FastQCFastQC Report
Thu 26 May 2016
SRR937906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629793
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73870.2808966333091616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.22176650405564238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.13932655536006067No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36030.13700698115783258No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26870.10217534231781741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70500.032.917481
GTATCAA105600.031.4781421
ATCAACG127100.025.7295173
GTACATG90400.025.6712631
TCAACGC127850.025.6191794
TATCAAC129250.025.4500792
CAACGCA129300.025.2201395
TACATGG90100.025.1128332
AACGCAG133100.024.5565436
ACATGGG93850.023.1457183
GTACACG4250.022.3788071
ACGCAGA150950.021.4324047
CGCAGAG153300.021.1038558
GAGTACT84100.020.89919312-13
TAAGGTG11250.020.704535
CATGGGG48150.020.53594
GTACTTT86450.020.05633714-15
AGAGTAC124750.019.9146410-11
GAGTACA69550.019.8288141
GTATCGA1200.004733326419.814571
Produced by FastQC (version 0.11.5)