##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937904_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2223704 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1197731352734 33.0 31.0 34.0 31.0 34.0 2 32.358733896237986 34.0 31.0 34.0 31.0 34.0 3 32.46263261657127 34.0 31.0 34.0 31.0 34.0 4 36.09782866784428 37.0 35.0 37.0 35.0 37.0 5 35.91216232016492 37.0 35.0 37.0 35.0 37.0 6 36.00699733417757 37.0 35.0 37.0 35.0 37.0 7 35.96435856570839 37.0 35.0 37.0 35.0 37.0 8 35.953881451847906 37.0 35.0 37.0 35.0 37.0 9 37.62397018667952 39.0 37.0 39.0 35.0 39.0 10-11 37.55727515892403 39.0 37.0 39.0 35.0 39.0 12-13 37.467929634519706 39.0 37.0 39.0 35.0 39.0 14-15 38.695751772717955 40.0 38.0 41.0 34.0 41.0 16-17 38.6555697161133 40.0 38.0 41.0 34.0 41.0 18-19 38.61296287635405 40.0 38.0 41.0 34.0 41.0 20-21 38.504852939060235 40.0 38.0 41.0 34.0 41.0 22-23 38.35063052456621 40.0 38.0 41.0 34.0 41.0 24-25 38.29161525095067 40.0 38.0 41.0 34.0 41.0 26-27 38.20648881325932 40.0 38.0 41.0 34.0 41.0 28-29 38.097073846159375 40.0 38.0 41.0 33.0 41.0 30-31 38.192343045657154 40.0 38.0 41.0 34.0 41.0 32-33 38.13196405636722 40.0 38.0 41.0 34.0 41.0 34-35 38.00354453650306 40.0 37.0 41.0 33.0 41.0 36-37 37.83963243309361 40.0 37.0 41.0 33.0 41.0 38-39 37.68927788950328 40.0 37.0 41.0 33.0 41.0 40-41 37.50729278716952 40.0 37.0 41.0 33.0 41.0 42-43 37.29900382424999 40.0 36.5 41.0 32.0 41.0 44-45 37.035940709734746 40.0 36.0 41.0 31.0 41.0 46-47 36.728898720333284 39.0 36.0 41.0 31.0 41.0 48-49 36.42494189874192 39.0 36.0 41.0 30.0 41.0 50-51 36.17219333148657 39.0 35.0 40.0 30.0 41.0 52-53 35.9404421631656 39.0 35.0 40.0 29.0 41.0 54-55 35.710049988667556 38.5 35.0 40.0 28.5 41.0 56-57 35.40057849426003 38.0 34.5 40.0 28.0 41.0 58-59 35.11614180664333 38.0 34.0 40.0 27.5 41.0 60-61 34.73225887977897 37.5 34.0 40.0 26.5 41.0 62-63 34.33775988171087 37.0 34.0 40.0 26.0 41.0 64-65 33.916408838586435 36.5 33.0 39.0 26.0 41.0 66-67 33.475102801451996 36.0 33.0 39.0 25.0 40.0 68-69 32.99537415951044 35.0 32.0 38.5 23.5 40.0 70-71 32.568055595528904 35.0 32.0 38.0 23.0 40.0 72-73 32.102111836827206 35.0 31.0 37.0 22.0 39.0 74-75 31.601596705316894 35.0 31.0 36.5 20.5 39.0 76-77 30.404843225537213 33.5 29.5 35.5 20.0 37.5 78-79 30.97965444141846 34.0 31.0 36.0 20.0 38.0 80-81 30.77532328943061 34.0 31.0 35.0 19.5 37.0 82-83 30.411775353194493 34.0 30.0 35.0 18.0 37.0 84-85 29.999868687559136 34.0 30.0 35.0 15.0 36.0 86-87 29.564749400100013 34.0 29.5 35.0 8.5 36.0 88-89 29.092232824152855 34.0 29.0 35.0 4.5 35.5 90-91 28.539452642977665 33.0 29.0 35.0 2.0 35.0 92-93 28.04554922777492 33.0 27.5 35.0 2.0 35.0 94-95 27.51990395304411 33.0 27.0 35.0 2.0 35.0 96-97 26.905915535520915 33.0 25.5 34.5 2.0 35.0 98-99 26.01909584189263 32.0 24.5 34.0 2.0 35.0 100-101 23.55676115166407 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12263178330868385 1101 2 0.11754440549405842 1101 3 0.09478406518460503 1101 4 0.051439967156078126 1101 5 -0.07541419206149413 1101 6 0.038090019059964675 1101 7 -0.04924231707148152 1101 8 0.03003069947519066 1101 9 0.04915218125923815 1101 10-11 0.10091189976096615 1101 12-13 0.08603163978305162 1101 14-15 0.13506988222398064 1101 16-17 -0.014725174768727811 1101 18-19 0.11105357004028349 1101 20-21 0.15561800179863639 1101 22-23 0.11885295758957426 1101 24-25 0.044888252366298786 1101 26-27 0.03407157515294301 1101 28-29 1.8236180662967172E-4 1101 30-31 0.05846523942449977 1101 32-33 0.07618548579770845 1101 34-35 -0.061074408430670246 1101 36-37 0.015328528554817922 1101 38-39 0.1267529277177033 1101 40-41 0.033132674211280744 1101 42-43 -0.0956048444436135 1101 44-45 0.016500330549526154 1101 46-47 -0.1311067907579826 1101 48-49 -0.14522494223876237 1101 50-51 0.03823614862250224 1101 52-53 -0.004882582764075494 1101 54-55 0.0774632231076069 1101 56-57 0.16335434527725567 1101 58-59 0.02310471060658159 1101 60-61 0.04558543520496272 1101 62-63 0.08995105996491048 1101 64-65 0.033444861960518324 1101 66-67 0.08142948373100722 1101 68-69 0.0077694276533293305 1101 70-71 -0.07816558271476026 1101 72-73 -0.06165492639518533 1101 74-75 -0.01227535866030749 1101 76-77 -0.03347416396279357 1101 78-79 -0.014510349714338844 1101 80-81 0.019051797778381285 1101 82-83 -0.06766204426872235 1101 84-85 0.042865395813546314 1101 86-87 0.25950735052668605 1101 88-89 0.4430368150255113 1101 90-91 0.35241864065960016 1101 92-93 0.6105040388891503 1101 94-95 0.6758749847560601 1101 96-97 0.7904839394019412 1101 98-99 0.9993090648364706 1101 100-101 1.4132535891247429 1102 1 0.09340615958902987 1102 2 0.14563240820331913 1102 3 0.11505744740368584 1102 4 0.06323423368745296 1102 5 -0.0988448421117738 1102 6 -0.04836773886260204 1102 7 -0.032353674563225354 1102 8 0.00755807360338423 1102 9 0.13766529607917022 1102 10-11 0.026998581589808168 1102 12-13 0.03481030938256424 1102 14-15 0.03267568509834007 1102 16-17 -0.0019110781571427538 1102 18-19 0.10208340809835903 1102 20-21 0.16765295162989702 1102 22-23 0.1749782102252837 1102 24-25 0.008932129455089921 1102 26-27 -0.06276375338826057 1102 28-29 0.05397684521459212 1102 30-31 0.165938835223848 1102 32-33 0.14239582652022875 1102 34-35 0.14211200300879057 1102 36-37 0.08872856342773616 1102 38-39 0.16830585299429401 1102 40-41 0.27583568632353916 1102 42-43 0.274326499337036 1102 44-45 0.2718738795333522 1102 46-47 0.2501619791057479 1102 48-49 0.0680800436596698 1102 50-51 0.1908406754829457 1102 52-53 0.0865258605892194 1102 54-55 0.1246291707111169 1102 56-57 0.20179244931549079 1102 58-59 0.11051251353299563 1102 60-61 0.1787914767220471 1102 62-63 0.14302153138108764 1102 64-65 0.24894320523533509 1102 66-67 0.23433035190444684 1102 68-69 0.22334435792384255 1102 70-71 0.20141311948164287 1102 72-73 0.18088490505586918 1102 74-75 0.2085188020489035 1102 76-77 0.18146838401787946 1102 78-79 0.4028897578867934 1102 80-81 0.24975864408025217 1102 82-83 0.282268059469704 1102 84-85 0.3226807917225294 1102 86-87 0.38477417870405617 1102 88-89 0.31308810803767884 1102 90-91 0.5051561973656966 1102 92-93 0.9185460920171131 1102 94-95 0.7310927697868941 1102 96-97 0.8656293860205437 1102 98-99 1.0550282298066875 1102 100-101 1.725469600816723 1103 1 0.13333812139912737 1103 2 0.1156450556126245 1103 3 0.11024006340763037 1103 4 0.07607894156884498 1103 5 -0.1450904850488115 1103 6 -0.045527908252623206 1103 7 0.0023416913691605146 1103 8 0.014523284092412325 1103 9 0.11946193365476887 1103 10-11 0.04248670967172785 1103 12-13 0.030234187914651045 1103 14-15 0.08442487966340906 1103 16-17 0.014128978400933079 1103 18-19 0.06138375818595421 1103 20-21 -0.015943835562715947 1103 22-23 0.012554985379523487 1103 24-25 -0.03327206479531242 1103 26-27 0.03509773311879627 1103 28-29 -0.09085210517224596 1103 30-31 -0.06013550941501222 1103 32-33 0.07126049315036198 1103 34-35 0.03513519567776768 1103 36-37 -0.0038849714318160977 1103 38-39 -0.017731219343602334 1103 40-41 -0.07133696806925371 1103 42-43 -0.026693895879283502 1103 44-45 -0.011832709705991817 1103 46-47 0.0860839838723777 1103 48-49 0.10761816961000648 1103 50-51 0.1236671735310253 1103 52-53 -0.06744511888462768 1103 54-55 -0.03941196585045503 1103 56-57 -0.028596516885670553 1103 58-59 -0.013336430113959352 1103 60-61 4.618000734666339E-4 1103 62-63 0.05135039853457357 1103 64-65 0.014344801082593506 1103 66-67 0.09189975278671625 1103 68-69 -0.10239685029961265 1103 70-71 -0.006845354587113661 1103 72-73 0.1577131078650993 1103 74-75 0.0878253032842089 1103 76-77 0.08660424977669479 1103 78-79 0.16744373116279831 1103 80-81 0.24280275927927164 1103 82-83 0.1316544545723346 1103 84-85 0.14425360770983175 1103 86-87 0.17955790901987712 1103 88-89 0.4180543097141225 1103 90-91 0.49809830095670904 1103 92-93 0.6497959397609812 1103 94-95 0.5515080585355712 1103 96-97 0.6839782418664697 1103 98-99 0.9115240980828041 1103 100-101 1.6698131332807762 1104 1 0.07419036742240337 1104 2 0.16439132174519955 1104 3 0.10121388749749372 1104 4 0.044785617399341504 1104 5 -0.0429485626896593 1104 6 0.0302091311223478 1104 7 -0.008342970621200152 1104 8 0.04903709964771963 1104 9 0.15397710116997132 1104 10-11 0.12964400012777588 1104 12-13 0.07213627996198113 1104 14-15 0.08490605448213984 1104 16-17 -0.06012520677528954 1104 18-19 -0.005853447848799931 1104 20-21 0.1483873818304673 1104 22-23 0.13527760892899465 1104 24-25 0.045852187202150674 1104 26-27 -0.019870129565340733 1104 28-29 0.04864569826163745 1104 30-31 0.030044271754299245 1104 32-33 -0.009031422031114289 1104 34-35 -0.048496183360668965 1104 36-37 0.06473787059437797 1104 38-39 -0.0075156874383282 1104 40-41 0.1425300600377497 1104 42-43 0.16964620118652363 1104 44-45 0.04059223931282219 1104 46-47 0.3108522948172592 1104 48-49 0.26878383435148123 1104 50-51 0.16099785898943964 1104 52-53 0.22314176199148505 1104 54-55 0.1971708153916225 1104 56-57 0.33831304781273275 1104 58-59 0.2204230901418427 1104 60-61 0.06716338417717083 1104 62-63 0.26839969443385314 1104 64-65 0.30173988899440474 1104 66-67 0.34625054410113165 1104 68-69 0.15006236475738177 1104 70-71 0.21612134688638207 1104 72-73 0.4237120169563653 1104 74-75 0.5329371447814104 1104 76-77 0.22788952855574252 1104 78-79 0.3716489778487748 1104 80-81 0.368233850129009 1104 82-83 0.5050821866620581 1104 84-85 0.431846204672393 1104 86-87 0.5890971660023787 1104 88-89 0.5893071316231477 1104 90-91 0.40566965740019967 1104 92-93 0.6397360676023105 1104 94-95 0.7028846732673131 1104 96-97 1.0764930667212909 1104 98-99 1.2678376156169868 1104 100-101 2.06549517001082 1105 1 0.08907602612826082 1105 2 0.07844459511353818 1105 3 -0.04987964996855965 1105 4 0.014608304531442684 1105 5 -0.163242353470622 1105 6 -0.05164621636912159 1105 7 -0.06921439815776154 1105 8 -0.054399992006288755 1105 9 -0.05172957701988423 1105 10-11 -0.07919756371068587 1105 12-13 -0.15174169221628375 1105 14-15 -0.1063398909269253 1105 16-17 -0.10221028938912013 1105 18-19 -0.032071516249381204 1105 20-21 0.021829688197641417 1105 22-23 0.03783775292171043 1105 24-25 -0.0528247118457017 1105 26-27 -0.17678895091901126 1105 28-29 -0.057636201155986555 1105 30-31 -0.1128637309626015 1105 32-33 -0.14136953434028499 1105 34-35 -0.1685985239995631 1105 36-37 -0.019549852845102578 1105 38-39 -0.19078736213109693 1105 40-41 -0.12246148309078109 1105 42-43 -0.12078202368826396 1105 44-45 -0.13888349775759679 1105 46-47 0.02484136865542297 1105 48-49 -0.10868069184285645 1105 50-51 -0.07709317233492641 1105 52-53 -0.0845116751380246 1105 54-55 -0.21204919376062747 1105 56-57 -0.12126005244530091 1105 58-59 -0.04703654441934191 1105 60-61 -0.10258114535903218 1105 62-63 0.023841825631087943 1105 64-65 0.06929562723599503 1105 66-67 -0.01891744361778791 1105 68-69 -0.010295239372176468 1105 70-71 -0.0647979772605396 1105 72-73 0.1234013932024638 1105 74-75 0.2454362645146091 1105 76-77 0.07708840373252102 1105 78-79 0.22915826576782905 1105 80-81 0.35757839400188374 1105 82-83 0.22348502552565463 1105 84-85 0.3196258171631712 1105 86-87 0.2736711307518256 1105 88-89 0.49078558862334276 1105 90-91 0.4814196762675671 1105 92-93 0.7893080370803354 1105 94-95 0.9360260810934733 1105 96-97 0.8434704791382188 1105 98-99 1.1463180769752306 1105 100-101 1.4492636685495128 1106 1 -0.14643492298140615 1106 2 0.0016785879441059137 1106 3 -0.04974446589001502 1106 4 -0.03420088787914466 1106 5 -0.1283501817897772 1106 6 -0.18960844116009667 1106 7 -0.20879362737097296 1106 8 -0.1843168291098749 1106 9 -0.07829598416336836 1106 10-11 -0.09572018082106837 1106 12-13 -0.21149352200551874 1106 14-15 -0.16033465384501966 1106 16-17 -0.10181928312047717 1106 18-19 0.0019148020008898925 1106 20-21 -0.1603841038879139 1106 22-23 -0.15595889926393625 1106 24-25 -0.1855540583741373 1106 26-27 -0.2930070902469524 1106 28-29 -0.21218730060422786 1106 30-31 -0.253367363050522 1106 32-33 -0.28142811321799854 1106 34-35 -0.40215764454772795 1106 36-37 -0.24177866597693765 1106 38-39 -0.26272130582566433 1106 40-41 -0.2849701606275161 1106 42-43 -0.22179148710756635 1106 44-45 -0.08287538617909007 1106 46-47 0.04884935372405863 1106 48-49 -0.05380296203859558 1106 50-51 -0.21915509118018406 1106 52-53 -0.08324404082203785 1106 54-55 -0.08922271486949995 1106 56-57 -0.28575412102699715 1106 58-59 -0.0934012136800817 1106 60-61 0.07248016789357337 1106 62-63 -0.0726655275553938 1106 64-65 -0.23377139657642232 1106 66-67 -0.043132875527582826 1106 68-69 -0.33548447706476026 1106 70-71 -0.046564407464060764 1106 72-73 0.11286711049344689 1106 74-75 -0.016560154775426383 1106 76-77 -0.10649744669810346 1106 78-79 0.10893324633094892 1106 80-81 0.0424669758982148 1106 82-83 0.1203960226236731 1106 84-85 0.1583953164322729 1106 86-87 0.1489940445465585 1106 88-89 0.30141224489520724 1106 90-91 0.3420614389932197 1106 92-93 0.6454442198929833 1106 94-95 0.5073769183406505 1106 96-97 0.6293902766797075 1106 98-99 0.8754081827017224 1106 100-101 1.656353466275167 1107 1 -0.01304803596938342 1107 2 -0.0044237951722365665 1107 3 -0.02561258273556888 1107 4 0.02098493059883566 1107 5 -0.23630439191906305 1107 6 -0.10289601287946226 1107 7 -0.10399818114159132 1107 8 -0.06619314291422285 1107 9 0.002494868378292381 1107 10-11 -0.045598645535726234 1107 12-13 -0.0662233115500257 1107 14-15 -0.0012134313510756556 1107 16-17 -0.04242687638650011 1107 18-19 -0.02533600841317707 1107 20-21 0.043024061523475154 1107 22-23 0.05725806636979058 1107 24-25 0.011028494411419842 1107 26-27 -0.17553748949325154 1107 28-29 -0.19956284128266333 1107 30-31 -0.12162766075496734 1107 32-33 -0.15698274118938116 1107 34-35 -0.006272045184687158 1107 36-37 -0.04693678658887279 1107 38-39 -0.1793586427100422 1107 40-41 0.005316150805406039 1107 42-43 -0.05071960785899421 1107 44-45 -0.00622211195599931 1107 46-47 -0.08090850925077575 1107 48-49 -0.10122372065699636 1107 50-51 -0.019137466484792753 1107 52-53 -0.006974714015655081 1107 54-55 -0.2250836316111915 1107 56-57 0.024552315286413773 1107 58-59 0.008307094123814807 1107 60-61 0.17180894062460084 1107 62-63 -0.03548003581834536 1107 64-65 -0.010142112326832375 1107 66-67 0.08496680406243229 1107 68-69 0.04169359459505273 1107 70-71 0.12592918379966989 1107 72-73 0.13629246333609757 1107 74-75 0.12177559647654235 1107 76-77 0.11507159918619436 1107 78-79 0.07021619002527757 1107 80-81 0.020477418731427832 1107 82-83 0.12625026505148895 1107 84-85 0.25266476878442745 1107 86-87 0.3429889594061315 1107 88-89 0.43673965915656154 1107 90-91 0.40864529820597184 1107 92-93 0.5852282543332983 1107 94-95 0.6320772716929888 1107 96-97 0.8129988270981414 1107 98-99 1.2011261677242189 1107 100-101 1.789182975648714 1108 1 -0.05777411403387589 1108 2 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0.016821557772114915 2316 88-89 -0.10202223828008883 2316 90-91 -0.25462275488748176 2316 92-93 -0.2517341329188021 2316 94-95 -0.3324935171394152 2316 96-97 -0.6620110619974824 2316 98-99 -0.934844929257288 2316 100-101 -1.696038015116578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 19.0 5 38.0 6 93.0 7 227.0 8 557.0 9 1112.0 10 1773.0 11 2740.0 12 4463.0 13 6082.0 14 8051.0 15 10955.0 16 14316.0 17 18598.0 18 17143.0 19 12223.0 20 11276.0 21 11675.0 22 12411.0 23 14048.0 24 16379.0 25 19144.0 26 23634.0 27 29202.0 28 36449.0 29 45125.0 30 57004.0 31 70289.0 32 89967.0 33 118244.0 34 160810.0 35 228418.0 36 345629.0 37 485611.0 38 326768.0 39 23222.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16595990487733 19.131110385304392 14.47003662924563 23.232893080572644 2 16.02933663832956 20.046687868529265 39.7318168245414 24.192158668599777 3 20.04502397801146 23.331252720685846 29.33753772984174 27.28618557146095 4 13.343592492525982 16.135960541510922 35.924385619668804 34.59606134629429 5 13.82079996977546 37.40952315842831 34.03863253752542 14.731044334270804 6 33.66837492759828 35.67799491299202 16.299651392451516 14.353978766958193 7 29.734173253274715 32.19376319869911 19.720610297053927 18.351453250972252 8 26.7560790464918 35.65474541575677 18.71103348287362 18.87814205487781 9 26.30206178520163 16.804125009443702 19.83060695128488 37.06320625406978 10-11 25.54384486424452 26.49729910095948 26.48052528573947 21.47833074905653 12-13 26.172004470218408 23.602236008643622 27.453099808644073 22.7726597124939 14-15 22.777244633278528 25.230808596827636 25.570894327662312 26.42105244223152 16-17 22.355920572162482 28.533991034778012 26.42620150883391 22.683886884225597 18-19 22.434033486471222 26.973059364016073 28.700200206502302 21.892706943010403 20-21 22.78843306461459 25.9678405432746 29.20665350392285 22.037072888187957 22-23 22.630530788894813 25.858012739581042 29.10687797519404 22.404578496330103 24-25 22.58140965565375 26.155960447759853 29.122355973758957 22.14027392282744 26-27 22.17762870466412 26.452258016208813 29.086331066281744 22.283782212845317 28-29 22.173468513571276 26.88963655901551 28.89984635611249 22.037048571300723 30-31 22.257686186562285 26.407734506994434 28.91898041864517 22.41559888779811 32-33 22.132082327504023 26.322523141569203 29.020476646172334 22.524917884754444 34-35 22.655960505534907 26.397128394786357 28.883542953558567 22.063368146120165 36-37 22.729334194059593 26.225798538339305 28.731798101680372 22.313069165920727 38-39 22.3650494850034 26.567789597896123 28.830860582163815 22.236300334936665 40-41 22.669271629677333 26.448596575803258 28.649316635667336 22.232815158852077 42-43 22.356769870002072 26.462962992113415 28.53576983784496 22.644497300039557 44-45 22.4738769188705 26.60205674856006 28.587213046340697 22.33685328622874 46-47 22.92458888413206 26.95725240409695 27.972697805103557 22.145460906667434 48-49 22.834109215974788 26.98870892888622 27.814133535758355 22.36304831938064 50-51 22.763124048884205 27.169173595046825 27.85851893957109 22.20918341649788 52-53 23.047114869405927 27.06208359162991 27.648672586070937 22.242128952893225 54-55 22.727822587898387 27.165868299018214 27.722102402118264 22.38420671096513 56-57 22.80584556217914 27.263565654421633 27.618873734993503 22.311715048405723 58-59 22.976911495414857 26.939399308541063 27.563470677752072 22.520218518292005 60-61 22.664662203242877 27.15062346427402 27.908458140112174 22.276256192370926 62-63 22.575711515561423 27.169241050067818 28.06999043038102 22.18505700398974 64-65 22.511247000500067 27.21684180988117 27.874415839518207 22.397495350100552 66-67 22.514574781535675 27.179404273230613 27.67899864370438 22.62702230152934 68-69 22.65888355644456 27.013037706457332 27.940836550188337 22.38724218690977 70-71 22.727170522695467 27.03325172774794 27.66238222353335 22.577195526023246 72-73 22.72253656290276 27.05225340445797 27.6360996212622 22.589110411377064 74-75 22.57295601076223 27.460726544866827 27.38992122599106 22.576396218379884 76-77 22.774074247291907 27.107407280825147 27.592318941729655 22.526199530153292 78-79 22.769352395822466 27.24353151318701 27.493047635836426 22.494068455154103 80-81 22.864149185323228 27.374214373855516 27.3465128452348 22.415123595586465 82-83 22.791575845853377 27.471229270481956 27.37335190297616 22.3638429806885 84-85 22.676562790197774 27.537539927840953 27.294431485556274 22.491465796405 86-87 22.763731144073134 27.530080442361033 27.192985217457 22.513203196108837 88-89 22.708485481880682 27.528214186780254 27.000738407629793 22.76256192370927 90-91 22.810276549187474 27.36713291509418 27.193242632149754 22.629347903568583 92-93 22.807522091060726 27.15085690695019 27.392465586784176 22.64915541520491 94-95 22.70124530962754 27.105001385076434 27.494396736256267 22.699356569039765 96-97 22.78012271417419 26.94940513665488 27.42233228882981 22.848139860341124 98-99 22.51517010961693 27.247263961368862 27.168116540978914 23.06944938803529 100-101 22.504599893587404 26.894389321680418 27.61280927003928 22.9882015146929 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 336.0 1 254.5 2 223.5 3 287.0 4 234.5 5 225.0 6 455.5 7 955.5 8 1557.0 9 1917.5 10 1842.5 11 1643.0 12 1786.5 13 2022.0 14 2194.5 15 2352.0 16 2528.0 17 2762.5 18 2928.5 19 3191.0 20 3615.0 21 4208.0 22 5031.0 23 6220.5 24 7824.0 25 9895.5 26 12742.5 27 15800.5 28 17907.0 29 20679.5 30 24627.5 31 28870.0 32 34844.5 33 38874.5 34 41333.5 35 45152.5 36 50287.0 37 55850.0 38 59054.0 39 61452.5 40 64030.0 41 67039.5 42 69125.5 43 71317.5 44 75327.0 45 78884.5 46 80599.5 47 84391.0 48 88499.0 49 86634.5 50 82302.5 51 78822.0 52 76811.5 53 76351.0 54 76983.5 55 73267.5 56 66209.0 57 60921.5 58 57203.5 59 50828.5 60 41236.0 61 33871.0 62 28225.0 63 21627.0 64 15292.0 65 11223.0 66 8359.5 67 6558.0 68 5018.5 69 3660.5 70 2644.5 71 1759.5 72 1305.5 73 1085.5 74 813.5 75 565.0 76 406.0 77 241.0 78 157.0 79 119.5 80 74.5 81 45.0 82 29.5 83 17.5 84 8.5 85 5.0 86 4.0 87 2.5 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04002331245525483 2 0.0 3 0.0 4 0.0 5 0.015514654828160583 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004452031385472168 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.5739504898133923E-4 22-23 2.0236506297600758E-4 24-25 5.396401679360203E-4 26-27 0.0016863755248000632 28-29 0.002495835776704094 30-31 3.1479009796267846E-4 32-33 0.0 34-35 0.0 36-37 2.248500699733418E-5 38-39 0.0 40-41 0.0 42-43 0.011040138435691082 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.248500699733418E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.994002798933672E-5 74-75 4.497001399466836E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3491004198400508E-4 84-85 2.2485006997334178E-4 86-87 0.0 88-89 0.0 90-91 0.007892237456064296 92-93 0.0030129909376427795 94-95 0.0 96-97 0.0 98-99 2.4733507697067593E-4 100-101 0.012726513960491145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2223704.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.23462714043893 #Duplication Level Percentage of deduplicated Percentage of total 1 76.44302105217773 42.223017653057596 2 13.324114758429797 14.719050213165785 3 4.359281734037859 7.223499035391217 4 1.955097747458753 4.319563804159849 5 1.060588069300703 2.929059327871116 6 0.6219735013448157 2.0612684662808505 7 0.4116005646111144 1.591422260196233 8 0.29304852435648104 1.2949140781508852 9 0.20205512908471557 1.0044395745126768 >10 1.1941033879098653 12.29427038036765 >50 0.08888450164895875 3.3205347085862242 >100 0.04175820910707284 4.077079545832829 >500 0.003090312367634705 1.1604377952606346 >1k 0.0012198601451188706 1.17409317537047 >5k 1.6264801934918273E-4 0.6073499817960105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7218 0.32459356101351616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6303 0.28344599820839467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3989 0.17938538582473207 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2834 0.1274450196608901 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2255 0.10140738155797714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.497001399466836E-5 0.0 0.0 0.0 0.0 8 4.497001399466836E-5 0.0 0.0 0.0 0.0 9 4.497001399466836E-5 0.0 0.0 0.0 0.0 10-11 4.497001399466836E-5 0.0 0.0 8.99400279893367E-5 0.0 12-13 6.745502099200254E-5 0.0 0.0 1.3491004198400505E-4 0.0 14-15 1.7988005597867343E-4 0.0 0.0 1.3491004198400505E-4 0.0 16-17 1.7988005597867343E-4 0.0 0.0 2.2485006997334178E-4 0.0 18-19 2.023650629760076E-4 0.0 0.0 2.6982008396801016E-4 0.0 20-21 2.2485006997334178E-4 0.0 0.0 3.147900979626785E-4 0.0 22-23 2.2485006997334178E-4 0.0 0.0 4.047301259520152E-4 1.3491004198400505E-4 24-25 2.2485006997334178E-4 0.0 0.0 5.846101819306887E-4 1.3491004198400505E-4 26-27 2.2485006997334178E-4 0.0 0.0 7.195202239146937E-4 1.3491004198400505E-4 28-29 4.047301259520152E-4 0.0 0.0 0.0010343103218773723 1.3491004198400505E-4 30-31 4.4970013994668356E-4 0.0 0.0 0.0022709857067307517 1.3491004198400505E-4 32-33 4.4970013994668356E-4 0.0 0.0 0.003417721063594795 1.3491004198400505E-4 34-35 5.17155160938686E-4 0.0 0.0 0.004586941427456172 1.3491004198400505E-4 36-37 5.846101819306887E-4 0.0 0.0 0.006543137036224246 1.3491004198400505E-4 38-39 6.29580195925357E-4 4.497001399466836E-5 0.0 0.010163223162795047 1.3491004198400505E-4 40-41 6.29580195925357E-4 4.497001399466836E-5 0.0 0.013625914240384512 1.3491004198400505E-4 42-43 6.970352169173595E-4 4.497001399466836E-5 0.0 0.01632411508006461 1.3491004198400505E-4 44-45 8.094602519040304E-4 4.497001399466836E-5 0.0 0.018595100786795365 1.3491004198400505E-4 46-47 8.994002798933671E-4 4.497001399466836E-5 0.0 0.022305126941355505 1.3491004198400505E-4 48-49 8.994002798933671E-4 4.497001399466836E-5 0.0 0.025587937962966295 1.3491004198400505E-4 50-51 8.994002798933671E-4 4.497001399466836E-5 0.0 0.028825778970582416 1.3491004198400505E-4 52-53 8.994002798933671E-4 4.497001399466836E-5 0.0 0.03721268658058806 1.3491004198400505E-4 54-55 9.443702938880355E-4 4.497001399466836E-5 0.0 0.05214273122681796 1.3491004198400505E-4 56-57 9.443702938880355E-4 4.497001399466836E-5 0.0 0.0728963926853574 1.3491004198400505E-4 58-59 9.443702938880355E-4 4.497001399466836E-5 0.0 0.1036109122437159 1.3491004198400505E-4 60-61 9.443702938880355E-4 8.994002798933672E-5 0.0 0.141430694013232 1.3491004198400505E-4 62-63 9.443702938880355E-4 8.994002798933672E-5 0.0 0.16521983141641156 1.3491004198400505E-4 64-65 9.443702938880355E-4 1.3491004198400508E-4 0.0 0.19699114630364473 1.3491004198400505E-4 66-67 9.893403078827037E-4 1.7988005597867343E-4 0.0 0.2538107589859082 1.3491004198400505E-4 68-69 0.0010792803358720404 1.7988005597867343E-4 0.0 0.33014735774185777 1.3491004198400505E-4 70-71 0.0010792803358720404 1.7988005597867343E-4 0.0 0.4070235966657433 1.3491004198400505E-4 72-73 0.0011017653428693746 1.7988005597867343E-4 0.0 0.4932086284865252 1.3491004198400505E-4 74-75 0.0011242503498667088 1.7988005597867343E-4 0.0 0.5787415951043844 1.3491004198400505E-4 76-77 0.0011242503498667088 1.7988005597867343E-4 0.0 0.6679171328558118 1.3491004198400505E-4 78-79 0.0011242503498667088 1.7988005597867343E-4 0.0 0.7522808791098097 1.3491004198400505E-4 80-81 0.0012141903778560457 1.7988005597867343E-4 0.0 0.8419061170011837 1.3491004198400505E-4 82-83 0.0012816453988480482 1.7988005597867343E-4 0.0 0.958603303317348 1.3491004198400505E-4 84-85 0.0013491004198400507 1.7988005597867343E-4 0.0 1.1285449862031998 1.3491004198400505E-4 86-87 0.001394070433834719 1.7988005597867343E-4 0.0 1.3204095509114522 1.3491004198400505E-4 88-89 0.0014615254548267216 1.7988005597867343E-4 0.0 1.5572890996283677 1.3491004198400505E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7575 0.0 29.928509 1 GTATCAA 10900 0.0 28.778408 1 GTACATG 7820 0.0 26.255865 1 TACATGG 7695 0.0 25.621422 2 TGCACCG 450 0.0 24.281698 5 ATCAACG 12915 0.0 24.167664 3 TCAACGC 12910 0.0 24.103428 4 CAACGCA 13130 0.0 23.771925 5 TATCAAC 13160 0.0 23.717735 2 ACATGGG 7940 0.0 23.694002 3 TAAGGTG 1045 0.0 23.64018 5 AACGCAG 13425 0.0 23.351 6 CATGGGG 3965 0.0 22.166252 4 GAGTACT 7975 0.0 21.621262 12-13 GTAAGGT 1015 0.0 21.53057 4 ACGCAGA 14885 0.0 21.030117 7 AGAGTAC 11620 0.0 21.011747 10-11 CGCAGAG 14960 0.0 20.892933 8 GTACTTT 8405 0.0 20.3442 14-15 AGTACTT 8370 0.0 20.033388 12-13 >>END_MODULE