113939 1.3938961018360916 9 0.23983880501695318 1.1365612325365455 >10 1.3271560424038944 12.965421976244532 >50 0.10555664146831502 3.803149677753169 >100 0.04789697944555629 4.513364301498194 >500 0.003346936373427313 1.242674188348739 >1k 0.0014642846633569858 1.3830702676417854 >5k 1.3945568222447483E-4 0.5831357028205909 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8452 0.3101180224362244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7431 0.2726558240326057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4829 0.1771840901969389 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3205 0.11759681281449351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.669167326505257E-5 0.0 0.0 0.0 0.0 3 3.669167326505257E-5 0.0 0.0 0.0 0.0 4 3.669167326505257E-5 0.0 0.0 0.0 0.0 5 1.1007501979515772E-4 0.0 0.0 0.0 0.0 6 1.1007501979515772E-4 0.0 0.0 0.0 0.0 7 1.1007501979515772E-4 0.0 0.0 0.0 0.0 8 1.467666930602103E-4 0.0 0.0 0.0 0.0 9 1.467666930602103E-4 0.0 0.0 0.0 0.0 10-11 1.8345836632526288E-4 0.0 0.0 0.0 0.0 12-13 2.2015003959031544E-4 0.0 0.0 0.0 0.0 14-15 2.2015003959031544E-4 0.0 0.0 0.0 0.0 16-17 2.2015003959031544E-4 0.0 0.0 7.338334653010515E-5 0.0 18-19 3.302250593854732E-4 0.0 0.0 9.172918316263144E-5 0.0 20-21 4.0360840591557835E-4 0.0 0.0 1.28420856427684E-4 0.0 22-23 4.0360840591557835E-4 0.0 0.0 1.467666930602103E-4 3.669167326505257E-5 24-25 4.403000791806309E-4 0.0 0.0 1.8345836632526288E-4 3.669167326505257E-5 26-27 4.7699175244568347E-4 0.0 0.0 2.3849587622284176E-4 3.669167326505257E-5 28-29 4.953375890782098E-4 0.0 0.0 5.320292623432624E-4 3.669167326505257E-5 30-31 5.136834257107361E-4 0.0 0.0 0.0013209002375418927 3.669167326505257E-5 32-33 5.503750989757886E-4 0.0 0.0 0.002219846232535681 3.669167326505257E-5 34-35 5.687209356083149E-4 0.0 0.0 0.003320596430487258 3.669167326505257E-5 36-37 7.338334653010515E-4 0.0 0.0 0.005503750989757887 3.669167326505257E-5 38-39 7.705251385661041E-4 0.0 0.0 0.008604197380654828 3.669167326505257E-5 40-41 7.705251385661041E-4 0.0 0.0 0.011649606261654191 3.669167326505257E-5 42-43 8.622543217287355E-4 0.0 0.0 0.014273060900105453 5.503750989757886E-5 44-45 9.539835048913669E-4 0.0 0.0 0.01685982386529166 7.338334653010515E-5 46-47 9.906751781564196E-4 0.0 0.0 0.020969291270977547 7.338334653010515E-5 48-49 9.906751781564196E-4 0.0 0.0 0.024161466845037123 9.172918316263144E-5 50-51 9.906751781564196E-4 0.0 0.0 0.027904017518072484 1.1007501979515772E-4 52-53 9.906751781564196E-4 0.0 0.0 0.036618289918522465 1.1007501979515772E-4 54-55 0.0010640585246865246 0.0 0.0 0.05232232607596497 1.1007501979515772E-4 56-57 0.0011374418712166298 0.0 0.0 0.07283297143112935 1.1007501979515772E-4 58-59 0.0011374418712166298 0.0 0.0 0.09963623875125027 1.28420856427684E-4 60-61 0.0011374418712166298 0.0 0.0 0.13297062391255055 1.467666930602103E-4 62-63 0.0011374418712166298 0.0 0.0 0.15617810725269626 1.467666930602103E-4 64-65 0.0011374418712166298 0.0 0.0 0.18525625831525044 1.467666930602103E-4 66-67 0.001210825217746735 0.0 0.0 0.23431302547062574 1.467666930602103E-4 68-69 0.0012475168910117875 0.0 0.0 0.30081668326353356 1.467666930602103E-4 70-71 0.0012658627276443137 0.0 0.0 0.36656816175450774 1.467666930602103E-4 72-73 0.00128420856427684 0.0 0.0 0.44094218346276937 1.467666930602103E-4 74-75 0.00128420856427684 0.0 0.0 0.5157014677403139 1.467666930602103E-4 76-77 0.00128420856427684 0.0 0.0 0.593542852572123 1.467666930602103E-4 78-79 0.00128420856427684 0.0 0.0 0.675603779829413 1.467666930602103E-4 80-81 0.0013942835840719979 0.0 0.0 0.7555365900373301 1.467666930602103E-4 82-83 0.0013942835840719979 0.0 0.0 0.8585301168923327 1.467666930602103E-4 84-85 0.0014309752573370505 0.0 0.0 1.000380125735026 1.467666930602103E-4 86-87 0.001467666930602103 0.0 0.0 1.167749193333563 1.467666930602103E-4 88-89 0.001467666930602103 0.0 0.0 1.3649118996233232 1.467666930602103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8630 0.0 31.227524 1 GTATCAA 12335 0.0 30.209408 1 ACCGTAT 105 6.907454E-5 27.143896 8 GTACATG 9450 0.0 25.55037 1 TACATGG 9525 0.0 24.89323 2 TAAGGTG 1270 0.0 24.693686 5 ATCAACG 14985 0.0 24.606546 3 TCAACGC 15135 0.0 24.39407 4 CAACGCA 15385 0.0 23.966793 5 TATCAAC 15405 0.0 23.935677 2 ACATGGG 9475 0.0 23.871155 3 AACGCAG 15630 0.0 23.58116 6 GTAAGGT 1105 0.0 22.360771 4 GAGTACT 10025 0.0 21.109285 12-13 AGAGTAC 14155 0.0 21.057854 10-11 ACGCAGA 17730 0.0 20.763626 7 CGCAGAG 17970 0.0 20.327711 8 AGTACTT 10490 0.0 20.015064 12-13 GTACTTT 10500 0.0 19.948566 14-15 GCGACAG 815 0.0 19.81671 7 >>END_MODULE