FastQCFastQC Report
Thu 26 May 2016
SRR937902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101489
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113880.541901480331327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89450.4256505744260379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58800.2798016073365123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29300.13942495059455462No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.11077859555772122No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23240.1105882543282406No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22050.10492560275119213No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21310.10140429000580066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49300.061.345131
GTATCAA94900.047.7525521
ATCAACG125050.035.879873
TATCAAC126700.035.4134562
TCAACGC127400.035.223084
CAACGCA129450.034.6269045
AACGCAG133050.033.742926
TGGTATC16750.030.9303342
CGCAGAG153350.028.9973138
ACGCAGA155050.028.7713017
GTACATG112550.028.645351
TACATGG110350.028.55712
GGACCGA7350.028.4400966
ACATGGG112500.026.574233
GCAGAGT163950.026.2532149
GTGGTAT20850.025.9997231
ACCGAGT8750.025.518528
TAGCGCG1501.0537824E-625.3526574
GAGTACT101150.023.45815512-13
CATGGGA59450.022.9485264
Produced by FastQC (version 0.11.5)