FastQCFastQC Report
Thu 26 May 2016
SRR937903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180967
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117620.539302061883559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92470.42398624096559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63640.29179717070455446No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30350.13915845585925876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.11348177207633127No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24540.11251889643447151No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23380.10720015479372225No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22350.10247747902650522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.057.177431
GTATCAA98850.046.863561
TGGTATC20300.035.3642232
TATCAAC133050.034.6609152
ATCAACG133550.034.5651553
TCAACGC135450.034.1177524
CAACGCA137500.033.471555
AACGCAG141550.032.6532756
GTGGTAT23850.031.1092281
CGCAGAG163000.028.0059038
TACATGG119400.027.9521982
ACGCAGA164050.027.9135177
GTACATG122950.027.7359891
ACATGGG121550.026.0484853
GCAGAGT176900.025.214579
GGACCGT2602.3646862E-1123.751656
ACCGTAT1201.7109615E-423.7511048
GAGTACT105800.023.7067512-13
CATGGGA64900.023.5887184
GTACTTT109500.022.7316514-15
Produced by FastQC (version 0.11.5)