FastQCFastQC Report
Thu 26 May 2016
SRR937902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101489
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133280.634216976629428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112150.5336692221562901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76850.36569308713964244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.13756912360711857No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25470.1211997778717852No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24530.11672675897899062No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22570.1074000387344402No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21640.10297460514901577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.102736678612165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54250.059.999531
GTATCAA98750.046.7239071
TATCAAC134050.034.0234832
ATCAACG134400.033.7934843
TGGTATC18250.033.321262
TCAACGC136550.033.296194
CAACGCA140100.032.4193575
AACGCAG144000.031.6657716
TAAGGTG10900.029.2033715
CGCAGAG169650.026.7107548
ACGCAGA170750.026.6221357
GTAAGGT10950.026.4661054
GGTAAGG11100.025.252443
GTGGTAT25600.025.243871
GTACATG108850.024.795731
TACATGG107850.024.4041652
GCAGAGT185550.024.1914839
GAGTACT119450.022.98386212-13
GGACCGA9000.022.6938046
ACATGGG108700.022.6399023
Produced by FastQC (version 0.11.5)