##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937903_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180967 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99392975684639 31.0 31.0 34.0 30.0 34.0 2 32.27155752471266 33.0 31.0 34.0 31.0 34.0 3 32.35361332839974 34.0 31.0 34.0 30.0 34.0 4 36.036168818693724 37.0 35.0 37.0 35.0 37.0 5 35.93254735170225 37.0 35.0 37.0 35.0 37.0 6 35.97113298825705 37.0 35.0 37.0 35.0 37.0 7 35.90951490783675 37.0 35.0 37.0 35.0 37.0 8 35.87602150789077 37.0 35.0 37.0 35.0 37.0 9 37.54379135493568 39.0 37.0 39.0 35.0 39.0 10-11 37.45175672075735 39.0 37.0 39.0 34.5 39.0 12-13 37.33355181440159 39.0 37.0 39.0 34.0 39.0 14-15 38.49090472253822 40.0 38.0 41.0 34.0 41.0 16-17 38.44698131608594 40.0 38.0 41.0 34.0 41.0 18-19 38.43953049266679 40.0 38.0 41.0 34.0 41.0 20-21 38.33793221080374 40.0 38.0 41.0 34.0 41.0 22-23 38.28264526698479 40.0 38.0 41.0 34.0 41.0 24-25 38.290199943419594 40.0 38.0 41.0 34.0 41.0 26-27 38.12847397507619 40.0 37.5 41.0 33.0 41.0 28-29 37.966510955920015 40.0 37.0 41.0 33.0 41.0 30-31 37.959594299225984 40.0 37.0 41.0 33.0 41.0 32-33 37.83911219197723 40.0 37.0 41.0 33.0 41.0 34-35 37.67031344353216 40.0 37.0 41.0 33.0 41.0 36-37 37.48853902878861 40.0 36.0 41.0 33.0 41.0 38-39 37.324033100913496 40.0 36.0 41.0 32.0 41.0 40-41 37.10507517995458 40.0 36.0 41.0 31.5 41.0 42-43 36.84384426724476 39.0 35.5 41.0 31.0 41.0 44-45 36.497895658210325 39.0 35.0 41.0 30.5 41.0 46-47 36.06559727863833 39.0 35.0 41.0 29.5 41.0 48-49 35.66425718500096 39.0 35.0 40.0 28.0 41.0 50-51 35.355705061103635 39.0 35.0 40.0 27.0 41.0 52-53 35.07397108713704 38.0 34.5 40.0 26.0 41.0 54-55 34.807744454638694 38.0 34.0 40.0 26.0 41.0 56-57 34.503517934934365 38.0 34.0 40.0 25.0 41.0 58-59 34.20505285040993 38.0 34.0 40.0 24.5 41.0 60-61 33.84554099167938 37.0 33.0 40.0 23.5 41.0 62-63 33.44298148481843 36.5 33.0 39.5 22.5 41.0 64-65 32.99975675927237 36.0 32.0 39.0 22.0 40.5 66-67 32.552825650273476 35.5 32.0 39.0 20.5 40.0 68-69 32.09053988437239 35.0 31.0 38.0 19.0 40.0 70-71 31.66758208629475 35.0 31.0 37.5 18.0 40.0 72-73 31.17320963590921 35.0 30.5 37.0 15.5 39.0 74-75 30.689954960345574 34.0 30.0 36.5 12.0 39.0 76-77 29.510100565483107 33.5 28.5 35.0 10.0 37.5 78-79 30.10145178721182 34.0 30.0 35.0 7.5 37.5 80-81 29.93084145702342 34.0 30.0 35.0 7.0 37.0 82-83 29.543319775127273 34.0 29.0 35.0 3.5 37.0 84-85 29.15528410104325 34.0 29.0 35.0 2.0 36.0 86-87 28.77111781150288 34.0 29.0 35.0 2.0 36.0 88-89 28.300697580476918 33.5 28.5 35.0 2.0 35.0 90-91 27.71165244591046 33.0 27.0 35.0 2.0 35.0 92-93 27.223197324856358 33.0 26.0 35.0 2.0 35.0 94-95 26.702071833273955 32.5 25.0 34.5 2.0 35.0 96-97 26.067072541675323 32.0 24.5 34.0 2.0 35.0 98-99 25.0957027318616 32.0 20.5 34.0 2.0 35.0 100-101 22.312729170134165 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3356468011244047 1101 2 0.26423603204044355 1101 3 0.20256470034686203 1101 4 0.09641286159485674 1101 5 -4.7023541822710513E-4 1101 6 0.11124617214063903 1101 7 0.1188545350797483 1101 8 0.09985590480710727 1101 9 0.10305622907075929 1101 10-11 0.18069494326123703 1101 12-13 0.1480419981162271 1101 14-15 0.1795353131577926 1101 16-17 0.3258052106076832 1101 18-19 0.3139285654468438 1101 20-21 0.23365181831353965 1101 22-23 0.2999894874233533 1101 24-25 0.2325680417584124 1101 26-27 0.058807910373566585 1101 28-29 0.031077325795145327 1101 30-31 0.09702718256810527 1101 32-33 0.2040471810030411 1101 34-35 0.14937541416300348 1101 36-37 0.19749283298946096 1101 38-39 0.14468428644412512 1101 40-41 0.21512311354823765 1101 42-43 0.08062884071507881 1101 44-45 0.04808716883862729 1101 46-47 -0.09486387947337249 1101 48-49 -0.03925914054742208 1101 50-51 0.07491648289787634 1101 52-53 -0.020990328490086085 1101 54-55 -0.3062978814484367 1101 56-57 -0.3359342714935707 1101 58-59 -0.29218925844126176 1101 60-61 -0.11305516629963819 1101 62-63 -0.3054102904394256 1101 64-65 -0.2779653314797699 1101 66-67 -0.2032296887286691 1101 68-69 -0.05355652741647532 1101 70-71 -0.12075930272390778 1101 72-73 -0.2369886865761579 1101 74-75 -0.2672828520589974 1101 76-77 -0.2639922107654016 1101 78-79 -0.3584602970870847 1101 80-81 -0.45819148011301536 1101 82-83 -0.3449059845391709 1101 84-85 -0.25743132821259707 1101 86-87 -0.37823532134854077 1101 88-89 -0.29698550375657007 1101 90-91 -0.3827462210360437 1101 92-93 -0.3100383773507325 1101 94-95 -0.7256816479974795 1101 96-97 -0.7946221176759707 1101 98-99 -0.8411516958231076 1101 100-101 -0.44118258141289246 1102 1 0.39225487892149147 1102 2 0.3093338810381425 1102 3 0.22613900355675298 1102 4 0.11035777688645965 1102 5 0.12716589624651675 1102 6 0.13146330078627244 1102 7 0.049577385914780336 1102 8 0.09462259293382402 1102 9 0.1648870357039769 1102 10-11 0.18587945557430174 1102 12-13 0.2204652490488499 1102 14-15 0.25086410166461093 1102 16-17 0.3024540330978098 1102 18-19 0.20978354256102705 1102 20-21 0.22605672531509668 1102 22-23 0.23854407972655167 1102 24-25 0.24993892135578477 1102 26-27 0.19860542159599248 1102 28-29 0.13026066809466386 1102 30-31 0.18862425106681968 1102 32-33 0.39220358653326315 1102 34-35 0.29958957367877304 1102 36-37 0.25115294699228485 1102 38-39 0.3148136640733483 1102 40-41 0.3735891023125788 1102 42-43 0.4576207299929891 1102 44-45 0.40869534070714764 1102 46-47 0.2710546252610655 1102 48-49 0.12079195200418269 1102 50-51 0.3052665756464421 1102 52-53 0.3341708129492673 1102 54-55 0.2787400558000357 1102 56-57 0.3201240763179456 1102 58-59 0.17897255325711114 1102 60-61 0.20221115877010476 1102 62-63 0.07265518916323543 1102 64-65 0.04245993022991712 1102 66-67 0.0037168992707563575 1102 68-69 0.10776805727280703 1102 70-71 -0.027181442437640868 1102 72-73 -0.14188334126047408 1102 74-75 -0.18785686423536774 1102 76-77 -0.10312534243308491 1102 78-79 -0.12359830283131856 1102 80-81 0.0821207782777158 1102 82-83 0.04459476068302948 1102 84-85 0.10813940616198892 1102 86-87 -0.21601000260803715 1102 88-89 -0.10048760713520721 1102 90-91 0.06565294760759599 1102 92-93 -0.08629680335913648 1102 94-95 -0.19361445747047412 1102 96-97 -0.17025349873292228 1102 98-99 -0.07957987962694801 1102 100-101 0.04253953154927004 1103 1 0.3036712267149966 1103 2 0.20962176220520945 1103 3 0.21941498104417434 1103 4 0.12020248634686936 1103 5 0.10544340267218644 1103 6 0.11920700114742999 1103 7 0.15549010695654886 1103 8 0.16370856334128803 1103 9 0.09660933643924352 1103 10-11 0.1539604589985757 1103 12-13 0.1590250718573074 1103 14-15 0.19843419177555432 1103 16-17 0.1343865284076884 1103 18-19 0.12490460416758964 1103 20-21 0.06826974635934846 1103 22-23 0.11222760879152816 1103 24-25 0.13479251518714364 1103 26-27 0.0660638303431682 1103 28-29 0.08025915293062269 1103 30-31 0.11444870691631337 1103 32-33 0.18937353559619652 1103 34-35 0.1378436088360857 1103 36-37 0.08363806394917361 1103 38-39 0.05726993781664902 1103 40-41 0.019942102475120294 1103 42-43 0.07160431663420042 1103 44-45 0.12684562845057457 1103 46-47 0.09118426820577241 1103 48-49 0.18602472252294433 1103 50-51 0.2103321822492461 1103 52-53 0.14233892453145813 1103 54-55 0.021901294047182773 1103 56-57 0.03815012061032519 1103 58-59 0.024323341280151567 1103 60-61 0.18138013261837926 1103 62-63 -0.0016845436720274165 1103 64-65 0.08732669221417666 1103 66-67 -0.07212504983626644 1103 68-69 0.03937327715065919 1103 70-71 -0.06035802516421995 1103 72-73 -0.12746889706396658 1103 74-75 -0.014309148964994023 1103 76-77 0.005057920789475645 1103 78-79 -0.006717722551041305 1103 80-81 0.13784217542425026 1103 82-83 0.0983987866116145 1103 84-85 0.09400733344513412 1103 86-87 -0.09812473082893547 1103 88-89 -0.11707892882656878 1103 90-91 -0.15929849827212195 1103 92-93 -0.08558605368351735 1103 94-95 -0.25790204111441284 1103 96-97 -0.2621358007905137 1103 98-99 -0.11752950455497313 1103 100-101 0.042724106077436375 1104 1 0.2619481969213666 1104 2 0.20168955780312814 1104 3 0.12426436173043953 1104 4 0.11588560217606414 1104 5 0.06687178966357266 1104 6 0.07430708467113334 1104 7 0.11773493590862216 1104 8 0.08239986730343674 1104 9 0.1375510551655026 1104 10-11 0.11030593269036615 1104 12-13 0.1550594087924324 1104 14-15 0.08580041992084375 1104 16-17 0.08069949724044534 1104 18-19 0.08882043406029538 1104 20-21 -0.006639506151849162 1104 22-23 0.07210110244469092 1104 24-25 -0.009681878343855033 1104 26-27 0.036988097798179354 1104 28-29 -0.002523020600179393 1104 30-31 0.07647879828298443 1104 32-33 0.038334319858265076 1104 34-35 -0.009237936115205514 1104 36-37 0.009643005109317926 1104 38-39 -0.004826814420084702 1104 40-41 -0.14165113623315762 1104 42-43 0.1364015806402321 1104 44-45 -0.12122572941993326 1104 46-47 -0.22540102652924787 1104 48-49 -0.2578713286768206 1104 50-51 -0.00856375916646357 1104 52-53 0.03496029518083077 1104 54-55 -0.08662251459835346 1104 56-57 0.01575145347641893 1104 58-59 -0.06295549876801232 1104 60-61 -0.1971001751985071 1104 62-63 -0.2380770751954131 1104 64-65 -0.13723173441363912 1104 66-67 -0.26518178008106474 1104 68-69 -0.09221896910987937 1104 70-71 -0.2059689909212601 1104 72-73 -0.3809012292546683 1104 74-75 -0.40338388027953087 1104 76-77 -0.3463883741747722 1104 78-79 -0.3385532502477986 1104 80-81 -0.3131611189752732 1104 82-83 -0.3765444550388679 1104 84-85 -0.32656540550861735 1104 86-87 -0.2322817079560906 1104 88-89 -0.16160013427291275 1104 90-91 -0.23261280822672603 1104 92-93 -0.18765754680796576 1104 94-95 -0.08938933383116421 1104 96-97 0.039705321501003255 1104 98-99 0.17893632757731837 1104 100-101 -0.021624272939231304 1105 1 0.24924991002952623 1105 2 0.16227372806471863 1105 3 0.13462830757825373 1105 4 0.08020434068834703 1105 5 0.05425276091551012 1105 6 0.09012474565007977 1105 7 0.07380894036972308 1105 8 0.02800424839168869 1105 9 0.21444089011985312 1105 10-11 0.11759862546693256 1105 12-13 0.1620831900097599 1105 14-15 0.24300045615313337 1105 16-17 0.14961917099881816 1105 18-19 0.17065069330991633 1105 20-21 0.048802508967590086 1105 22-23 0.05703261963427053 1105 24-25 0.14973078106880422 1105 26-27 0.1192957930091012 1105 28-29 0.09034110452201105 1105 30-31 0.00436521004036905 1105 32-33 0.0100646383627776 1105 34-35 0.01289112633643441 1105 36-37 0.12168193972890151 1105 38-39 0.21793291640415902 1105 40-41 0.31955011529067434 1105 42-43 0.30783505852532045 1105 44-45 0.315146021467946 1105 46-47 0.15674966207305374 1105 48-49 0.13032184983894268 1105 50-51 0.36752651208509235 1105 52-53 0.3606376692452997 1105 54-55 0.15311732998019778 1105 56-57 0.1179944589452333 1105 58-59 0.25772389449202393 1105 60-61 0.13036616839686843 1105 62-63 -0.0022642829310086654 1105 64-65 0.12188955696571924 1105 66-67 0.1930048313313506 1105 68-69 0.16973580090940743 1105 70-71 0.16749160834579513 1105 72-73 0.2820827643744863 1105 74-75 0.18806209019869513 1105 76-77 0.24350357055588034 1105 78-79 0.16645794024726257 1105 80-81 0.05459949878534687 1105 82-83 -0.00925938614089361 1105 84-85 0.057519559221510974 1105 86-87 0.001739284129421037 1105 88-89 0.06094918505059965 1105 90-91 0.30260328344097687 1105 92-93 0.4188682290726504 1105 94-95 0.134127123556258 1105 96-97 -0.15521498632253028 1105 98-99 -0.12626319536413888 1105 100-101 -0.14732014057058151 1106 1 0.10263550873028748 1106 2 0.1245630655670027 1106 3 0.079212158891238 1106 4 0.03444075148352965 1106 5 -0.025602958207670667 1106 6 -0.031023892534328468 1106 7 -0.006669400755974664 1106 8 -0.1312014184800745 1106 9 -0.049670828847062864 1106 10-11 -0.09039130654344518 1106 12-13 -0.18579107034319975 1106 14-15 0.05324724467931219 1106 16-17 0.021436125013394758 1106 18-19 0.052698007001175995 1106 20-21 0.17238181461673463 1106 22-23 0.20292882558157999 1106 24-25 0.10664812895719677 1106 26-27 0.17870003618676833 1106 28-29 0.11171971171833661 1106 30-31 0.17236275303790904 1106 32-33 0.15064536156700825 1106 34-35 -0.2001898305690517 1106 36-37 0.03714521933186887 1106 38-39 0.08640602401695929 1106 40-41 0.01254287962074585 1106 42-43 0.047045211065970705 1106 44-45 0.12863742056411098 1106 46-47 0.16763159480336753 1106 48-49 -0.006169624599721146 1106 50-51 0.04980634108552806 1106 52-53 0.12968414719296817 1106 54-55 0.04537111001081229 1106 56-57 -0.02877324340168741 1106 58-59 0.11916573357441251 1106 60-61 0.1284632472009335 1106 62-63 0.04760250735316163 1106 64-65 -0.1865711609924432 1106 66-67 -0.21574145560914815 1106 68-69 0.04578205003913638 1106 70-71 0.08949608145759314 1106 72-73 0.18703070829176482 1106 74-75 0.055791190679073566 1106 76-77 -0.06385491461350767 1106 78-79 -0.07541832505319235 1106 80-81 -0.15545851493536134 1106 82-83 -0.209307312338737 1106 84-85 -0.37637943579332855 1106 86-87 -0.11439175321115869 1106 88-89 -0.008248361574541008 1106 90-91 -0.2423379912703112 1106 92-93 -0.161959094301519 1106 94-95 -0.1503368355243957 1106 96-97 -0.27157065591499574 1106 98-99 -0.40204096709591397 1106 100-101 -0.9465892486131189 1107 1 0.09159225237187485 1107 2 0.057441623232612926 1107 3 0.02733920028914838 1107 4 -0.025856265372262044 1107 5 -0.07473986807711697 1107 6 -0.057295401770062426 1107 7 0.04596291252369866 1107 8 0.01530973569717986 1107 9 0.033441709120673124 1107 10-11 0.04736930523761629 1107 12-13 0.07997835790492758 1107 14-15 0.041116754269438616 1107 16-17 0.047244068623719215 1107 18-19 0.08547460813382202 1107 20-21 0.03242249258756402 1107 22-23 0.11047242307407146 1107 24-25 0.01889540023342562 1107 26-27 0.08899276270607004 1107 28-29 0.05553356971446988 1107 30-31 0.033849239524393226 1107 32-33 0.012726391024528994 1107 34-35 -0.11180174835643442 1107 36-37 -0.07610675805980094 1107 38-39 -0.030224331753082367 1107 40-41 -0.0999830542424931 1107 42-43 0.026713027403296508 1107 44-45 -0.026377320055395614 1107 46-47 0.06645482177821549 1107 48-49 0.16471988423697326 1107 50-51 0.15438154894012257 1107 52-53 0.1092044178120517 1107 54-55 0.13980856667144081 1107 56-57 0.038960879911655866 1107 58-59 -0.0021041153359888654 1107 60-61 -0.047561759531063785 1107 62-63 0.04216651149978645 1107 64-65 0.08012051519667551 1107 66-67 -0.10531621698969929 1107 68-69 0.03869566986927708 1107 70-71 -0.0407393498851647 1107 72-73 -0.08646760417587984 1107 74-75 -0.12172496958836376 1107 76-77 -0.10457177751946745 1107 78-79 -0.15991388612456348 1107 80-81 -0.16775922357337691 1107 82-83 -0.23714636402787548 1107 84-85 -0.07474907304712275 1107 86-87 -0.3295465471564114 1107 88-89 -0.3446609705595556 1107 90-91 -0.20968172884403202 1107 92-93 -0.24206543186101115 1107 94-95 -0.279058789629655 1107 96-97 -0.257794838902381 1107 98-99 -0.26160308070402394 1107 100-101 -0.09265183590227721 1108 1 0.1490965866289713 1108 2 0.03158869885570681 1108 3 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0.2265083382646793 2316 78-79 0.07801475397798896 2316 80-81 -0.08102761601934816 2316 82-83 -0.08724540349307475 2316 84-85 -0.14020092425067432 2316 86-87 -0.1586885612119957 2316 88-89 -0.2588998081876035 2316 90-91 -0.01953979158318475 2316 92-93 0.03983441407759969 2316 94-95 0.26196171799640666 2316 96-97 0.21808807529902197 2316 98-99 0.34055429358535605 2316 100-101 0.6502150487927878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 25.0 5 63.0 6 136.0 7 314.0 8 705.0 9 1454.0 10 2429.0 11 3977.0 12 6076.0 13 8393.0 14 10703.0 15 14917.0 16 20351.0 17 27259.0 18 24344.0 19 16068.0 20 13509.0 21 13663.0 22 14005.0 23 15406.0 24 17837.0 25 21050.0 26 25623.0 27 31360.0 28 38483.0 29 47767.0 30 58825.0 31 72788.0 32 90884.0 33 117966.0 34 160633.0 35 225540.0 36 329854.0 37 444415.0 38 284656.0 39 19478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25558806979601 19.34207503222762 15.278024271490697 22.124312626485672 2 16.081857267899974 20.505170412940682 39.58459710761327 23.82837521154607 3 19.62927453739557 24.563186879948205 29.70274194886947 26.10479663378676 4 13.208177840379978 16.67361312665437 35.81869876985759 34.29951026310806 5 13.342137420827433 37.96209991706604 33.576691889352894 15.119070772753625 6 32.555054707384386 36.10980817224653 16.44784171424877 14.887295406120312 7 28.91809000319583 33.44236753696869 19.578563086924287 18.06097937291119 8 27.28055949493963 34.68374349543116 18.746500978694314 19.289196030934903 9 26.977574626301088 16.50382605513976 19.85133200089685 36.66726731766231 10-11 25.397747879724914 27.11347764546644 25.806832473852193 21.681942000956454 12-13 26.443230260687272 24.22771172673162 27.22240792630895 22.106650086272158 14-15 23.139254284911235 25.251161526057018 26.135952538484077 25.473631650547667 16-17 21.92364671267378 28.724483222350454 26.95898195616898 22.392888108806783 18-19 21.886736479735823 26.47089570818816 30.20614250467797 21.43622530739805 20-21 22.508479037050996 25.267415783916036 31.0060170557372 21.218088123295768 22-23 22.415257588731503 25.201752907853127 30.849504630441587 21.533484872973787 24-25 22.082508104796897 25.52844238653456 30.83488635631578 21.55416315235276 26-27 21.343219902807895 26.040234513719668 30.761367182064053 21.855178401408384 28-29 21.648350061177624 26.286455249090473 30.598348559534642 21.46684613019726 30-31 21.62332121485561 25.83083558806713 30.70720464821338 21.838638548863877 32-33 21.438838826997383 25.75492889163385 30.609449844954096 22.196782436414672 34-35 22.185159151880793 25.948948333468596 30.45238648727835 21.41350602737226 36-37 22.326380912686894 25.57150108185956 30.244038538868313 21.858079466585234 38-39 21.852416840786677 25.978659924703123 30.254355063602524 21.914568170907675 40-41 22.208606903799406 25.849918739856808 30.137827015048657 21.803647341295125 42-43 21.825701804988597 25.930558243233698 29.999623979815972 22.244115971961733 44-45 21.989489066088574 26.18226227173543 30.10820429653452 21.72004436564148 46-47 22.470284969923892 26.58387311683304 29.05750064077081 21.888341272472257 48-49 22.303982591208396 26.721449705566386 28.809743567876083 22.164824135349136 50-51 22.694382812761496 27.080602319980084 28.501325329544187 21.723689537714233 52-53 22.783999024286018 26.82239116868802 28.460907478196596 21.932702328829368 54-55 22.458947090417734 27.098680126145936 28.475363669126434 21.9670091143099 56-57 22.541079255211105 27.34195427991345 28.227639391150806 21.889327073724637 58-59 22.525008402236253 26.758612120220064 28.42209441958544 22.294285057958234 60-61 22.261684839798125 27.058364477775225 28.727440626107594 21.952510056319056 62-63 22.181766161523765 27.110038803888365 28.97904920156976 21.72914583301811 64-65 22.083002631401573 26.94857372899269 28.82583734646145 22.14258629314428 66-67 22.087794084000354 27.114463446718815 28.524755303496107 22.27298716578472 68-69 22.436377991964115 26.867760951908032 28.740141414335934 21.95571964179192 70-71 22.40437383967754 26.981357352036962 28.343207393784496 22.271061414501 72-73 22.28227203804551 27.21682629769272 28.12270887179861 22.37819279246316 74-75 22.232408835163483 27.656998019685762 27.84136577949139 22.26922736565936 76-77 22.40684980561375 27.194221645719534 28.17087557950212 22.228052969164597 78-79 22.423929385451498 27.290463358684473 28.167780622081857 22.11782663378217 80-81 22.524985476625737 27.597414357943062 27.725385115868328 22.152215049562876 82-83 22.544906912381542 27.536318935365962 27.739028205979395 22.1797459462731 84-85 22.51025463028768 27.61750985572448 27.734292969262242 22.137942544725593 86-87 22.542959155273785 27.686388652373005 27.508669319618317 22.261982872734894 88-89 22.43715746272181 27.694481392886733 27.47324925136419 22.395111893027266 90-91 22.482578323313945 27.652573946255966 27.48381990366804 22.381027826762054 92-93 22.362080815871003 27.30150353082832 27.770454204691298 22.56596144860938 94-95 22.245980796591603 27.28149944497097 27.76000278775424 22.71251697068319 96-97 22.483169163036397 27.16263015442233 27.59149955042878 22.762701132112497 98-99 22.146312073549904 27.33946035220577 27.495906583442654 23.018320990801676 100-101 22.148026195105842 27.145389257805334 27.95786403492681 22.748720512162013 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1088.0 1 747.5 2 558.0 3 712.5 4 586.0 5 558.5 6 1086.0 7 2331.5 8 3712.0 9 4380.0 10 3922.5 11 3296.0 12 3388.0 13 3681.5 14 3975.5 15 4248.5 16 4432.0 17 4545.5 18 4676.5 19 4997.5 20 5476.5 21 6228.5 22 7056.5 23 8325.5 24 10019.0 25 11784.0 26 14508.5 27 17724.0 28 20110.5 29 22484.5 30 25612.5 31 29904.0 32 36199.5 33 39147.5 34 40158.5 35 43231.0 36 47896.0 37 53102.0 38 56332.0 39 58673.5 40 60301.5 41 63068.5 42 65141.5 43 68080.0 44 72551.5 45 75353.0 46 77177.5 47 79481.5 48 78647.5 49 76678.0 50 77493.0 51 75537.5 52 73212.5 53 74109.0 54 76086.5 55 71659.5 56 64542.5 57 59545.0 58 55363.0 59 49521.5 60 39529.5 61 32453.5 62 27700.0 63 20358.5 64 14361.5 65 11218.5 66 8180.5 67 6152.5 68 4816.0 69 3525.0 70 2500.5 71 1647.0 72 1138.0 73 939.0 74 740.5 75 558.0 76 430.0 77 268.0 78 176.5 79 135.5 80 85.0 81 51.5 82 35.0 83 24.0 84 16.0 85 9.5 86 5.5 87 4.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017606868879721703 2 0.0 3 0.0 4 0.0 5 0.04213727213662563 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003576395241193471 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.940988102983678E-4 24-25 2.5218171572518065E-4 26-27 0.001306759799666845 28-29 0.0010316524734211934 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.897353788480064E-4 42-43 0.01068333450253947 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.585122104094193E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.043634314503613E-4 84-85 4.585122104094193E-5 86-87 0.0 88-89 0.0 90-91 0.012769565059902326 92-93 0.0015589415153920256 94-95 0.0 96-97 0.0 98-99 0.0011921317470644903 100-101 0.018661446963663366 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180967.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.76914430061202 #Duplication Level Percentage of deduplicated Percentage of total 1 72.83658987895181 34.06504982413195 2 14.376528188211346 13.447558427525456 3 5.141597186683119 7.214043022788108 4 2.36418288162315 4.422832413746794 5 1.3470774055224 3.1500828781485595 6 0.8269473629820742 2.320537232899153 7 0.5431194665244969 1.7780862891648936 8 0.3918842533581683 1.4662472955556618 9 0.2835250439919537 1.1934201325767348 >10 1.667354078084141 14.4890038268078 >50 0.13701430839930379 4.430739540548767 >100 0.07586254248940909 6.706675129716684 >500 0.005381849115621916 1.77282328447367 >1k 0.002641998656759846 2.0194936728084576 >5k 9.785180210221652E-5 0.35737459415702605 >10k+ 1.9570360420443305E-4 1.1660324349502966 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13890 0.6368734602586834 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11589 0.531369800643476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7809 0.3580521851087155 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3079 0.1411759095850602 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2596 0.11902976982228525 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2589 0.11870881127499866 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2343 0.10742941089892694 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 0.10344035466836499 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2254 0.10334865222628312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.375536631228258E-4 14-15 0.0 0.0 0.0 0.0 1.8340488416376772E-4 16-17 0.0 0.0 0.0 2.2925610520470965E-5 2.751073262456516E-4 18-19 0.0 0.0 0.0 4.585122104094193E-5 2.751073262456516E-4 20-21 0.0 0.0 0.0 6.87768315614129E-5 3.438841578070645E-4 22-23 0.0 0.0 0.0 9.170244208188386E-5 4.5851221040941933E-4 24-25 0.0 0.0 0.0 3.6680976832753544E-4 4.5851221040941933E-4 26-27 0.0 0.0 0.0 5.96065873532245E-4 5.272890419708322E-4 28-29 0.0 0.0 0.0 0.0011004293049826064 5.502146524913032E-4 30-31 0.0 0.0 0.0 0.0027739988729769864 5.502146524913032E-4 32-33 0.0 0.0 0.0 0.004401717219930426 5.960658735322451E-4 34-35 0.0 0.0 0.0 0.0064420965562523415 6.41917094573187E-4 36-37 0.0 0.0 0.0 0.008069814903205779 6.41917094573187E-4 38-39 0.0 0.0 0.0 0.00976631008172063 7.336195366550709E-4 40-41 0.0 0.0 0.0 0.011302325986592185 7.336195366550709E-4 42-43 0.0 0.0 0.0 0.013205151659791275 7.336195366550709E-4 44-45 0.0 0.0 0.0 0.01630010908005486 7.565451471755419E-4 46-47 0.0 0.0 0.0 0.020724751910505754 8.253219787369547E-4 48-49 0.0 0.0 0.0 0.02611227038281643 9.399500313393096E-4 50-51 0.0 0.0 0.0 0.03241681327594595 0.0010087268629007224 52-53 0.0 4.585122104094193E-5 0.0 0.045392708830532515 0.0011462805260235485 54-55 0.0 4.585122104094193E-5 0.0 0.0646731472782486 0.001237982968105432 56-57 0.0 4.585122104094193E-5 0.0 0.08934110419827535 0.001260908578625903 58-59 0.0 4.585122104094193E-5 0.0 0.12203302480046696 0.001283834189146374 60-61 0.0 4.585122104094193E-5 0.0 0.167402808020479 0.001329685410187316 62-63 0.0 4.585122104094193E-5 0.0 0.1994528115280974 0.001329685410187316 64-65 0.0 4.585122104094193E-5 0.0 0.23551479687679822 0.001375536631228258 66-67 0.0 4.585122104094193E-5 0.0 0.2957862269351164 0.0014213878522692 68-69 0.0 4.585122104094193E-5 0.0 0.37148659287371155 0.0014443134627896708 70-71 0.0 4.585122104094193E-5 0.0 0.4414555561821889 0.0014672390733101418 72-73 0.0 4.585122104094193E-5 0.0 0.512822981732415 0.0015130902943510838 74-75 0.0 4.585122104094193E-5 0.0 0.6234390524936875 0.0015589415153920256 76-77 0.0 4.585122104094193E-5 0.0 0.718534484932601 0.0016047927364329676 78-79 0.0 4.585122104094193E-5 0.0 0.8025797731006475 0.0016047927364329676 80-81 0.0 4.585122104094193E-5 0.0 0.9026271374119829 0.0016506439574739094 82-83 0.0 4.585122104094193E-5 0.0 1.0595987926456476 0.0018340488416376773 84-85 0.0 4.585122104094193E-5 0.0 1.2262221299084306 0.0018340488416376773 86-87 0.0 4.585122104094193E-5 0.0 1.4086182872092974 0.0018569744521581481 88-89 0.0 4.585122104094193E-5 0.0 1.6398918461398087 0.0018799000626786191 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5990 0.0 60.22524 1 GTATCAA 10540 0.0 45.049343 1 CGTATAC 45 9.610579E-4 42.23685 3 TGGTATC 1885 0.0 38.315655 2 ATCAACG 14530 0.0 32.506096 3 TATCAAC 14680 0.0 32.335793 2 GTGGTAT 2380 0.0 32.152336 1 TCAACGC 14730 0.0 32.03248 4 CAACGCA 15005 0.0 31.445412 5 AACGCAG 15585 0.0 30.293146 6 ACGCAGA 18330 0.0 25.73188 7 CGCAGAG 18380 0.0 25.558508 8 TAAGGTG 1030 0.0 25.372866 5 GTAAGGT 1050 0.0 25.342108 4 GTACATG 12065 0.0 23.754921 1 TACATGG 11930 0.0 23.30019 2 GCAGAGT 19865 0.0 23.265316 9 ACATGGG 11830 0.0 22.211836 3 GAGTACT 12960 0.0 21.862013 12-13 AGTACTT 13110 0.0 21.249565 12-13 >>END_MODULE