FastQCFastQC Report
Thu 26 May 2016
SRR937905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526963
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80400.3181684892101705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68790.27222400961153764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46000.18203669780681395No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30390.1202629401380234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.029.0142521
GTATCAA118800.029.0044521
CGTATAC1606.249138E-826.7182313
GTACATG90500.024.7877141
TACATGG91850.023.8917522
ATCAACG142850.023.8742313
TCAACGC143800.023.6834774
TATCAAC145650.023.5131052
CAACGCA145900.023.342595
ACATGGG89950.022.7066213
AACGCAG150650.022.6367866
GGACCGA5450.021.7872756
GAGTACT92200.020.8396312-13
AGAGTAC135100.020.55093210-11
TAAGGTG10200.020.4897985
AGTACTT96850.020.30500412-13
AGGACCG7150.019.9296845
ACGCAGA172900.019.7806197
CGCAGAG173350.019.5922628
GTACTTT98050.019.27965214-15
Produced by FastQC (version 0.11.5)