FastQCFastQC Report
Thu 26 May 2016
SRR937901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010929
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130520.6490532485234436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108120.5376619462944738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72810.36207146050407546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27840.1384434756274339No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24330.12098885639423372No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24180.12024293249537901No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21690.10786059577439083No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21400.10641847623660507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.10288776978202611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.059.16081
GTATCAA97950.046.2803961
TGGTATC18500.037.7449042
TATCAAC132850.034.1113972
ATCAACG132550.034.0452543
TCAACGC134700.033.5723764
CAACGCA137650.032.9218985
AACGCAG141950.032.021826
GTGGTAT23550.030.0641211
TAAGGTG9500.028.5012515
CGCAGAG166550.027.0381168
ACGCAGA167950.026.982437
GTAAGGT9650.026.089234
GTACATG105750.025.2527731
TACATGG104450.025.194982
GCAGAGT179200.024.7848539
ACATGGG103650.023.4646113
GAGTACT116900.022.83676112-13
AGTACTT117600.022.35748712-13
GGACCGA8650.021.96416
Produced by FastQC (version 0.11.5)