##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937906_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2629793 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.284355080418877 31.0 31.0 34.0 28.0 34.0 2 31.40932004914455 33.0 31.0 34.0 28.0 34.0 3 31.457265647904606 33.0 31.0 34.0 28.0 34.0 4 35.05950582422267 37.0 35.0 37.0 32.0 37.0 5 35.027324584102246 37.0 35.0 37.0 32.0 37.0 6 35.09643914939313 37.0 35.0 37.0 32.0 37.0 7 35.00756637499605 37.0 35.0 37.0 32.0 37.0 8 34.9853125322031 37.0 35.0 37.0 32.0 37.0 9 36.53279630754208 39.0 37.0 39.0 32.0 39.0 10-11 36.40282238944282 39.0 37.0 39.0 32.0 39.0 12-13 36.25166125242557 39.0 37.0 39.0 32.0 39.0 14-15 37.31979361113213 40.0 37.0 41.0 32.0 41.0 16-17 37.23662736953061 40.0 37.0 41.0 31.5 41.0 18-19 37.14889156675069 40.0 37.0 41.0 31.0 41.0 20-21 36.958314779908534 39.0 36.0 41.0 31.0 41.0 22-23 36.77976289388556 39.0 36.0 41.0 30.0 41.0 24-25 36.60975331518488 39.0 36.0 41.0 30.0 41.0 26-27 36.70337551282553 39.0 36.0 41.0 30.0 41.0 28-29 36.634400882502916 39.0 36.0 41.0 30.0 41.0 30-31 36.41678926820476 39.0 36.0 41.0 30.0 41.0 32-33 36.17140341464138 39.0 35.0 40.0 29.5 41.0 34-35 35.88570374170134 38.5 35.0 40.0 28.5 41.0 36-37 35.886823031318436 39.0 35.0 40.0 28.0 41.0 38-39 35.80999512128901 39.0 35.0 40.0 27.5 41.0 40-41 35.598361163787416 39.0 35.0 40.0 27.0 41.0 42-43 35.33201947833917 38.0 35.0 40.0 26.0 41.0 44-45 35.11167551970821 38.0 34.5 40.0 25.5 41.0 46-47 34.77043858585067 38.0 34.0 40.0 24.0 41.0 48-49 34.43613033421262 38.0 33.5 40.0 23.5 41.0 50-51 33.52673214203551 37.5 32.5 39.5 22.5 40.5 52-53 33.61923543031713 37.5 33.0 39.5 22.0 40.5 54-55 33.88805563783917 38.0 33.0 40.0 21.5 41.0 56-57 33.56795230651234 38.0 33.0 40.0 19.5 41.0 58-59 33.29327897671033 37.5 32.5 40.0 18.5 41.0 60-61 32.927013266823664 37.0 32.0 40.0 17.0 41.0 62-63 32.515325921089605 36.0 31.5 39.5 13.5 41.0 64-65 32.10213617573703 36.0 31.0 39.0 9.5 41.0 66-67 31.651207718630324 35.0 31.0 39.0 8.0 40.0 68-69 31.12907688932171 35.0 30.0 38.0 6.5 40.0 70-71 30.648886243137767 35.0 29.5 37.5 2.0 40.0 72-73 30.146736073903917 34.5 29.0 37.0 2.0 39.0 74-75 29.628755000868892 34.0 29.0 36.5 2.0 39.0 76-77 29.14582801764245 34.0 28.5 36.0 2.0 39.0 78-79 28.578365483519043 34.0 27.5 35.5 2.0 37.5 80-81 28.020910391045987 33.5 26.0 35.0 2.0 37.0 82-83 27.469309561627092 33.0 26.0 35.0 2.0 36.5 84-85 26.96274839882835 33.0 25.0 35.0 2.0 36.0 86-87 26.396863365291487 32.5 24.5 35.0 2.0 36.0 88-89 25.849063785628758 32.0 22.5 35.0 2.0 35.0 90-91 25.277609112200086 32.0 19.5 34.0 2.0 35.0 92-93 24.65194751069761 31.0 17.5 34.0 2.0 35.0 94-95 23.938339253317658 31.0 6.0 34.0 2.0 35.0 96-97 23.07287151498236 31.0 2.0 34.0 2.0 35.0 98-99 22.035639877359166 30.0 2.0 34.0 2.0 35.0 100-101 19.54630250365713 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3534139981572153 1101 2 0.34943822290978943 1101 3 0.3879430653797904 1101 4 0.33055447991476683 1101 5 0.3168670009783341 1101 6 0.3187845578031343 1101 7 0.3626296782561411 1101 8 0.4092009057578352 1101 9 0.5173232886524275 1101 10-11 0.4729686504505324 1101 12-13 0.5690472900071981 1101 14-15 0.6483830119971188 1101 16-17 0.6539389375897358 1101 18-19 0.7060726029623936 1101 20-21 0.8193243262481644 1101 22-23 0.7792338443382434 1101 24-25 0.7564837357066025 1101 26-27 0.7564949448838405 1101 28-29 0.7119987374464287 1101 30-31 0.6849175950063113 1101 32-33 0.8040108842026967 1101 34-35 0.7664659670510545 1101 36-37 0.7748358296063955 1101 38-39 0.6707130964233556 1101 40-41 0.8296361307507425 1101 42-43 0.836232896797334 1101 44-45 1.0325362591981673 1101 46-47 1.134459880304739 1101 48-49 1.1265760345816602 1101 50-51 1.1005745556024564 1101 52-53 1.05384893757671 1101 54-55 1.219580098409061 1101 56-57 1.3303961484030111 1101 58-59 1.384782133724194 1101 60-61 1.4908803263964145 1101 62-63 1.5053991753181108 1101 64-65 1.6359391687046951 1101 66-67 1.5153688614094314 1101 68-69 1.59735413306759 1101 70-71 1.6900914867532606 1101 72-73 1.7059085533616702 1101 74-75 1.5306484788078656 1101 76-77 1.4357112657822135 1101 78-79 1.6899992167696887 1101 80-81 1.9115997498329662 1101 82-83 1.8909170404421864 1101 84-85 1.9820653454898114 1101 86-87 2.112264097986703 1101 88-89 2.281671439486086 1101 90-91 2.012718032322006 1101 92-93 2.0943727157190573 1101 94-95 2.134324289610589 1101 96-97 2.366774512254871 1101 98-99 2.6065860033269495 1101 100-101 2.5999915763235038 1102 1 0.2593770053578801 1102 2 0.2820439169918316 1102 3 0.3168473836041876 1102 4 0.2546146262050897 1102 5 0.23909088498938758 1102 6 0.34970551714635434 1102 7 0.22595822805270416 1102 8 0.33333376249703406 1102 9 0.33261235538147105 1102 10-11 0.2752750854028889 1102 12-13 0.42155181565043875 1102 14-15 0.5248523582033968 1102 16-17 0.538128658557568 1102 18-19 0.4346021906812041 1102 20-21 0.49192146585533436 1102 22-23 0.5387816836735126 1102 24-25 0.5760994622780444 1102 26-27 0.5828744898044107 1102 28-29 0.6327243592630865 1102 30-31 0.5528210163822536 1102 32-33 0.37767834231785713 1102 34-35 0.6847880686469594 1102 36-37 0.8122424445494545 1102 38-39 0.7505267868952288 1102 40-41 0.8317449278352882 1102 42-43 0.8308626091330567 1102 44-45 0.7702929581334317 1102 46-47 0.7597994976148996 1102 48-49 0.9846004735726765 1102 50-51 0.8914257957166782 1102 52-53 0.8822978192848296 1102 54-55 0.8844478067930694 1102 56-57 1.167211195274085 1102 58-59 1.109643272292196 1102 60-61 0.9925709737108903 1102 62-63 1.0872318098376255 1102 64-65 1.0803923964793682 1102 66-67 0.9446124734989318 1102 68-69 1.0509838852517532 1102 70-71 1.0412194302326583 1102 72-73 0.8643937204301011 1102 74-75 0.8157231195768482 1102 76-77 0.9043847710261659 1102 78-79 0.9915604343100348 1102 80-81 1.0559277914326586 1102 82-83 1.0285478933745686 1102 84-85 1.1550224147567327 1102 86-87 1.2937512543716068 1102 88-89 1.4571597897713922 1102 90-91 1.778000552523089 1102 92-93 1.96234567778232 1102 94-95 1.8359461595680813 1102 96-97 1.8355171700520678 1102 98-99 1.866731180994023 1102 100-101 1.8683743173987502 1103 1 0.1868199170465097 1103 2 0.251137894082607 1103 3 0.1629521994682115 1103 4 0.08586613501159235 1103 5 0.17723967178579159 1103 6 0.1713033740252783 1103 7 0.24780907175266975 1103 8 0.22946542895842015 1103 9 0.11741097590124383 1103 10-11 0.2754312006880255 1103 12-13 0.1460337715563398 1103 14-15 0.4627023397282102 1103 16-17 0.4248808447874026 1103 18-19 0.383912573733312 1103 20-21 0.34318110374177024 1103 22-23 0.3030935447401433 1103 24-25 0.41567847447166884 1103 26-27 0.15672293173075502 1103 28-29 0.20396804690934545 1103 30-31 0.201728702057828 1103 32-33 0.16989031423558032 1103 34-35 0.2228883272995006 1103 36-37 0.2833488000119502 1103 38-39 0.35807882532360935 1103 40-41 0.32004022282235667 1103 42-43 0.23118795708231943 1103 44-45 0.0850945346822698 1103 46-47 0.28860821790065216 1103 48-49 0.377332337102672 1103 50-51 0.015420006826424526 1103 52-53 -0.02624313403454437 1103 54-55 0.05367874656398186 1103 56-57 0.35126717507455396 1103 58-59 0.32588991596752237 1103 60-61 0.38044700524186936 1103 62-63 0.35606048331957396 1103 64-65 0.3593005132425162 1103 66-67 0.5256472684244109 1103 68-69 0.6063654593372299 1103 70-71 0.6344743877397363 1103 72-73 0.6879458204424154 1103 74-75 0.6886375179017641 1103 76-77 0.5109863845253635 1103 78-79 0.5824011166600798 1103 80-81 0.6806826873557981 1103 82-83 0.5722279648119368 1103 84-85 0.7121800696403433 1103 86-87 0.6281910252793566 1103 88-89 0.48125930941666795 1103 90-91 0.6568291028371682 1103 92-93 0.9475740310277914 1103 94-95 0.9465040484706542 1103 96-97 0.8749849616520216 1103 98-99 1.0542849437726929 1103 100-101 1.257154893824417 1104 1 0.18447694639985457 1104 2 0.26799009742256175 1104 3 0.3348642130045896 1104 4 0.24347431569282207 1104 5 0.28581320227942797 1104 6 0.25418309152693297 1104 7 0.2994897897692965 1104 8 0.3508885143700269 1104 9 0.3690728606948852 1104 10-11 0.3830704267602698 1104 12-13 0.30526514064336396 1104 14-15 0.458840940478602 1104 16-17 0.32549498441809277 1104 18-19 0.39118308061185303 1104 20-21 0.5077846496845311 1104 22-23 0.5130357208602092 1104 24-25 0.39059762403394416 1104 26-27 0.41369078283058514 1104 28-29 0.4214417466077194 1104 30-31 0.3684565766991881 1104 32-33 0.3500736438905463 1104 34-35 0.2915198288076297 1104 36-37 0.47315237538162336 1104 38-39 0.46284745623658097 1104 40-41 0.5728465201812938 1104 42-43 0.42058779523430445 1104 44-45 0.49962936878805664 1104 46-47 0.442315567271649 1104 48-49 0.5244242226319855 1104 50-51 0.42971795429938453 1104 52-53 0.4317770233994338 1104 54-55 0.4313148828105753 1104 56-57 0.5023864276310164 1104 58-59 0.6900944329983432 1104 60-61 0.6321636240898059 1104 62-63 0.5682549952006966 1104 64-65 0.5141920015085333 1104 66-67 0.5490896284626707 1104 68-69 0.7114933928324128 1104 70-71 0.7693847092287385 1104 72-73 0.6680087940317634 1104 74-75 0.5506382535745367 1104 76-77 0.7561204976718372 1104 78-79 0.711794480891669 1104 80-81 0.9693003582158006 1104 82-83 1.0388893346346393 1104 84-85 1.043443514060261 1104 86-87 0.9669397930274606 1104 88-89 0.8290185972854225 1104 90-91 1.0648444048308434 1104 92-93 1.2978596191981744 1104 94-95 1.2517343876158016 1104 96-97 1.0848423882686618 1104 98-99 1.4549229192012234 1104 100-101 1.4428840191094885 1105 1 0.11871266673679415 1105 2 0.12202185373100605 1105 3 0.14911230801637743 1105 4 0.11864449617022643 1105 5 0.19696889130086248 1105 6 0.18916886868635885 1105 7 0.2571048065175816 1105 8 0.238360361410777 1105 9 0.2924968115451563 1105 10-11 0.38151963204900596 1105 12-13 0.2701119380361874 1105 14-15 0.29186634185197846 1105 16-17 0.36882580378686924 1105 18-19 0.21670538831651953 1105 20-21 0.3781092495738747 1105 22-23 0.33307012429296634 1105 24-25 0.27627118286645214 1105 26-27 0.2541801908894996 1105 28-29 0.3548980805356834 1105 30-31 0.37247668152807023 1105 32-33 0.33311218905122075 1105 34-35 0.4979830709724453 1105 36-37 0.47461185233924397 1105 38-39 0.6118405502955895 1105 40-41 0.6110155045166508 1105 42-43 0.637185184384542 1105 44-45 0.6003755800483219 1105 46-47 0.674585918331303 1105 48-49 0.7018126037536092 1105 50-51 0.5655314456795892 1105 52-53 0.4877973945478402 1105 54-55 0.48831570013603454 1105 56-57 0.6868387047778128 1105 58-59 0.7793557291938811 1105 60-61 0.7784331417529913 1105 62-63 0.6615156187098208 1105 64-65 0.64023851100076 1105 66-67 0.7893021560416678 1105 68-69 0.8967452599389638 1105 70-71 0.9962098015796315 1105 72-73 0.9072630878602652 1105 74-75 0.8260590269572781 1105 76-77 0.7968607282219011 1105 78-79 0.9121992406645631 1105 80-81 0.9607228029831987 1105 82-83 1.0785282420817133 1105 84-85 1.0150485139799095 1105 86-87 0.9300469039885755 1105 88-89 1.0146861868870225 1105 90-91 0.9934133161579126 1105 92-93 1.044677387761908 1105 94-95 1.147043662652706 1105 96-97 1.3967768323381158 1105 98-99 1.489858302276616 1105 100-101 1.4964423694895679 1106 1 0.06105668277072951 1106 2 0.08184218002841703 1106 3 0.1400447104814262 1106 4 0.1987924532824792 1106 5 0.004617863408377332 1106 6 0.0242054705879724 1106 7 0.022014661604302432 1106 8 0.05956199165639475 1106 9 0.1276542243210841 1106 10-11 -0.25413666871512675 1106 12-13 -0.3976455707447286 1106 14-15 -0.23770088393951738 1106 16-17 -0.17395038412542618 1106 18-19 -0.2031646440115935 1106 20-21 -0.07647890833961668 1106 22-23 -0.22209785684811578 1106 24-25 -0.10212336130986444 1106 26-27 -0.06515168704254393 1106 28-29 0.09602261941976309 1106 30-31 0.2840980094407328 1106 32-33 0.1406526497740117 1106 34-35 -0.07558988936987276 1106 36-37 -0.013185904509732893 1106 38-39 0.08822512797709692 1106 40-41 0.006965156153725616 1106 42-43 0.18587880616811248 1106 44-45 -0.08211051003360126 1106 46-47 0.11859634278093978 1106 48-49 0.29524107842837566 1106 50-51 0.27337931317799047 1106 52-53 0.2376342251509982 1106 54-55 0.29277364438785725 1106 56-57 0.1264635565517267 1106 58-59 0.013305412556739782 1106 60-61 -0.06925428982391679 1106 62-63 0.1450923516619227 1106 64-65 0.08580648763586396 1106 66-67 0.36581452197148323 1106 68-69 0.27442557160573244 1106 70-71 0.017108071236858535 1106 72-73 0.0753422225463467 1106 74-75 0.01201995274330514 1106 76-77 0.14733939676881747 1106 78-79 0.16165036157337553 1106 80-81 -0.05735629721833391 1106 82-83 -0.08618100488146396 1106 84-85 0.06949599791815331 1106 86-87 -0.0327959356624028 1106 88-89 0.27242239341177665 1106 90-91 0.6272292914246762 1106 92-93 0.6519756634884182 1106 94-95 0.5341803851582902 1106 96-97 0.4783811869551613 1106 98-99 0.554790181496088 1106 100-101 0.440054957178031 1107 1 0.27273638051907056 1107 2 0.36482154460985683 1107 3 0.4398640712817574 1107 4 0.37121095277022675 1107 5 0.5546810093835646 1107 6 0.3957738132412132 1107 7 0.43502487972632053 1107 8 0.30343268567965964 1107 9 0.3507628869813857 1107 10-11 0.42042375903375984 1107 12-13 0.47132418607231585 1107 14-15 0.6445711766134821 1107 16-17 0.6583950155971152 1107 18-19 0.7111659383110336 1107 20-21 0.7377730160885818 1107 22-23 0.7504947290714981 1107 24-25 0.7136036145964368 1107 26-27 0.6621519193490997 1107 28-29 0.6540823633854842 1107 30-31 0.7061381513754128 1107 32-33 0.8256487613048336 1107 34-35 0.8940123020928752 1107 36-37 0.7443640316532338 1107 38-39 0.619694455964229 1107 40-41 0.7809743245988585 1107 42-43 0.8503600651288039 1107 44-45 0.5986546854153545 1107 46-47 0.7288464141703273 1107 48-49 0.6197659163800324 1107 50-51 0.4670209441581932 1107 52-53 0.5524691114450491 1107 54-55 0.650674344904651 1107 56-57 0.7365166129921548 1107 58-59 0.8012816636479485 1107 60-61 0.9271553240613386 1107 62-63 0.9425841224468385 1107 64-65 0.8430229237051634 1107 66-67 0.9098806513687769 1107 68-69 1.2074409208781276 1107 70-71 0.995360039596985 1107 72-73 0.9252518782656551 1107 74-75 0.9678191892656436 1107 76-77 0.9382742657122272 1107 78-79 1.176485223186571 1107 80-81 1.257880825358903 1107 82-83 1.316815633832487 1107 84-85 1.4559620766703283 1107 86-87 1.3561870352860055 1107 88-89 1.2038621784118853 1107 90-91 1.251246468808226 1107 92-93 1.3079431370792811 1107 94-95 1.4114313102585498 1107 96-97 1.375956366699679 1107 98-99 1.3707125063934207 1107 100-101 1.3971038472428283 1108 1 0.027779738026815437 1108 2 0.01663001176510548 1108 3 0.01890732978823806 1108 4 0.10994325118791437 1108 5 0.11931417725822513 1108 6 0.07259788150594915 1108 7 4.970352601958439E-4 1108 8 0.01614315047377346 1108 9 0.047430341012479005 1108 10-11 0.09077898374140148 1108 12-13 0.012457709702736963 1108 14-15 0.060590710620296306 1108 16-17 0.054595073123572035 1108 18-19 0.07956400173095801 1108 20-21 0.046245037297921954 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92-93 0.8512336945462557 2316 94-95 1.0179533421898483 2316 96-97 1.2565018270318724 2316 98-99 1.6345632707270106 2316 100-101 1.643070690834083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14118.0 3 3662.0 4 3649.0 5 3992.0 6 4501.0 7 5391.0 8 6403.0 9 8091.0 10 9661.0 11 11527.0 12 14048.0 13 16634.0 14 18743.0 15 21370.0 16 24254.0 17 28566.0 18 26330.0 19 22941.0 20 23664.0 21 25196.0 22 27506.0 23 30605.0 24 34278.0 25 37739.0 26 43753.0 27 50358.0 28 58605.0 29 68704.0 30 81938.0 31 99207.0 32 122227.0 33 152966.0 34 197516.0 35 261949.0 36 350946.0 37 419415.0 38 271081.0 39 28258.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.061666371621996 18.269700336350926 13.880122331626144 23.788510960400934 2 15.47648919318193 19.24672981162525 40.70695252992954 24.56982846526328 3 19.8259380121517 23.0967308468134 28.95309615125029 28.124234989784615 4 12.814560924621308 15.427261349361974 36.298509784899245 35.45966794111747 5 13.903653788216811 37.09329153652835 34.08289191178434 14.920162763470508 6 33.63834557154926 35.10346370281961 16.76667973753095 14.49151098810018 7 29.764577002581515 31.988911856882936 19.828610076492946 18.4179010640426 8 26.71926785606778 35.1948367666234 18.995374946368734 19.090520430940078 9 26.29403546693353 16.736964373772427 19.977264395341905 36.991735763952136 10-11 25.44307162026597 26.22374271312357 26.739185317926456 21.594000348684002 12-13 26.14886689462843 23.385794261681482 27.547217407656717 22.918121436033367 14-15 22.841619803312046 25.00543497417792 25.627127427994928 26.525817794515106 16-17 22.529528385941592 28.31189321336239 26.382291941109536 22.776286459586483 18-19 22.5189123611055 26.896608615669273 28.48481257577709 22.099666447448136 20-21 23.003582383193468 25.953914771815946 28.797171886959294 22.245330958031293 22-23 22.651100400587307 25.886128195177672 28.82237771723341 22.640393687001612 24-25 22.56947639445732 26.109693403707503 28.87615651108717 22.444673690748008 26-27 22.27260993603635 26.35811071474498 28.8360330153408 22.533246333877873 28-29 22.247362365022045 26.81561105694601 28.690826481506498 22.246200096525445 30-31 22.392093852821574 26.276747541618295 28.64642725608001 22.684731349480124 32-33 22.129911207652146 26.310754163332188 28.786898365153768 22.772436263861895 34-35 22.732832723512022 26.38503490118761 28.58580265872438 22.29632971657599 36-37 22.69857099179056 26.129733714706983 28.562124186461617 22.60957110704084 38-39 22.354914058242567 26.520430726880655 28.56875102499824 22.555904189878532 40-41 22.729507277161048 26.390991721637665 28.506075871877474 22.373425129323817 42-43 22.3555295036975 26.351064958597803 28.38809462260059 22.90531091510411 44-45 22.457835621934223 26.519537833495928 28.404235478523994 22.618391066045856 46-47 22.860545778642404 26.83018270536995 27.82725448924241 22.48201702674524 48-49 22.75955208094568 26.849744844055067 27.749264103313052 22.6414389716862 50-51 22.75345464078747 27.01796977170258 27.75477690016675 22.473798687343198 52-53 22.948056751844746 26.872323594748487 27.657615269204612 22.52200438420216 54-55 22.66856158746609 26.950898436309323 27.764596732404666 22.615943243819917 56-57 22.73751043055304 27.110562052767122 27.554411726332003 22.597515790347835 58-59 22.889234237005525 26.804895223840624 27.59255686133037 22.71331367782348 60-61 22.587545596119085 27.013585372370763 27.886398839321302 22.512470192188843 62-63 22.52193037172449 27.03828132395316 28.02166020013458 22.418128104187765 64-65 22.422264639815086 27.0719112790885 27.855826233296167 22.649997847800243 66-67 22.406647539322538 27.025436421896593 27.687859113854422 22.880056924926446 68-69 22.629416660793975 26.80411939150028 27.91921970024513 22.647244247460616 70-71 22.633978042088092 26.86968141942842 27.697379122346355 22.798961416137136 72-73 22.577292392609642 26.980270857875325 27.669957555251024 22.772479194264005 74-75 22.45455359370922 27.338858844214503 27.522644625580284 22.683942936495992 76-77 22.688246329531577 27.028579167430962 27.521041657023705 22.762132846013756 78-79 22.68115015828382 27.115840058578062 27.507363688192974 22.695646094945147 80-81 22.767774916673158 27.194083018085912 27.35948910774995 22.67865295749098 82-83 22.78120081467326 27.237299771384937 27.40307187806181 22.578427535879996 84-85 22.65541610956934 27.36849281599154 27.298299028960553 22.677792045478565 86-87 22.751552390489007 27.302666535134513 27.30973194483115 22.636049129545334 88-89 22.76009124986335 27.32027993550498 27.040504411082996 22.879124403548673 90-91 22.756523070734406 27.146129888493736 27.236791581718943 22.860555459052915 92-93 22.796733901578996 26.986298186893325 27.44356491686769 22.773402994659982 94-95 22.73173806884625 26.838591840077402 27.50654218423248 22.923127906843867 96-97 22.79665952926683 26.71928991188657 27.46932638614565 23.01472417270095 98-99 22.670943158444935 26.897801167514256 27.235433977689755 23.19582169635105 100-101 23.0666930248361 26.174100279884144 27.71753869546868 23.041667999811075 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3093.0 1 1827.0 2 539.0 3 503.5 4 433.5 5 390.0 6 603.0 7 1109.5 8 1758.5 9 2114.5 10 1976.5 11 1886.5 12 1994.0 13 2177.5 14 2362.0 15 2522.5 16 2747.5 17 2882.5 18 3043.5 19 3386.5 20 3876.5 21 4648.5 22 5649.5 23 7012.0 24 8848.0 25 11353.5 26 14854.0 27 18334.0 28 20869.0 29 24071.5 30 28694.5 31 33918.0 32 40711.0 33 45343.0 34 48374.5 35 52938.5 36 58928.5 37 65164.0 38 69027.0 39 72348.0 40 75332.0 41 78609.0 42 81082.5 43 83672.0 44 88057.5 45 92708.5 46 95373.0 47 98978.0 48 103366.5 49 102009.0 50 97308.0 51 93211.0 52 91044.5 53 90607.5 54 90998.5 55 86259.0 56 78448.0 57 72621.5 58 67799.0 59 60721.0 60 50000.5 61 41219.5 62 34485.5 63 26719.0 64 19519.0 65 14755.5 66 11118.0 67 8517.0 68 6475.0 69 4812.5 70 3623.5 71 2567.0 72 1855.0 73 1416.5 74 1052.5 75 781.0 76 586.0 77 401.0 78 258.0 79 173.5 80 133.0 81 101.0 82 60.0 83 38.5 84 31.0 85 29.0 86 23.0 87 12.5 88 10.5 89 8.5 90 5.5 91 6.5 92 6.0 93 4.5 94 4.5 95 4.0 96 3.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33162305930542824 2 0.28393869783667386 3 0.2767898461970201 4 0.28469921396855186 5 0.2883496914015666 6 0.2143134459632374 7 0.2041225297960714 8 0.20499712334773115 9 0.21731748468415574 10-11 0.21448456209290997 12-13 0.20999751691482943 14-15 0.2123931427302453 16-17 0.19611809750805484 18-19 0.20225926527297017 20-21 0.1902811361958907 22-23 0.20045303945975976 24-25 0.1996544975212878 26-27 0.19984462655425733 28-29 0.2134958911214685 30-31 0.2112903943390221 32-33 0.21648091693908986 34-35 0.21016863304450195 36-37 0.19280985233438525 38-39 0.18208657487490462 40-41 0.17858820066826553 42-43 0.19444496201792308 44-45 0.1960040200882731 46-47 0.19611809750805484 48-49 0.2134578653148746 50-51 0.20902785884668487 52-53 0.23022724602278583 54-55 0.19898904590589447 56-57 0.1784360974418899 58-59 0.18041343938477286 60-61 0.16854938772747513 62-63 0.17527995549459596 64-65 0.1738539877473246 66-67 0.17419622000666973 68-69 0.17674394904846125 70-71 0.17796077485946613 72-73 0.1755081103341594 74-75 0.19005298135632728 76-77 0.1833414264925034 78-79 0.1870679555387059 80-81 0.18018528454520943 82-83 0.15972740059768964 84-85 0.15987950382406524 86-87 0.16449963932522446 88-89 0.17012745870112211 90-91 0.17678197485505512 92-93 0.17187664580444165 94-95 0.17199072322422335 96-97 0.17697210388802465 98-99 0.1863264523101248 100-101 0.16811209095164525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2629793.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.90635027231765 #Duplication Level Percentage of deduplicated Percentage of total 1 77.51056094523625 43.33332570008203 2 12.500069460625522 13.976665233880622 3 4.247109515071189 7.123211765833892 4 1.8996226424118963 4.248038753276203 5 1.0155039107100476 2.838655866753216 6 0.6325498723514476 2.121813283703391 7 0.39839516547648757 1.5590973767548528 8 0.2827038780967155 1.2643953625774051 9 0.21640167852863143 1.0888405235405236 >10 1.1649068807399428 12.095516927482239 >50 0.08707478481770359 3.3498740827101434 >100 0.04053303646808376 4.011894819064795 >500 0.0031363963498808973 1.2048833575682574 >1k 0.0012954680575525345 1.2799002972856743 >5k 1.3636505868974049E-4 0.5038866494868312 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7387 0.2808966333091616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5832 0.22176650405564238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3664 0.13932655536006067 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3603 0.13700698115783258 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2687 0.10217534231781741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.802580659390302E-5 0.0 0.0 0.0 0.0 6 3.802580659390302E-5 0.0 0.0 0.0 0.0 7 7.605161318780604E-5 0.0 0.0 0.0 0.0 8 7.605161318780604E-5 0.0 0.0 0.0 0.0 9 7.605161318780604E-5 0.0 0.0 7.605161318780604E-5 0.0 10-11 7.605161318780604E-5 0.0 0.0 7.605161318780604E-5 0.0 12-13 1.1407741978170905E-4 0.0 0.0 7.605161318780604E-5 3.802580659390302E-5 14-15 1.5210322637561208E-4 0.0 0.0 7.605161318780604E-5 3.802580659390302E-5 16-17 1.5210322637561208E-4 0.0 0.0 7.605161318780604E-5 3.802580659390302E-5 18-19 1.711161296725636E-4 0.0 0.0 1.5210322637561208E-4 3.802580659390302E-5 20-21 1.901290329695151E-4 3.802580659390302E-5 0.0 1.5210322637561208E-4 3.802580659390302E-5 22-23 1.901290329695151E-4 3.802580659390302E-5 0.0 1.711161296725636E-4 3.802580659390302E-5 24-25 1.901290329695151E-4 3.802580659390302E-5 0.0 3.2321935604817567E-4 3.802580659390302E-5 26-27 2.091419362664666E-4 3.802580659390302E-5 0.0 3.992709692359817E-4 3.802580659390302E-5 28-29 2.8519354945427265E-4 3.802580659390302E-5 0.0 7.034774219872059E-4 3.802580659390302E-5 30-31 3.0420645275122416E-4 5.7038709890854533E-5 0.0 0.0012928774241927027 3.802580659390302E-5 32-33 3.2321935604817567E-4 9.506451648475755E-5 0.0 0.00264279355827626 3.802580659390302E-5 34-35 4.753225824237877E-4 1.1407741978170905E-4 0.0 0.00403073549895372 3.802580659390302E-5 36-37 5.323612923146423E-4 1.3309032307866057E-4 0.0 0.006103141958321434 3.802580659390302E-5 38-39 5.323612923146423E-4 1.5210322637561208E-4 0.0 0.009468425841881851 3.802580659390302E-5 40-41 5.703870989085453E-4 1.5210322637561208E-4 0.0 0.013328045211163007 3.802580659390302E-5 42-43 6.464387120963513E-4 1.5210322637561208E-4 0.0 0.016807406514505133 3.802580659390302E-5 44-45 7.224903252841574E-4 1.5210322637561208E-4 0.0 0.019811445235423473 3.802580659390302E-5 46-47 8.175548417689148E-4 1.5210322637561208E-4 0.0 0.024184412993722322 3.802580659390302E-5 48-49 8.745935516597694E-4 1.5210322637561208E-4 0.0 0.028025019459706525 3.802580659390302E-5 50-51 8.745935516597694E-4 1.5210322637561208E-4 0.0 0.03177056140920597 3.802580659390302E-5 52-53 8.745935516597694E-4 1.5210322637561208E-4 0.0 0.04171430983351161 3.802580659390302E-5 54-55 8.745935516597694E-4 1.5210322637561208E-4 0.0 0.05778021311943564 3.802580659390302E-5 56-57 9.126193582536724E-4 1.5210322637561208E-4 0.0 0.08078582610874696 3.802580659390302E-5 58-59 9.126193582536724E-4 1.5210322637561208E-4 0.0 0.11168179396629316 3.802580659390302E-5 60-61 9.126193582536724E-4 1.5210322637561208E-4 0.0 0.15014489733602607 3.802580659390302E-5 62-63 9.126193582536724E-4 1.5210322637561208E-4 0.0 0.17605948452977097 3.802580659390302E-5 64-65 9.31632261550624E-4 1.5210322637561208E-4 0.0 0.20775399432578914 3.802580659390302E-5 66-67 9.506451648475754E-4 1.5210322637561208E-4 0.0 0.26321463324299665 3.802580659390302E-5 68-69 0.0010266967780353815 1.5210322637561208E-4 0.0 0.3359199754505393 3.802580659390302E-5 70-71 0.0010457096813323331 1.5210322637561208E-4 0.0 0.4092717563701782 3.802580659390302E-5 72-73 0.0010647225846292846 1.5210322637561208E-4 0.0 0.4872626856942733 3.802580659390302E-5 74-75 0.0010647225846292846 1.5210322637561208E-4 0.0 0.5666795827656397 3.802580659390302E-5 76-77 0.0010647225846292846 1.5210322637561208E-4 0.0 0.6493857121073787 3.802580659390302E-5 78-79 0.0010647225846292846 1.5210322637561208E-4 0.0 0.7311792220908642 3.802580659390302E-5 80-81 0.001273864520895751 1.5210322637561208E-4 0.0 0.8157486159557045 3.802580659390302E-5 82-83 0.0013309032307866056 1.5210322637561208E-4 0.0 0.9239890744252495 3.802580659390302E-5 84-85 0.001425967747271363 1.5210322637561208E-4 0.0 1.0698750814227584 3.802580659390302E-5 86-87 0.0015210322637561207 1.5210322637561208E-4 0.0 1.2420179078733573 3.802580659390302E-5 88-89 0.0015210322637561207 1.5210322637561208E-4 0.0 1.448840269937596 3.802580659390302E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7050 0.0 32.91748 1 GTATCAA 10560 0.0 31.478142 1 ATCAACG 12710 0.0 25.729517 3 GTACATG 9040 0.0 25.671263 1 TCAACGC 12785 0.0 25.619179 4 TATCAAC 12925 0.0 25.450079 2 CAACGCA 12930 0.0 25.220139 5 TACATGG 9010 0.0 25.112833 2 AACGCAG 13310 0.0 24.556543 6 ACATGGG 9385 0.0 23.145718 3 GTACACG 425 0.0 22.378807 1 ACGCAGA 15095 0.0 21.432404 7 CGCAGAG 15330 0.0 21.103855 8 GAGTACT 8410 0.0 20.899193 12-13 TAAGGTG 1125 0.0 20.70453 5 CATGGGG 4815 0.0 20.5359 4 GTACTTT 8645 0.0 20.056337 14-15 AGAGTAC 12475 0.0 19.91464 10-11 GAGTACA 6955 0.0 19.828814 1 GTATCGA 120 0.0047333264 19.81457 1 >>END_MODULE