##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937906_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2629793 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.050328676059294 31.0 31.0 34.0 30.0 34.0 2 32.26156317246262 34.0 31.0 34.0 30.0 34.0 3 32.35730340753055 34.0 31.0 34.0 30.0 34.0 4 36.01388284172937 37.0 35.0 37.0 35.0 37.0 5 35.90541727048479 37.0 35.0 37.0 35.0 37.0 6 35.933922936139844 37.0 35.0 37.0 35.0 37.0 7 35.87068411848385 37.0 35.0 37.0 35.0 37.0 8 35.84075704817832 37.0 35.0 37.0 35.0 37.0 9 37.4987814630277 39.0 37.0 39.0 35.0 39.0 10-11 37.41531804974764 39.0 37.0 39.0 34.0 39.0 12-13 37.30766547024804 39.0 37.0 39.0 34.0 39.0 14-15 38.49699501063392 40.0 38.0 41.0 34.0 41.0 16-17 38.44803659451523 40.0 38.0 41.0 34.0 41.0 18-19 38.40616143551983 40.0 38.0 41.0 34.0 41.0 20-21 38.272661764633185 40.0 38.0 41.0 34.0 41.0 22-23 38.10610930213899 40.0 37.5 41.0 33.0 41.0 24-25 38.186282342374476 40.0 38.0 41.0 33.0 41.0 26-27 38.04583421584893 40.0 37.0 41.0 33.0 41.0 28-29 37.89774879619803 40.0 37.0 41.0 33.0 41.0 30-31 37.965266467740996 40.0 37.0 41.0 33.0 41.0 32-33 37.906240909455605 40.0 37.0 41.0 33.0 41.0 34-35 37.7750746921906 40.0 37.0 41.0 33.0 41.0 36-37 37.60123724566915 40.0 37.0 41.0 33.0 41.0 38-39 37.444819991535454 40.0 37.0 41.0 32.0 41.0 40-41 37.257149326962235 40.0 36.0 41.0 31.5 41.0 42-43 37.04086405279807 39.0 36.0 41.0 31.0 41.0 44-45 36.77057186630279 39.0 36.0 41.0 31.0 41.0 46-47 36.466401157809756 39.0 35.5 41.0 30.0 41.0 48-49 36.16223063944577 39.0 35.0 40.0 30.0 41.0 50-51 35.90438011660994 39.0 35.0 40.0 29.0 41.0 52-53 35.639412683812 38.5 35.0 40.0 28.0 41.0 54-55 35.39598477903014 38.0 34.5 40.0 28.0 41.0 56-57 35.09257876950772 38.0 34.0 40.0 27.0 41.0 58-59 34.7959274741396 38.0 34.0 40.0 26.5 41.0 60-61 34.39940767201068 37.0 33.5 40.0 26.0 41.0 62-63 34.016322387351394 37.0 33.0 39.5 26.0 41.0 64-65 33.59767555849453 36.0 33.0 39.0 25.0 40.5 66-67 33.12466950820844 36.0 32.0 39.0 23.0 40.0 68-69 32.658850525497634 35.0 31.5 38.5 22.0 40.0 70-71 32.21310194376515 35.0 31.0 38.0 21.5 40.0 72-73 31.68288853913597 35.0 31.0 37.0 20.0 39.0 74-75 31.16052917472972 34.0 30.0 36.5 18.5 39.0 76-77 29.963835176380805 33.5 29.0 35.0 18.0 37.5 78-79 30.58731865968158 34.0 30.0 36.0 18.0 37.5 80-81 30.387130469964745 34.0 30.0 35.0 17.5 37.0 82-83 30.003613022013518 34.0 30.0 35.0 13.5 37.0 84-85 29.59088110737233 34.0 29.0 35.0 7.5 36.0 86-87 29.138110870323253 34.0 29.0 35.0 4.5 36.0 88-89 28.70142060610854 33.5 29.0 35.0 2.0 35.5 90-91 28.10195384199441 33.0 27.5 35.0 2.0 35.0 92-93 27.556721004276763 33.0 26.5 35.0 2.0 35.0 94-95 26.997751724185136 32.5 25.5 34.5 2.0 35.0 96-97 26.340992427921133 32.0 25.0 34.0 2.0 35.0 98-99 25.334249691895902 32.0 22.0 34.0 2.0 35.0 100-101 22.607308826207994 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19756394751182427 1101 2 0.17947224624870728 1101 3 0.18187309234066618 1101 4 0.1875414719108619 1101 5 0.15406244935038416 1101 6 0.10711880091277948 1101 7 0.10787482258901804 1101 8 0.10226692034388662 1101 9 0.2955639963472123 1101 10-11 0.2347319982034577 1101 12-13 0.19541794468058526 1101 14-15 0.20392476022946227 1101 16-17 0.19255261987456862 1101 18-19 0.274865335458081 1101 20-21 0.3099524344143916 1101 22-23 0.2235629343785135 1101 24-25 0.3048471380091726 1101 26-27 0.20400069377229357 1101 28-29 0.2720341936718569 1101 30-31 0.387345405760378 1101 32-33 0.28891398704493554 1101 34-35 0.3038755497707868 1101 36-37 0.3301219767337713 1101 38-39 0.22335801145627698 1101 40-41 0.20633701061378673 1101 42-43 0.3171147733575097 1101 44-45 0.2855635348212857 1101 46-47 0.35147009457922707 1101 48-49 0.3596907119191215 1101 50-51 0.3025413358695843 1101 52-53 0.22779334369291604 1101 54-55 0.3248769952778403 1101 56-57 0.289439741953629 1101 58-59 0.2479064857630462 1101 60-61 0.16455744456301602 1101 62-63 0.17305852050939308 1101 64-65 0.3228475140782834 1101 66-67 0.285475368526555 1101 68-69 0.27274554905259407 1101 70-71 0.30061457326092267 1101 72-73 0.3570333555239813 1101 74-75 0.144697878948584 1101 76-77 0.11263788890053661 1101 78-79 0.3576176078670521 1101 80-81 0.25554967651719096 1101 82-83 0.2805515570584802 1101 84-85 0.09762480755850689 1101 86-87 -0.04123141587525225 1101 88-89 0.18487923923365912 1101 90-91 0.24369742867571276 1101 92-93 0.43728906506360943 1101 94-95 0.4354761498662931 1101 96-97 0.6575951074083513 1101 98-99 0.6966054653376013 1101 100-101 0.9262574701817172 1102 1 0.2644587248832977 1102 2 0.2661958875465871 1102 3 0.2771695801931031 1102 4 0.16684965657159978 1102 5 0.12805319726921027 1102 6 0.1386386511240758 1102 7 0.1502509120813471 1102 8 0.20602317835885486 1102 9 0.21449883225965038 1102 10-11 0.24367751815302796 1102 12-13 0.265452467912219 1102 14-15 0.3259577987189104 1102 16-17 0.2487970630884817 1102 18-19 0.24812600319359035 1102 20-21 0.22764220092328458 1102 22-23 0.36265524145776595 1102 24-25 0.26925718457491854 1102 26-27 0.21714427180949514 1102 28-29 0.2914392798842016 1102 30-31 0.26019637211070545 1102 32-33 0.1982023975686431 1102 34-35 0.25448956492975583 1102 36-37 0.3635411610105095 1102 38-39 0.29106547687869977 1102 40-41 0.43350450650732597 1102 42-43 0.3833582617616216 1102 44-45 0.25518162901055774 1102 46-47 0.3095974149740641 1102 48-49 0.3453069161542359 1102 50-51 0.46061152292950425 1102 52-53 0.3657739054212428 1102 54-55 0.3097006297445617 1102 56-57 0.22982778287020977 1102 58-59 0.3080215959775927 1102 60-61 0.17935971354302893 1102 62-63 0.2819332965560406 1102 64-65 0.2930069259154422 1102 66-67 0.34949581905187443 1102 68-69 0.39206430131023495 1102 70-71 0.23370330019152874 1102 72-73 0.23479641727041667 1102 74-75 0.2291706014037409 1102 76-77 0.08752380335992527 1102 78-79 0.3364374551802847 1102 80-81 0.31769226972888376 1102 82-83 0.3244205395572557 1102 84-85 0.40054145395594887 1102 86-87 0.4894363506771171 1102 88-89 0.6357428245988324 1102 90-91 0.7952808992360012 1102 92-93 0.7157985474376645 1102 94-95 0.7841544498597735 1102 96-97 0.9823571682864021 1102 98-99 1.0026148933728898 1102 100-101 1.1795740757992093 1103 1 0.26717593143582263 1103 2 0.27071959736306894 1103 3 0.1623276045803408 1103 4 0.0821267842966833 1103 5 0.118911554209717 1103 6 0.15590506324380016 1103 7 0.1399566640212413 1103 8 0.17479888965334567 1103 9 0.2170429879438487 1103 10-11 0.16293660178402547 1103 12-13 0.15094735644529322 1103 14-15 0.18713411353600407 1103 16-17 0.2518555062465069 1103 18-19 0.15656792953920018 1103 20-21 0.22336858348270994 1103 22-23 0.24546574767774842 1103 24-25 0.1936713981480196 1103 26-27 0.13198754068497465 1103 28-29 0.10951903108508532 1103 30-31 0.19565523403952056 1103 32-33 0.17890741050127446 1103 34-35 0.14384632068784953 1103 36-37 0.09735179039836339 1103 38-39 0.14986440531816925 1103 40-41 0.26577288931309084 1103 42-43 0.2828334434342352 1103 44-45 0.3483373747920524 1103 46-47 0.4358427653866812 1103 48-49 0.2704791664313646 1103 50-51 0.39265167565767456 1103 52-53 0.22699246682042684 1103 54-55 0.2755879927202969 1103 56-57 0.15191252237014652 1103 58-59 0.09401974845943073 1103 60-61 0.08131155490280406 1103 62-63 0.20626714308886562 1103 64-65 0.3144587672752195 1103 66-67 0.24233908792735548 1103 68-69 0.1228982710109392 1103 70-71 0.14851811728723163 1103 72-73 0.2717311998791132 1103 74-75 0.10656707880243488 1103 76-77 0.16228202481577014 1103 78-79 0.24069543030037366 1103 80-81 0.1425310429768203 1103 82-83 0.10298341675888167 1103 84-85 0.15468728472328408 1103 86-87 0.3074686206611048 1103 88-89 0.3299884829311388 1103 90-91 0.14513549955623617 1103 92-93 0.21839246294449666 1103 94-95 0.44088791457217624 1103 96-97 0.5527182349202242 1103 98-99 0.4671103977453477 1103 100-101 0.7060996331338565 1104 1 0.22384539365902612 1104 2 0.2163816292912628 1104 3 0.06834864482164704 1104 4 0.05211722055064172 1104 5 0.06636333085946688 1104 6 0.10255466230213983 1104 7 0.14804082498644533 1104 8 0.08062688935908113 1104 9 0.1743421860605281 1104 10-11 0.2614105221842209 1104 12-13 0.2008130225969893 1104 14-15 0.15949371038733062 1104 16-17 0.16963171458903759 1104 18-19 0.21314999383699984 1104 20-21 0.1896835985640024 1104 22-23 0.26673610742173537 1104 24-25 0.1499380059608555 1104 26-27 0.1482238387795789 1104 28-29 0.01067080037810797 1104 30-31 0.33020860709918054 1104 32-33 0.28425810571204124 1104 34-35 0.15524924866548417 1104 36-37 0.18883181688258333 1104 38-39 0.16123996626865278 1104 40-41 0.22159103100366195 1104 42-43 0.3474028541603431 1104 44-45 0.30586198304630585 1104 46-47 0.2858102486501224 1104 48-49 0.16102635983973101 1104 50-51 0.2026680075932319 1104 52-53 0.23387012443831878 1104 54-55 0.12910494997770883 1104 56-57 0.2192021567407778 1104 58-59 0.194993190672335 1104 60-61 0.13239784931905518 1104 62-63 0.18246901169771235 1104 64-65 0.20004252362038955 1104 66-67 0.09047386317931938 1104 68-69 0.08117444256982509 1104 70-71 0.16747875879388374 1104 72-73 0.15482602809952084 1104 74-75 0.045954704786396405 1104 76-77 0.12986357414262883 1104 78-79 0.15194563261038851 1104 80-81 0.39502482654188853 1104 82-83 0.5463922301187019 1104 84-85 0.3627635004225418 1104 86-87 0.3404791407817527 1104 88-89 0.36284392323129566 1104 90-91 0.3215274660095595 1104 92-93 0.4591178362984323 1104 94-95 0.5404415083336715 1104 96-97 0.5848503617502132 1104 98-99 0.6724507935372976 1104 100-101 0.9779828082975399 1105 1 0.11387264549399134 1105 2 0.17197250279637188 1105 3 0.1351113931473904 1105 4 0.10346534839320043 1105 5 0.03235524484743735 1105 6 0.04360230824409683 1105 7 0.06393849400177487 1105 8 0.08176838997781033 1105 9 0.14063015094308184 1105 10-11 0.13324614104423915 1105 12-13 0.16572335786113257 1105 14-15 0.17341740065771205 1105 16-17 0.1899092493885064 1105 18-19 0.1560998155636213 1105 20-21 0.12850190895023417 1105 22-23 0.11192324281078214 1105 24-25 0.10237879004230166 1105 26-27 -0.05591140333012845 1105 28-29 0.009576077866995547 1105 30-31 0.08748096703462949 1105 32-33 0.10791743940812637 1105 34-35 0.06427874355375707 1105 36-37 0.011198525104276769 1105 38-39 -0.0503968772020329 1105 40-41 -0.010334596970906773 1105 42-43 0.024031248873448874 1105 44-45 -0.04015303875286946 1105 46-47 -0.018568124826288113 1105 48-49 0.11783070911320692 1105 50-51 0.1867988646140759 1105 52-53 0.22883106041372514 1105 54-55 0.22177358649057766 1105 56-57 0.19484367687430648 1105 58-59 0.18190978444550154 1105 60-61 0.17506189411583506 1105 62-63 0.13394008273844094 1105 64-65 0.2601304090662637 1105 66-67 0.1271524912663935 1105 68-69 -0.05504633402711079 1105 70-71 0.1626912634142954 1105 72-73 0.2107970956964671 1105 74-75 0.322276889433077 1105 76-77 0.23204056413498364 1105 78-79 0.3068858025497967 1105 80-81 0.20033801601675805 1105 82-83 0.3683197980933173 1105 84-85 0.23550759030997526 1105 86-87 0.33386729920514213 1105 88-89 0.03981256410629186 1105 90-91 0.10586477806488048 1105 92-93 0.1873993061985857 1105 94-95 0.28236180991758175 1105 96-97 0.5965270957543964 1105 98-99 0.6609452157501856 1105 100-101 0.8485045649962828 1106 1 -0.005558616911820025 1106 2 0.03297298236152102 1106 3 0.03127967683293065 1106 4 -0.015583940144551889 1106 5 -0.04619044511254344 1106 6 0.008389674208395093 1106 7 0.05825360094755894 1106 8 0.025607311264295163 1106 9 0.0708648022146221 1106 10-11 -0.024536826530628275 1106 12-13 -0.08806132152680135 1106 14-15 0.059222042462089064 1106 16-17 0.1755431581846878 1106 18-19 0.058784127438215705 1106 20-21 0.004421726853415464 1106 22-23 0.007838237474871335 1106 24-25 0.12191756378089025 1106 26-27 0.02206017634274815 1106 28-29 0.09033343445608466 1106 30-31 0.06440310137999461 1106 32-33 0.10793002626895998 1106 34-35 0.09717091220436203 1106 36-37 -0.01108355207949785 1106 38-39 0.019369333640185005 1106 40-41 0.048217985301185706 1106 42-43 -0.05719113187553404 1106 44-45 0.051289240777052214 1106 46-47 0.24513494342507158 1106 48-49 0.2542943506725166 1106 50-51 0.42357961460246685 1106 52-53 0.30046156351571796 1106 54-55 0.3748206183593581 1106 56-57 0.32484502866866904 1106 58-59 0.26207555857537557 1106 60-61 0.2851109449400795 1106 62-63 0.2511985885826604 1106 64-65 0.21265598855613632 1106 66-67 0.08977261819712368 1106 68-69 0.22454882425007838 1106 70-71 0.27732267874760197 1106 72-73 0.23934995844290086 1106 74-75 0.08506450782428487 1106 76-77 -0.02147151488337329 1106 78-79 -0.02409374949129628 1106 80-81 -0.09847601432137054 1106 82-83 0.0037998828057617118 1106 84-85 0.0243744946594866 1106 86-87 0.07413035655019229 1106 88-89 -0.25952068860982536 1106 90-91 -0.045858621817504996 1106 92-93 0.1155972424591809 1106 94-95 0.2054327556724651 1106 96-97 0.28425937793733524 1106 98-99 0.3340340934711854 1106 100-101 0.5416025282467842 1107 1 0.14145390097254307 1107 2 0.17378023892866423 1107 3 0.18855807419623005 1107 4 0.08204255110373992 1107 5 0.1021832220902752 1107 6 0.1303128658974586 1107 7 0.14002202391230867 1107 8 0.11705988588501981 1107 9 0.15935604408876003 1107 10-11 0.17636856606407747 1107 12-13 0.2316440852840813 1107 14-15 0.24589632893231084 1107 16-17 0.20358437669838736 1107 18-19 0.1834215347831858 1107 20-21 0.20903659070270209 1107 22-23 0.22157119750200138 1107 24-25 0.27048972678413463 1107 26-27 0.1668350659391038 1107 28-29 0.12821873658558047 1107 30-31 0.16574776388529955 1107 32-33 0.165273223224915 1107 34-35 0.26364903768332226 1107 36-37 0.034370505367171234 1107 38-39 0.19629190460567258 1107 40-41 0.189372686646756 1107 42-43 0.20888480668196507 1107 44-45 0.27085379393135867 1107 46-47 0.2131718131715985 1107 48-49 0.15500603555324943 1107 50-51 0.24506802829708363 1107 52-53 0.13334839956053912 1107 54-55 0.41300425035986876 1107 56-57 0.3963409083228342 1107 58-59 0.21753999925900303 1107 60-61 0.3156349343638354 1107 62-63 0.3905521426138634 1107 64-65 0.4620115713538766 1107 66-67 0.45614218492219294 1107 68-69 0.4600382691109459 1107 70-71 0.38986825503699407 1107 72-73 0.3648492213490755 1107 74-75 0.2821913227620314 1107 76-77 0.3962257215762719 1107 78-79 0.49559336880380656 1107 80-81 0.4516904210445212 1107 82-83 0.4474106627134731 1107 84-85 0.6948861510585083 1107 86-87 0.6237084002614708 1107 88-89 0.7007922449248696 1107 90-91 0.7739422382116707 1107 92-93 0.6161264000546041 1107 94-95 0.9673658271089884 1107 96-97 0.955462653679529 1107 98-99 1.2951250910541923 1107 100-101 1.3504368192385456 1108 1 0.07189998577289458 1108 2 0.04862822272706069 1108 3 0.04374289297705758 1108 4 -0.01784768244552737 1108 5 -0.0848152686210284 1108 6 -0.1159419667219197 1108 7 0.027051007974307595 1108 8 -0.08107132106775339 1108 9 -0.03256199247586977 1108 10-11 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0.48901011358962165 2316 78-79 0.615325933921266 2316 80-81 0.5594215350328824 2316 82-83 0.6294411087488605 2316 84-85 0.6679763779853936 2316 86-87 0.7541142136340895 2316 88-89 0.7579031234576163 2316 90-91 0.9761932411097796 2316 92-93 0.9063187784441276 2316 94-95 1.051761830369184 2316 96-97 1.2212444898421637 2316 98-99 1.3312965364682618 2316 100-101 1.6395895139059462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 35.0 5 58.0 6 132.0 7 313.0 8 801.0 9 1437.0 10 2504.0 11 3862.0 12 5928.0 13 8104.0 14 10423.0 15 13771.0 16 17592.0 17 22859.0 18 21126.0 19 16007.0 20 14978.0 21 15177.0 22 16409.0 23 18706.0 24 21675.0 25 25994.0 26 31618.0 27 39251.0 28 48490.0 29 59993.0 30 74168.0 31 92284.0 32 115697.0 33 148271.0 34 198684.0 35 275741.0 36 401350.0 37 536130.0 38 345791.0 39 24423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.884608728692015 19.20945125905348 14.565442369782563 23.34049764247194 2 16.017268279290423 20.047243262112264 39.611178522416026 24.324309936181287 3 20.007011958735916 23.279018538721488 29.442811658560196 27.271157843982397 4 13.382318214641392 16.186191222040076 35.79250214180518 34.638988421513346 5 13.810310950316564 37.390564156086 34.07331450353458 14.725810390062858 6 33.72653284878315 35.610939720350615 16.319991725584483 14.342535705281747 7 29.764357879118243 32.159717513887976 19.700485931782463 18.37543867521132 8 26.823670151985347 35.66368151409635 18.670100650507475 18.842547683410825 9 26.2276916852391 16.715764320613825 19.82194035804339 37.23460363610368 10-11 25.50326689273013 26.46214482701582 26.53977452593693 21.494813754317118 12-13 26.161875725486055 23.52798462944002 27.487919260828935 22.822220384244986 14-15 22.68113878164555 25.217003771779755 25.586101263483474 26.515756183091217 16-17 22.413227961288207 28.494010745332428 26.44962930542442 22.643131987954945 18-19 22.422525271000417 26.990641468739174 28.650639042692717 21.936194217567696 20-21 22.786964601396384 25.98909876176566 29.147465218745356 22.076471418092602 22-23 22.578290606838355 25.861068812304655 29.082016503727797 22.478624077129194 24-25 22.567954968318798 26.134984768763168 29.108450741180008 22.188609521738023 26-27 22.201373386673733 26.38747057021766 29.08655925703192 22.324596786076686 28-29 22.17392453404889 26.798536974254834 28.94704469674082 22.080493794955462 30-31 22.32401941901891 26.308876782317085 28.918701966276434 22.448401832387567 32-33 22.146762121581432 26.31102524038964 28.98933490202461 22.55287773600432 34-35 22.67665173646747 26.381867318074082 28.847764824075504 22.093716121382936 36-37 22.72180738179773 26.119108994510214 28.787113662558234 22.37196996113382 38-39 22.363906485344273 26.52589122101338 28.81869699911514 22.29150529452721 40-41 22.711893796378668 26.41005696867626 28.62955948571334 22.24848974923173 42-43 22.364748238512945 26.394742341693405 28.522161183410482 22.718348236383168 44-45 22.455954224525318 26.50923979743649 28.629654240781356 22.405151737256837 46-47 22.926443259982822 26.897801461940162 27.965642162710147 22.210113115366877 48-49 22.80265024661637 26.932880268523036 27.8411836977283 22.423285787132297 50-51 22.813734769238494 27.1052512498132 27.82770354929076 22.25331043165755 52-53 23.047042105595384 26.987010004209456 27.65478119380499 22.311166696390174 54-55 22.74795012383104 27.03864904956398 27.761348516784402 22.452052309820584 56-57 22.848091085496083 27.21706613410257 27.57943305803917 22.35540972236218 58-59 22.994319324752936 26.87920684251574 27.58437641289638 22.542097419834946 60-61 22.63119188468446 27.073518714210586 27.918870420599646 22.37641898050531 62-63 22.557003536019753 27.147669037068695 28.042587382352906 22.252740044558642 64-65 22.483898922842975 27.158506391947963 27.863847078458264 22.493747606750798 66-67 22.520821981045657 27.10979533370117 27.66289970351278 22.70648298174039 68-69 22.648775243945426 26.920867035340095 27.91533845491026 22.515019265804213 70-71 22.732695728919953 26.959589762154145 27.686814715158974 22.62089979376692 72-73 22.679009336476295 27.035664023746353 27.62683222595847 22.658494413818882 74-75 22.50633842784174 27.466710776610704 27.420167180490473 22.606783615057083 76-77 22.732454607644023 27.085572894900857 27.596981967782252 22.58499052967287 78-79 22.724222020516443 27.17248087587122 27.53775677401225 22.565540329600086 80-81 22.86543954807072 27.311684726396614 27.339044304644627 22.48383142088804 82-83 22.788546604782116 27.380909600984587 27.401329905612183 22.42921388862112 84-85 22.69812581351831 27.433444077662738 27.31307283762412 22.555357271194826 86-87 22.777971498136925 27.41478131548757 27.246460082599654 22.560787103775848 88-89 22.727264085043956 27.464462031802505 27.053840359298242 22.7544335238553 90-91 22.81878582067458 27.284341973934634 27.201854950238726 22.695017255152063 92-93 22.759924745054384 27.083034268759786 27.485167189376085 22.671873796809745 94-95 22.72040042695376 27.044524036682738 27.467865341492658 22.767210194870852 96-97 22.786032969134833 26.886241616735614 27.44670398012315 22.881021434006403 98-99 22.5399709972486 27.154077924882987 27.176950817197298 23.129000260671116 100-101 22.588744982247107 26.72645975272027 27.667816723871603 23.016978541161023 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 424.0 1 301.5 2 271.0 3 376.5 4 308.5 5 272.0 6 549.0 7 1154.0 8 1840.5 9 2221.0 10 2125.0 11 1931.5 12 2013.0 13 2213.5 14 2478.0 15 2738.0 16 2923.0 17 3091.0 18 3334.5 19 3699.5 20 4124.0 21 4829.5 22 5845.0 23 7202.0 24 9067.0 25 11704.0 26 14982.0 27 18534.5 28 21200.5 29 24296.5 30 29007.5 31 34163.5 32 41159.5 33 45817.5 34 48556.0 35 53155.0 36 59361.0 37 65816.5 38 69838.5 39 72855.0 40 75586.5 41 79080.5 42 81459.0 43 84201.5 44 88668.5 45 93101.0 46 95647.0 47 99865.5 48 104224.5 49 102312.0 50 97410.0 51 93099.5 52 90911.0 53 90900.5 54 92107.5 55 87084.0 56 78764.0 57 72623.5 58 67619.5 59 59886.0 60 48822.0 61 40266.5 62 33552.5 63 25786.5 64 18459.5 65 13502.0 66 9907.5 67 7681.5 68 5917.0 69 4468.0 70 3257.0 71 2138.0 72 1539.0 73 1282.5 74 974.0 75 661.0 76 475.5 77 312.0 78 216.0 79 145.5 80 93.5 81 74.0 82 59.5 83 34.0 84 19.5 85 12.5 86 7.0 87 3.0 88 1.5 89 2.5 90 2.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02114234846621008 2 0.0 3 0.0 4 7.605161318780603E-5 5 0.023842180734377192 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.9012903296951507E-5 12-13 0.003441335496748223 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.93606454956721E-4 24-25 0.0 26-27 6.274258087993998E-4 28-29 0.0014259677472713633 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.605161318780603E-5 40-41 5.513741956115939E-4 42-43 0.015571567800203287 44-45 1.9012903296951507E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.5210322637561205E-4 70-71 5.703870989085453E-5 72-73 0.0 74-75 1.9012903296951507E-5 76-77 0.0 78-79 0.0 80-81 3.802580659390301E-5 82-83 0.0021864838791494237 84-85 4.563096791268362E-4 86-87 0.0 88-89 0.0 90-91 0.010970445202341021 92-93 0.002490690331900648 94-95 0.0 96-97 0.0 98-99 0.0016160967802408782 100-101 0.01914599362003017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2629793.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.20335390208574 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0951881023215 39.95315868951509 2 13.883482172144374 14.772956307957902 3 4.6657806286092125 7.447055340401759 4 2.1175833265784845 4.506501405644444 5 1.105172208428459 2.939943406388448 6 0.6854651438806423 2.1881426782455558 7 0.44933323393316577 1.673422454544043 8 0.31218248957407396 1.3287324379874919 9 0.2314368998359983 1.108189735917857 >10 1.300723303889936 12.755422210764383 >50 0.1031314124854526 3.7688655523365067 >100 0.04557946639893486 4.361953797186608 >500 0.0033660255105856427 1.2465378803584564 >1k 0.001432351281090022 1.353188026373745 >5k 1.4323512810900221E-4 0.5959300763779177 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8434 0.32070965281297803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7206 0.27401396231566516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4690 0.17834103292540515 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3160 0.12016154883673354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.605161318780604E-5 0.0 0.0 0.0 0.0 6 7.605161318780604E-5 0.0 0.0 0.0 0.0 7 7.605161318780604E-5 0.0 0.0 0.0 0.0 8 1.1407741978170905E-4 0.0 0.0 0.0 0.0 9 1.1407741978170905E-4 0.0 0.0 0.0 0.0 10-11 1.1407741978170905E-4 0.0 0.0 0.0 0.0 12-13 2.091419362664666E-4 0.0 0.0 0.0 0.0 14-15 2.6618064615732115E-4 0.0 0.0 0.0 0.0 16-17 2.6618064615732115E-4 0.0 0.0 3.802580659390302E-5 0.0 18-19 2.8519354945427265E-4 0.0 0.0 3.802580659390302E-5 0.0 20-21 3.0420645275122416E-4 0.0 0.0 3.802580659390302E-5 0.0 22-23 3.2321935604817567E-4 0.0 0.0 5.7038709890854533E-5 0.0 24-25 4.943354857207393E-4 0.0 0.0 2.091419362664666E-4 0.0 26-27 5.133483890176907E-4 0.0 0.0 3.2321935604817567E-4 0.0 28-29 5.703870989085453E-4 0.0 0.0 6.274258087993999E-4 0.0 30-31 6.084129055024483E-4 0.0 0.0 0.0013118903274896541 0.0 32-33 6.274258087993999E-4 0.0 0.0 0.002680819364870163 0.0 34-35 7.605161318780604E-4 0.0 0.0 0.0041258000154384775 0.0 36-37 9.126193582536724E-4 0.0 0.0 0.006274258087993998 0.0 38-39 9.506451648475754E-4 0.0 0.0 0.009677567778148318 0.0 40-41 9.506451648475754E-4 0.0 0.0 0.013518174244132522 0.0 42-43 0.00100768387473843 0.0 0.0 0.016826419417802087 0.0 44-45 0.0011407741978170906 0.0 0.0 0.019811445235423473 0.0 46-47 0.0011788000044109935 0.0 0.0 0.02429849041350403 0.0 48-49 0.0011788000044109935 0.0 0.0 0.028310213009160798 0.0 50-51 0.0011788000044109935 0.0 0.0 0.03226489689492671 0.0 52-53 0.0011978129077079452 0.0 0.0 0.042113580802747595 0.0 54-55 0.0012168258110048966 0.0 0.0 0.05831257441175028 1.901290329695151E-5 56-57 0.0012168258110048966 0.0 0.0 0.08145127772414026 3.802580659390302E-5 58-59 0.0012168258110048966 0.0 0.0 0.11238527138828036 3.802580659390302E-5 60-61 0.001235838714301848 0.0 0.0 0.15124764572724925 3.802580659390302E-5 62-63 0.0012548516175987995 0.0 0.0 0.17737137485726062 3.802580659390302E-5 64-65 0.0012548516175987995 0.0 0.0 0.208989833040091 3.802580659390302E-5 66-67 0.0012548516175987995 0.0 0.0 0.2648497429265345 3.802580659390302E-5 68-69 0.0012548516175987995 0.0 0.0 0.33761212384396794 3.802580659390302E-5 70-71 0.0013118903274896541 0.0 0.0 0.411705407992188 3.802580659390302E-5 72-73 0.0013499161340835572 0.0 0.0 0.49091316312728794 3.802580659390302E-5 74-75 0.0013689290373805087 0.0 0.0 0.5716229376228471 3.802580659390302E-5 76-77 0.0013689290373805087 0.0 0.0 0.6554508282591063 3.802580659390302E-5 78-79 0.0013879419406774601 0.0 0.0 0.7374915059854521 3.802580659390302E-5 80-81 0.0015590580703500237 0.0 0.0 0.8229164804986553 3.802580659390302E-5 82-83 0.0015590580703500237 0.0 0.0 0.9330772422011923 3.802580659390302E-5 84-85 0.0015970838769439268 0.0 0.0 1.0813018363042262 3.802580659390302E-5 86-87 0.0016351096835378297 0.0 0.0 1.2568289595416826 3.802580659390302E-5 88-89 0.0016351096835378297 0.0 0.0 1.467606005491687 3.802580659390302E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11350 0.0 31.944824 1 GGTATCA 7590 0.0 31.116192 1 ATCAACG 13675 0.0 26.299622 3 TCAACGC 13705 0.0 26.207388 4 CAACGCA 13965 0.0 25.71946 5 TATCAAC 14040 0.0 25.649746 2 AACGCAG 14350 0.0 25.055386 6 GTACATG 9410 0.0 22.371029 1 ACGCAGA 16415 0.0 21.759583 7 GAGTACT 8790 0.0 21.749567 12-13 TACATGG 9400 0.0 21.63201 2 CGCAGAG 16640 0.0 21.40827 8 ACATGGG 9505 0.0 20.89321 3 GTACTTT 9325 0.0 20.424555 14-15 GGACCGT 280 1.5807018E-9 20.35543 6 AGAGTAC 13630 0.0 20.159563 10-11 AGTACTT 9395 0.0 19.919254 12-13 GCAGAGT 18075 0.0 19.3933 9 GAGTACA 7465 0.0 18.52401 1 GGACCGA 565 0.0 17.653381 6 >>END_MODULE