##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937901_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2010929 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483851493513693 31.0 31.0 34.0 30.0 34.0 2 31.597236401683002 33.0 31.0 34.0 30.0 34.0 3 31.604416665133378 33.0 31.0 34.0 28.0 34.0 4 35.23709638679436 37.0 35.0 37.0 33.0 37.0 5 35.223273919665985 37.0 35.0 37.0 33.0 37.0 6 35.26992250845256 37.0 35.0 37.0 33.0 37.0 7 35.20216924615439 37.0 35.0 37.0 32.0 37.0 8 35.17738120043025 37.0 35.0 37.0 32.0 37.0 9 36.76166687138134 39.0 37.0 39.0 33.0 39.0 10-11 36.634040784135095 39.0 37.0 39.0 32.0 39.0 12-13 36.483390512544204 39.0 37.0 39.0 32.0 39.0 14-15 37.555564865790885 40.0 37.0 41.0 32.0 41.0 16-17 37.47652055343575 40.0 37.0 41.0 32.0 41.0 18-19 37.42482852452772 40.0 37.0 41.0 32.0 41.0 20-21 37.336599899847286 40.0 37.0 41.0 32.0 41.0 22-23 37.1317567651568 39.5 36.5 41.0 31.0 41.0 24-25 36.941083698131564 39.0 36.0 41.0 31.0 41.0 26-27 36.860233255376 39.0 36.0 41.0 30.5 41.0 28-29 36.886185936947555 39.0 36.0 41.0 30.5 41.0 30-31 36.662754627338906 39.0 36.0 41.0 30.0 41.0 32-33 36.370937014683264 39.0 35.0 40.0 30.0 41.0 34-35 36.352756114213875 39.0 35.0 41.0 30.0 41.0 36-37 36.227783775558464 39.0 35.0 41.0 30.0 41.0 38-39 36.05722454646583 39.0 35.0 41.0 29.0 41.0 40-41 35.779106820777855 39.0 35.0 40.5 28.0 41.0 42-43 35.46785987968745 38.5 35.0 40.0 27.0 41.0 44-45 35.18395651959865 38.0 35.0 40.0 25.5 41.0 46-47 34.770969288323954 38.0 34.0 40.0 24.0 41.0 48-49 34.33857286855975 38.0 34.0 40.0 22.5 41.0 50-51 33.354329019075266 37.5 32.0 39.5 20.5 40.5 52-53 33.41645702061087 37.5 33.0 39.5 20.0 40.5 54-55 33.736487464251596 38.0 33.0 40.0 19.5 41.0 56-57 33.48731556409997 38.0 33.0 40.0 18.5 41.0 58-59 33.2041844838878 37.0 33.0 40.0 16.5 41.0 60-61 32.85166060064776 37.0 32.0 40.0 13.5 41.0 62-63 32.45709744103347 36.0 32.0 39.5 9.5 41.0 64-65 32.05020589986022 36.0 31.0 39.0 8.0 41.0 66-67 31.62514315522826 35.0 31.0 39.0 6.5 40.0 68-69 31.10741105230468 35.0 30.5 38.5 2.0 40.0 70-71 30.638925591107395 35.0 30.0 37.5 2.0 40.0 72-73 30.183725034548708 35.0 29.0 37.0 2.0 39.0 74-75 29.720970258025023 34.0 29.0 36.5 2.0 39.0 76-77 29.228841495647035 34.0 29.0 36.0 2.0 39.0 78-79 28.6963662068626 34.0 27.5 35.5 2.0 37.5 80-81 28.172979254861808 34.0 26.5 35.0 2.0 37.0 82-83 27.636484430827743 33.5 26.0 35.0 2.0 36.5 84-85 27.16398888275021 33.0 25.5 35.0 2.0 36.0 86-87 26.680873367483386 33.0 25.0 35.0 2.0 36.0 88-89 26.1663422726511 32.0 24.0 35.0 2.0 35.0 90-91 25.634725044991644 32.0 21.5 35.0 2.0 35.0 92-93 25.036212864800298 32.0 19.0 34.0 2.0 35.0 94-95 24.362301950988822 31.0 12.0 34.0 2.0 35.0 96-97 23.586578641016168 31.0 2.0 34.0 2.0 35.0 98-99 22.595391234598537 31.0 2.0 34.0 2.0 35.0 100-101 20.275048248844193 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1339034092247111 1101 2 0.1710108635365053 1101 3 0.14552157085770645 1101 4 0.15898772113258275 1101 5 0.11297414853829224 1101 6 0.007858260368195147 1101 7 0.09105640970021511 1101 8 0.11936040039946505 1101 9 0.22240846702411687 1101 10-11 0.18350945307177113 1101 12-13 0.2887908456098671 1101 14-15 0.4336936348781677 1101 16-17 0.5222067419659169 1101 18-19 0.4374869733698077 1101 20-21 0.37947726519480085 1101 22-23 0.42056239454853284 1101 24-25 0.5382372623069998 1101 26-27 0.415761156658391 1101 28-29 0.20859053394674731 1101 30-31 0.32256241932358165 1101 32-33 0.3128785752834773 1101 34-35 0.4692532819681219 1101 36-37 0.5625406714456247 1101 38-39 0.42226836847469684 1101 40-41 0.4497564167424457 1101 42-43 0.4793138688161136 1101 44-45 0.6088195284555127 1101 46-47 0.5955379560246925 1101 48-49 0.545069650659741 1101 50-51 0.38550839806026005 1101 52-53 0.4565470303501513 1101 54-55 0.39378015096642116 1101 56-57 0.537014436971667 1101 58-59 0.6488007746784277 1101 60-61 0.6080344361839352 1101 62-63 0.8436884638023727 1101 64-65 0.5120643868804535 1101 66-67 0.731569221414528 1101 68-69 0.6912488892210114 1101 70-71 0.6831917978494779 1101 72-73 0.7150354992689536 1101 74-75 0.6637505281236571 1101 76-77 0.7206273761059272 1101 78-79 0.8239814172686195 1101 80-81 0.8515106590642922 1101 82-83 0.7994118697696813 1101 84-85 0.9048947847970048 1101 86-87 1.036562329142786 1101 88-89 0.9629159619981777 1101 90-91 1.1968949504963113 1101 92-93 1.2937437200160389 1101 94-95 1.270095741932753 1101 96-97 1.4394299816453895 1101 98-99 1.7486190102679657 1101 100-101 1.975279164699387 1102 1 0.25864382367022287 1102 2 0.23729720904015394 1102 3 0.339552123299395 1102 4 0.29234704358020025 1102 5 0.22264915109112593 1102 6 0.2376580708469973 1102 7 0.09627661102746288 1102 8 0.30579487257127624 1102 9 0.3935875923869929 1102 10-11 0.3619490756767334 1102 12-13 0.4735528562195199 1102 14-15 0.5164150862968526 1102 16-17 0.5686781743441074 1102 18-19 0.4516247130427544 1102 20-21 0.5900873493096341 1102 22-23 0.7248023060426263 1102 24-25 0.7389461896805969 1102 26-27 0.5758124466079622 1102 28-29 0.4098391773695198 1102 30-31 0.6339327884611521 1102 32-33 0.6746661336438038 1102 34-35 0.6886218104294954 1102 36-37 0.7791380306732947 1102 38-39 0.8015755423164848 1102 40-41 0.7195473999489934 1102 42-43 0.6440918113922081 1102 44-45 0.7111715056873962 1102 46-47 0.8227771241398543 1102 48-49 0.8039973909551534 1102 50-51 0.8373913018680028 1102 52-53 0.7577545650870903 1102 54-55 0.7556562829362079 1102 56-57 0.7848475334505096 1102 58-59 0.6167135272284057 1102 60-61 0.820217783179416 1102 62-63 0.8288241760611399 1102 64-65 0.7670700651822244 1102 66-67 0.70160698837417 1102 68-69 0.8807640681237174 1102 70-71 0.7782712423594269 1102 72-73 0.7537747000115402 1102 74-75 0.9822401656695376 1102 76-77 1.0720143053115407 1102 78-79 1.0822569850000683 1102 80-81 1.1933994320490058 1102 82-83 0.9638304404398532 1102 84-85 1.067734639009732 1102 86-87 1.0717678001710347 1102 88-89 1.0920333419644805 1102 90-91 1.039823570133965 1102 92-93 1.198823933668372 1102 94-95 1.3208592332825297 1102 96-97 1.575243319627429 1102 98-99 1.7949478034045825 1102 100-101 2.20432939044926 1103 1 0.2662306028276795 1103 2 0.21659161285319328 1103 3 0.13418801153986237 1103 4 0.123133570520082 1103 5 0.055933223178939784 1103 6 0.1419210295649762 1103 7 0.10223065356947103 1103 8 0.19022580118954124 1103 9 0.21058206527524703 1103 10-11 0.23496371064543098 1103 12-13 0.21858704899738512 1103 14-15 0.24461780617442486 1103 16-17 0.2837481198392737 1103 18-19 0.24413278915029224 1103 20-21 0.2035713016378864 1103 22-23 0.2427925129070161 1103 24-25 0.37564204089849085 1103 26-27 0.2561373409425869 1103 28-29 0.26815425667464865 1103 30-31 0.17363762602492727 1103 32-33 0.12311807163593613 1103 34-35 0.26786974446759615 1103 36-37 0.2417922594228159 1103 38-39 0.25665995967089117 1103 40-41 0.1404565545494023 1103 42-43 0.14703270774982258 1103 44-45 0.061030071178031164 1103 46-47 0.046848193886113165 1103 48-49 0.2755783047403071 1103 50-51 0.19839412946349455 1103 52-53 0.12241285077384845 1103 54-55 0.18939032913450404 1103 56-57 0.193238584543181 1103 58-59 0.06985978824513239 1103 60-61 0.17557828244760998 1103 62-63 0.2969165726426226 1103 64-65 0.39390600443545054 1103 66-67 0.2304130490399423 1103 68-69 0.2658381296696213 1103 70-71 0.4328675355054372 1103 72-73 0.5851504461825705 1103 74-75 0.36282816978996735 1103 76-77 0.4797611287822292 1103 78-79 0.49250627974323535 1103 80-81 0.5224505947786042 1103 82-83 0.5199313362282361 1103 84-85 0.4664266982064227 1103 86-87 0.5219715227886255 1103 88-89 0.5192536831369203 1103 90-91 0.810933929554956 1103 92-93 0.935458263064092 1103 94-95 0.7416414033389209 1103 96-97 1.182273775118798 1103 98-99 1.3506223157456176 1103 100-101 1.6893780550863617 1104 1 0.17267508383894992 1104 2 0.23719948006498726 1104 3 0.14867269355615775 1104 4 0.1905118135403896 1104 5 0.2327862118840116 1104 6 0.07556173519218845 1104 7 0.14374068061486156 1104 8 0.2223796434486971 1104 9 0.3798203642427964 1104 10-11 0.3236728946848544 1104 12-13 0.2652113333072563 1104 14-15 0.35212585439200694 1104 16-17 0.39931440635159987 1104 18-19 0.34465270611546117 1104 20-21 0.4308691046749047 1104 22-23 0.41751319861937475 1104 24-25 0.2921314991047197 1104 26-27 0.4306729071025259 1104 28-29 0.255800295432671 1104 30-31 0.40517682917755593 1104 32-33 0.36221131282086816 1104 34-35 0.4300339449831867 1104 36-37 0.39827893292466143 1104 38-39 0.5171801297910221 1104 40-41 0.5937573785687391 1104 42-43 0.7588422803820905 1104 44-45 0.6786094668971785 1104 46-47 0.5065490913203874 1104 48-49 0.5552438448674764 1104 50-51 0.5077547444612378 1104 52-53 0.5249914448356847 1104 54-55 0.5648175281016279 1104 56-57 0.6046306624811493 1104 58-59 0.6212723928297734 1104 60-61 0.6114050864930292 1104 62-63 0.7015657631796941 1104 64-65 0.6766732200264158 1104 66-67 0.5551085247391789 1104 68-69 0.6637475972425833 1104 70-71 0.7113580147699388 1104 72-73 0.8260616563053951 1104 74-75 1.0003904564541877 1104 76-77 0.9386055186577167 1104 78-79 1.0864399489287955 1104 80-81 1.1084483838920995 1104 82-83 1.2622180215439833 1104 84-85 1.1763153522237815 1104 86-87 1.458270882728545 1104 88-89 1.4216835486694492 1104 90-91 1.4501586003480504 1104 92-93 1.6332371448787057 1104 94-95 1.8002204024486552 1104 96-97 2.001176221861325 1104 98-99 2.0784390864167754 1104 100-101 2.241299606588626 1105 1 0.26782043584552895 1105 2 0.17380594150716533 1105 3 0.29173726974790526 1105 4 0.25187491249528904 1105 5 0.2171618092283012 1105 6 0.27656853022681815 1105 7 0.2881974672247196 1105 8 0.2273817602598598 1105 9 0.3270756601645033 1105 10-11 0.35341957822937076 1105 12-13 0.3130910885217659 1105 14-15 0.34128817062235584 1105 16-17 0.43699152114506745 1105 18-19 0.45506086836939375 1105 20-21 0.4136590405475644 1105 22-23 0.4890077876535841 1105 24-25 0.42092034329751016 1105 26-27 0.4391005057891064 1105 28-29 0.49075359107401795 1105 30-31 0.48198857882463386 1105 32-33 0.5352570854994241 1105 34-35 0.4745498111554127 1105 36-37 0.5401831567770898 1105 38-39 0.658607363168386 1105 40-41 0.6547057878453302 1105 42-43 0.7283217926234897 1105 44-45 0.6137580542974064 1105 46-47 0.5359118816031625 1105 48-49 0.6903105562774385 1105 50-51 0.558562460131732 1105 52-53 0.6219392721661876 1105 54-55 0.6503425397476335 1105 56-57 0.5991650065033198 1105 58-59 0.5846968693968861 1105 60-61 0.3863369095984055 1105 62-63 0.47972607728898 1105 64-65 0.5512764064289826 1105 66-67 0.5041546145854205 1105 68-69 0.43727198871247097 1105 70-71 0.6142873557549429 1105 72-73 0.6263778682470402 1105 74-75 0.7507557656714496 1105 76-77 0.7603664789961826 1105 78-79 0.9763032907152152 1105 80-81 1.0566994306162805 1105 82-83 0.9892684965167646 1105 84-85 1.089882200270722 1105 86-87 1.109452084595869 1105 88-89 1.0514430109569375 1105 90-91 1.2309468753381054 1105 92-93 1.5674154681660326 1105 94-95 1.5553081828553381 1105 96-97 1.6585611317587983 1105 98-99 1.6408151042914412 1105 100-101 1.8720479170334947 1106 1 0.1351744493533893 1106 2 0.1564015510258514 1106 3 0.0470712520049652 1106 4 0.015431868359939926 1106 5 0.04847323284741378 1106 6 -0.09332055504688697 1106 7 0.09342343783530538 1106 8 -0.07012073724263956 1106 9 0.020455357390353868 1106 10-11 -0.3014578093403202 1106 12-13 -0.25802860375177517 1106 14-15 -0.41036082141125263 1106 16-17 -0.4052132825981971 1106 18-19 -0.08883924860764836 1106 20-21 -0.06952935225621815 1106 22-23 -0.13276851296887315 1106 24-25 -0.21584652231550905 1106 26-27 0.015737117041105364 1106 28-29 0.07728778337745013 1106 30-31 0.1345738141021826 1106 32-33 0.05647048748205208 1106 34-35 0.09154478199653937 1106 36-37 0.06086205053291138 1106 38-39 0.03964726343905767 1106 40-41 0.07716431222692677 1106 42-43 0.13865675680460754 1106 44-45 0.14980128457140296 1106 46-47 0.11462355454560935 1106 48-49 0.09963499252495467 1106 50-51 -0.007646758000561249 1106 52-53 -0.06544812675966938 1106 54-55 -0.11507943144357569 1106 56-57 -0.20579520422612063 1106 58-59 -0.2550447991909337 1106 60-61 -0.10146481858793521 1106 62-63 0.0106782447796121 1106 64-65 -0.06903511965177245 1106 66-67 0.13774773087084569 1106 68-69 0.019159759061778914 1106 70-71 0.20162373732209105 1106 72-73 0.11730702456081232 1106 74-75 0.3179212924523611 1106 76-77 0.5872020366009707 1106 78-79 0.6460359085247518 1106 80-81 0.6402272276056173 1106 82-83 0.2576078494715013 1106 84-85 0.3384746334785369 1106 86-87 0.49891475867393 1106 88-89 0.46967349841819583 1106 90-91 0.6296761375289037 1106 92-93 0.7746855485130375 1106 94-95 0.8136588065905777 1106 96-97 1.0675687907664582 1106 98-99 1.2067980529666968 1106 100-101 1.5205696944014235 1107 1 0.10871290294299385 1107 2 0.1252576310072513 1107 3 0.11094141139942337 1107 4 0.1770996975469501 1107 5 0.0781200850006627 1107 6 0.11540609766954191 1107 7 0.15305943979867465 1107 8 0.1877960263007239 1107 9 0.22982914643166197 1107 10-11 0.20127863438077753 1107 12-13 0.12991153936739863 1107 14-15 0.33077496096493775 1107 16-17 0.26591617050056016 1107 18-19 0.3288517276511058 1107 20-21 0.3790943528252839 1107 22-23 0.37391016640801666 1107 24-25 0.47817360220333427 1107 26-27 0.49367118922069864 1107 28-29 0.3627866337740002 1107 30-31 0.2693351099248531 1107 32-33 0.29586830194380553 1107 34-35 0.4145071421711748 1107 36-37 0.2645369065095551 1107 38-39 0.17876865639676254 1107 40-41 0.3397770121158459 1107 42-43 0.436245172935223 1107 44-45 0.3695647762658467 1107 46-47 0.35179514254839717 1107 48-49 0.36954767433021374 1107 50-51 0.2887526581558859 1107 52-53 0.39494222173809135 1107 54-55 0.4374206331408885 1107 56-57 0.5528184256790709 1107 58-59 0.528879481983985 1107 60-61 0.6018244681412526 1107 62-63 0.587064227367641 1107 64-65 0.6645843228551342 1107 66-67 0.6235346279748732 1107 68-69 0.6632179884141287 1107 70-71 0.8861021531943685 1107 72-73 0.9618444706327871 1107 74-75 0.9977418768125865 1107 76-77 1.0098879752715604 1107 78-79 1.2007835124411557 1107 80-81 1.1099713439609857 1107 82-83 1.169128426081592 1107 84-85 1.3480189255036485 1107 86-87 1.4446922264462785 1107 88-89 1.420142381623652 1107 90-91 1.4744841925032013 1107 92-93 1.5446757316745732 1107 94-95 1.5134573030642677 1107 96-97 1.632941184783352 1107 98-99 1.92046089623312 1107 100-101 2.644223754180807 1108 1 0.07842553284523035 1108 2 -0.07416855241613618 1108 3 0.05988251163479674 1108 4 0.03912972395805525 1108 5 -0.036227601810431054 1108 6 0.1225813704853067 1108 7 0.06632832505661668 1108 8 -0.021859969635357857 1108 9 0.10640470468965901 1108 10-11 0.08757894687184375 1108 12-13 0.1467203040710814 1108 14-15 0.13564415526870022 1108 16-17 0.19916454555435337 1108 18-19 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90-91 0.12743511746277392 2316 92-93 -0.07874109459970668 2316 94-95 0.021703377863850903 2316 96-97 0.3573160390338863 2316 98-99 0.5366447259748028 2316 100-101 0.639282608293712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9179.0 3 2066.0 4 2097.0 5 2346.0 6 2767.0 7 3240.0 8 3983.0 9 4866.0 10 6540.0 11 8026.0 12 10602.0 13 13072.0 14 14591.0 15 17884.0 16 21890.0 17 27097.0 18 23656.0 19 17940.0 20 17479.0 21 18329.0 22 19477.0 23 21378.0 24 23811.0 25 26841.0 26 30406.0 27 35193.0 28 41381.0 29 48923.0 30 58799.0 31 71881.0 32 89024.0 33 112690.0 34 149180.0 35 201354.0 36 275167.0 37 333312.0 38 220646.0 39 23813.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34332323118944 18.411094897793742 14.580661253913021 22.664920617103803 2 15.50366099395487 19.708417293458094 40.72676216507392 24.061159547513117 3 19.380474349262176 24.526929945784524 29.18708703152437 26.90550867342893 4 12.604869798146362 15.975812069332617 36.37339606863851 35.04592206388251 5 13.419276216917526 37.73773159851969 33.51907088343261 15.32392130113018 6 32.48248956650277 35.45061644101872 17.075401267269967 14.991492725208547 7 29.014755224926358 33.137238193054294 19.829180451880944 18.0188261301384 8 27.08941071513122 34.277504903023555 19.105269846965857 19.527814534879372 9 27.043891936074722 16.510475203161874 20.01275913885781 36.432873721905594 10-11 25.22089358232373 26.754588824937848 26.164550766648258 21.85996682609016 12-13 26.281499386365475 24.03767778269239 27.279312729334222 22.40151010160791 14-15 23.20419424810901 25.06960303903576 26.121603791568393 25.60459892128683 16-17 21.99338069807172 28.55011273046952 26.849312952142874 22.607193619315883 18-19 21.97163872718148 26.446956675150595 29.91987696227185 21.661527635396073 20-21 22.64451900746635 25.34027372385986 30.434494458475882 21.58071281019791 22-23 22.376602685872946 25.39155174497037 30.34620381592764 21.88564175322904 24-25 22.02492099300304 25.622831745304115 30.418833623783442 21.933413637909407 26-27 21.38526470953922 26.05746400993273 30.269529643078098 22.28774163744995 28-29 21.696710579108935 26.42055861889878 30.1573610866747 21.725369715317587 30-31 21.776701368650496 25.859976162062093 30.169569324765366 22.193753144522045 32-33 21.50679547969829 25.864689497777206 30.173589573810023 22.454925448714484 34-35 22.114285557653986 26.07747767099014 30.056592823468815 21.751643947887057 36-37 22.211688977905183 25.65303692534681 29.869197037082806 22.266077059665196 38-39 21.822347548472706 26.077421791652693 29.80019443396645 22.30003622590815 40-41 22.148185720948216 25.960752737462677 29.821751019448246 22.06931052214086 42-43 21.730921029026586 25.93937246600409 29.707775704734914 22.621930800234413 44-45 21.880418287091885 26.244896423591047 29.752315062237034 22.12237022708003 46-47 22.32206169395005 26.587757384103966 28.8330741283162 22.257106793629784 48-49 22.20289927300138 26.70378699625599 28.6225151535917 22.470798577150937 50-51 22.556919564979015 27.088155546916276 28.275092955331598 22.079831932773107 52-53 22.55224222847002 26.80639468804211 28.341957288987256 22.299405794500608 54-55 22.221665732261883 27.02288668784651 28.428057060985484 22.327390518906125 56-57 22.378320722343673 27.32530275342709 28.082833975341316 22.213542548887926 58-59 22.24111496521961 26.752041051445087 28.361691130222503 22.645152853112798 60-61 22.060322250446976 27.063028887150065 28.604902537412748 22.271746324990215 62-63 22.034563376155127 27.058674926080833 28.859791262440694 22.046970435323345 64-65 21.96167522964347 26.959240230421923 28.75149929939281 22.327585240541804 66-67 21.864952489428394 27.162763629986042 28.412198739727966 22.5600851408576 68-69 22.23285183671019 26.844925301629846 28.704393659800353 22.21782920185962 70-71 22.21345616333933 26.8965577372441 28.37522607131647 22.514760028100103 72-73 22.04779922095082 27.22043464526756 28.138293684998274 22.59347244878335 74-75 22.004177913858243 27.605173438233766 27.971070998376916 22.419577649531075 76-77 22.23050566869298 27.256520037205746 28.044513336584142 22.468460957517134 78-79 22.273719578455932 27.284370809869852 28.031913077778537 22.409996533895686 80-81 22.34790999812884 27.551792535357006 27.65205262857597 22.44824483793818 82-83 22.369796954630903 27.48887230652513 27.73024951197605 22.411081226867914 84-85 22.338352978927055 27.60462107015463 27.654721195666525 22.40230475525179 86-87 22.36083152293066 27.671083052637872 27.499775775815678 22.46830964861579 88-89 22.34501285252891 27.62562453174912 27.41020732722461 22.619155288497353 90-91 22.342801773678804 27.526031006241563 27.491249082178275 22.63991813790136 92-93 22.25735702823322 27.187114683611103 27.7877255342701 22.767802753885583 94-95 22.19974667044037 27.21720667785489 27.749029335204238 22.834017316500503 96-97 22.332832278993433 27.039770295565596 27.66173700277166 22.965660422669313 98-99 22.016861825064233 27.223469133283455 27.65424514047244 23.10542390117987 100-101 22.409344785383663 26.574999445731923 28.09122779222196 22.924427976662447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3128.0 1 1870.5 2 650.0 3 679.5 4 571.5 5 579.5 6 972.5 7 1880.0 8 3055.0 9 3686.0 10 3407.5 11 3011.0 12 3131.5 13 3288.5 14 3429.0 15 3611.5 16 3750.0 17 3909.0 18 4040.5 19 4256.5 20 4654.0 21 5379.0 22 6332.0 23 7608.5 24 9145.5 25 11024.5 26 13553.5 27 16438.5 28 18662.0 29 20663.5 30 23553.5 31 27370.0 32 33386.0 33 36182.0 34 36858.0 35 39837.5 36 44227.0 37 48826.5 38 51474.5 39 53704.5 40 55527.0 41 58538.0 42 60498.5 43 62402.0 44 66033.0 45 69181.0 46 71269.0 47 72621.5 48 71918.5 49 70894.5 50 70793.0 51 68308.0 52 66543.5 53 67434.0 54 68679.5 55 65011.5 56 59286.5 57 54966.5 58 51484.5 59 46349.0 60 37489.5 61 31175.5 62 26444.0 63 19593.5 64 14329.0 65 11573.0 66 8565.0 67 6172.0 68 4785.5 69 3520.5 70 2559.5 71 1777.5 72 1278.5 73 1037.5 74 784.5 75 572.0 76 411.5 77 274.5 78 181.5 79 127.5 80 93.5 81 61.5 82 38.5 83 25.5 84 19.0 85 10.5 86 10.0 87 10.0 88 7.0 89 6.0 90 5.0 91 5.0 92 4.0 93 4.5 94 5.5 95 4.5 96 2.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31845977655103685 2 0.2988171138811962 3 0.2875287988785283 4 0.27181466874265575 5 0.2690796144468552 6 0.21935135452320792 7 0.21134510467550072 8 0.22447336529534356 9 0.22497064789458007 10-11 0.2254182022338929 12-13 0.22086806645087914 14-15 0.21738708825622385 16-17 0.21746168064610935 18-19 0.21830706106481132 20-21 0.20515393631500667 22-23 0.19625257778867378 24-25 0.1982417081856197 26-27 0.21032567534706595 28-29 0.22815325652969348 30-31 0.2219620881691994 32-33 0.22668627286194593 34-35 0.21892866431385694 36-37 0.20167295812035133 38-39 0.2027918439686334 40-41 0.19697363755756667 42-43 0.20970407209802036 44-45 0.20515393631500667 46-47 0.2064717352029833 48-49 0.21838165345469682 50-51 0.22820298478961715 52-53 0.2431214627667113 54-55 0.21403043071137767 56-57 0.18324863781864006 58-59 0.18357187150814375 60-61 0.19023545833791247 62-63 0.19926113751405444 64-65 0.1860582845043261 66-67 0.1940396702220715 68-69 0.19679958864783392 70-71 0.18991222464840876 72-73 0.19229918112474384 74-75 0.19625257778867378 76-77 0.2113202405455389 78-79 0.2164919795775982 80-81 0.20629768629325052 82-83 0.20445774067607558 84-85 0.19632717017855927 86-87 0.19936059403390174 88-89 0.20515393631500667 90-91 0.2058501319539377 92-93 0.20167295812035133 94-95 0.20174755051023682 96-97 0.19035977898772158 98-99 0.19739632776691768 100-101 0.18784850186157742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2010929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.12759602128993 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56442769382518 37.87863105016042 2 13.074453881607425 13.107818847524063 3 4.573352881779287 6.8775355716190285 4 2.1573998601182516 4.325810745775806 5 1.1900749462172726 2.982779806951889 6 0.6904537206732764 2.0766471108783935 7 0.49064486932322676 1.7216393459547312 8 0.351587595372575 1.409939275754655 9 0.2637655421161739 1.1899739285582493 >10 1.451476661260971 13.572948064024082 >50 0.1188877290640093 4.152754281125367 >100 0.06603944219346168 6.058393591282231 >500 0.005254191357176684 1.8509152454804925 >1k 0.0018835780337048478 1.513043453770728 >5k 1.9827137196893138E-4 0.7197727652778673 >10k+ 9.913568598446569E-5 0.5613969158619212 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11297 0.5617801523574427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8706 0.4329342308952728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5778 0.2873298858388337 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2938 0.14610162765567558 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2357 0.11720950864003651 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 0.11218695438774814 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2125 0.10567255233775036 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2056 0.10224130240301871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.972825992364723E-5 2 0.0 0.0 0.0 0.0 4.972825992364723E-5 3 0.0 0.0 0.0 0.0 4.972825992364723E-5 4 0.0 0.0 0.0 0.0 4.972825992364723E-5 5 0.0 0.0 0.0 0.0 4.972825992364723E-5 6 0.0 0.0 0.0 0.0 4.972825992364723E-5 7 0.0 0.0 0.0 0.0 4.972825992364723E-5 8 0.0 0.0 0.0 0.0 4.972825992364723E-5 9 0.0 0.0 0.0 0.0 4.972825992364723E-5 10-11 0.0 0.0 0.0 0.0 4.972825992364723E-5 12-13 0.0 0.0 0.0 0.0 2.9836955954188336E-4 14-15 0.0 0.0 0.0 0.0 2.9836955954188336E-4 16-17 0.0 0.0 0.0 0.0 2.9836955954188336E-4 18-19 0.0 0.0 0.0 0.0 3.480978194655306E-4 20-21 0.0 0.0 0.0 0.0 3.480978194655306E-4 22-23 0.0 0.0 0.0 4.972825992364723E-5 3.9782607938917784E-4 24-25 0.0 0.0 0.0 1.4918477977094168E-4 3.9782607938917784E-4 26-27 0.0 0.0 0.0 1.9891303969458892E-4 4.972825992364723E-4 28-29 0.0 0.0 0.0 9.199728085874737E-4 4.972825992364723E-4 30-31 0.0 0.0 0.0 0.0030831521152661287 4.972825992364723E-4 32-33 0.0 0.0 0.0 0.006240896620417727 5.470108591601196E-4 34-35 0.0 0.0 0.0 0.008478668316981852 6.46467379007414E-4 36-37 0.0 0.0 0.0 0.010119700894462211 6.46467379007414E-4 38-39 0.0 0.0 0.0 0.01113913022289698 6.46467379007414E-4 40-41 0.0 0.0 0.0 0.013451494309346576 6.46467379007414E-4 42-43 0.0 0.0 0.0 0.01583845078568164 6.46467379007414E-4 44-45 0.0 0.0 0.0 0.01869782573129136 6.713315089692376E-4 46-47 0.0 0.0 0.0 0.02270095065514496 6.961956389310612E-4 48-49 0.0 0.0 0.0 0.027673776647509682 7.459238988547084E-4 50-51 0.0 0.0 0.0 0.035953531924796944 7.956521587783557E-4 52-53 0.0 2.4864129961823615E-5 0.0 0.05174225445055494 7.956521587783557E-4 54-55 0.0 4.972825992364723E-5 0.0 0.0751891290045546 8.951086786256502E-4 56-57 0.0 4.972825992364723E-5 0.0 0.09980461766675999 8.951086786256502E-4 58-59 0.0 4.972825992364723E-5 0.0 0.13590733437132788 9.199728085874737E-4 60-61 0.0 4.972825992364723E-5 0.0 0.18618260515413523 9.448369385492973E-4 62-63 0.0 4.972825992364723E-5 0.0 0.2228074685879014 9.448369385492973E-4 64-65 0.0 4.972825992364723E-5 0.0 0.26047662548006423 9.697010685111209E-4 66-67 0.0 4.972825992364723E-5 0.0 0.32708762964778965 9.945651984729447E-4 68-69 0.0 4.972825992364723E-5 0.0 0.4150569214527216 9.945651984729447E-4 70-71 0.0 4.972825992364723E-5 0.0 0.49228490911414574 0.0010194293284347682 72-73 0.0 4.972825992364723E-5 0.0 0.5635703697146941 0.0011934782381675334 74-75 0.0 4.972825992364723E-5 0.0 0.6785421066581665 0.0013923912778621224 76-77 0.0 4.972825992364723E-5 0.0 0.7732247135527908 0.0014421195377857696 78-79 0.0 4.972825992364723E-5 0.0 0.8564200924050526 0.0014421195377857696 80-81 0.0 4.972825992364723E-5 0.0 0.9601532426057806 0.0014421195377857696 82-83 0.0 4.972825992364723E-5 0.0 1.1354702229665992 0.0015415760576330641 84-85 0.0 4.972825992364723E-5 0.0 1.3080024207716932 0.0015415760576330641 86-87 0.0 4.972825992364723E-5 0.0 1.4950055422145685 0.001616168447518535 88-89 0.0 4.972825992364723E-5 0.0 1.7351184452558992 0.0016907608374040059 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5030 0.0 55.38527 1 GTATCAA 9205 0.0 46.740074 1 TGGTATC 1825 0.0 38.2796 2 TATCAAC 12120 0.0 35.603786 2 ATCAACG 12070 0.0 35.552635 3 TCAACGC 12310 0.0 34.863834 4 CAACGCA 12510 0.0 34.267612 5 GTGGTAT 2150 0.0 34.052334 1 AACGCAG 12870 0.0 33.443443 6 ACGCAGA 15060 0.0 28.423134 7 CGCAGAG 15120 0.0 28.184658 8 GTACATG 10690 0.0 27.394768 1 TACATGG 10450 0.0 27.013638 2 GCAGAGT 16235 0.0 25.370447 9 ACATGGG 10650 0.0 25.300245 3 GGACCGA 775 0.0 25.132952 6 CATGGGA 5745 0.0 23.494907 4 TAAGGTG 925 0.0 23.120935 5 GAGTACT 10240 0.0 22.730768 12-13 GTACGCT 105 0.0021909855 22.629345 3 >>END_MODULE