##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937902_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101489 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06452377338164 31.0 31.0 34.0 30.0 34.0 2 32.27233118993247 34.0 31.0 34.0 30.0 34.0 3 32.35240822102804 34.0 31.0 34.0 30.0 34.0 4 36.02237603908467 37.0 35.0 37.0 35.0 37.0 5 35.91999339515934 37.0 35.0 37.0 35.0 37.0 6 35.95609018177112 37.0 35.0 37.0 35.0 37.0 7 35.89260757491474 37.0 35.0 37.0 35.0 37.0 8 35.86540543395659 37.0 35.0 37.0 35.0 37.0 9 37.52089637395199 39.0 37.0 39.0 35.0 39.0 10-11 37.442198365064016 39.0 37.0 39.0 34.5 39.0 12-13 37.33004098522524 39.0 37.0 39.0 34.0 39.0 14-15 38.48539535538849 40.0 38.0 41.0 34.0 41.0 16-17 38.445204328930586 40.0 38.0 41.0 34.0 41.0 18-19 38.4493611434559 40.0 38.0 41.0 34.0 41.0 20-21 38.33946930010103 40.0 38.0 41.0 34.0 41.0 22-23 38.180345221887904 40.0 37.5 41.0 33.5 41.0 24-25 38.23860891015846 40.0 38.0 41.0 33.5 41.0 26-27 38.08888554734286 40.0 37.0 41.0 33.0 41.0 28-29 37.931180225068985 40.0 37.0 41.0 33.0 41.0 30-31 37.92982689892738 40.0 37.0 41.0 33.0 41.0 32-33 37.81316961449715 40.0 37.0 41.0 33.0 41.0 34-35 37.663827647920115 40.0 37.0 41.0 33.0 41.0 36-37 37.462861808936424 40.0 36.0 41.0 32.5 41.0 38-39 37.281063093834895 40.0 36.0 41.0 32.0 41.0 40-41 37.06659230669302 39.5 36.0 41.0 31.0 41.0 42-43 36.80888265415617 39.0 35.5 41.0 31.0 41.0 44-45 36.45378205643713 39.0 35.0 41.0 30.0 41.0 46-47 36.03848556904176 39.0 35.0 41.0 29.5 41.0 48-49 35.64111970131654 39.0 35.0 40.0 28.0 41.0 50-51 35.336583964988634 39.0 35.0 40.0 27.0 41.0 52-53 35.04618344421503 38.0 34.5 40.0 26.0 41.0 54-55 34.794821433754834 38.0 34.0 40.0 26.0 41.0 56-57 34.492483424847805 38.0 34.0 40.0 25.0 41.0 58-59 34.19125605701481 38.0 34.0 40.0 24.5 41.0 60-61 33.80978796462889 37.0 33.0 40.0 23.0 41.0 62-63 33.42581022313226 36.5 33.0 39.5 22.5 41.0 64-65 33.01194652934181 36.0 32.0 39.0 22.0 40.5 66-67 32.55517397426301 35.5 32.0 39.0 20.0 40.0 68-69 32.090931239706705 35.0 31.0 38.0 18.5 40.0 70-71 31.652077883824276 35.0 31.0 37.5 18.0 40.0 72-73 31.1477457174413 35.0 30.5 37.0 14.5 39.0 74-75 30.639734017165924 34.0 30.0 36.5 11.0 39.0 76-77 29.445003043080405 33.5 28.5 35.0 9.0 37.5 78-79 30.052945554318867 34.0 30.0 35.0 7.5 37.5 80-81 29.86727006422589 34.0 30.0 35.0 7.0 37.0 82-83 29.49564523059602 34.0 29.0 35.0 2.0 37.0 84-85 29.10192630082765 34.0 29.0 35.0 2.0 36.0 86-87 28.66435465519924 34.0 29.0 35.0 2.0 36.0 88-89 28.258716557640795 33.5 28.5 35.0 2.0 35.0 90-91 27.694843751263985 33.0 27.0 35.0 2.0 35.0 92-93 27.17239704799787 33.0 26.0 35.0 2.0 35.0 94-95 26.65307907869135 33.0 25.0 34.5 2.0 35.0 96-97 26.044106821401396 32.0 24.5 34.0 2.0 35.0 98-99 25.086785607728615 32.0 20.5 34.0 2.0 35.0 100-101 22.438005623631625 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38406677906304765 1101 2 0.2929200801300311 1101 3 0.2652266467089106 1101 4 0.16323787502691545 1101 5 0.17807058438634016 1101 6 0.18717169966033964 1101 7 0.2600769784787289 1101 8 0.20351003147895597 1101 9 0.31044252084244306 1101 10-11 0.32911786447533586 1101 12-13 0.2989123471440749 1101 14-15 0.3535057504079617 1101 16-17 0.33435817225202413 1101 18-19 0.4376325750061838 1101 20-21 0.4216784403000844 1101 22-23 0.4048352309804599 1101 24-25 0.40398913520083113 1101 26-27 0.35341882258898494 1101 28-29 0.37621892333501705 1101 30-31 0.4691438447976566 1101 32-33 0.5347572806970149 1101 34-35 0.44898897079458067 1101 36-37 0.43926941719981016 1101 38-39 0.3803907150430561 1101 40-41 0.341400293675278 1101 42-43 0.49156475981285297 1101 44-45 0.5175528675566383 1101 46-47 0.4942044596976771 1101 48-49 0.5466845528412136 1101 50-51 0.5251183624796454 1101 52-53 0.5650775351457398 1101 54-55 0.5949569407124926 1101 56-57 0.5936193829228102 1101 58-59 0.5267345745913374 1101 60-61 0.5679664043649737 1101 62-63 0.5969181591103734 1101 64-65 0.7050688519790071 1101 66-67 0.6201799647497452 1101 68-69 0.5623282307088147 1101 70-71 0.5027114409282092 1101 72-73 0.4753620739519029 1101 74-75 0.5456535638391706 1101 76-77 0.4546754990007962 1101 78-79 0.38929652529362 1101 80-81 0.42714589952805326 1101 82-83 0.3855848179008312 1101 84-85 0.428415028434447 1101 86-87 0.3382937782568902 1101 88-89 0.5457957482265172 1101 90-91 0.5367900523274187 1101 92-93 0.6289637121143521 1101 94-95 0.7061435237978237 1101 96-97 0.8554904347836896 1101 98-99 1.030623184313594 1101 100-101 1.327443429406273 1102 1 0.2521084261987383 1102 2 0.27153736021319475 1102 3 0.28461173683163565 1102 4 0.1641404267028932 1102 5 0.11226646689261344 1102 6 0.2059304550066443 1102 7 0.20894729166914772 1102 8 0.18378653246539045 1102 9 0.33146840461174776 1102 10-11 0.29470860969279045 1102 12-13 0.3160841509843948 1102 14-15 0.36183035958696763 1102 16-17 0.4004659555797403 1102 18-19 0.332524174749274 1102 20-21 0.33098754486209714 1102 22-23 0.43889484776261867 1102 24-25 0.27634506380595525 1102 26-27 0.2316140801041442 1102 28-29 0.29728951239683 1102 30-31 0.33760269654154484 1102 32-33 0.2804137267625464 1102 34-35 0.4083909410522253 1102 36-37 0.44158875618339977 1102 38-39 0.4957233634966798 1102 40-41 0.34894567943263155 1102 42-43 0.4316558288509498 1102 44-45 0.3859901248591058 1102 46-47 0.4155187300339733 1102 48-49 0.4402410402529 1102 50-51 0.3666942461145908 1102 52-53 0.3657804198176038 1102 54-55 0.3810887600931281 1102 56-57 0.24251296052479177 1102 58-59 0.38422335411957675 1102 60-61 0.30510859406820856 1102 62-63 0.43531937193098713 1102 64-65 0.4710933671406039 1102 66-67 0.38333241920275185 1102 68-69 0.33392487955863714 1102 70-71 0.302323374419629 1102 72-73 0.472516886325959 1102 74-75 0.44515620066442807 1102 76-77 0.37856181127292743 1102 78-79 0.6872648818548974 1102 80-81 0.6415364736791425 1102 82-83 0.7077727321541971 1102 84-85 0.6609864906794947 1102 86-87 0.5904468880292093 1102 88-89 0.7419493847112548 1102 90-91 0.8348159940658064 1102 92-93 0.633068191588503 1102 94-95 0.8365443895717668 1102 96-97 1.064938953059496 1102 98-99 1.0444306701562773 1102 100-101 1.2012195044543468 1103 1 0.14020734009597646 1103 2 0.13584039730407937 1103 3 0.1471326635436725 1103 4 0.03772807952187662 1103 5 0.017939060750791214 1103 6 0.06864902797141781 1103 7 0.03090608315747545 1103 8 0.1178627228997513 1103 9 0.14017830895828354 1103 10-11 0.10466697953577864 1103 12-13 0.10618108549100924 1103 14-15 0.13216687795305404 1103 16-17 0.10189530399860303 1103 18-19 -0.0057320143235273235 1103 20-21 -0.05723013979257985 1103 22-23 0.05508778564240657 1103 24-25 0.011215060153162426 1103 26-27 -0.0730610955997193 1103 28-29 -0.11122516137339744 1103 30-31 -0.04985459905746126 1103 32-33 0.11157404935050863 1103 34-35 0.10440935729084089 1103 36-37 0.015093214570448765 1103 38-39 0.01425910489339799 1103 40-41 0.052208013839837975 1103 42-43 0.23252320309120478 1103 44-45 0.3255996783469399 1103 46-47 0.3310959632353203 1103 48-49 0.21974633168229474 1103 50-51 0.1427731201458471 1103 52-53 0.13999043872576777 1103 54-55 0.19295441627502896 1103 56-57 0.22189790734697112 1103 58-59 0.2929667851267581 1103 60-61 0.3695665272593871 1103 62-63 0.370846798276645 1103 64-65 0.5243910582663602 1103 66-67 0.37019022443627136 1103 68-69 0.31641214052562106 1103 70-71 0.2922176117137205 1103 72-73 0.4154565961666208 1103 74-75 0.30048060770737095 1103 76-77 0.1560018823670184 1103 78-79 0.36806618368487776 1103 80-81 0.154323088196481 1103 82-83 0.24324913648572632 1103 84-85 0.2916301147211833 1103 86-87 0.4840564722620151 1103 88-89 0.3111625403386462 1103 90-91 0.42527502382767324 1103 92-93 0.3664995794238841 1103 94-95 0.2190116663065318 1103 96-97 0.20311052025519416 1103 98-99 0.37607376201713194 1103 100-101 0.8324191000847527 1104 1 0.06724803042752825 1104 2 0.14917394230898395 1104 3 -0.018438602746222443 1104 4 0.01927878544656636 1104 5 -0.0703593820520183 1104 6 -0.0019303876739300563 1104 7 0.011506030945156454 1104 8 0.013214415213951725 1104 9 0.06551080971677692 1104 10-11 0.11225822762192195 1104 12-13 0.07649256020684447 1104 14-15 0.01922616010421052 1104 16-17 0.08216574970837343 1104 18-19 0.05051282618195074 1104 20-21 5.532002436865469E-4 1104 22-23 -0.012172972565160478 1104 24-25 -0.13376003315872254 1104 26-27 -0.1228469545276738 1104 28-29 -0.12902374176272957 1104 30-31 0.047087981501206855 1104 32-33 -0.016239947784868036 1104 34-35 -0.10630651596022034 1104 36-37 -0.04629065887817774 1104 38-39 -0.1201853277915177 1104 40-41 -0.009463909337497967 1104 42-43 -0.09182925829637156 1104 44-45 -0.040151508777782396 1104 46-47 -0.027864447125288905 1104 48-49 -0.05581406880030926 1104 50-51 -0.01986367260651889 1104 52-53 0.1067299556912431 1104 54-55 0.04629265333307586 1104 56-57 0.09180614772120066 1104 58-59 0.08999068284705203 1104 60-61 0.0024650519085369638 1104 62-63 -0.06757088617749218 1104 64-65 -0.030839699023957223 1104 66-67 0.04259901162873092 1104 68-69 0.03522109987762434 1104 70-71 0.02598081472792302 1104 72-73 0.02957420794684751 1104 74-75 -0.060922316182587366 1104 76-77 -0.09853829642490197 1104 78-79 0.14320218266885476 1104 80-81 0.12007621564393389 1104 82-83 0.21759612969106712 1104 84-85 0.21443190546267132 1104 86-87 0.2108618543644809 1104 88-89 0.06647408561166657 1104 90-91 0.09199090413445532 1104 92-93 0.18355466342222115 1104 94-95 0.16533634227263505 1104 96-97 0.41467502011055046 1104 98-99 0.5934841014677694 1104 100-101 0.732452791143043 1105 1 0.11654775031548326 1105 2 0.11457044573793951 1105 3 0.16749784079671315 1105 4 -0.02895844047828433 1105 5 -0.045666056445917036 1105 6 -0.052683792484131686 1105 7 0.03404539151696895 1105 8 0.050416192476582466 1105 9 0.10000322737349165 1105 10-11 0.10720655177916427 1105 12-13 0.09439316872611414 1105 14-15 0.11577546948312545 1105 16-17 0.047224186876348995 1105 18-19 0.1575301158564386 1105 20-21 0.2070730495627231 1105 22-23 0.07506985560058865 1105 24-25 0.0627747876661573 1105 26-27 -0.07481015360040288 1105 28-29 -0.022868521053752033 1105 30-31 0.02997699917725072 1105 32-33 -0.012675763490456404 1105 34-35 0.08679934707465264 1105 36-37 0.07668094355589261 1105 38-39 0.1209500918936044 1105 40-41 0.1821496326310168 1105 42-43 0.21810988977665602 1105 44-45 0.18855997581605521 1105 46-47 0.07054422668905858 1105 48-49 0.11035032799288302 1105 50-51 0.19102447575627224 1105 52-53 0.10748577526045011 1105 54-55 0.1588759625188203 1105 56-57 0.0633168209560182 1105 58-59 5.794010929704996E-4 1105 60-61 0.2521366446835813 1105 62-63 0.1320210195503222 1105 64-65 0.1773584078049737 1105 66-67 0.16904545227395573 1105 68-69 0.03843031459799562 1105 70-71 0.243521624155143 1105 72-73 0.16619644304778447 1105 74-75 0.06577284464036026 1105 76-77 -0.06536433718603618 1105 78-79 0.2396838787955815 1105 80-81 0.07846267367542126 1105 82-83 0.30604150784232687 1105 84-85 0.3272446167540828 1105 86-87 0.4091328869154296 1105 88-89 0.38861742570632885 1105 90-91 0.29754002033266147 1105 92-93 0.49397917805566394 1105 94-95 0.3802088429970638 1105 96-97 0.4557235084714151 1105 98-99 0.6312919832411694 1105 100-101 0.9782873318558778 1106 1 -0.13296880757670948 1106 2 0.015960758558321686 1106 3 -0.036258421028861676 1106 4 -0.021148349874565042 1106 5 0.004253508789595628 1106 6 0.004431141709396513 1106 7 -0.05096748638055715 1106 8 -0.044390555708424984 1106 9 -0.11971079161411069 1106 10-11 -0.18737847985295275 1106 12-13 -0.30114895325132807 1106 14-15 -0.11431796820701834 1106 16-17 0.023592828153965684 1106 18-19 0.003686125958715536 1106 20-21 -0.08738278633368424 1106 22-23 -0.10067593720278722 1106 24-25 -0.051122714271770064 1106 26-27 -0.0537772962074925 1106 28-29 -0.04872329671161424 1106 30-31 6.995784242462832E-4 1106 32-33 -0.029828581207780758 1106 34-35 -0.06206018798740587 1106 36-37 -0.08234442048433976 1106 38-39 -0.3257577090282737 1106 40-41 -0.25511541867219023 1106 42-43 -0.2975655121887968 1106 44-45 -0.2771458496613448 1106 46-47 -0.02653178207958007 1106 48-49 -0.03152430213221891 1106 50-51 -0.02481575496230448 1106 52-53 -0.10279278741615627 1106 54-55 -0.02552907028022844 1106 56-57 -0.1400164290252306 1106 58-59 -0.3059114343449991 1106 60-61 -0.27629371047967055 1106 62-63 -0.15174491111741162 1106 64-65 -0.1238927070291922 1106 66-67 -0.1353105278215665 1106 68-69 -0.20309381999895493 1106 70-71 -0.2066369531914276 1106 72-73 -0.24067739523249543 1106 74-75 -0.2789401324461487 1106 76-77 -0.31766589670359124 1106 78-79 -0.27304502687485765 1106 80-81 -0.21203393806221982 1106 82-83 -0.2977776848093505 1106 84-85 -0.30844841417787805 1106 86-87 -0.21087953254561498 1106 88-89 -0.5138064529997166 1106 90-91 -0.39301848032998876 1106 92-93 -0.3452940334169199 1106 94-95 0.009948451075548093 1106 96-97 0.03611034550974779 1106 98-99 0.06534172776702363 1106 100-101 0.16055855121356544 1107 1 0.23562226670134123 1107 2 0.34370720070949545 1107 3 0.28064492583014555 1107 4 0.16003375892262994 1107 5 0.1468591547074638 1107 6 0.2113393666805905 1107 7 0.14453260804685186 1107 8 0.07659694852551979 1107 9 0.21705098294829384 1107 10-11 0.2633546897042649 1107 12-13 0.28629436163234345 1107 14-15 0.3298588044859514 1107 16-17 0.316169744437758 1107 18-19 0.32544732325235515 1107 20-21 0.35998970417196574 1107 22-23 0.28348313895310184 1107 24-25 0.3895742937939275 1107 26-27 0.44628635627518776 1107 28-29 0.4484348015280659 1107 30-31 0.3207384312879924 1107 32-33 0.4589879834237607 1107 34-35 0.3976451143982018 1107 36-37 0.269087435423252 1107 38-39 0.28566902027396424 1107 40-41 0.30171525731334725 1107 42-43 0.3380254581044255 1107 44-45 0.23851371239428687 1107 46-47 0.1248021866316904 1107 48-49 0.2594200139877998 1107 50-51 0.4593635447151172 1107 52-53 0.464090871137401 1107 54-55 0.3966836773432192 1107 56-57 0.2161347901587476 1107 58-59 0.09300115951851495 1107 60-61 0.34142477045478614 1107 62-63 0.369409599645401 1107 64-65 0.3932534176623932 1107 66-67 0.40312343377897975 1107 68-69 0.42019425316966874 1107 70-71 0.5293923781784287 1107 72-73 0.5049021023219531 1107 74-75 0.5846007959090649 1107 76-77 0.35095201483742144 1107 78-79 0.5624180841220721 1107 80-81 0.5534922978472601 1107 82-83 0.4654243753137628 1107 84-85 0.5735632986435313 1107 86-87 0.5631709750303706 1107 88-89 0.6511782971652451 1107 90-91 0.7296043001480825 1107 92-93 0.952219390949022 1107 94-95 1.11942087712589 1107 96-97 1.0055219824833905 1107 98-99 1.2066853150756742 1107 100-101 1.355024436158935 1108 1 0.13830472896901114 1108 2 0.026311004030219465 1108 3 0.015198990222003772 1108 4 -0.0010438263567777994 1108 5 -0.04402402032111752 1108 6 -0.042831575735363 1108 7 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0.039356977104969104 2316 80-81 -0.021605175739026095 2316 82-83 0.09408435334238519 2316 84-85 0.1405879669092549 2316 86-87 0.1555376709013636 2316 88-89 0.19478389364245885 2316 90-91 0.24751763805540605 2316 92-93 0.21459265319025533 2316 94-95 0.44329926338240355 2316 96-97 0.7461641683370814 2316 98-99 0.9477046160794842 2316 100-101 1.598153961586771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 25.0 5 46.0 6 145.0 7 294.0 8 788.0 9 1468.0 10 2347.0 11 3875.0 12 5844.0 13 8044.0 14 10464.0 15 14573.0 16 19500.0 17 26472.0 18 23692.0 19 15636.0 20 13187.0 21 13209.0 22 13757.0 23 15034.0 24 17419.0 25 20613.0 26 24524.0 27 30384.0 28 37327.0 29 46638.0 30 57175.0 31 70144.0 32 88983.0 33 114223.0 34 155335.0 35 217475.0 36 314309.0 37 421445.0 38 276571.0 39 20517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2762204295676 19.3065063399464 15.28436114824875 22.13291208223725 2 15.984285428094081 20.53524905436098 39.71798091734004 23.762484600204903 3 19.672670187662177 24.539076816485835 29.746384587309283 26.041868408542708 4 13.169813655138283 16.739964215695604 35.80657441422236 34.283647714943754 5 13.338480189284988 38.04624459592024 33.5687655018494 15.046509712945374 6 32.560674835795 36.09435976110272 16.484549764476522 14.860415638625755 7 28.90812181267663 33.412213911183926 19.61804225480124 18.061622021338202 8 27.307066560900388 34.70596324796371 18.74099745466191 19.245972736473995 9 26.990100828507785 16.455475141673357 19.92649021717458 36.62793381264427 10-11 25.404707187310326 27.122251680178124 25.81451195188553 21.65852918062602 12-13 26.465137427605345 24.214916088703067 27.246610677421955 22.073335806269633 14-15 23.172831263927623 25.26513343633966 26.16944461760209 25.392590682130624 16-17 21.89913913420437 28.752541650229908 26.956362845582348 22.39195636998338 18-19 21.895118175731586 26.493167463641253 30.289095017865904 21.32261934276125 20-21 22.50877829957711 25.269796796461936 31.02217047055683 21.199254433404125 22-23 22.377145521944165 25.25294198822729 30.87158036993152 21.49833211989702 24-25 22.06887592559371 25.584050166334443 30.886314418015036 21.460759490056812 26-27 21.35925286869527 26.068652634003307 30.775982427605715 21.79611206969571 28-29 21.658907778921428 26.29482536197436 30.65103278147917 21.39523407762504 30-31 21.596663127905973 25.847744147126157 30.758690623648278 21.79690210131959 32-33 21.44448531493622 25.768038757281147 30.616125042767294 22.17135088501534 34-35 22.088528657537584 25.99680512246317 30.49085672111536 21.423809498883887 36-37 22.328073094838945 25.5983019658918 30.22911373792582 21.844511201343426 38-39 21.762508279847424 26.0044787312609 30.314186899948986 21.91882608894269 40-41 22.208871814113465 25.851598079539826 30.186504672550313 21.753025433796395 42-43 21.848116995407096 25.90084650194784 30.072580766061304 22.178455736583764 44-45 21.970546115289235 26.21698857738217 30.10754044097791 21.704924866350684 46-47 22.49374134244814 26.56642504433761 29.12525357020665 21.8145800430076 48-49 22.32800171687789 26.675752288020544 28.90103160187848 22.09521439322309 50-51 22.713204780039295 27.069449328547517 28.48508843015595 21.73225746125723 52-53 22.803926168540496 26.84049262213602 28.4875628661392 21.868018343184286 54-55 22.448083240026477 27.115511906081828 28.51399650438332 21.92240834950837 56-57 22.552532989703966 27.398692070241626 28.253371775916982 21.79540316413743 58-59 22.467807349931405 26.823338118829078 28.441666837180684 22.267187694058833 60-61 22.267473205903052 27.091267191976737 28.73224651663654 21.909013085483675 62-63 22.158383888756973 27.147179928136666 28.94373941524319 21.750696767863168 64-65 22.104136638354994 27.04677492958564 28.798580435110534 22.050507996948827 66-67 22.08410322395216 27.20561468558722 28.500125387284918 22.210156703175702 68-69 22.41304732646582 26.887376944336584 28.8035192206665 21.896056508531096 70-71 22.412560808074655 26.93861352593328 28.367029282570595 22.281796383421472 72-73 22.29956949572422 27.22529121018478 28.14416349550247 22.330975798588526 74-75 22.23118940903331 27.690390004420674 27.854154839735063 22.22426574681095 76-77 22.43661518095027 27.157434561874936 28.227152271556026 22.17879798561877 78-79 22.47344620885477 27.322341444566213 28.066432895913323 22.137779450665697 80-81 22.48267001350234 27.65631487220362 27.758694734087165 22.102320380206876 82-83 22.553261552583688 27.608805462716017 27.744426396021215 22.09350658867908 84-85 22.458804559556103 27.66752328660349 27.721009490232223 22.152662663608183 86-87 22.51075308983297 27.755439119595675 27.485249744347932 22.248558046223415 88-89 22.39538251211403 27.71718053247007 27.555485658026285 22.33195129738961 90-91 22.534907294601386 27.5855906875809 27.525651031752073 22.353850986065634 92-93 22.332210529421438 27.282493324206243 27.823361697277882 22.56193444909444 94-95 22.24867700949184 27.365882000809904 27.76697855663294 22.618462433065318 96-97 22.456743765967847 27.21418004091385 27.64477948730638 22.684296705811928 98-99 22.06575953521826 27.415065577043002 27.540145313292975 22.97902957444576 100-101 22.131513662405037 27.19756795507142 27.98676891408718 22.684149468436367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 965.0 1 699.5 2 583.0 3 709.5 4 560.0 5 521.0 6 1036.0 7 2304.0 8 3641.0 9 4206.5 10 3823.5 11 3306.0 12 3383.0 13 3661.0 14 3912.0 15 4095.0 16 4203.0 17 4377.5 18 4611.0 19 4879.0 20 5237.0 21 5889.0 22 6837.0 23 8092.0 24 9643.5 25 11656.0 26 14259.0 27 17101.0 28 19550.0 29 21937.5 30 24889.5 31 28935.0 32 34897.0 33 37749.0 34 38725.0 35 41555.5 36 46113.5 37 51176.0 38 54564.0 39 56913.0 40 58336.0 41 61185.0 42 63107.5 43 65558.5 44 69696.5 45 72721.5 46 74656.5 47 76783.5 48 76215.0 49 74535.0 50 74853.0 51 72359.5 52 69721.5 53 70645.5 54 72907.0 55 69031.0 56 62040.0 57 56770.0 58 52848.0 59 47141.0 60 37604.0 61 31035.0 62 26606.0 63 19682.5 64 13770.0 65 10663.5 66 7772.5 67 5929.5 68 4669.0 69 3385.0 70 2448.5 71 1641.5 72 1115.0 73 901.5 74 737.0 75 580.5 76 414.5 77 241.0 78 165.0 79 121.5 80 65.0 81 41.5 82 29.5 83 16.0 84 9.5 85 6.5 86 3.5 87 2.5 88 2.0 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021223047087089202 2 0.0 3 0.0 4 4.282677663313964E-4 5 0.025220212906182236 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.379265368507758E-5 12-13 0.0034975200917064042 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.041208400329482E-4 24-25 0.0 26-27 5.234383810717067E-4 28-29 0.0013323886063643445 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.758530737015516E-5 40-41 6.424016494970947E-4 42-43 0.014870408553173488 44-45 2.379265368507758E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.758530737015516E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.137796105523274E-5 82-83 0.0020699608706017496 84-85 5.948163421269395E-4 86-87 0.0 88-89 0.0 90-91 0.009945329240362429 92-93 0.002545813944303301 94-95 0.0 96-97 0.0 98-99 0.0014037665674195773 100-101 0.01791586822486342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2101489.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.18316778874501 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01644392121072 34.451471248719756 2 14.287568797280054 13.482655117106049 3 5.168140232556471 7.315476832454269 4 2.3809231827159043 4.493579920887892 5 1.3058078237262434 3.0806074823365655 6 0.7776966881880337 2.201651599651638 7 0.5436019474881184 1.7954203329034308 8 0.37987662053549753 1.4339025860598236 9 0.2884894546132469 1.225066170807033 >10 1.6346869579768923 14.297045484703933 >50 0.1334423378164941 4.325051341185109 >100 0.07494142583278936 6.605587276691526 >500 0.005654387510087217 1.8824551647885461 >1k 0.0024233089328945206 1.874641973002923 >5k 1.009712055372717E-4 0.3661242614177606 >10k+ 2.019424110745434E-4 1.1692632072838125 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13328 0.634216976629428 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11215 0.5336692221562901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7685 0.36569308713964244 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.13756912360711857 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2547 0.1211997778717852 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2453 0.11672675897899062 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2257 0.1074000387344402 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2164 0.10297460514901577 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2159 0.102736678612165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.758530737015516E-5 7 0.0 0.0 0.0 0.0 4.758530737015516E-5 8 0.0 0.0 0.0 0.0 4.758530737015516E-5 9 0.0 0.0 0.0 0.0 4.758530737015516E-5 10-11 0.0 0.0 0.0 0.0 4.758530737015516E-5 12-13 0.0 0.0 0.0 0.0 7.137796105523274E-5 14-15 0.0 0.0 0.0 0.0 9.517061474031032E-5 16-17 0.0 0.0 0.0 0.0 1.4275592211046548E-4 18-19 0.0 0.0 0.0 0.0 1.4275592211046548E-4 20-21 0.0 0.0 0.0 7.137796105523274E-5 2.141338831656982E-4 22-23 0.0 0.0 0.0 1.6654857579554306E-4 2.3792653685077581E-4 24-25 0.0 0.0 0.0 2.3792653685077581E-4 2.8551184422093097E-4 26-27 0.0 0.0 0.0 5.472310347567844E-4 2.8551184422093097E-4 28-29 0.0 0.0 0.0 0.0011658400305688014 2.8551184422093097E-4 30-31 0.0 0.0 0.0 0.0028789110958943875 2.8551184422093097E-4 32-33 0.0 0.0 0.0 0.00580540749915893 3.0930449790600857E-4 34-35 0.0 0.0 0.0 0.008184672867666687 5.234383810717067E-4 36-37 0.0 0.0 0.0 0.009897743932992274 5.234383810717067E-4 38-39 0.0 1.4275592211046548E-4 0.0 0.011943912149908946 5.234383810717067E-4 40-41 0.0 1.4275592211046548E-4 0.0 0.014275592211046548 5.234383810717067E-4 42-43 0.0 1.4275592211046548E-4 0.0 0.01758277107327233 5.234383810717067E-4 44-45 0.0 1.4275592211046548E-4 0.0 0.0202713409396861 5.234383810717067E-4 46-47 0.0 1.4275592211046548E-4 0.0 0.024910908408276228 5.234383810717067E-4 48-49 0.0 1.4275592211046548E-4 0.0 0.03031184079478884 5.710236884418619E-4 50-51 0.0 1.4275592211046548E-4 0.0 0.038258587125604746 6.186089958120171E-4 52-53 0.0 1.4275592211046548E-4 0.0 0.056721686385224956 7.851575716075602E-4 54-55 0.0 1.4275592211046548E-4 0.0 0.07856334246812617 8.089502252926377E-4 56-57 0.0 1.4275592211046548E-4 0.0 0.10245116676794405 8.089502252926377E-4 58-59 0.0 1.4275592211046548E-4 0.0 0.13730740441658273 8.089502252926377E-4 60-61 0.0 1.4275592211046548E-4 0.0 0.18708163592576502 8.56535532662793E-4 62-63 0.0 1.4275592211046548E-4 0.0 0.22545918631979517 9.04120840032948E-4 64-65 0.0 1.4275592211046548E-4 0.0 0.2621236656484997 9.04120840032948E-4 66-67 0.0 1.4275592211046548E-4 0.0 0.3289810225035677 9.04120840032948E-4 68-69 0.0 1.4275592211046548E-4 0.0 0.4142301006572007 9.04120840032948E-4 70-71 0.0 1.4275592211046548E-4 0.0 0.49319791823797315 9.279134937180257E-4 72-73 0.0 1.4275592211046548E-4 0.0 0.5653134515574433 0.0010706694158284912 74-75 0.0 1.4275592211046548E-4 0.0 0.6761396324225346 0.001189632684253879 76-77 0.0 1.4275592211046548E-4 0.0 0.7721905753491929 0.001189632684253879 78-79 0.0 1.4275592211046548E-4 0.0 0.8607468323650516 0.001189632684253879 80-81 0.0 1.4275592211046548E-4 0.0 0.970335795238519 0.0012848032989941894 82-83 0.0 1.4275592211046548E-4 0.0 1.139620526207846 0.0014037665674195773 84-85 0.0 1.4275592211046548E-4 0.0 1.3082390628739908 0.0014275592211046549 86-87 0.0 1.4275592211046548E-4 0.0 1.5032674451305716 0.00147514452847481 88-89 0.0 1.4275592211046548E-4 0.0 1.7483793633942408 0.001522729835844965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5425 0.0 59.99953 1 GTATCAA 9875 0.0 46.723907 1 TATCAAC 13405 0.0 34.023483 2 ATCAACG 13440 0.0 33.793484 3 TGGTATC 1825 0.0 33.32126 2 TCAACGC 13655 0.0 33.29619 4 CAACGCA 14010 0.0 32.419357 5 AACGCAG 14400 0.0 31.665771 6 TAAGGTG 1090 0.0 29.203371 5 CGCAGAG 16965 0.0 26.710754 8 ACGCAGA 17075 0.0 26.622135 7 GTAAGGT 1095 0.0 26.466105 4 GGTAAGG 1110 0.0 25.25244 3 GTGGTAT 2560 0.0 25.24387 1 GTACATG 10885 0.0 24.79573 1 TACATGG 10785 0.0 24.404165 2 GCAGAGT 18555 0.0 24.191483 9 GAGTACT 11945 0.0 22.983862 12-13 GGACCGA 900 0.0 22.693804 6 ACATGGG 10870 0.0 22.639902 3 >>END_MODULE