FastQCFastQC Report
Thu 26 May 2016
SRR937904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223704
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61930.2784992966689811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.22745833078503253No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32830.1476365559444962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.1417005140972No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23760.106848753251332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89150.030.3030031
GGTATCA61100.030.0472531
GTACATG79750.026.1217421
TACATGG79100.026.019312
ATCAACG106000.025.2008293
ACATGGG78750.024.9881523
TCAACGC107600.024.8288964
TATCAAC109450.024.6236062
CAACGCA109600.024.2879715
AACGCAG111800.023.7950046
TAAGGTG9800.021.3422575
GAGTACT66700.021.29343612-13
ACGCAGA126700.020.8471897
CGCAGAG128600.020.5391838
AGAGTAC102550.020.52004210-11
CATGGGG42650.020.2854984
AGTACTT71650.020.05439812-13
ATACCGT2401.4130819E-719.7937626
GTACTTT71950.019.47409814-15
CATGGGA40550.019.4603464
Produced by FastQC (version 0.11.5)