##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937902_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.386317511060014 31.0 31.0 34.0 30.0 34.0 2 31.490865286470687 33.0 31.0 34.0 28.0 34.0 3 31.52710482900458 33.0 31.0 34.0 28.0 34.0 4 35.150253463139705 37.0 35.0 37.0 33.0 37.0 5 35.125730850839574 37.0 35.0 37.0 32.0 37.0 6 35.20929683667152 37.0 35.0 37.0 32.0 37.0 7 35.12944345652059 37.0 35.0 37.0 32.0 37.0 8 35.11045406376146 37.0 35.0 37.0 32.0 37.0 9 36.666582599290315 39.0 37.0 39.0 32.0 39.0 10-11 36.538777980755555 39.0 37.0 39.0 32.0 39.0 12-13 36.38401176499139 39.0 37.0 39.0 32.0 39.0 14-15 37.428710547616475 40.0 37.0 41.0 32.0 41.0 16-17 37.358333781428314 40.0 37.0 41.0 32.0 41.0 18-19 37.32483705601123 40.0 37.0 41.0 32.0 41.0 20-21 37.156327965552045 40.0 37.0 41.0 31.0 41.0 22-23 36.99339611104317 39.0 36.0 41.0 31.0 41.0 24-25 36.82437286133784 39.0 36.0 41.0 30.5 41.0 26-27 36.89793831897288 39.0 36.0 41.0 31.0 41.0 28-29 36.82312374701938 39.0 36.0 41.0 30.0 41.0 30-31 36.56976101231079 39.0 36.0 41.0 30.0 41.0 32-33 36.28095935786483 39.0 35.0 40.0 30.0 41.0 34-35 35.973714352061805 38.5 35.0 40.0 29.0 41.0 36-37 35.94889671085597 39.0 35.0 40.0 29.0 41.0 38-39 35.863319769934556 39.0 35.0 40.0 28.5 41.0 40-41 35.63025978246853 39.0 35.0 40.0 27.0 41.0 42-43 35.32906310715878 38.0 35.0 40.0 26.0 41.0 44-45 35.04855509593436 38.0 34.5 40.0 25.5 41.0 46-47 34.62122047748049 38.0 34.0 40.0 23.5 41.0 48-49 34.21141771382101 38.0 33.5 40.0 22.5 41.0 50-51 33.26235040963812 37.5 32.0 39.5 19.5 40.5 52-53 33.33078855040402 37.5 33.0 39.5 20.0 40.5 54-55 33.59297550451132 38.0 33.0 40.0 18.5 41.0 56-57 33.27730789930378 38.0 33.0 40.0 16.5 41.0 58-59 33.00763054196334 37.0 32.5 40.0 13.5 41.0 60-61 32.65878193985312 37.0 32.0 40.0 11.5 41.0 62-63 32.26027592816332 36.0 31.0 39.0 8.0 41.0 64-65 31.855648542533416 36.0 31.0 39.0 7.0 41.0 66-67 31.421381934428396 35.0 31.0 39.0 4.0 40.0 68-69 30.90816344982058 35.0 30.0 38.0 2.0 40.0 70-71 30.43839582315206 35.0 29.5 37.5 2.0 40.0 72-73 29.965381213035137 34.5 29.0 37.0 2.0 39.0 74-75 29.464617230925313 34.0 29.0 36.5 2.0 39.0 76-77 28.99611109075517 34.0 28.0 36.0 2.0 38.5 78-79 28.451017112152382 34.0 27.0 35.5 2.0 37.5 80-81 27.925383382925155 33.5 26.0 35.0 2.0 37.0 82-83 27.393699181865813 33.0 26.0 35.0 2.0 36.5 84-85 26.91773333098579 33.0 25.0 35.0 2.0 36.0 86-87 26.36631502710697 32.5 24.5 35.0 2.0 35.5 88-89 25.838651784520405 32.0 22.5 35.0 2.0 35.0 90-91 25.293114786706 32.0 19.5 34.5 2.0 35.0 92-93 24.69373953420646 31.0 17.5 34.0 2.0 35.0 94-95 24.007029063678182 31.0 7.5 34.0 2.0 35.0 96-97 23.174480094828 31.0 2.0 34.0 2.0 35.0 98-99 22.180876749771237 30.5 2.0 34.0 2.0 35.0 100-101 19.716227636689986 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2698466921938305 1101 2 0.39724310500441007 1101 3 0.34309567402576135 1101 4 0.3415238354532235 1101 5 0.3440090605065862 1101 6 0.3024508492818896 1101 7 0.19150539862223326 1101 8 0.36724202677042683 1101 9 0.4785282607887922 1101 10-11 0.38517909582848375 1101 12-13 0.41977367881197125 1101 14-15 0.6245668113889522 1101 16-17 0.7126105453867879 1101 18-19 0.6814685159152916 1101 20-21 0.7795354923916307 1101 22-23 0.794877027988683 1101 24-25 0.8107317553829319 1101 26-27 0.7456640705992825 1101 28-29 0.6763645085747498 1101 30-31 0.6915579503988383 1101 32-33 0.6885473903572006 1101 34-35 0.6387544452522249 1101 36-37 0.6590736323296298 1101 38-39 0.6699303469560789 1101 40-41 0.847457719659495 1101 42-43 0.9709028120242422 1101 44-45 0.8361986407312187 1101 46-47 0.9087711413213242 1101 48-49 1.0271346694189916 1101 50-51 0.8006734787089229 1101 52-53 0.7996901532731826 1101 54-55 1.0260165476205643 1101 56-57 0.9628450666891908 1101 58-59 0.9974009271228326 1101 60-61 1.0517674886724322 1101 62-63 0.9274825201079295 1101 64-65 0.8907295809050559 1101 66-67 0.9515917561528049 1101 68-69 1.2083812328637613 1101 70-71 1.1637661600818028 1101 72-73 1.1914417569510363 1101 74-75 1.0260394702796418 1101 76-77 1.071491666667061 1101 78-79 1.1478603109301027 1101 80-81 1.1651372940531104 1101 82-83 1.3054301317899082 1101 84-85 1.2978591404412292 1101 86-87 1.3998705701457794 1101 88-89 1.4860449217538445 1101 90-91 1.5697453118851108 1101 92-93 1.823797558540143 1101 94-95 1.878631323705342 1101 96-97 2.090240904987489 1101 98-99 2.536437981513295 1101 100-101 2.509840305584209 1102 1 0.23724138905970094 1102 2 0.39819786724097384 1102 3 0.3372214101082882 1102 4 0.1654128158385646 1102 5 0.10849048487210666 1102 6 0.2804479370075299 1102 7 0.28302283330241096 1102 8 0.22872165102330655 1102 9 0.22349406863012433 1102 10-11 0.3125676425974362 1102 12-13 0.3457188531165514 1102 14-15 0.5423114725712068 1102 16-17 0.5597908104091331 1102 18-19 0.46282220522483186 1102 20-21 0.3716178376593007 1102 22-23 0.5061609778097278 1102 24-25 0.5499208102067072 1102 26-27 0.6085439744089953 1102 28-29 0.6352583856196432 1102 30-31 0.5743857434622868 1102 32-33 0.43352442730807184 1102 34-35 0.5972773777812037 1102 36-37 0.5605137224389054 1102 38-39 0.7759421626666168 1102 40-41 0.8108592683936351 1102 42-43 0.7247921148865686 1102 44-45 0.6360338127592584 1102 46-47 0.5789514635449393 1102 48-49 0.5811962095460714 1102 50-51 0.5173840636706686 1102 52-53 0.5596957697474565 1102 54-55 0.5233253043681074 1102 56-57 0.6501854837671104 1102 58-59 0.6739572721071809 1102 60-61 0.7237961031308444 1102 62-63 0.603417329080628 1102 64-65 0.6660948916711398 1102 66-67 0.6674549752333085 1102 68-69 0.6928794951350632 1102 70-71 0.814614178595054 1102 72-73 0.6900687343780305 1102 74-75 0.6125640781533761 1102 76-77 0.8539952359882896 1102 78-79 0.782427560243594 1102 80-81 0.8476402683275097 1102 82-83 0.8989959671953471 1102 84-85 1.0775781785230727 1102 86-87 1.076290918136845 1102 88-89 0.9648953258290334 1102 90-91 1.292069011986115 1102 92-93 1.5726161986316356 1102 94-95 1.6409167373415876 1102 96-97 1.6361353313949785 1102 98-99 1.8410055993719183 1102 100-101 1.8669158154913497 1103 1 0.03583766145137446 1103 2 0.11613323984441948 1103 3 -0.022979369203564914 1103 4 0.11940772242142828 1103 5 0.034348983331561556 1103 6 -0.015088112570936119 1103 7 -0.016751264350943984 1103 8 -0.023587699035289234 1103 9 -0.06795033750510271 1103 10-11 0.01461280549820998 1103 12-13 0.016661999073917855 1103 14-15 0.20082706137036865 1103 16-17 0.06539957184791234 1103 18-19 0.10004653445371048 1103 20-21 0.07692526176496983 1103 22-23 0.006264559086623933 1103 24-25 0.050859995918777656 1103 26-27 -0.1860053079520938 1103 28-29 -0.023896808414072268 1103 30-31 -0.013923883301067974 1103 32-33 0.07402876930343183 1103 34-35 -0.04524277298627766 1103 36-37 9.638215182476984E-4 1103 38-39 0.1638469074211244 1103 40-41 0.15351747872878008 1103 42-43 0.2839818604133555 1103 44-45 0.05640506562421166 1103 46-47 0.2055749994071192 1103 48-49 0.23513317649596388 1103 50-51 0.0359047985926324 1103 52-53 -0.05622613655797437 1103 54-55 0.07181689744814435 1103 56-57 0.28660916266661474 1103 58-59 0.28984330927784896 1103 60-61 0.2404805579602538 1103 62-63 0.10012670197834694 1103 64-65 0.2693458431182414 1103 66-67 0.3111200102203604 1103 68-69 0.42880671592862285 1103 70-71 0.5475053333396254 1103 72-73 0.4818696357913481 1103 74-75 0.4952945172135479 1103 76-77 0.41804030811819004 1103 78-79 0.358103748335477 1103 80-81 0.5069210043328738 1103 82-83 0.4175033426946797 1103 84-85 0.3705415327117443 1103 86-87 0.4313406334896399 1103 88-89 0.6147382702604247 1103 90-91 0.6251002027608656 1103 92-93 0.6828707816456259 1103 94-95 0.6446624998425321 1103 96-97 0.9454645273334172 1103 98-99 0.9795397010367601 1103 100-101 1.311195859734834 1104 1 -0.1402889604820352 1104 2 -0.06321114274488693 1104 3 0.016920369029257643 1104 4 0.024244770590819087 1104 5 -0.036964227091146995 1104 6 -0.007372683830169535 1104 7 -0.06078600671136769 1104 8 0.17143519677290442 1104 9 0.0732984542030124 1104 10-11 0.0957435197486447 1104 12-13 0.1371629198452382 1104 14-15 0.20469800521390624 1104 16-17 0.15880229817773284 1104 18-19 0.1589961848428345 1104 20-21 0.3094646503163574 1104 22-23 0.20268131218584529 1104 24-25 0.20767670245026437 1104 26-27 0.1194157773586042 1104 28-29 0.06101153779594881 1104 30-31 0.06111509954647687 1104 32-33 0.04385481055117424 1104 34-35 0.09083382389932382 1104 36-37 0.1374621740078581 1104 38-39 0.15565980386433154 1104 40-41 0.21949286132514345 1104 42-43 0.3015020926876346 1104 44-45 0.3236956342446078 1104 46-47 0.33776107282893264 1104 48-49 0.5296076665438676 1104 50-51 0.5394954539983772 1104 52-53 0.4920972482115573 1104 54-55 0.3700623165225778 1104 56-57 0.4799298662629141 1104 58-59 0.5898782350869638 1104 60-61 0.7065732418852093 1104 62-63 0.5382478698409585 1104 64-65 0.5057824580664878 1104 66-67 0.5136165000001505 1104 68-69 0.5097395172607442 1104 70-71 0.6186975381812587 1104 72-73 0.6547077634857494 1104 74-75 0.7219132755481183 1104 76-77 0.7465170136618688 1104 78-79 0.5835563727101665 1104 80-81 0.7639980668959332 1104 82-83 0.7448576597363576 1104 84-85 0.6972312010223938 1104 86-87 0.6718965535906776 1104 88-89 0.7243282271499787 1104 90-91 0.8460085913052033 1104 92-93 0.5972192918305552 1104 94-95 1.0428424771231164 1104 96-97 0.9188163113719732 1104 98-99 1.097260315730999 1104 100-101 0.8636912911567727 1105 1 0.0538576636848731 1105 2 0.0663261894293612 1105 3 0.14780996624745768 1105 4 0.16956013811714143 1105 5 0.21357184940841734 1105 6 0.16644166949737382 1105 7 0.24625060103517882 1105 8 0.16148156014583748 1105 9 0.18219442638498862 1105 10-11 0.183348561765456 1105 12-13 0.22832490187943222 1105 14-15 0.27974871515305466 1105 16-17 0.26344587974829636 1105 18-19 0.2040676617094448 1105 20-21 0.35361610400128285 1105 22-23 0.38362886362020987 1105 24-25 0.312542441849466 1105 26-27 0.3212365056499138 1105 28-29 0.1354996088864553 1105 30-31 0.1812710929783421 1105 32-33 0.17644198335132444 1105 34-35 0.24035570161592545 1105 36-37 0.16808856249428317 1105 38-39 0.24701738137810025 1105 40-41 0.2678121890562366 1105 42-43 0.26392844253791736 1105 44-45 0.455176847419537 1105 46-47 0.46346618245899407 1105 48-49 0.514718262506392 1105 50-51 0.49065212356274657 1105 52-53 0.324413968002915 1105 54-55 0.38185358475400477 1105 56-57 0.5574267270762121 1105 58-59 0.6288234683526852 1105 60-61 0.5938812685441448 1105 62-63 0.5557234807749865 1105 64-65 0.5312847279399513 1105 66-67 0.6576206533856386 1105 68-69 0.7575251832512784 1105 70-71 0.7259490594794329 1105 72-73 0.7558523592550905 1105 74-75 0.9488982074519114 1105 76-77 0.838642484539875 1105 78-79 0.8500636652823665 1105 80-81 0.8871459467375225 1105 82-83 0.8168102338694574 1105 84-85 1.1356983120047168 1105 86-87 0.9546889809064325 1105 88-89 0.7433299657764643 1105 90-91 0.7851687381225467 1105 92-93 0.960976173893723 1105 94-95 1.0032235260944269 1105 96-97 1.0587298629995203 1105 98-99 1.2124105137412364 1105 100-101 1.4961891178736657 1106 1 -0.12813536808060633 1106 2 0.052022971205261115 1106 3 0.03863331816830495 1106 4 -0.042770969715455465 1106 5 -0.06866665425490481 1106 6 -0.09619450697567089 1106 7 -0.13782748320043936 1106 8 -0.08264420148385909 1106 9 -0.044452402484836284 1106 10-11 -0.3378322455828666 1106 12-13 -0.4813795732941486 1106 14-15 -0.23871793984431378 1106 16-17 -0.17648435455442524 1106 18-19 -0.3450881900238656 1106 20-21 -0.33889804501558274 1106 22-23 -0.361228312577758 1106 24-25 -0.25201062425707477 1106 26-27 -0.2963607870742777 1106 28-29 -0.057214585140535235 1106 30-31 0.06807062287256116 1106 32-33 0.011708043214795794 1106 34-35 -0.19445567260994068 1106 36-37 -0.29692335685423643 1106 38-39 -0.41921898013657 1106 40-41 -0.4650558312483284 1106 42-43 -0.3434363395380515 1106 44-45 -0.5043546721723686 1106 46-47 -0.13392343853906397 1106 48-49 0.15207208378284065 1106 50-51 0.18349863902872698 1106 52-53 0.09651789972088665 1106 54-55 0.032230127809533826 1106 56-57 0.024523490514646085 1106 58-59 -0.05945952031976276 1106 60-61 -0.23912803354881618 1106 62-63 0.05404185240860215 1106 64-65 -0.059346080658649214 1106 66-67 0.24511950729443654 1106 68-69 0.15705570261813406 1106 70-71 0.02653960901974628 1106 72-73 0.05238262023047824 1106 74-75 -0.017110811243959745 1106 76-77 0.16823956054864553 1106 78-79 0.15767871200121064 1106 80-81 -0.0332267621219664 1106 82-83 -0.233118708475903 1106 84-85 -0.0726933677898991 1106 86-87 -0.19230242549334164 1106 88-89 0.32197891178931926 1106 90-91 0.5895070058994634 1106 92-93 0.36998799321973763 1106 94-95 0.4486029031209249 1106 96-97 0.3956317075812166 1106 98-99 0.34251582910386347 1106 100-101 0.10749541178979527 1107 1 0.4136845041862536 1107 2 0.45461097028469055 1107 3 0.42629146495972137 1107 4 0.3477307035993604 1107 5 0.44022347582554033 1107 6 0.3417351472823924 1107 7 0.33782305281845737 1107 8 0.3307071839229394 1107 9 0.5109220079173156 1107 10-11 0.5169616274164781 1107 12-13 0.5430696702543969 1107 14-15 0.6863605075397388 1107 16-17 0.6379130342028319 1107 18-19 0.6667497381394369 1107 20-21 0.7673890808205996 1107 22-23 0.8273776254151599 1107 24-25 0.7014057937252716 1107 26-27 0.623296303812289 1107 28-29 0.6162602210921335 1107 30-31 0.5851903690578055 1107 32-33 0.7746456492544738 1107 34-35 0.6108188626504756 1107 36-37 0.6592031659310535 1107 38-39 0.6820310934556346 1107 40-41 0.7622202324362632 1107 42-43 0.7881589560656082 1107 44-45 0.8369320675436427 1107 46-47 0.8571525980788834 1107 48-49 0.7137887090467245 1107 50-51 0.5605847392380028 1107 52-53 0.5662618485297628 1107 54-55 0.6646258581615498 1107 56-57 0.6714834058893402 1107 58-59 0.6968807830559953 1107 60-61 0.8982526610545918 1107 62-63 0.7083725532434642 1107 64-65 0.7544419263725608 1107 66-67 0.789800109458394 1107 68-69 0.9468345857445293 1107 70-71 0.9533861809225819 1107 72-73 0.9076382126649669 1107 74-75 0.9485473363120498 1107 76-77 0.947389721533991 1107 78-79 1.0803257492361915 1107 80-81 1.2255238294829383 1107 82-83 1.3569415262698676 1107 84-85 1.2865194784662215 1107 86-87 1.3347301913385934 1107 88-89 1.5323325902146827 1107 90-91 1.5240545724631396 1107 92-93 1.583855365148267 1107 94-95 1.749825279281481 1107 96-97 1.6196747794767923 1107 98-99 1.8357497422291154 1107 100-101 1.8312393361316524 1108 1 0.12077063577025982 1108 2 0.13570221570194008 1108 3 0.26439453110788236 1108 4 0.22908394764095164 1108 5 0.245181044810721 1108 6 0.17998846752692543 1108 7 0.08204698855570314 1108 8 0.2595932186023262 1108 9 0.21339311275608708 1108 10-11 0.30054889017267783 1108 12-13 0.19404739777434798 1108 14-15 0.2116042159740701 1108 16-17 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20-21 0.06124685464062907 2316 22-23 0.23793228298981717 2316 24-25 0.07854727561840491 2316 26-27 0.22048263441927674 2316 28-29 -0.03895267771910227 2316 30-31 0.05134096984570391 2316 32-33 -0.014274088930385176 2316 34-35 0.1377063626458792 2316 36-37 0.27819330753705884 2316 38-39 0.027730783845576923 2316 40-41 -0.013870777528929068 2316 42-43 0.07773348989199036 2316 44-45 -0.21171063095095377 2316 46-47 -0.06732751517350266 2316 48-49 -0.02406580773582334 2316 50-51 0.09024572965875421 2316 52-53 -0.23322190145319865 2316 54-55 -0.17254638955153467 2316 56-57 -0.10080643803875233 2316 58-59 0.11767849944698838 2316 60-61 0.048010648143552714 2316 62-63 0.40953107750054585 2316 64-65 0.2763184719830569 2316 66-67 0.3409084192128198 2316 68-69 0.40768918612584315 2316 70-71 0.40722492957791445 2316 72-73 0.23338928499410727 2316 74-75 0.3261033134214557 2316 76-77 0.3518691055988903 2316 78-79 0.5381033993447808 2316 80-81 0.6752736040056426 2316 82-83 0.6509108878735752 2316 84-85 0.5348566179373258 2316 86-87 0.4762341810622033 2316 88-89 0.7454768577513669 2316 90-91 0.5652262294607091 2316 92-93 0.7432292361505795 2316 94-95 0.7002613370876389 2316 96-97 0.8347143820917005 2316 98-99 0.9710628196176394 2316 100-101 1.296807691751237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9663.0 3 2314.0 4 2298.0 5 2554.0 6 3012.0 7 3725.0 8 4644.0 9 5701.0 10 7102.0 11 8977.0 12 11606.0 13 13972.0 14 16046.0 15 19238.0 16 23534.0 17 28729.0 18 25070.0 19 19459.0 20 18586.0 21 19729.0 22 21214.0 23 23438.0 24 26010.0 25 29312.0 26 33456.0 27 38628.0 28 45342.0 29 53320.0 30 63857.0 31 77522.0 32 95358.0 33 121125.0 34 158774.0 35 212526.0 36 283205.0 37 336220.0 38 214098.0 39 22123.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.340341055307256 18.426315791986532 14.584251412867708 22.6490917398385 2 15.470602271642644 19.753412236327193 40.7343705259139 24.041614966116253 3 19.421651860169145 24.507477372710014 29.160441288821424 26.91042947829942 4 12.593351560221988 15.950786446181445 36.37672105940569 35.079140934190875 5 13.4842249591599 37.79280670164224 33.43419076467273 15.288777574525131 6 32.4686429519428 35.476229876813214 17.00937146650503 15.045755704738955 7 28.981326950036717 33.12082169049277 19.81531991187999 18.082531447590526 8 27.126326174727705 34.29235473889456 19.148844858836213 19.43247422754152 9 27.07105015043844 16.519328084560282 19.977766872640235 36.431854892361045 10-11 25.24879845179357 26.821374443339096 26.128463363152264 21.801363741715065 12-13 26.31849889936327 24.03241251177891 27.2649368039951 22.384151784862716 14-15 23.218913623566873 25.11655655004534 26.062385562605765 25.602144263782023 16-17 21.98776747473808 28.578287804069802 26.818411011418377 22.615533709773743 18-19 22.02284180513346 26.496694225319107 29.855779008169847 21.624684961377593 20-21 22.750421412699808 25.39408732534109 30.346053071658158 21.50943819030094 22-23 22.47146730675837 25.38447105294465 30.242997864533155 21.90106377576382 24-25 22.054714799898246 25.617692560224352 30.364952087775226 21.962640552102176 26-27 21.401901712055817 26.131935416269812 30.167143052299583 22.299019819374784 28-29 21.752851458980814 26.442253812635645 30.06704153589879 21.73785319248475 30-31 21.723514784262008 25.916861729256386 30.129379132288797 22.23024435419281 32-33 21.519447647003418 25.895063899496744 30.12202842600618 22.463460027493653 34-35 22.216263993459247 26.06186485354242 29.987532227487137 21.7343389255112 36-37 22.268113444053952 25.696485709831112 29.778229776513328 22.257171069601604 38-39 21.806348330060533 26.053196261807 29.811245376243512 22.329210031888962 40-41 22.231346038930067 25.970635717621025 29.73521518607779 22.062803057371116 42-43 21.731548292952027 25.958920421995792 29.653064304222603 22.656466980829578 44-45 21.892396594007497 26.29202211315625 29.68421117878266 22.131370114053592 46-47 22.403647384819457 26.63948388474901 28.725587170260958 22.231281560170572 48-49 22.19634820241665 26.72844002789816 28.569790108075566 22.505421661609628 50-51 22.60975513800088 27.098220193395555 28.20465928070968 22.087365387893882 52-53 22.58605352215462 26.79336899569047 28.32689010095325 22.29368738120166 54-55 22.255591451914334 26.99336247160854 28.43225255651293 22.3187935199642 56-57 22.403279287946205 27.372338718962002 28.01069577605993 22.213686217031864 58-59 22.298074878648993 26.771615067261497 28.32317267931393 22.60713737477558 60-61 22.13146245281607 27.09807918082165 28.529958668329915 22.240499698032373 62-63 22.059900312334346 27.11998367776553 28.75933380113569 22.06078220876443 64-65 21.93008967128474 26.995318368574672 28.705131584780624 22.369460375359964 66-67 21.894853506372787 27.147411260673447 28.346722439713268 22.611012793240498 68-69 22.26470230703408 26.861775789370817 28.627578102840474 22.24594380075463 70-71 22.22688343532253 26.995844713910234 28.27835101429454 22.4989208364727 72-73 22.04643482306639 27.2737131841185 28.032126063527535 22.647725929287574 74-75 22.064093673425067 27.665656931426764 27.866499444911817 22.403749950236353 76-77 22.286963618958264 27.27932146439509 28.014241200318846 22.419473716327797 78-79 22.322607282361346 27.322347446625212 27.97987488790114 22.375170383112298 80-81 22.33734707712433 27.593264957623525 27.633643938433256 22.43574402681889 82-83 22.3744206270014 27.519851024670107 27.6342182834616 22.471510064866898 84-85 22.337870754166385 27.649558177593093 27.611880719984995 22.400690348255527 86-87 22.398198834161235 27.662228497057516 27.480124281777496 22.45944838700375 88-89 22.36640333520349 27.588431656404637 27.40360071177193 22.641564296619944 90-91 22.405236566246867 27.509325047474377 27.466206609539306 22.61923177673945 92-93 22.321436658081538 27.193415237678476 27.683759229254466 22.80138887498552 94-95 22.2425103630796 27.22308078053915 27.75229379664339 22.782115059737862 96-97 22.39233585297911 27.05457436911726 27.5649390968413 22.98815068106233 98-99 22.13558452554856 27.174163860145622 27.60605979701511 23.084191817290712 100-101 22.56590925178398 26.544619426012684 28.012394278383155 22.877077043820176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3043.0 1 1810.5 2 662.0 3 750.5 4 648.5 5 618.5 6 987.5 7 1895.5 8 3044.5 9 3651.0 10 3375.5 11 3096.5 12 3264.5 13 3422.0 14 3600.0 15 3729.5 16 3848.5 17 4023.0 18 4172.0 19 4394.5 20 4745.0 21 5464.0 22 6543.0 23 7884.5 24 9538.5 25 11446.5 26 14101.0 27 17273.5 28 19629.0 29 21672.5 30 24319.0 31 28624.0 32 34911.0 33 37660.0 34 38721.5 35 41660.0 36 46163.5 37 50997.5 38 54095.0 39 56421.0 40 57801.0 41 60851.5 42 62930.5 43 64922.5 44 68938.5 45 72230.5 46 74287.0 47 75885.0 48 75352.5 49 74252.5 50 74117.0 51 71736.0 52 69869.0 53 70609.5 54 71940.5 55 68158.0 56 61967.5 57 57592.5 58 53939.5 59 48070.5 60 38958.0 61 32608.5 62 27817.0 63 20817.5 64 15234.0 65 12113.5 66 8926.0 67 6537.5 68 5023.0 69 3722.0 70 2768.5 71 1899.5 72 1331.0 73 1067.5 74 797.5 75 594.5 76 459.0 77 300.5 78 192.0 79 123.5 80 88.0 81 68.5 82 48.0 83 31.0 84 23.0 85 20.5 86 15.0 87 12.5 88 10.0 89 8.0 90 6.0 91 6.0 92 8.5 93 5.0 94 5.0 95 5.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3326212985173846 2 0.2897469365768748 3 0.28075331348391547 4 0.2878911095894387 5 0.29131725172008993 6 0.2185117314437525 7 0.20694850175280477 8 0.2087091581255005 9 0.21974894943537654 10-11 0.21941585228378546 12-13 0.2151331746204715 14-15 0.2162990146510403 16-17 0.20040552198940845 18-19 0.20583024702960614 20-21 0.19412426141654798 22-23 0.20411717596428058 24-25 0.20311788450950732 26-27 0.2033558110463581 28-29 0.21794070775531063 30-31 0.21472869950782517 32-33 0.2208672041585752 34-35 0.2133011402867205 36-37 0.19690800189770208 38-39 0.18548752812886482 40-41 0.1810383018897553 42-43 0.19840693907986193 44-45 0.1991683039977844 46-47 0.20012001014518754 48-49 0.2177741591795151 50-51 0.2137294080530519 52-53 0.23668931885915176 54-55 0.20211859305473406 56-57 0.18265620234034058 58-59 0.18344135991194815 60-61 0.1704505709998958 62-63 0.17782629364226984 64-65 0.17682700218749658 66-67 0.17613701523062933 68-69 0.17958695001496558 70-71 0.18080037535290455 72-73 0.17858765856019232 74-75 0.1938863348796972 76-77 0.18620130773941715 78-79 0.1908170825543222 80-81 0.18382204237090938 82-83 0.1629082997817262 84-85 0.16262278793750526 86-87 0.1672385627524103 88-89 0.1725443245241826 90-91 0.1798010838981313 92-93 0.17492358989269036 94-95 0.17401946905265744 96-97 0.1795631573612805 98-99 0.19000813232902958 100-101 0.17061711957569134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2101489.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.82010874302071 #Duplication Level Percentage of deduplicated Percentage of total 1 75.21106912571793 37.47023642522115 2 13.186702661802311 13.139259211457436 3 4.721034657263894 7.056073800133703 4 2.1676970474536628 4.319796105042656 5 1.216309784426304 3.029834286265927 6 0.742595409029736 2.2197710417957635 7 0.4698967798344281 1.6387216068542532 8 0.34271044368534037 1.3659097257418027 9 0.2586206486800151 1.159605795638602 >10 1.4870628213916182 13.653508559479805 >50 0.120746924680958 4.132373015185404 >100 0.06809618216235028 6.188024154639463 >500 0.005162894218735923 1.799273212669519 >1k 0.002007792196175078 1.579020146132114 >5k 1.912183043976265E-4 0.7061530517770315 >10k+ 9.560915219881324E-5 0.5424398619653851 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11388 0.541901480331327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8945 0.4256505744260379 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5880 0.2798016073365123 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2930 0.13942495059455462 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2328 0.11077859555772122 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2324 0.1105882543282406 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2205 0.10492560275119213 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2131 0.10140429000580066 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.758530737015516E-5 2 0.0 0.0 0.0 0.0 4.758530737015516E-5 3 0.0 0.0 0.0 0.0 4.758530737015516E-5 4 0.0 0.0 0.0 0.0 4.758530737015516E-5 5 0.0 0.0 0.0 0.0 4.758530737015516E-5 6 0.0 0.0 0.0 0.0 4.758530737015516E-5 7 0.0 0.0 0.0 0.0 4.758530737015516E-5 8 0.0 0.0 0.0 0.0 4.758530737015516E-5 9 0.0 0.0 0.0 0.0 4.758530737015516E-5 10-11 0.0 0.0 0.0 4.758530737015516E-5 4.758530737015516E-5 12-13 0.0 0.0 0.0 4.758530737015516E-5 1.6654857579554306E-4 14-15 0.0 0.0 0.0 4.758530737015516E-5 2.141338831656982E-4 16-17 0.0 0.0 0.0 4.758530737015516E-5 3.330971515910861E-4 18-19 0.0 0.0 0.0 4.758530737015516E-5 3.330971515910861E-4 20-21 0.0 0.0 0.0 1.4275592211046548E-4 4.044751126463189E-4 22-23 0.0 0.0 0.0 2.6171919053585336E-4 4.7585307370155163E-4 24-25 0.0 0.0 0.0 3.0930449790600857E-4 4.7585307370155163E-4 26-27 0.0 0.0 0.0 6.424016494970947E-4 6.186089958120171E-4 28-29 0.0 0.0 0.0 0.0012610106453091118 6.186089958120171E-4 30-31 0.0 0.0 0.0 0.0028789110958943875 6.424016494970947E-4 32-33 0.0 0.0 0.0 0.005829200152844007 7.137796105523274E-4 34-35 0.0 0.0 0.0 0.00816088021398161 7.613649179224825E-4 36-37 0.0 0.0 0.0 0.009778780664566885 7.613649179224825E-4 38-39 0.0 0.0 0.0 0.01180115622779848 8.327428789777153E-4 40-41 0.0 0.0 0.0 0.014251799557361472 8.56535532662793E-4 42-43 0.0 0.0 0.0 0.01758277107327233 8.56535532662793E-4 44-45 0.0 0.0 0.0 0.02024754828600102 9.041208400329482E-4 46-47 0.0 0.0 0.0 0.02495849371564638 9.517061474031033E-4 48-49 0.0 0.0 0.0 0.030145292218993296 9.517061474031033E-4 50-51 0.0 0.0 0.0 0.03792548997401367 9.517061474031033E-4 52-53 0.0 0.0 0.0 0.05626962596520847 0.0010944620695135688 54-55 0.0 0.0 0.0 0.07799231877968431 0.0010944620695135688 56-57 0.0 0.0 0.0 0.10147566796685589 0.0011420473768837239 58-59 0.0 4.758530737015516E-5 0.0 0.1362605276544393 0.0011420473768837239 60-61 0.0 4.758530737015516E-5 0.0 0.1856064913972902 0.0012134253379389565 62-63 0.0 4.758530737015516E-5 0.0 0.22374611525446958 0.0012848032989941894 64-65 0.0 4.758530737015516E-5 0.0 0.2605533505052846 0.0013323886063643445 66-67 0.0 4.758530737015516E-5 0.0 0.3271727808235018 0.0013561812600494222 68-69 0.0 4.758530737015516E-5 0.0 0.4115415307907869 0.0013799739137344998 70-71 0.0 4.758530737015516E-5 0.0 0.48960522753152647 0.0013799739137344998 72-73 0.0 4.758530737015516E-5 0.0 0.5611259445088697 0.0014989371821598875 74-75 0.0 4.758530737015516E-5 0.0 0.670405602884431 0.0016179004505852755 76-77 0.0 4.758530737015516E-5 0.0 0.7646958894383935 0.0016179004505852755 78-79 0.0 4.758530737015516E-5 0.0 0.8520625137699983 0.0016179004505852755 80-81 0.0 4.758530737015516E-5 0.0 0.9594625525044385 0.001641693104270353 82-83 0.0 4.758530737015516E-5 0.0 1.1260587136073517 0.0019747902558614394 84-85 0.0 4.758530737015516E-5 0.0 1.2921314363291931 0.002046168216916672 86-87 0.0 4.758530737015516E-5 0.0 1.4847091752562112 0.0021175461779719047 88-89 0.0 4.758530737015516E-5 0.0 1.7250387701291798 0.0021889241390271375 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4930 0.0 61.34513 1 GTATCAA 9490 0.0 47.752552 1 ATCAACG 12505 0.0 35.87987 3 TATCAAC 12670 0.0 35.413456 2 TCAACGC 12740 0.0 35.22308 4 CAACGCA 12945 0.0 34.626904 5 AACGCAG 13305 0.0 33.74292 6 TGGTATC 1675 0.0 30.930334 2 CGCAGAG 15335 0.0 28.997313 8 ACGCAGA 15505 0.0 28.771301 7 GTACATG 11255 0.0 28.64535 1 TACATGG 11035 0.0 28.5571 2 GGACCGA 735 0.0 28.440096 6 ACATGGG 11250 0.0 26.57423 3 GCAGAGT 16395 0.0 26.253214 9 GTGGTAT 2085 0.0 25.999723 1 ACCGAGT 875 0.0 25.51852 8 TAGCGCG 150 1.0537824E-6 25.352657 4 GAGTACT 10115 0.0 23.458155 12-13 CATGGGA 5945 0.0 22.948526 4 >>END_MODULE