FastQCFastQC Report
Thu 26 May 2016
SRR937903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180967
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138900.6368734602586834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115890.531369800643476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78090.3580521851087155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.1411759095850602No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25960.11902976982228525No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25890.11870881127499866No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23430.10742941089892694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.10344035466836499No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22540.10334865222628312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59900.060.225241
GTATCAA105400.045.0493431
CGTATAC459.610579E-442.236853
TGGTATC18850.038.3156552
ATCAACG145300.032.5060963
TATCAAC146800.032.3357932
GTGGTAT23800.032.1523361
TCAACGC147300.032.032484
CAACGCA150050.031.4454125
AACGCAG155850.030.2931466
ACGCAGA183300.025.731887
CGCAGAG183800.025.5585088
TAAGGTG10300.025.3728665
GTAAGGT10500.025.3421084
GTACATG120650.023.7549211
TACATGG119300.023.300192
GCAGAGT198650.023.2653169
ACATGGG118300.022.2118363
GAGTACT129600.021.86201312-13
AGTACTT131100.021.24956512-13
Produced by FastQC (version 0.11.5)