FastQCFastQC Report
Thu 26 May 2016
SRR937901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010929
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112970.5617801523574427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87060.4329342308952728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57780.2873298858388337No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29380.14610162765567558No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23570.11720950864003651No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.11218695438774814No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21250.10567255233775036No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20560.10224130240301871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50300.055.385271
GTATCAA92050.046.7400741
TGGTATC18250.038.27962
TATCAAC121200.035.6037862
ATCAACG120700.035.5526353
TCAACGC123100.034.8638344
CAACGCA125100.034.2676125
GTGGTAT21500.034.0523341
AACGCAG128700.033.4434436
ACGCAGA150600.028.4231347
CGCAGAG151200.028.1846588
GTACATG106900.027.3947681
TACATGG104500.027.0136382
GCAGAGT162350.025.3704479
ACATGGG106500.025.3002453
GGACCGA7750.025.1329526
CATGGGA57450.023.4949074
TAAGGTG9250.023.1209355
GAGTACT102400.022.73076812-13
GTACGCT1050.002190985522.6293453
Produced by FastQC (version 0.11.5)