FastQCFastQC Report
Thu 26 May 2016
SRR937905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526963
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72470.28678694543608274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.2307513010677244No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37290.1475684448090455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.14519405309852182No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25920.10257372189462213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71300.031.542361
GTATCAA103700.030.077911
GTACATG85900.026.7901271
TACATGG85250.026.4298342
ATCAACG123150.025.008183
TCAACGC124250.024.7475474
ACATGGG88050.024.6676833
TATCAAC126400.024.4442522
CAACGCA128150.024.0310125
AACGCAG131400.023.4630266
GTATACG1452.3180077E-522.9536951
GAGTACT80500.021.38922912-13
GTATAGG6800.020.9765911
ACGCAGA148200.020.7079097
CGCAGAG150000.020.396078
GTACCTG16550.020.1104891
CATGGGA43050.019.8711784
GTACTTT85600.019.86477514-15
AGAGTAC117100.019.83571410-11
TATAACG1951.0269579E-519.5014042
Produced by FastQC (version 0.11.5)