FastQCFastQC Report
Thu 26 May 2016
SRR937906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629793
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.32070965281297803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72060.27401396231566516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46900.17834103292540515No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31600.12016154883673354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113500.031.9448241
GGTATCA75900.031.1161921
ATCAACG136750.026.2996223
TCAACGC137050.026.2073884
CAACGCA139650.025.719465
TATCAAC140400.025.6497462
AACGCAG143500.025.0553866
GTACATG94100.022.3710291
ACGCAGA164150.021.7595837
GAGTACT87900.021.74956712-13
TACATGG94000.021.632012
CGCAGAG166400.021.408278
ACATGGG95050.020.893213
GTACTTT93250.020.42455514-15
GGACCGT2801.5807018E-920.355436
AGAGTAC136300.020.15956310-11
AGTACTT93950.019.91925412-13
GCAGAGT180750.019.39339
GAGTACA74650.018.524011
GGACCGA5650.017.6533816
Produced by FastQC (version 0.11.5)