##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937904_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2223704 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.466857099685928 31.0 31.0 34.0 30.0 34.0 2 31.56587432500009 33.0 31.0 34.0 30.0 34.0 3 31.6148817468512 33.0 31.0 34.0 30.0 34.0 4 35.22929220795574 37.0 35.0 37.0 33.0 37.0 5 35.22131497717322 37.0 35.0 37.0 33.0 37.0 6 35.2713103902318 37.0 35.0 37.0 33.0 37.0 7 35.17072056352824 37.0 35.0 37.0 32.0 37.0 8 35.16646190320294 37.0 35.0 37.0 32.0 37.0 9 36.70070207185849 39.0 37.0 39.0 32.0 39.0 10-11 36.58787702859733 39.0 37.0 39.0 32.0 39.0 12-13 36.46833593859614 39.0 37.0 39.0 32.0 39.0 14-15 37.544411711270925 40.0 37.0 41.0 32.0 41.0 16-17 37.434670486719455 40.0 37.0 41.0 32.0 41.0 18-19 37.319177372528 40.0 37.0 41.0 32.0 41.0 20-21 37.303250117821435 40.0 37.0 41.0 31.5 41.0 22-23 37.306791281573446 40.0 37.0 41.0 32.0 41.0 24-25 37.21725530915985 40.0 37.0 41.0 31.0 41.0 26-27 37.09291479441508 40.0 37.0 41.0 31.0 41.0 28-29 36.92560070944694 39.0 36.0 41.0 30.5 41.0 30-31 36.674127491788475 39.0 36.0 41.0 30.0 41.0 32-33 36.52914596547022 39.0 36.0 41.0 30.0 41.0 34-35 36.492959944309135 39.0 36.0 41.0 30.0 41.0 36-37 36.30268102229434 39.0 35.0 41.0 30.0 41.0 38-39 36.10209047607056 39.0 35.0 41.0 29.0 41.0 40-41 35.84522805193497 39.0 35.0 40.5 28.0 41.0 42-43 35.55505791238402 39.0 35.0 40.0 27.0 41.0 44-45 35.30106345089094 38.0 35.0 40.0 26.0 41.0 46-47 34.93906585588729 38.0 34.0 40.0 24.5 41.0 48-49 34.56917827192828 38.0 34.0 40.0 24.0 41.0 50-51 33.680902224396775 37.5 32.5 39.5 22.5 40.5 52-53 33.78577364613275 38.0 33.0 39.5 23.0 40.5 54-55 34.003332502887076 38.0 33.0 40.0 22.5 41.0 56-57 33.70264320251256 38.0 33.0 40.0 20.5 41.0 58-59 33.36521317585434 37.5 33.0 40.0 18.5 41.0 60-61 32.9526227411562 37.0 32.0 40.0 17.5 41.0 62-63 32.53054992930714 36.0 31.5 39.0 13.5 41.0 64-65 32.046633904512476 36.0 31.0 39.0 9.5 40.5 66-67 31.50269617718905 35.0 30.5 39.0 7.5 40.0 68-69 30.980659521231246 35.0 30.0 38.0 4.5 40.0 70-71 30.46066990031047 35.0 29.0 37.5 2.0 40.0 72-73 29.951653862204683 34.0 29.0 37.0 2.0 39.0 74-75 29.365749892971365 34.0 28.5 36.0 2.0 39.0 76-77 28.783549429240583 34.0 27.5 36.0 2.0 38.5 78-79 28.209720808165116 34.0 26.5 35.0 2.0 37.5 80-81 27.617196128621437 33.0 26.0 35.0 2.0 37.0 82-83 27.013196900306873 33.0 25.0 35.0 2.0 36.0 84-85 26.420071871076367 32.0 24.5 35.0 2.0 36.0 86-87 25.792360628932627 32.0 22.0 35.0 2.0 35.5 88-89 25.165398812072112 31.5 19.5 34.0 2.0 35.0 90-91 24.518199364663644 31.0 16.5 34.0 2.0 35.0 92-93 23.768277387637923 31.0 7.0 34.0 2.0 35.0 94-95 22.90797853491292 30.5 2.0 34.0 2.0 35.0 96-97 21.924035303259785 30.0 2.0 34.0 2.0 35.0 98-99 20.65334235132014 29.0 2.0 34.0 2.0 35.0 100-101 17.567948791745664 22.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04416331652272021 1101 2 0.029227719575970923 1101 3 -0.035502539694839186 1101 4 -0.060730707559592645 1101 5 -0.16876379870698344 1101 6 -0.13720335494985392 1101 7 -0.10807327809457234 1101 8 -0.047650267052880224 1101 9 0.05975066109603944 1101 10-11 0.06393697119940356 1101 12-13 0.11757644186597105 1101 14-15 0.2790466800991709 1101 16-17 0.21809961055545557 1101 18-19 0.28198847019329776 1101 20-21 0.2215897445573347 1101 22-23 0.0867551342580839 1101 24-25 0.17993938522592856 1101 26-27 0.16356147233064178 1101 28-29 0.20145580624863157 1101 30-31 0.10234105741099597 1101 32-33 0.17815341290242515 1101 34-35 0.24746013712213966 1101 36-37 0.3207882637319912 1101 38-39 0.2607393306260448 1101 40-41 0.32234409368056305 1101 42-43 0.23059065945931678 1101 44-45 0.28195587112528386 1101 46-47 0.37610760001963683 1101 48-49 0.5246065510260394 1101 50-51 0.3695408848806565 1101 52-53 0.49111586905880245 1101 54-55 0.4465623819418809 1101 56-57 0.46675300396430686 1101 58-59 0.572640686586432 1101 60-61 0.5547691470130758 1101 62-63 0.6304054208693088 1101 64-65 0.7246126475555883 1101 66-67 0.7395231526681165 1101 68-69 0.7238614410987836 1101 70-71 0.8292308286364758 1101 72-73 0.9041078742891564 1101 74-75 1.0223490806391844 1101 76-77 0.8730220441723411 1101 78-79 1.221742248724766 1101 80-81 1.3895881380427362 1101 82-83 1.4815445149347894 1101 84-85 1.64896946124885 1101 86-87 1.8519593808997357 1101 88-89 1.8855604248174487 1101 90-91 1.9424169305248142 1101 92-93 2.0492284536490892 1101 94-95 2.293582009171825 1101 96-97 2.2195680007933625 1101 98-99 2.5831673112856706 1101 100-101 3.231635743699343 1102 1 0.01780784115423728 1102 2 0.023367473642114334 1102 3 -0.051320272324730354 1102 4 0.006038371595259662 1102 5 -0.06265987401260276 1102 6 -0.07310311349479548 1102 7 -0.021338472764846017 1102 8 -8.836925982791399E-5 1102 9 0.12038166207302936 1102 10-11 0.16699311280280682 1102 12-13 0.09893100747773786 1102 14-15 0.1360464633533951 1102 16-17 0.20790117894724602 1102 18-19 0.09423849148199537 1102 20-21 0.19754142790810647 1102 22-23 0.14176748824409202 1102 24-25 0.06366842239724235 1102 26-27 0.14501250747157712 1102 28-29 0.22933635041787426 1102 30-31 0.31546762373278625 1102 32-33 0.2519856312660025 1102 34-35 0.20081577900684522 1102 36-37 0.27700188899672185 1102 38-39 0.4491483760800037 1102 40-41 0.47449694693530375 1102 42-43 0.4492337131757722 1102 44-45 0.43926680294769227 1102 46-47 0.4094621849907156 1102 48-49 0.47720214382704285 1102 50-51 0.26806540468803775 1102 52-53 0.31792971530743586 1102 54-55 0.49291635042362003 1102 56-57 0.5395931231496363 1102 58-59 0.6150538584509277 1102 60-61 0.6202721535268267 1102 62-63 0.683188576596244 1102 64-65 0.776150070996394 1102 66-67 0.9849059901960366 1102 68-69 0.8183425207098871 1102 70-71 0.724113116844002 1102 72-73 0.8207510067509141 1102 74-75 0.8662798915156138 1102 76-77 0.9374379862492894 1102 78-79 1.047297870700774 1102 80-81 1.2423634715624274 1102 82-83 1.4187353954968813 1102 84-85 1.5423522532562295 1102 86-87 1.6751307993392928 1102 88-89 1.6979133905393056 1102 90-91 1.8141012074042635 1102 92-93 1.97940099610582 1102 94-95 2.275366926988287 1102 96-97 2.351354076244178 1102 98-99 2.6532183819273953 1102 100-101 2.890460037172865 1103 1 -0.012799955171722388 1103 2 -0.05774723409716742 1103 3 0.05502276885953705 1103 4 -0.011986597395242882 1103 5 0.07332477146407257 1103 6 0.11200443516118952 1103 7 0.06581788514668574 1103 8 -0.038224216240386966 1103 9 -0.11012764991196633 1103 10-11 0.028277499850076993 1103 12-13 0.09131731274925414 1103 14-15 0.19573378508683703 1103 16-17 0.1840003286474996 1103 18-19 0.16770727694498078 1103 20-21 0.19420676487122535 1103 22-23 0.06532786845810534 1103 24-25 0.0036360502061185684 1103 26-27 -0.0758732654109977 1103 28-29 -0.062185101963514455 1103 30-31 -0.050852063898531696 1103 32-33 -0.03340319790464008 1103 34-35 0.06844004792365155 1103 36-37 0.18986012229778026 1103 38-39 0.11117484682347367 1103 40-41 0.03034411527576708 1103 42-43 -0.11855788522939292 1103 44-45 0.1777812347988572 1103 46-47 0.035463903454910906 1103 48-49 -0.062451569903004156 1103 50-51 0.0026767506119398377 1103 52-53 0.12204953783768957 1103 54-55 0.022448710941780803 1103 56-57 0.11517769787796084 1103 58-59 0.21705715581121154 1103 60-61 0.36274803615562234 1103 62-63 0.5551751105564691 1103 64-65 0.4808698588122411 1103 66-67 0.6526002588692315 1103 68-69 0.6730186110160439 1103 70-71 0.7500109966266209 1103 72-73 0.592162215542789 1103 74-75 0.6466580982834991 1103 76-77 0.5867835513326902 1103 78-79 0.8247349330912748 1103 80-81 0.8897182877283747 1103 82-83 0.9076537501765252 1103 84-85 1.15074643829006 1103 86-87 1.3483823533668726 1103 88-89 1.4493174458306122 1103 90-91 1.56015149303677 1103 92-93 1.5261192040105485 1103 94-95 1.8769803852122493 1103 96-97 2.011883552016169 1103 98-99 2.260980704016106 1103 100-101 2.824607737800765 1104 1 0.20942071024819242 1104 2 0.22672798914828363 1104 3 0.21664760834502417 1104 4 0.19400796821758348 1104 5 0.17878415760544897 1104 6 0.32550754024435946 1104 7 0.3570643380955332 1104 8 0.2343812907101679 1104 9 0.31682558800741134 1104 10-11 0.2951650767643059 1104 12-13 0.2897428694125992 1104 14-15 0.400646536969262 1104 16-17 0.462499950590356 1104 18-19 0.396579953786258 1104 20-21 0.50848754186962 1104 22-23 0.34201021048527736 1104 24-25 0.3530903377380028 1104 26-27 0.25260561009267235 1104 28-29 0.37046744581720503 1104 30-31 0.488595606404175 1104 32-33 0.3847885409812619 1104 34-35 0.6259532209292971 1104 36-37 0.5819260350132822 1104 38-39 0.6733562144562768 1104 40-41 0.6790426203522557 1104 42-43 0.6482525685178473 1104 44-45 0.723493334200846 1104 46-47 0.6608762512242308 1104 48-49 0.8557090124524933 1104 50-51 0.6971002601621805 1104 52-53 0.6120975152073527 1104 54-55 0.6173806311574666 1104 56-57 0.908886377067212 1104 58-59 0.9203175054329549 1104 60-61 0.897627495410994 1104 62-63 0.8633098163994859 1104 64-65 1.0078532577279162 1104 66-67 1.1254965355225046 1104 68-69 0.9603887491224334 1104 70-71 1.0227048725924135 1104 72-73 1.0710283169427335 1104 74-75 1.1182620856311196 1104 76-77 1.2155885195640934 1104 78-79 1.366477555323197 1104 80-81 1.595455919507593 1104 82-83 1.5433119260434012 1104 84-85 1.7123161669738316 1104 86-87 1.8713123921459989 1104 88-89 1.9714372304609427 1104 90-91 1.965090337745245 1104 92-93 2.010713190787893 1104 94-95 2.299960704609088 1104 96-97 2.5679537536400936 1104 98-99 2.9683334977060447 1104 100-101 3.4584119464765166 1105 1 0.004784739378902003 1105 2 0.17081180114903916 1105 3 0.011479691299854267 1105 4 -0.06877798598027596 1105 5 -0.02910785391335935 1105 6 -0.01817285084398179 1105 7 -0.016378283779459935 1105 8 -0.10121149925476658 1105 9 0.07173697195846529 1105 10-11 -0.005206755505682281 1105 12-13 0.07865640436973109 1105 14-15 0.13643966177632194 1105 16-17 0.03972755565635566 1105 18-19 0.06031424875375535 1105 20-21 0.09519792361641066 1105 22-23 0.11737407302786096 1105 24-25 -0.025376493682649937 1105 26-27 0.07572770126837014 1105 28-29 0.16611928117664831 1105 30-31 0.21674504958335206 1105 32-33 0.16560633471335962 1105 34-35 0.32402101321005006 1105 36-37 0.2702692454239255 1105 38-39 0.20706986511643777 1105 40-41 0.2124653097055358 1105 42-43 0.13459537103999253 1105 44-45 0.2594683555733468 1105 46-47 0.3085725898443812 1105 48-49 0.35579268512499596 1105 50-51 0.261446564568665 1105 52-53 0.12210100598581874 1105 54-55 0.28875412754187124 1105 56-57 0.21991909219874373 1105 58-59 0.37078239988802153 1105 60-61 0.43496860252034253 1105 62-63 0.6144301846501108 1105 64-65 0.5166440626691298 1105 66-67 0.5044132179856042 1105 68-69 0.5570723959990183 1105 70-71 0.6652485899862555 1105 72-73 0.7029274316782832 1105 74-75 0.8310445313820765 1105 76-77 0.8777402904337386 1105 78-79 1.1372177770419007 1105 80-81 1.1953150263377808 1105 82-83 1.2580605533951008 1105 84-85 1.5287346636068584 1105 86-87 1.7247511244406475 1105 88-89 1.6558102167323128 1105 90-91 1.686543821670881 1105 92-93 1.6979107923227197 1105 94-95 1.9703896967988825 1105 96-97 1.6579982957713675 1105 98-99 1.7652333557780047 1105 100-101 2.389500622598149 1106 1 -0.12466649333174828 1106 2 -0.16298517932172985 1106 3 -0.1611862748472781 1106 4 -0.11447207281783278 1106 5 -0.18044556242445964 1106 6 -0.23964200418596704 1106 7 -0.12463043771688831 1106 8 -0.16406689125790308 1106 9 -0.07150196916987284 1106 10-11 -0.5326785490073931 1106 12-13 -0.5772492715733577 1106 14-15 -0.6915490740403527 1106 16-17 -0.495621946564647 1106 18-19 -0.13160857595025988 1106 20-21 -0.0571716144063501 1106 22-23 -0.32199820310636795 1106 24-25 -0.49029062043219795 1106 26-27 -0.38783658643430385 1106 28-29 -0.12874648623787976 1106 30-31 0.05632485262829334 1106 32-33 0.07532091882014669 1106 34-35 0.061872862135906814 1106 36-37 -0.3970508109557116 1106 38-39 -0.44023060358397714 1106 40-41 -0.1269693005679784 1106 42-43 0.06938847000621706 1106 44-45 0.018216353501145477 1106 46-47 0.15652539845709157 1106 48-49 0.3025253540056738 1106 50-51 0.2150832298003067 1106 52-53 0.2715652206383865 1106 54-55 0.47395071028053337 1106 56-57 0.23959865121840096 1106 58-59 0.015550802738168557 1106 60-61 0.29170483983365614 1106 62-63 0.09829416998351093 1106 64-65 0.3038886108006693 1106 66-67 0.5573367838666918 1106 68-69 0.29101858606329856 1106 70-71 0.33844521703006336 1106 72-73 0.2624633405997123 1106 74-75 0.49193580414884863 1106 76-77 0.8638913955402252 1106 78-79 0.6528177928422885 1106 80-81 0.7435098184545588 1106 82-83 0.6935116905560967 1106 84-85 0.8171438232423078 1106 86-87 0.8808251465980277 1106 88-89 0.9815062279078859 1106 90-91 1.2238249348937522 1106 92-93 1.4909932108392816 1106 94-95 1.5561855466877041 1106 96-97 1.6629305796242981 1106 98-99 1.6465177806771791 1106 100-101 2.0916504631396577 1107 1 -0.06752438731746224 1107 2 -0.010577034298119514 1107 3 -0.09900409612374617 1107 4 -0.025527183121056396 1107 5 -0.034935057891395616 1107 6 0.006552993083147385 1107 7 0.06987913434370085 1107 8 0.06513020058687857 1107 9 0.0577239338208031 1107 10-11 -0.051915957678211555 1107 12-13 0.022046505152609086 1107 14-15 0.1748335363675011 1107 16-17 0.0060120934018357275 1107 18-19 0.10428281515058302 1107 20-21 0.1914004365287525 1107 22-23 0.13968578959266154 1107 24-25 0.23525954100679058 1107 26-27 0.24387675929622787 1107 28-29 0.13186179645687446 1107 30-31 0.1159880680380212 1107 32-33 0.18114554532146343 1107 34-35 0.2838216303387 1107 36-37 0.2904905681807364 1107 38-39 0.3500602012073628 1107 40-41 0.32632425067133397 1107 42-43 0.3236122939803039 1107 44-45 0.22515629862584063 1107 46-47 0.4085017384436327 1107 48-49 0.37834581442602655 1107 50-51 0.3281902489894932 1107 52-53 0.392627563236708 1107 54-55 0.5647443822369311 1107 56-57 0.6753835296053197 1107 58-59 0.3857925373730282 1107 60-61 0.6977995507081829 1107 62-63 0.7876304110786165 1107 64-65 0.5636575743827308 1107 66-67 0.9619356758447459 1107 68-69 1.0812034951391816 1107 70-71 1.1345346002569343 1107 72-73 1.1405929982675005 1107 74-75 1.0881241217388826 1107 76-77 1.15300353206106 1107 78-79 1.3048775349261987 1107 80-81 1.4755249166753508 1107 82-83 1.606614296864933 1107 84-85 1.6148561136371988 1107 86-87 1.8259363592307203 1107 88-89 1.7814308602036242 1107 90-91 1.8813777741060953 1107 92-93 2.1986553794976906 1107 94-95 2.388005981891599 1107 96-97 2.453646576838686 1107 98-99 2.640187108127389 1107 100-101 3.1848667929966936 1108 1 -0.044214914274512296 1108 2 -0.0843325279668008 1108 3 -0.11306897580696074 1108 4 -0.18559419500044072 1108 5 -0.08702917912197705 1108 6 0.04766195265845141 1108 7 -0.005188312677752549 1108 8 -0.015751832396887266 1108 9 -0.0038823931449556426 1108 10-11 -0.011290549732514421 1108 12-13 0.060177461159746315 1108 14-15 -0.024234171739195176 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0.07704811823302293 2316 8 0.011895733792265162 2316 9 -0.3461009486088358 2316 10-11 -0.2556829095415125 2316 12-13 -0.3695339066244472 2316 14-15 -0.4157398968823287 2316 16-17 -0.3787826895508317 2316 18-19 -0.2511117475560525 2316 20-21 -0.37631178834683965 2316 22-23 -0.4335161042301934 2316 24-25 -0.3182230688354153 2316 26-27 -0.494971759292838 2316 28-29 -0.25084467809274713 2316 30-31 -0.35541779509689775 2316 32-33 -0.30781285210786535 2316 34-35 -0.31646204020058377 2316 36-37 -0.3630669424042665 2316 38-39 -0.4196581671162818 2316 40-41 -0.32487300138096487 2316 42-43 -0.6429799602939852 2316 44-45 -0.6217336418442443 2316 46-47 -0.6691413970589508 2316 48-49 -0.8999281477072856 2316 50-51 -0.9122634163244356 2316 52-53 -0.9378953135378296 2316 54-55 -0.7238569700053219 2316 56-57 -0.7860950855306115 2316 58-59 -0.7987111276967269 2316 60-61 -0.8386313892198416 2316 62-63 -0.8497440828682841 2316 64-65 -0.8734050467892089 2316 66-67 -0.7155735906041194 2316 68-69 -0.7598448588757911 2316 70-71 -0.844062832973357 2316 72-73 -0.6505334307678616 2316 74-75 -0.8542938720199977 2316 76-77 -1.02408239563378 2316 78-79 -1.2485623442559124 2316 80-81 -1.2465820345042609 2316 82-83 -1.4537232265148425 2316 84-85 -1.6327756921360823 2316 86-87 -1.9256333820900693 2316 88-89 -1.888185748105169 2316 90-91 -1.860267160967716 2316 92-93 -1.8288610290757852 2316 94-95 -1.880248556104501 2316 96-97 -2.2364804431006604 2316 98-99 -2.6098849235567876 2316 100-101 -2.2680022045664376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11247.0 3 2731.0 4 2814.0 5 3030.0 6 3481.0 7 4063.0 8 5005.0 9 6141.0 10 7693.0 11 9399.0 12 11348.0 13 13822.0 14 15386.0 15 18179.0 16 20851.0 17 24569.0 18 22817.0 19 19911.0 20 20561.0 21 21874.0 22 23982.0 23 26776.0 24 30130.0 25 33598.0 26 38546.0 27 43990.0 28 51506.0 29 60878.0 30 71610.0 31 86288.0 32 106490.0 33 134084.0 34 173527.0 35 229778.0 36 304910.0 37 350285.0 38 198124.0 39 14279.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01426454852623 18.30486185167451 13.910010772423858 23.770862827375407 2 15.45121175626852 19.22008272328295 40.813881634438864 24.514823886009662 3 19.75274671988171 23.128200108529096 28.97059294506741 28.148460226521788 4 12.767317298693099 15.474172051013161 36.35348375675157 35.40502689354217 5 13.853186801290912 37.047047069622266 34.15322717052279 14.946538958564028 6 33.67470240630492 35.00443725709454 16.806139415057288 14.514720921543256 7 29.790731998602926 31.92257512083554 19.904908908593125 18.381783971968407 8 26.579161215040674 35.18109463992677 19.13954524988541 19.100198895147148 9 26.327903874236057 16.708972579278516 20.003605617552147 36.95951792893328 10-11 25.409012609757355 26.214170270033954 26.793683673478586 21.58313344673011 12-13 26.08719951755141 23.351205805969492 27.592056905856722 22.969537770622377 14-15 22.80327086050168 24.975183708163563 25.682614218602858 26.538931212731903 16-17 22.45509980500023 28.33425042260465 26.440179416010167 22.770470356384948 18-19 22.460836593054534 26.8214614591339 28.618628503989065 22.099073443822505 20-21 22.84373503102282 25.949351402526975 28.910950160262406 22.2959634061878 22-23 22.559246597258337 25.851981075699904 28.97069149046439 22.618080836577366 24-25 22.468348725972568 26.0790770365016 28.97048074245187 22.482093495073965 26-27 22.171161789960973 26.312194644881615 28.943062572828342 22.573580992329067 28-29 22.19359447606943 26.76009933700213 28.7832794732074 22.263026713721036 30-31 22.309089204535724 26.235636561627008 28.7266964565002 22.72857777733707 32-33 22.06854402262941 26.23852061483599 28.91627572924843 22.776659633286165 34-35 22.638921221466045 26.362191820460186 28.704154627867872 22.294732330205893 36-37 22.638060254716848 26.06113618982854 28.627817863167092 22.67298569228752 38-39 22.303629226854504 26.454815695332577 28.68150843106605 22.56004664674687 40-41 22.66688173971265 26.377139597576104 28.548446179847627 22.40753248286362 42-43 22.3581762270334 26.261957593466157 28.47488972967322 22.904976449827227 44-45 22.3858081335546 26.47345052483813 28.541798340932417 22.598943000674847 46-47 22.802789070409766 26.715897309245157 27.98086237826322 22.500451242081855 48-49 22.65824057101056 26.796859189520895 27.877870591432767 22.66702964803578 50-51 22.67391026091722 27.01499647555332 27.843151306028336 22.46794195750112 52-53 22.89370573419954 26.867043424855897 27.736464595811334 22.502786245133226 54-55 22.602216493451476 26.913674836174184 27.81491964197186 22.669189028402485 56-57 22.632032108161397 27.075244041670683 27.672834127356726 22.619889722811195 58-59 22.843456272026607 26.70002551916178 27.70038567043883 22.75613253837278 60-61 22.507875534121606 26.952641367639664 27.957087349945848 22.582395748292882 62-63 22.433141630965096 26.96961963099528 28.15285671518807 22.44438202285156 64-65 22.361865533921094 27.007993677866764 27.93721029563362 22.692930492578522 66-67 22.388839090307194 26.991977685029017 27.71709709837344 22.902086126290357 68-69 22.5429671704799 26.740419607829885 28.01694920981405 22.699664011876163 70-71 22.607772802422893 26.7950757277949 27.75880825169504 22.83834321808717 72-73 22.495368395866468 26.91771391725228 27.748913096619667 22.83800459026159 74-75 22.36254900816553 27.277143386947817 27.638724004352127 22.72158360053453 76-77 22.611154610951818 26.94839541918512 27.60965058048816 22.8307993893749 78-79 22.62483383277381 26.95804699997747 27.621949845661625 22.795169321587096 80-81 22.71600337597157 27.146673167469732 27.417196997313916 22.720126459244785 82-83 22.687351051456478 27.153368762971574 27.52435046101257 22.63492972455938 84-85 22.57190798859014 27.21095285110171 27.434207814306653 22.782931346001494 86-87 22.648342966537186 27.18424271123119 27.387509807980965 22.779904514250664 88-89 22.675788356665137 27.204903452851802 27.15563010518181 22.963678085301247 90-91 22.67301693868976 27.02566430596656 27.37847388454385 22.922844870799825 92-93 22.725914912310344 26.805866252154225 27.574651663118534 22.8935671724169 94-95 22.645412747620526 26.704752687547067 27.6286304329069 23.021204131925508 96-97 22.622031517369024 26.563471258903615 27.596026139432084 23.218471084295274 98-99 22.490087150166353 26.83497839664512 27.29038707597347 23.384547377215057 100-101 22.983223459180856 25.82502472703404 28.008977642166087 23.18277417161902 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2932.0 1 1684.5 2 417.5 3 383.5 4 331.0 5 349.5 6 519.5 7 897.0 8 1438.5 9 1721.5 10 1604.0 11 1535.5 12 1658.5 13 1825.0 14 2065.5 15 2198.5 16 2228.5 17 2440.5 18 2715.5 19 3006.5 20 3422.0 21 3973.5 22 4859.0 23 6016.0 24 7559.0 25 9709.5 26 12480.0 27 15402.0 28 17611.0 29 20550.0 30 24580.0 31 28899.0 32 34707.0 33 38488.5 34 41073.5 35 45087.0 36 50132.0 37 55246.5 38 58246.5 39 60858.0 40 63938.5 41 66854.5 42 68749.0 43 70998.0 44 74495.5 45 78185.0 46 80371.5 47 83608.5 48 87199.5 49 85990.5 50 82275.5 51 78855.5 52 76726.5 53 76685.0 54 76945.5 55 72800.5 56 66214.5 57 61223.0 58 57226.5 59 51245.0 60 42329.0 61 34922.0 62 29180.0 63 22492.0 64 16400.0 65 12199.0 66 9015.0 67 6969.0 68 5382.5 69 4024.5 70 3030.0 71 2127.5 72 1500.5 73 1188.5 74 885.0 75 587.5 76 441.0 77 324.5 78 222.5 79 150.5 80 95.5 81 63.5 82 47.5 83 32.5 84 26.0 85 23.0 86 12.5 87 7.0 88 7.5 89 6.5 90 8.0 91 7.5 92 6.0 93 4.0 94 3.0 95 3.0 96 2.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3535092800120879 2 0.3001298734004166 3 0.3052114849818141 4 0.29810622277065657 5 0.2999050233304433 6 0.22444533984738976 7 0.21491169688052006 8 0.22246665923162434 9 0.22233174918964035 10-11 0.2224216892176297 12-13 0.22583941028122448 14-15 0.2203305835668776 16-17 0.2125732561527973 18-19 0.20562538899062105 20-21 0.2037591334098423 22-23 0.21396732658663203 24-25 0.21036972546705857 26-27 0.21916136320301624 28-29 0.22458024988937378 30-31 0.2270985706730752 32-33 0.22478261495234977 34-35 0.22527728510629114 36-37 0.21095433564898924 38-39 0.20106093257016225 40-41 0.19350597021905794 42-43 0.20740170454341045 44-45 0.21075197058601325 46-47 0.21713771257325615 48-49 0.22669384054712316 50-51 0.22107258879778963 52-53 0.23458607800318745 54-55 0.21963354834996027 56-57 0.1891438788615751 58-59 0.17104344822872108 60-61 0.18761489838575637 62-63 0.1812516414055108 64-65 0.1888740587776071 66-67 0.1892113338825671 68-69 0.1857936128189723 70-71 0.18925630389656178 72-73 0.19968934714332484 74-75 0.20499580879469573 76-77 0.20985257030611987 78-79 0.20479344373171968 80-81 0.20196033285005557 82-83 0.18831193360267373 84-85 0.18188122160143616 86-87 0.1893462439245511 88-89 0.20018401729726618 90-91 0.19681126624766604 92-93 0.19017818918345247 94-95 0.19132492454031652 96-97 0.20018401729726618 98-99 0.20184790781506892 100-101 0.1784185305238467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2223704.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.59357914494223 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3130713281411 45.103300716208004 2 12.446888632570612 14.337217311364746 3 3.9432705370873866 6.813211913029836 4 1.7281165809135368 3.981136762981229 5 0.9572770687728589 2.7566506312003978 6 0.559663769868976 1.9339823774703362 7 0.37607514073518544 1.516165936766403 8 0.2609886309745225 1.2025015499169027 9 0.20522969022619295 1.0637921166240798 >10 1.0875529732032385 11.649652625838126 >50 0.08203094074574828 3.2265425768882636 >100 0.03538619998892054 3.6204195332788465 >500 0.003121759138130946 1.1605206869467972 >1k 0.0011706596767988822 1.1291917075592794 >5k 1.5608795690651766E-4 0.5057135539268153 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6193 0.2784992966689811 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5058 0.22745833078503253 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3283 0.1476365559444962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3151 0.1417005140972 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2376 0.106848753251332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.497001399466836E-5 0.0 0.0 0.0 0.0 8 4.497001399466836E-5 0.0 0.0 0.0 0.0 9 4.497001399466836E-5 0.0 0.0 0.0 0.0 10-11 4.497001399466836E-5 0.0 0.0 4.497001399466836E-5 0.0 12-13 8.994002798933672E-5 0.0 0.0 4.497001399466836E-5 0.0 14-15 8.994002798933672E-5 0.0 0.0 4.497001399466836E-5 0.0 16-17 1.1242503498667088E-4 0.0 0.0 1.3491004198400505E-4 4.497001399466836E-5 18-19 1.5739504898133923E-4 0.0 0.0 2.023650629760076E-4 4.497001399466836E-5 20-21 1.7988005597867343E-4 0.0 0.0 2.4733507697067593E-4 4.497001399466836E-5 22-23 1.7988005597867343E-4 0.0 0.0 3.5976011195734686E-4 4.497001399466836E-5 24-25 1.7988005597867343E-4 0.0 0.0 5.621251749333544E-4 4.497001399466836E-5 26-27 1.7988005597867343E-4 0.0 0.0 7.195202239146937E-4 4.497001399466836E-5 28-29 2.47335076970676E-4 0.0 0.0 9.89340307882704E-4 4.497001399466836E-5 30-31 3.147900979626785E-4 2.248500699733418E-5 0.0 0.002226015692736084 4.497001399466836E-5 32-33 3.147900979626785E-4 4.497001399466836E-5 0.0 0.0033502660426027926 4.497001399466836E-5 34-35 3.5976011195734686E-4 4.497001399466836E-5 0.0 0.004541971413461503 4.497001399466836E-5 36-37 3.5976011195734686E-4 4.497001399466836E-5 0.0 0.0064981670222295776 4.497001399466836E-5 38-39 4.047301259520152E-4 4.497001399466836E-5 0.0 0.009960858099819041 4.497001399466836E-5 40-41 4.047301259520152E-4 4.497001399466836E-5 0.0 0.013333609149419168 4.497001399466836E-5 42-43 5.171551609386861E-4 4.497001399466836E-5 0.0 0.01603180998909927 6.745502099200254E-5 44-45 6.29580195925357E-4 4.497001399466836E-5 0.0 0.018302795695830022 8.994002798933672E-5 46-47 7.195202239146937E-4 4.497001399466836E-5 0.0 0.02194536682939816 8.994002798933672E-5 48-49 7.195202239146937E-4 4.497001399466836E-5 0.0 0.02518320783701428 8.994002798933672E-5 50-51 7.195202239146937E-4 4.497001399466836E-5 0.0 0.02846601885862507 8.994002798933672E-5 52-53 7.195202239146937E-4 4.497001399466836E-5 0.0 0.036605591391660044 8.994002798933672E-5 54-55 7.195202239146937E-4 8.994002798933672E-5 0.0 0.051355755981911264 8.994002798933672E-5 56-57 8.094602519040304E-4 8.994002798933672E-5 0.0 0.07213190244744805 8.994002798933672E-5 58-59 8.094602519040304E-4 8.994002798933672E-5 0.0 0.10248666189384918 8.994002798933672E-5 60-61 8.094602519040304E-4 8.994002798933672E-5 0.0 0.1402165036353759 1.3491004198400505E-4 62-63 8.094602519040304E-4 8.994002798933672E-5 0.0 0.16382576098257684 1.3491004198400505E-4 64-65 8.094602519040304E-4 8.994002798933672E-5 0.0 0.19550713584182067 1.3491004198400505E-4 66-67 8.544302658986988E-4 8.994002798933672E-5 0.0 0.25167468332116144 1.3491004198400505E-4 68-69 8.544302658986988E-4 8.994002798933672E-5 0.0 0.32756158193716434 1.3491004198400505E-4 70-71 8.994002798933671E-4 8.994002798933672E-5 0.0 0.40385321067911917 1.3491004198400505E-4 72-73 9.443702938880355E-4 8.994002798933672E-5 0.0 0.4892062972409997 1.3491004198400505E-4 74-75 9.443702938880355E-4 8.994002798933672E-5 0.0 0.5736374985159896 1.3491004198400505E-4 76-77 9.443702938880355E-4 8.994002798933672E-5 0.0 0.6618461809665315 1.3491004198400505E-4 78-79 9.668553008853695E-4 8.994002798933672E-5 0.0 0.7446584617377133 1.3491004198400505E-4 80-81 0.0011692203638613773 8.994002798933672E-5 0.0 0.83257483909729 1.3491004198400505E-4 82-83 0.0011692203638613773 8.994002798933672E-5 0.0 0.9473158298046862 1.3491004198400505E-4 84-85 0.0011917053708587115 8.994002798933672E-5 0.0 1.1148066469278286 1.3491004198400505E-4 86-87 0.0012366753848533799 8.994002798933672E-5 0.0 1.303838100754417 1.3491004198400505E-4 88-89 0.001259160391850714 8.994002798933672E-5 0.0 1.535838402952911 1.3491004198400505E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8915 0.0 30.303003 1 GGTATCA 6110 0.0 30.047253 1 GTACATG 7975 0.0 26.121742 1 TACATGG 7910 0.0 26.01931 2 ATCAACG 10600 0.0 25.200829 3 ACATGGG 7875 0.0 24.988152 3 TCAACGC 10760 0.0 24.828896 4 TATCAAC 10945 0.0 24.623606 2 CAACGCA 10960 0.0 24.287971 5 AACGCAG 11180 0.0 23.795004 6 TAAGGTG 980 0.0 21.342257 5 GAGTACT 6670 0.0 21.293436 12-13 ACGCAGA 12670 0.0 20.847189 7 CGCAGAG 12860 0.0 20.539183 8 AGAGTAC 10255 0.0 20.520042 10-11 CATGGGG 4265 0.0 20.285498 4 AGTACTT 7165 0.0 20.054398 12-13 ATACCGT 240 1.4130819E-7 19.793762 6 GTACTTT 7195 0.0 19.474098 14-15 CATGGGA 4055 0.0 19.460346 4 >>END_MODULE