FastQCFastQC Report
Thu 26 May 2016
SRR937904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223704
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72180.32459356101351616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63030.28344599820839467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39890.17938538582473207No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28340.1274450196608901No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22550.10140738155797714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.029.9285091
GTATCAA109000.028.7784081
GTACATG78200.026.2558651
TACATGG76950.025.6214222
TGCACCG4500.024.2816985
ATCAACG129150.024.1676643
TCAACGC129100.024.1034284
CAACGCA131300.023.7719255
TATCAAC131600.023.7177352
ACATGGG79400.023.6940023
TAAGGTG10450.023.640185
AACGCAG134250.023.3516
CATGGGG39650.022.1662524
GAGTACT79750.021.62126212-13
GTAAGGT10150.021.530574
ACGCAGA148850.021.0301177
AGAGTAC116200.021.01174710-11
CGCAGAG149600.020.8929338
GTACTTT84050.020.344214-15
AGTACTT83700.020.03338812-13
Produced by FastQC (version 0.11.5)