##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937903_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180967 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40291118572633 31.0 31.0 34.0 30.0 34.0 2 31.525029493797934 33.0 31.0 34.0 30.0 34.0 3 31.535296499213423 33.0 31.0 34.0 28.0 34.0 4 35.1595984716871 37.0 35.0 37.0 33.0 37.0 5 35.143608775373494 37.0 35.0 37.0 33.0 37.0 6 35.20884635118276 37.0 35.0 37.0 32.0 37.0 7 35.134117114105806 37.0 35.0 37.0 32.0 37.0 8 35.11639515866127 37.0 35.0 37.0 32.0 37.0 9 36.69517420483666 39.0 37.0 39.0 32.0 39.0 10-11 36.54784666618065 39.0 37.0 39.0 32.0 39.0 12-13 36.401697962417586 39.0 37.0 39.0 32.0 39.0 14-15 37.45535397830412 40.0 37.0 41.0 32.0 41.0 16-17 37.351083028766595 40.0 37.0 41.0 32.0 41.0 18-19 37.297113390528146 40.0 37.0 41.0 32.0 41.0 20-21 37.1912690563406 40.0 37.0 41.0 31.0 41.0 22-23 37.101209004996406 39.0 36.5 41.0 31.0 41.0 24-25 37.11502076831057 40.0 37.0 41.0 31.0 41.0 26-27 37.00679308765332 39.0 36.0 41.0 31.0 41.0 28-29 36.85523508608796 39.0 36.0 41.0 30.5 41.0 30-31 36.564103445856816 39.0 36.0 41.0 30.0 41.0 32-33 36.365121984881014 39.0 35.0 40.5 30.0 41.0 34-35 36.31869785283317 39.0 35.0 41.0 30.0 41.0 36-37 36.1372310997828 39.0 35.0 41.0 29.5 41.0 38-39 35.94246290750846 39.0 35.0 41.0 29.0 41.0 40-41 35.66489153664406 39.0 35.0 40.5 27.5 41.0 42-43 35.333321641271965 38.0 35.0 40.0 26.0 41.0 44-45 35.039784875241125 38.0 34.5 40.0 25.5 41.0 46-47 34.609645171155734 38.0 34.0 40.0 23.5 41.0 48-49 34.21532260689868 38.0 33.5 40.0 22.5 41.0 50-51 33.272597201149765 37.5 32.0 39.5 19.5 40.5 52-53 33.32455603408947 37.5 33.0 39.5 20.0 40.5 54-55 33.56653195577925 38.0 33.0 40.0 18.5 41.0 56-57 33.281128508592744 38.0 33.0 40.0 17.0 41.0 58-59 32.99131692501537 37.0 32.5 40.0 13.5 41.0 60-61 32.624191012518764 37.0 32.0 40.0 10.5 41.0 62-63 32.211101314233545 36.0 31.0 39.0 8.0 41.0 64-65 31.78060442913625 35.5 31.0 39.0 7.0 40.5 66-67 31.313958899882486 35.0 30.5 39.0 2.0 40.0 68-69 30.771900033333836 35.0 30.0 38.0 2.0 40.0 70-71 30.28222710384889 35.0 29.0 37.5 2.0 40.0 72-73 29.81531013536656 34.0 29.0 37.0 2.0 39.0 74-75 29.269427047726992 34.0 28.5 36.0 2.0 39.0 76-77 28.77495028581359 34.0 27.5 36.0 2.0 38.5 78-79 28.201593834294606 34.0 26.5 35.0 2.0 37.5 80-81 27.63985264334582 33.0 26.0 35.0 2.0 37.0 82-83 27.11387861439444 33.0 25.5 35.0 2.0 36.0 84-85 26.588847745059873 32.5 24.5 35.0 2.0 36.0 86-87 26.036516141693113 32.0 23.5 35.0 2.0 35.5 88-89 25.524172763732786 32.0 20.5 34.5 2.0 35.0 90-91 24.9281843787641 31.5 18.5 34.0 2.0 35.0 92-93 24.249922396808387 31.0 12.0 34.0 2.0 35.0 94-95 23.475482205828882 31.0 4.0 34.0 2.0 35.0 96-97 22.592113498278515 30.5 2.0 34.0 2.0 35.0 98-99 21.492703695195754 29.5 2.0 34.0 2.0 35.0 100-101 18.753334644678255 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15121518930547495 1101 2 0.3663774490587528 1101 3 0.2849695791381208 1101 4 0.13173827118643544 1101 5 0.07938266787162007 1101 6 0.182941664141147 1101 7 0.2563532826644206 1101 8 0.25991119618160496 1101 9 0.23885579718615446 1101 10-11 0.22695092414750206 1101 12-13 0.23174866247962456 1101 14-15 0.43735993302073695 1101 16-17 0.3932819625031314 1101 18-19 0.4957330303099283 1101 20-21 0.4682205353958224 1101 22-23 0.4120657134793433 1101 24-25 0.4658562877625414 1101 26-27 0.42482267726958867 1101 28-29 0.43658200842067885 1101 30-31 0.4241490249244322 1101 32-33 0.469140609354163 1101 34-35 0.41359542465573185 1101 36-37 0.572206685954832 1101 38-39 0.5058560888590691 1101 40-41 0.5281152700031129 1101 42-43 0.4143865229992585 1101 44-45 0.6423753963206593 1101 46-47 0.5589973903809593 1101 48-49 0.719819454622673 1101 50-51 0.68098273386682 1101 52-53 0.6198091290875638 1101 54-55 0.712562875693358 1101 56-57 0.43924578564772077 1101 58-59 0.41273158088888806 1101 60-61 0.622820983847916 1101 62-63 0.5214368500676869 1101 64-65 0.5280522097457307 1101 66-67 0.5861434332007356 1101 68-69 0.4873820952967307 1101 70-71 0.6036454129048003 1101 72-73 0.6047914548959277 1101 74-75 0.5050378028340852 1101 76-77 0.478534058113663 1101 78-79 0.5559107347268011 1101 80-81 0.7958637429487183 1101 82-83 0.5860550693312163 1101 84-85 0.6642337306914001 1101 86-87 0.8106523836212602 1101 88-89 0.787952645211341 1101 90-91 0.9742341689622549 1101 92-93 0.7980883752005212 1101 94-95 0.8921297444148486 1101 96-97 1.2156197521047183 1101 98-99 1.2342351242583014 1101 100-101 1.206549846492436 1102 1 0.20159338589715148 1102 2 0.21933085135821173 1102 3 0.3172737121081006 1102 4 0.20511752314209275 1102 5 0.23023269153646453 1102 6 0.17044309726076534 1102 7 0.26181354189004225 1102 8 0.1987118440986535 1102 9 0.2837061207037195 1102 10-11 0.2496718230001207 1102 12-13 0.294558400049695 1102 14-15 0.37669061471531506 1102 16-17 0.4826183766761787 1102 18-19 0.4252167838780281 1102 20-21 0.4653540021930169 1102 22-23 0.4538704740822297 1102 24-25 0.5319358898403408 1102 26-27 0.3028625811991361 1102 28-29 0.4285882876893865 1102 30-31 0.4372822800608773 1102 32-33 0.34215239474193737 1102 34-35 0.538965022529716 1102 36-37 0.6549533956591844 1102 38-39 0.7512914191527145 1102 40-41 0.6518633114063022 1102 42-43 0.7324379506803709 1102 44-45 0.7298921160495055 1102 46-47 0.762981903869921 1102 48-49 0.8795460007015166 1102 50-51 0.8368732229501177 1102 52-53 0.7508191917920541 1102 54-55 0.8217712939114747 1102 56-57 0.8833282510250271 1102 58-59 0.9241589092305631 1102 60-61 0.862798700675242 1102 62-63 0.781914203838042 1102 64-65 0.868893913967483 1102 66-67 0.9932387716668423 1102 68-69 0.9883319228814145 1102 70-71 0.8412854544138249 1102 72-73 1.0712082442964288 1102 74-75 1.0083993869971586 1102 76-77 0.9489609366762544 1102 78-79 1.0293593643704781 1102 80-81 1.0778740496510117 1102 82-83 1.1391363499963028 1102 84-85 1.1382891211319794 1102 86-87 1.1339162510746021 1102 88-89 1.2199060680889247 1102 90-91 1.2903383178067926 1102 92-93 1.283103088561898 1102 94-95 1.3415795734693994 1102 96-97 1.7571931557564575 1102 98-99 1.823907255887768 1102 100-101 1.9040263800774113 1103 1 0.20018455882695463 1103 2 0.17588035452214967 1103 3 0.1291917169400989 1103 4 0.13415550417224154 1103 5 0.25458000019190763 1103 6 0.1733318263687167 1103 7 0.09580004866558056 1103 8 0.20273336493767147 1103 9 0.08229564072730255 1103 10-11 0.1862955416534433 1103 12-13 0.32021122913644007 1103 14-15 0.28818795491371674 1103 16-17 0.4264253502875306 1103 18-19 0.3661620258497251 1103 20-21 0.33530086125797 1103 22-23 0.4310912224630741 1103 24-25 0.4183192483804916 1103 26-27 0.3657622745850304 1103 28-29 0.17116054035263062 1103 30-31 0.34784297339994197 1103 32-33 0.3002804233482266 1103 34-35 0.3732636530969131 1103 36-37 0.37054360714852663 1103 38-39 0.3544526023029917 1103 40-41 0.34467289385464284 1103 42-43 0.2609161474698709 1103 44-45 0.283649860603866 1103 46-47 0.2083902681263794 1103 48-49 0.27536938216196916 1103 50-51 0.08923481271532552 1103 52-53 0.11451035177409352 1103 54-55 0.2827709715947577 1103 56-57 0.20963397806959705 1103 58-59 0.258445143001083 1103 60-61 0.3581578909715759 1103 62-63 0.4200608000909014 1103 64-65 0.3845532720337985 1103 66-67 0.33210603503265546 1103 68-69 0.47536487356696 1103 70-71 0.37832074671651483 1103 72-73 0.2906792041115196 1103 74-75 0.29216371562797505 1103 76-77 0.46674758505075786 1103 78-79 0.5183887502882669 1103 80-81 0.5593196263362223 1103 82-83 0.5807675480502787 1103 84-85 0.6484429240559706 1103 86-87 0.6750017145908274 1103 88-89 0.7709841761724654 1103 90-91 0.8627375387369938 1103 92-93 0.8628795054468874 1103 94-95 0.7003241360966435 1103 96-97 0.9160024572107339 1103 98-99 1.0801752140251608 1103 100-101 1.0803429887546194 1104 1 0.23027223675395092 1104 2 0.22390850139387553 1104 3 0.16300730961144083 1104 4 0.11546762547218492 1104 5 0.09002513934976974 1104 6 0.04859238781308761 1104 7 0.10357082661285943 1104 8 0.060033784137054624 1104 9 0.1411370765620461 1104 10-11 0.10504507507126704 1104 12-13 0.21048752482965938 1104 14-15 0.2469428809236689 1104 16-17 0.2940392336652593 1104 18-19 0.19198472550078804 1104 20-21 0.3587496786203559 1104 22-23 0.32802473461264725 1104 24-25 0.2220240645624827 1104 26-27 0.31308806707211545 1104 28-29 0.43192179167991895 1104 30-31 0.430026779990321 1104 32-33 0.5089836630313798 1104 34-35 0.41332709949024604 1104 36-37 0.3984044255753858 1104 38-39 0.4658650486515299 1104 40-41 0.5711133702646904 1104 42-43 0.40811063824317984 1104 44-45 0.28989060031994995 1104 46-47 0.22742611832472903 1104 48-49 0.23622487211057575 1104 50-51 0.42997461663224357 1104 52-53 0.3443813599165537 1104 54-55 0.4427425682675121 1104 56-57 0.23941092324520952 1104 58-59 0.28151270234375403 1104 60-61 0.3975740506555425 1104 62-63 0.3531451432178514 1104 64-65 0.39847467895582156 1104 66-67 0.3592599320587375 1104 68-69 0.19263978773779655 1104 70-71 0.23683994443230816 1104 72-73 0.2726284241617769 1104 74-75 0.4044453881044099 1104 76-77 0.25567459095889333 1104 78-79 0.31863809180358516 1104 80-81 0.44840955176211494 1104 82-83 0.5144873212101011 1104 84-85 0.618576746411339 1104 86-87 0.5877587080538476 1104 88-89 0.6472141746179219 1104 90-91 0.5894170808760606 1104 92-93 0.6640626261065741 1104 94-95 0.47338831409082616 1104 96-97 0.49126767349847356 1104 98-99 0.6047286345006775 1104 100-101 0.7050095307066151 1105 1 0.05659311146192181 1105 2 0.12114276959588466 1105 3 0.11463719672520156 1105 4 0.057854503342937846 1105 5 0.06392279066677276 1105 6 0.20713132054557093 1105 7 0.11715227567817976 1105 8 0.10085738947678635 1105 9 0.23952473946583552 1105 10-11 0.21079242275409626 1105 12-13 0.22881125283080905 1105 14-15 0.20940754500123404 1105 16-17 0.26143299944612153 1105 18-19 0.2742518480904934 1105 20-21 0.2809238427300045 1105 22-23 0.3729169246661286 1105 24-25 0.2585677301384166 1105 26-27 0.1909465497693077 1105 28-29 0.19806780782593592 1105 30-31 0.3191465239487741 1105 32-33 0.2696608672569738 1105 34-35 0.3181843027345437 1105 36-37 0.28541156258253153 1105 38-39 0.3194743135511189 1105 40-41 0.45293759079858376 1105 42-43 0.39392909180357805 1105 44-45 0.30547803526222594 1105 46-47 0.24954480173374094 1105 48-49 0.4116382410723318 1105 50-51 0.2570720324393321 1105 52-53 0.341275195520069 1105 54-55 0.2340558757098492 1105 56-57 0.3129143596518631 1105 58-59 0.32725125614682327 1105 60-61 0.4056442280805541 1105 62-63 0.39713341301257543 1105 64-65 0.43914637285331537 1105 66-67 0.5601019554814037 1105 68-69 0.4487898422749481 1105 70-71 0.3328177823456251 1105 72-73 0.3527244397416638 1105 74-75 0.5265887925058799 1105 76-77 0.4489587842430858 1105 78-79 0.5119647448076563 1105 80-81 0.4702914042891315 1105 82-83 0.46180330078414755 1105 84-85 0.4568863589144989 1105 86-87 0.5156658778965024 1105 88-89 0.47409174343097504 1105 90-91 0.6785221054649568 1105 92-93 0.6912321290825325 1105 94-95 0.74799796773274 1105 96-97 0.9303370319226687 1105 98-99 1.0089659258347403 1105 100-101 1.123546312146619 1106 1 0.18563736401554465 1106 2 0.30156223195742626 1106 3 0.10437073543659281 1106 4 0.24158624499631287 1106 5 0.18203331252516364 1106 6 0.16691412487692503 1106 7 0.31230189331502345 1106 8 0.3899318906466931 1106 9 0.3653414730338511 1106 10-11 0.11292670819332784 1106 12-13 -0.07071980990298243 1106 14-15 0.06112741184035286 1106 16-17 0.12333962498136941 1106 18-19 0.16996632515079568 1106 20-21 0.04548482620069905 1106 22-23 0.18422775152210846 1106 24-25 0.25394410775499665 1106 26-27 0.23589288219434223 1106 28-29 0.37502064342442765 1106 30-31 0.14311733152264594 1106 32-33 0.23129374839582084 1106 34-35 0.47540089287277 1106 36-37 0.45931285275297995 1106 38-39 0.5596621744800387 1106 40-41 0.48830653183192396 1106 42-43 0.5768083470848708 1106 44-45 0.5746047390141413 1106 46-47 0.5513997967318289 1106 48-49 0.5101870957966597 1106 50-51 0.41870697154445935 1106 52-53 0.40848872312541573 1106 54-55 0.37284093613034486 1106 56-57 0.35765995316236854 1106 58-59 0.3481376954744917 1106 60-61 0.2901860410721113 1106 62-63 0.30281716707792583 1106 64-65 0.42713744197726555 1106 66-67 0.410001045371974 1106 68-69 0.23228498756736826 1106 70-71 -0.009468227529819018 1106 72-73 0.3252249524891937 1106 74-75 0.3307912642977904 1106 76-77 0.5643318621374611 1106 78-79 0.5167444587015133 1106 80-81 0.3917231349201806 1106 82-83 0.29133044291776145 1106 84-85 0.4141479801454544 1106 86-87 0.6034972627892152 1106 88-89 0.7167343395659067 1106 90-91 0.714183347472023 1106 92-93 0.7078130587810101 1106 94-95 0.7091071138929905 1106 96-97 1.0012135651075518 1106 98-99 1.1436263334934473 1106 100-101 1.3238642156161404 1107 1 0.06601162088043111 1107 2 0.13997978843290326 1107 3 0.10399018607818888 1107 4 0.1938086392970746 1107 5 0.13558536232934415 1107 6 0.08059569400994349 1107 7 0.1490933076192107 1107 8 0.18316697178637042 1107 9 0.07244857238966773 1107 10-11 0.14138210334377987 1107 12-13 0.259933283952833 1107 14-15 0.17050500609869346 1107 16-17 0.21188344989656827 1107 18-19 0.3037358775745176 1107 20-21 0.22031778212394215 1107 22-23 0.2076835094327194 1107 24-25 0.12466109623177601 1107 26-27 0.1856230444458049 1107 28-29 0.15056576032389302 1107 30-31 0.2548549596572087 1107 32-33 0.2033218009179123 1107 34-35 0.2829672675175132 1107 36-37 0.21374899091996014 1107 38-39 0.040399784476591094 1107 40-41 0.04712268498367678 1107 42-43 0.04953924741372617 1107 44-45 0.14966312944731897 1107 46-47 0.12771842990736815 1107 48-49 0.06704364647773531 1107 50-51 -0.041249015881710704 1107 52-53 -0.031370177125303655 1107 54-55 0.006987898641874324 1107 56-57 -0.13446483772732876 1107 58-59 -0.1457217168261451 1107 60-61 -0.10397903154269983 1107 62-63 -0.13951687363771015 1107 64-65 -0.13456370085675928 1107 66-67 -0.022207626828180338 1107 68-69 0.06426895775406294 1107 70-71 -0.007681808153961356 1107 72-73 0.08839192540914809 1107 74-75 -0.13106886515177507 1107 76-77 0.05338138866569153 1107 78-79 0.2259287087716224 1107 80-81 0.3462127802105073 1107 82-83 0.4349810239618712 1107 84-85 0.16347931550745542 1107 86-87 0.1925700548006759 1107 88-89 0.19767896701819865 1107 90-91 0.40395208089493195 1107 92-93 0.36260805045845146 1107 94-95 0.6216670914018643 1107 96-97 0.8433181949038335 1107 98-99 0.9547071215759395 1107 100-101 0.894226082826389 1108 1 -0.002307956664239441 1108 2 -0.1720630494401938 1108 3 0.0719474086479579 1108 4 -0.020586231924369258 1108 5 -0.019875197190252436 1108 6 0.09365487865584754 1108 7 0.1990468169823174 1108 8 0.13391611072739096 1108 9 0.14308764487375925 1108 10-11 0.13012033839197557 1108 12-13 0.09856997498805242 1108 14-15 0.032466850532863134 1108 16-17 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-0.4885860902555166 2316 70-71 -0.5922959832605414 2316 72-73 -0.4098150891676937 2316 74-75 -0.5540342015534669 2316 76-77 -0.6792270710250072 2316 78-79 -0.6790719154537257 2316 80-81 -0.5940701976575937 2316 82-83 -0.6868099536436709 2316 84-85 -0.8538332849638657 2316 86-87 -0.7829472263740129 2316 88-89 -0.9956577813211709 2316 90-91 -0.7917978025186514 2316 92-93 -0.8623368096357034 2316 94-95 -0.8187423403642988 2316 96-97 -0.7000902428210019 2316 98-99 -0.9056527025083518 2316 100-101 -0.8155765933199213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10045.0 3 2568.0 4 2360.0 5 2682.0 6 3099.0 7 3822.0 8 4708.0 9 5794.0 10 7463.0 11 9477.0 12 12055.0 13 14589.0 14 16903.0 15 20198.0 16 24513.0 17 30164.0 18 26612.0 19 20606.0 20 20374.0 21 21118.0 22 22776.0 23 24984.0 24 27868.0 25 31265.0 26 35416.0 27 41380.0 28 48258.0 29 56948.0 30 67725.0 31 81977.0 32 100419.0 33 126575.0 34 165558.0 35 222181.0 36 296275.0 37 346524.0 38 207876.0 39 17812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35050980056563 18.399933379250122 14.534963701353165 22.71459311883108 2 15.498604193398545 19.74553548844033 40.64156514300694 24.114295175154183 3 19.44781831067337 24.522062019147413 29.200425130139767 26.82969454003945 4 12.657599692412127 15.948210443822441 36.25655256910379 35.13763729466164 5 13.494639235004346 37.72391715306813 33.47840285539503 15.303040756532498 6 32.51573910622277 35.452710085249855 17.01155931789089 15.019991490636484 7 29.05037994818241 33.08427686710796 19.768700025407988 18.096643159301642 8 27.183504726443363 34.36418838141254 19.05624591656796 19.396060975576138 9 27.08930235293469 16.493515324491067 19.938975867854865 36.47820645471938 10-11 25.273919147701413 26.781758159908453 26.12574445975822 21.818578232631914 12-13 26.344990831774666 23.992241811426307 27.283499170012576 22.37926818678645 14-15 23.2397881651169 25.091494098455485 26.076857569687938 25.591860166739682 16-17 22.063606860785097 28.52763019506343 26.817143600189127 22.591619343962353 18-19 22.069663083445093 26.44428088156715 29.848594071397034 21.63746196359072 20-21 22.699724859615923 25.379322986511276 30.3703713912805 21.5505807625923 22-23 22.482394928324382 25.291580231049842 30.296503931494982 21.929520909130794 24-25 22.06403462224948 25.63112327055294 30.424830070269017 21.880012036928562 26-27 21.46027166616324 26.039800115079686 30.203870214918783 22.296058003838287 28-29 21.765132789757725 26.426104862107756 30.05511646227253 21.75364588586199 30-31 21.77963048736555 25.918474345118625 30.11783453538896 22.18406063212687 32-33 21.517941516576766 25.87592680623107 30.139551383394213 22.46658029379795 34-35 22.23525416812941 26.06303152725114 29.966810275902507 21.734904028716944 36-37 22.282532674563118 25.68266713277622 29.76935616014538 22.265444032515276 38-39 21.82382204947097 26.093880516082624 29.773094793906168 22.309202640540235 40-41 22.242043764034996 25.997801026172475 29.71414488651306 22.04601032327947 42-43 21.804215594391458 25.934289835896873 29.63637282466876 22.625121745042907 44-45 21.936333094041636 26.27762814070352 29.662391959798995 22.123646805455852 46-47 22.386397077657964 26.642433275663592 28.731707507580495 22.23946213909795 48-49 22.271284171835514 26.684608316103837 28.529290144727774 22.514817367332874 50-51 22.59517425534354 27.096850081292896 28.22100547719055 22.086970186173016 52-53 22.619922502103822 26.825178405561907 28.253112302090127 22.301786790244144 54-55 22.299086510455236 27.013912752652168 28.343879840566583 22.34312089632601 56-57 22.378462820341454 27.377610765925724 28.011093702559975 22.232832711172847 58-59 22.3501692951063 26.72118222482914 28.278885525366015 22.649762954698545 60-61 22.147342604106743 27.07040574847659 28.507570825424928 22.274680821991737 62-63 22.101814822887057 27.03833701675096 28.765704093910273 22.09414406645172 64-65 22.014153118609876 26.94618011857497 28.655058727128758 22.384608035686398 66-67 21.92036744653366 27.15609013922779 28.30628965121286 22.617252763025693 68-69 22.278294014940943 26.84633832972511 28.627988154183036 22.24737950115091 70-71 22.278191287428594 26.962597644430392 28.247750270945854 22.511460797195163 72-73 22.078643841102917 27.2526786600086 28.03654956255902 22.632127936329464 74-75 22.078899506926884 27.669511251524703 27.81801424443648 22.433574997111933 76-77 22.30017758725708 27.227971325124322 27.95154982319365 22.520301264424944 78-79 22.33270323097807 27.31590177753176 27.960236942985304 22.391158048504867 80-81 22.38464352735316 27.58158430399132 27.59120724766127 22.442564920994254 82-83 22.434384368453422 27.518903980227698 27.559300142798943 22.48741150851994 84-85 22.403656435090607 27.659058508840257 27.55222953807263 22.385055517996502 86-87 22.43286643738652 27.663429847002917 27.392165269086515 22.511538446524046 88-89 22.432382033701508 27.593522417454825 27.306828119373588 22.66726742947008 90-91 22.45329880314116 27.517257440405103 27.398357029203062 22.63108672725068 92-93 22.358791985672003 27.113197204487903 27.692407322821573 22.835603487018524 94-95 22.31522388416187 27.142126499357072 27.689082220884586 22.853567395596475 96-97 22.4401130633763 27.002286437630964 27.558577907445663 22.999022591547067 98-99 22.218379115301477 27.1083498923777 27.552205774949496 23.121065217371335 100-101 22.629358394303882 26.430981494726474 28.035667339036035 22.903992771933613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3271.0 1 1927.5 2 669.5 3 755.5 4 662.0 5 628.0 6 1022.5 7 2021.5 8 3205.5 9 3785.0 10 3487.5 11 3044.0 12 3121.0 13 3369.5 14 3604.5 15 3817.5 16 3938.0 17 4107.5 18 4351.0 19 4629.5 20 5037.5 21 5823.5 22 6835.5 23 8121.0 24 9738.5 25 11709.0 26 14486.0 27 17701.5 28 20154.5 29 22440.0 30 25346.5 31 29416.0 32 35840.0 33 38866.5 34 39975.0 35 43132.0 36 47651.0 37 52729.5 38 55794.0 39 58076.5 40 59972.0 41 63095.0 42 65284.0 43 67741.0 44 71506.0 45 74701.0 46 77007.0 47 78640.5 48 78078.5 49 77117.0 50 77126.5 51 74710.5 52 72892.5 53 73419.0 54 74946.0 55 71411.5 56 64856.5 57 59669.5 58 56141.5 59 50745.0 60 41002.0 61 33992.5 62 28865.0 63 21669.0 64 15886.0 65 12607.5 66 9300.5 67 6786.5 68 5181.0 69 3833.0 70 2789.5 71 1942.0 72 1420.0 73 1094.0 74 807.0 75 600.5 76 450.0 77 313.0 78 211.0 79 151.5 80 99.0 81 63.0 82 44.0 83 32.0 84 29.0 85 20.5 86 15.0 87 14.0 88 9.5 89 7.0 90 3.0 91 2.5 92 2.5 93 3.0 94 2.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3425086211758362 2 0.3024805052070939 3 0.30142592712315225 4 0.30394774428040405 5 0.31348479825692 6 0.2082103947469173 7 0.20568857758966552 8 0.20582613125278834 9 0.20408378485323253 10-11 0.21277259124049103 12-13 0.20302920676929084 14-15 0.2045193714531215 16-17 0.210823914346251 18-19 0.2033043140955365 20-21 0.19532620163441264 22-23 0.19489061503452368 24-25 0.19862748954936044 26-27 0.19323997107704977 28-29 0.20979226187282982 30-31 0.21527148278722236 32-33 0.21749526700770805 34-35 0.21508807790305862 36-37 0.18718760989964542 38-39 0.17939290232268532 40-41 0.18535356105800774 42-43 0.19656418460251807 44-45 0.20195170307482874 46-47 0.20603246174747256 48-49 0.2048174043898876 50-51 0.2098839643149117 52-53 0.23686740789750604 54-55 0.1952803504133717 56-57 0.17771933275469093 58-59 0.17659597783918785 60-61 0.16939733613575994 62-63 0.17732959737584292 64-65 0.17327176431371955 66-67 0.17329468992424005 68-69 0.18322147927960394 70-71 0.17751300226000669 72-73 0.17235473989290073 74-75 0.17911779499643965 76-77 0.1838175451531362 78-79 0.18670617207871554 80-81 0.17783396080729327 82-83 0.1733176155347605 84-85 0.16758621290464276 86-87 0.16380348716876506 88-89 0.1712543105879181 90-91 0.18042455479610647 92-93 0.16925978247263712 94-95 0.17540384609212337 96-97 0.17336346675580144 98-99 0.179232423049042 100-101 0.1533494087714303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180967.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.128341251835124 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95570110794091 36.824492628014774 2 13.321976308458058 13.089731964615806 3 4.700021306459306 6.927127519038863 4 2.1820845666193778 4.288087809169582 5 1.1962486609905691 2.9384856219597753 6 0.7803567442118502 2.3002579456686476 7 0.5139497151890431 1.7674647895863544 8 0.3732171781254392 1.466843271039481 9 0.25770702661770384 1.1394646872003282 >10 1.5164765980376926 13.911221113963798 >50 0.12298871593472824 4.138006708344076 >100 0.07123573861054833 6.351851583981016 >500 0.005606743817509564 1.9135054054606748 >1k 0.0021492517967119876 1.6868100360557876 >5k 1.868914605836511E-4 0.7166750530132787 >10k+ 9.344573029182554E-5 0.5399738628878473 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11762 0.539302061883559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9247 0.42398624096559 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6364 0.29179717070455446 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3035 0.13915845585925876 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2475 0.11348177207633127 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2454 0.11251889643447151 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2338 0.10720015479372225 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2235 0.10247747902650522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.585122104094193E-5 7 0.0 0.0 0.0 0.0 4.585122104094193E-5 8 0.0 0.0 0.0 0.0 4.585122104094193E-5 9 0.0 0.0 0.0 0.0 4.585122104094193E-5 10-11 0.0 0.0 0.0 0.0 4.585122104094193E-5 12-13 0.0 0.0 0.0 0.0 2.0633049468423868E-4 14-15 0.0 0.0 0.0 0.0 2.5218171572518065E-4 16-17 0.0 0.0 0.0 2.2925610520470965E-5 3.438841578070645E-4 18-19 0.0 0.0 0.0 4.585122104094193E-5 3.6680976832753544E-4 20-21 0.0 0.0 0.0 6.87768315614129E-5 4.1266098936847736E-4 22-23 0.0 0.0 0.0 9.170244208188386E-5 4.1266098936847736E-4 24-25 0.0 0.0 0.0 3.6680976832753544E-4 4.1266098936847736E-4 26-27 0.0 0.0 0.0 5.96065873532245E-4 4.1266098936847736E-4 28-29 0.0 0.0 0.0 0.0011004293049826064 4.1266098936847736E-4 30-31 0.0 4.585122104094193E-5 0.0 0.0027281476519360453 4.1266098936847736E-4 32-33 0.0 4.585122104094193E-5 0.0 0.004447568440971368 4.5851221040941933E-4 34-35 0.0 4.585122104094193E-5 0.0 0.0064420965562523415 5.502146524913032E-4 36-37 0.0 4.585122104094193E-5 0.0 0.008069814903205779 5.502146524913032E-4 38-39 0.0 4.585122104094193E-5 0.0 0.009720458860679689 5.502146524913032E-4 40-41 0.0 4.585122104094193E-5 0.0 0.011279400376071714 5.502146524913032E-4 42-43 0.0 4.585122104094193E-5 0.0 0.013136374828229862 5.502146524913032E-4 44-45 0.0 4.585122104094193E-5 0.0 0.016277183469534384 5.960658735322451E-4 46-47 0.0 4.585122104094193E-5 0.0 0.020610123857903395 6.648427050936581E-4 48-49 0.0 4.585122104094193E-5 0.0 0.025745460614488895 7.336195366550709E-4 50-51 0.0 4.585122104094193E-5 0.0 0.03211878033917982 7.794707576960128E-4 52-53 0.0 4.585122104094193E-5 0.0 0.04507175028324592 8.253219787369547E-4 54-55 0.0 4.585122104094193E-5 0.0 0.06414585823627776 9.170244208188387E-4 56-57 0.0 4.585122104094193E-5 0.0 0.08844700538797698 9.170244208188387E-4 58-59 0.0 4.585122104094193E-5 0.0 0.12068041377975916 9.170244208188387E-4 60-61 0.0 4.585122104094193E-5 0.0 0.1654312055157185 9.858012523802514E-4 62-63 0.0 4.585122104094193E-5 0.0 0.1975499858548983 0.0011004293049826064 64-65 0.0 4.585122104094193E-5 0.0 0.23345149192995585 0.0011004293049826064 66-67 0.0 4.585122104094193E-5 0.0 0.29314978172526224 0.0011004293049826064 68-69 0.0 4.585122104094193E-5 0.0 0.367956048853559 0.0011004293049826064 70-71 0.0 4.585122104094193E-5 0.0 0.4365036243097672 0.0011004293049826064 72-73 0.0 4.585122104094193E-5 0.0 0.5073437608180225 0.0011921317470644903 74-75 0.0 4.585122104094193E-5 0.0 0.6176847242530492 0.0013067597996668451 76-77 0.0 4.585122104094193E-5 0.0 0.7120465371553077 0.001352611020707787 78-79 0.0 4.585122104094193E-5 0.0 0.7951289496814945 0.001375536631228258 80-81 0.0 4.585122104094193E-5 0.0 0.8936861493089991 0.001375536631228258 82-83 0.0 4.585122104094193E-5 0.0 1.048227689827494 0.001375536631228258 84-85 0.0 4.585122104094193E-5 0.0 1.2127418709223936 0.001375536631228258 86-87 0.0 4.585122104094193E-5 0.0 1.3907133853928098 0.001398462241748729 88-89 0.0 4.585122104094193E-5 0.0 1.615521922156548 0.0014213878522692 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5490 0.0 57.17743 1 GTATCAA 9885 0.0 46.86356 1 TGGTATC 2030 0.0 35.364223 2 TATCAAC 13305 0.0 34.660915 2 ATCAACG 13355 0.0 34.565155 3 TCAACGC 13545 0.0 34.117752 4 CAACGCA 13750 0.0 33.47155 5 AACGCAG 14155 0.0 32.653275 6 GTGGTAT 2385 0.0 31.109228 1 CGCAGAG 16300 0.0 28.005903 8 TACATGG 11940 0.0 27.952198 2 ACGCAGA 16405 0.0 27.913517 7 GTACATG 12295 0.0 27.735989 1 ACATGGG 12155 0.0 26.048485 3 GCAGAGT 17690 0.0 25.21457 9 GGACCGT 260 2.3646862E-11 23.75165 6 ACCGTAT 120 1.7109615E-4 23.751104 8 GAGTACT 10580 0.0 23.70675 12-13 CATGGGA 6490 0.0 23.588718 4 GTACTTT 10950 0.0 22.73165 14-15 >>END_MODULE