##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937901_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2010929 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13656374740232 33.0 31.0 34.0 31.0 34.0 2 32.3412780858996 34.0 31.0 34.0 31.0 34.0 3 32.39423719087049 34.0 31.0 34.0 30.0 34.0 4 36.03188078743705 37.0 35.0 37.0 35.0 37.0 5 35.943365976620754 37.0 35.0 37.0 35.0 37.0 6 35.99130501375235 37.0 35.0 37.0 35.0 37.0 7 35.93969901473399 37.0 35.0 37.0 35.0 37.0 8 35.89997110788099 37.0 35.0 37.0 35.0 37.0 9 37.569739160358225 39.0 37.0 39.0 35.0 39.0 10-11 37.48383508318792 39.0 37.0 39.0 35.0 39.0 12-13 37.36877060303969 39.0 37.0 39.0 34.0 39.0 14-15 38.54319023695018 40.0 38.0 41.0 34.0 41.0 16-17 38.50498351756825 40.0 38.0 41.0 34.0 41.0 18-19 38.50011860189991 40.0 38.0 41.0 34.0 41.0 20-21 38.398008084820496 40.0 38.0 41.0 34.0 41.0 22-23 38.34203271224394 40.0 38.0 41.0 34.0 41.0 24-25 38.34347731819473 40.0 38.0 41.0 34.0 41.0 26-27 38.13699514005716 40.0 38.0 41.0 33.0 41.0 28-29 38.088901696678505 40.0 37.5 41.0 33.0 41.0 30-31 38.05681379103887 40.0 37.0 41.0 33.0 41.0 32-33 37.8899998458424 40.0 37.0 41.0 33.0 41.0 34-35 37.716030749966805 40.0 37.0 41.0 33.0 41.0 36-37 37.50490544420017 40.0 36.5 41.0 33.0 41.0 38-39 37.33015486871988 40.0 36.0 41.0 32.0 41.0 40-41 37.12516354381482 40.0 36.0 41.0 31.5 41.0 42-43 36.85291897426513 39.0 36.0 41.0 31.0 41.0 44-45 36.49683653674496 39.0 35.0 41.0 30.5 41.0 46-47 36.08333586118654 39.0 35.0 41.0 29.5 41.0 48-49 35.662873975162725 39.0 35.0 40.5 28.0 41.0 50-51 35.37220334482222 39.0 35.0 40.0 27.0 41.0 52-53 35.073616721425765 38.0 35.0 40.0 26.0 41.0 54-55 34.81740504015805 38.0 34.0 40.0 26.0 41.0 56-57 34.50870443461703 38.0 34.0 40.0 25.0 41.0 58-59 34.22408722535704 38.0 34.0 40.0 24.5 41.0 60-61 33.85968549859294 37.0 33.0 40.0 23.5 41.0 62-63 33.42597525820156 36.5 33.0 39.5 22.5 41.0 64-65 33.012737147855546 36.0 32.5 39.0 21.5 40.5 66-67 32.5765909686518 35.5 32.0 39.0 20.0 40.0 68-69 32.09052507572371 35.0 31.0 38.0 18.5 40.0 70-71 31.647488051542346 35.0 31.0 37.5 17.5 40.0 72-73 31.17646247082816 35.0 31.0 37.0 14.0 39.0 74-75 30.702778665979753 34.0 30.0 36.5 9.5 39.0 76-77 29.473110686652788 33.5 28.5 35.0 8.0 37.5 78-79 30.07866389116672 34.0 30.0 35.0 7.0 37.5 80-81 29.9307665760452 34.0 30.0 35.0 6.5 37.0 82-83 29.54327328314426 34.0 29.5 35.0 2.0 37.0 84-85 29.13556644714955 34.0 29.0 35.0 2.0 36.0 86-87 28.80087163693994 34.0 29.0 35.0 2.0 36.0 88-89 28.376038636868827 34.0 29.0 35.0 2.0 35.5 90-91 27.853312822083723 33.0 27.0 35.0 2.0 35.0 92-93 27.36963811253406 33.0 26.5 35.0 2.0 35.0 94-95 26.84767338876708 33.0 25.5 35.0 2.0 35.0 96-97 26.294225952283746 32.5 25.0 34.0 2.0 35.0 98-99 25.430675324688238 32.0 22.0 34.0 2.0 35.0 100-101 22.994556247386157 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18908668244695548 1101 2 0.14204821058083184 1101 3 0.12709888532369007 1101 4 0.12126116223122096 1101 5 0.08082779037297172 1101 6 0.010103607228217015 1101 7 0.07138957536481172 1101 8 -0.027031377546627766 1101 9 0.2173371307176808 1101 10-11 0.13712445685440144 1101 12-13 0.1707369690771614 1101 14-15 0.24630482189163416 1101 16-17 0.2611395891422248 1101 18-19 0.24278520425276184 1101 20-21 0.242836561359546 1101 22-23 0.2462307820361147 1101 24-25 0.24922612521496745 1101 26-27 0.09854697536041357 1101 28-29 0.21276441313336392 1101 30-31 0.3228378966335015 1101 32-33 0.2826244191887852 1101 34-35 0.3388205986345909 1101 36-37 0.23516590758175937 1101 38-39 0.3587469079760197 1101 40-41 0.2944364544807385 1101 42-43 0.30864813420703996 1101 44-45 0.31980836156763814 1101 46-47 0.5081740805649346 1101 48-49 0.5053620888428441 1101 50-51 0.5027040701454553 1101 52-53 0.46232284677537194 1101 54-55 0.5171556288456429 1101 56-57 0.5194795826132506 1101 58-59 0.5267754183522371 1101 60-61 0.6057379571732113 1101 62-63 0.5476166328333107 1101 64-65 0.6157429115185167 1101 66-67 0.5987523048753474 1101 68-69 0.6841653920726003 1101 70-71 0.6126185823108727 1101 72-73 0.5538755822960155 1101 74-75 0.703341911284312 1101 76-77 0.5771159266626107 1101 78-79 0.7619363911678825 1101 80-81 0.9124123814312739 1101 82-83 0.7011558373423448 1101 84-85 0.6992107663276208 1101 86-87 0.7162172749851337 1101 88-89 0.9241198109881275 1101 90-91 0.9071476383777899 1101 92-93 1.053818186553869 1101 94-95 1.1455828662243164 1101 96-97 0.864185289976291 1101 98-99 1.0712195996866711 1101 100-101 1.4715412332121502 1102 1 0.17913559726979855 1102 2 0.1780075915885817 1102 3 0.20321201206424888 1102 4 0.11667978160748049 1102 5 0.03351349829707573 1102 6 0.12043962090052673 1102 7 0.14400620715679935 1102 8 0.16187789016776577 1102 9 0.24030515150746368 1102 10-11 0.24671144996089112 1102 12-13 0.2529889427548184 1102 14-15 0.26335693420297446 1102 16-17 0.26616703473762726 1102 18-19 0.30114825487830643 1102 20-21 0.3322770074546071 1102 22-23 0.3189451080746366 1102 24-25 0.30221533409090284 1102 26-27 0.21994773220404085 1102 28-29 0.3715344886428369 1102 30-31 0.2520530084572101 1102 32-33 0.27674395895955683 1102 34-35 0.27697750781805297 1102 36-37 0.40859942876260646 1102 38-39 0.45005830702345406 1102 40-41 0.4895917601245685 1102 42-43 0.37771533374457533 1102 44-45 0.5790034211536081 1102 46-47 0.5824989042498601 1102 48-49 0.515308566687871 1102 50-51 0.43581642078562055 1102 52-53 0.5517145867879449 1102 54-55 0.5395883528640084 1102 56-57 0.48454274725617097 1102 58-59 0.6372840659385304 1102 60-61 0.5785778283108343 1102 62-63 0.6373913455755513 1102 64-65 0.7070083312718651 1102 66-67 0.7126117992986849 1102 68-69 0.5731901815880462 1102 70-71 0.4303822359515692 1102 72-73 0.49206784570151996 1102 74-75 0.5633568691291018 1102 76-77 0.3566066603774338 1102 78-79 0.40763565348699515 1102 80-81 0.4611596478259017 1102 82-83 0.4667871518555735 1102 84-85 0.4411791051173495 1102 86-87 0.5178939113543635 1102 88-89 0.7593546561093696 1102 90-91 0.7369934093452422 1102 92-93 0.8642357782129864 1102 94-95 1.022621070034262 1102 96-97 1.1350694332504006 1102 98-99 1.1662939667285634 1102 100-101 1.5768718976778295 1103 1 0.15842835904131647 1103 2 0.11993569863217601 1103 3 0.18147849788741155 1103 4 0.00482391344370825 1103 5 -0.032069639906993075 1103 6 0.04025873360696863 1103 7 0.07531687374902418 1103 8 0.026020848491654647 1103 9 0.01467934211783728 1103 10-11 0.0452967217242346 1103 12-13 0.16009099125634663 1103 14-15 0.26165660021172954 1103 16-17 0.1262806968514596 1103 18-19 0.12406858192919401 1103 20-21 0.19823223226650555 1103 22-23 0.21084404151392988 1103 24-25 0.0749649982934173 1103 26-27 0.05461905570044934 1103 28-29 0.02212940028457666 1103 30-31 0.153135880103207 1103 32-33 0.09961420374416008 1103 34-35 0.14238346974239136 1103 36-37 0.009640811492928947 1103 38-39 0.03090750521457153 1103 40-41 0.12807552088131047 1103 42-43 0.198821204918751 1103 44-45 0.19380264873727526 1103 46-47 0.19406741514809056 1103 48-49 0.13556922435834196 1103 50-51 0.13200726760832282 1103 52-53 -0.05566699191455626 1103 54-55 0.01227080283373283 1103 56-57 -0.038993728506746095 1103 58-59 0.04621671705834984 1103 60-61 0.2125883943006457 1103 62-63 -0.039477598712167605 1103 64-65 0.18118695473751956 1103 66-67 0.2672728983499155 1103 68-69 0.25772253363736297 1103 70-71 0.27509272761164283 1103 72-73 0.22927825799624912 1103 74-75 0.22322001044209827 1103 76-77 0.08833840114943214 1103 78-79 0.29610791995311203 1103 80-81 0.32887377277650387 1103 82-83 0.34483957019958567 1103 84-85 0.3869895456551937 1103 86-87 0.4680554882396706 1103 88-89 0.6050958024951818 1103 90-91 0.5584602559796572 1103 92-93 0.6861342080928843 1103 94-95 0.6691089621674564 1103 96-97 0.822702780705292 1103 98-99 0.7906194981752996 1103 100-101 1.300236615898033 1104 1 0.15060692609995385 1104 2 0.15313802428225642 1104 3 0.13231264342618232 1104 4 0.08933969132556285 1104 5 0.07664036688599651 1104 6 0.11726424816549041 1104 7 0.12762923319529307 1104 8 0.20000474783725508 1104 9 0.10199353693744229 1104 10-11 0.13715399296710018 1104 12-13 0.08446569854594799 1104 14-15 0.15393706213512104 1104 16-17 0.14439973452513755 1104 18-19 0.13300336921574996 1104 20-21 0.20199527502015968 1104 22-23 0.24360070898225672 1104 24-25 0.1790222281876339 1104 26-27 0.06963483569192164 1104 28-29 0.16113519753278638 1104 30-31 0.18824636113978954 1104 32-33 0.1482811320042643 1104 34-35 0.2818554940115163 1104 36-37 0.17384132708516375 1104 38-39 0.20888175935682796 1104 40-41 0.2857667899892391 1104 42-43 0.39660377857629214 1104 44-45 0.3037320185080503 1104 46-47 0.23157653927417954 1104 48-49 0.3905468834345811 1104 50-51 0.23944833524710418 1104 52-53 0.27850122532614563 1104 54-55 0.3299670717517884 1104 56-57 0.3019964870546872 1104 58-59 0.2949667645249008 1104 60-61 0.29849968880845523 1104 62-63 0.2724066546284192 1104 64-65 0.39622309260570177 1104 66-67 0.38896237005774736 1104 68-69 0.23425981703167054 1104 70-71 0.40215330337036903 1104 72-73 0.1945543656546107 1104 74-75 0.4058180341458417 1104 76-77 0.4348073116127473 1104 78-79 0.48239040289679735 1104 80-81 0.40303656828216106 1104 82-83 0.30539539297424767 1104 84-85 0.43678512303833017 1104 86-87 0.49983698176945524 1104 88-89 0.5507360524434937 1104 90-91 0.6049874975612006 1104 92-93 0.6233802620402003 1104 94-95 0.6778275129397926 1104 96-97 0.8489449276279828 1104 98-99 1.0400013781882969 1104 100-101 1.2045952056562257 1105 1 0.03436815263273019 1105 2 0.07016150460299286 1105 3 0.15700181200789132 1105 4 0.08828553352613966 1105 5 0.10039077573497934 1105 6 0.03869303198313645 1105 7 0.020037173148651277 1105 8 0.06394865729720323 1105 9 0.1170954624568381 1105 10-11 0.025172558604531048 1105 12-13 0.036992314437462426 1105 14-15 0.02882679680452327 1105 16-17 0.15753653008365376 1105 18-19 0.19962646072287527 1105 20-21 0.11153327921413592 1105 22-23 0.2143262371584953 1105 24-25 0.1961386641289664 1105 26-27 0.025475429191963883 1105 28-29 0.11594924381378036 1105 30-31 0.15676357385878248 1105 32-33 0.07168619620948391 1105 34-35 0.22677716491887878 1105 36-37 0.24747024938049833 1105 38-39 0.26181478175254824 1105 40-41 0.3460898904166996 1105 42-43 0.35309870743303406 1105 44-45 0.15476479580676283 1105 46-47 0.1079763603009809 1105 48-49 0.16190154346337948 1105 50-51 0.19469335436919977 1105 52-53 0.13876106920600506 1105 54-55 0.2324357054245283 1105 56-57 0.19927380064432754 1105 58-59 0.2869012892193794 1105 60-61 0.23136137679190227 1105 62-63 0.11750510404655046 1105 64-65 0.11108969102466659 1105 66-67 0.06189734643299971 1105 68-69 0.11778617664036517 1105 70-71 0.21523569192628145 1105 72-73 0.057027657727822145 1105 74-75 0.2004174400227896 1105 76-77 0.2140157658308688 1105 78-79 0.14258008758396912 1105 80-81 0.18611439755248682 1105 82-83 0.16979611594667432 1105 84-85 0.27508552898465766 1105 86-87 0.33017810695016436 1105 88-89 0.4472328361648614 1105 90-91 0.35976253504787437 1105 92-93 0.3192493652343913 1105 94-95 0.5934455085249297 1105 96-97 0.768486103498887 1105 98-99 1.0217844128317886 1105 100-101 1.2400271908852751 1106 1 -0.28177467889471686 1106 2 -0.25594273303970994 1106 3 -0.31986491101267234 1106 4 -0.26547224715471884 1106 5 -0.22003239084203585 1106 6 -0.17265758321362767 1106 7 -0.11951558178871835 1106 8 -0.22182155539608317 1106 9 -0.45800849341635086 1106 10-11 -0.42682245492894566 1106 12-13 -0.5317048004135003 1106 14-15 -0.35289127145200894 1106 16-17 -0.3812817029653459 1106 18-19 -0.4383204214547689 1106 20-21 -0.37225856996232665 1106 22-23 -0.6017497808405992 1106 24-25 -0.490308008856168 1106 26-27 -0.4809691945603518 1106 28-29 -0.5232602646264937 1106 30-31 -0.5733977178409759 1106 32-33 -0.6102412780256756 1106 34-35 -0.878306426703169 1106 36-37 -0.7163317605753576 1106 38-39 -0.6186726795041437 1106 40-41 -0.7173650307305266 1106 42-43 -0.9440680147336948 1106 44-45 -0.9247627279515243 1106 46-47 -0.61189714226483 1106 48-49 -0.6734739784506019 1106 50-51 -0.8390235697323405 1106 52-53 -0.9397973381985523 1106 54-55 -1.1158262889913146 1106 56-57 -1.2309485411164687 1106 58-59 -1.0469714619610642 1106 60-61 -1.055574977452146 1106 62-63 -1.1239387085511083 1106 64-65 -1.305137244433034 1106 66-67 -1.2828931195113142 1106 68-69 -1.1476103926023598 1106 70-71 -1.227272867889969 1106 72-73 -1.2986591768051632 1106 74-75 -1.2201907978931388 1106 76-77 -1.3039039213195878 1106 78-79 -0.9930093230054418 1106 80-81 -1.039434626073458 1106 82-83 -1.4014177129594607 1106 84-85 -1.196643383128869 1106 86-87 -1.1207888747860508 1106 88-89 -1.1429635059545582 1106 90-91 -1.3787480996166046 1106 92-93 -1.2278151087531555 1106 94-95 -1.108130052666013 1106 96-97 -1.3528722390133012 1106 98-99 -1.4394831657434857 1106 100-101 -0.8147921350878988 1107 1 0.06041705946070408 1107 2 0.06293530565930183 1107 3 0.0478247735568047 1107 4 0.068787227386089 1107 5 0.039713404709388556 1107 6 0.048214823720158506 1107 7 0.04397352927072262 1107 8 0.058910315744988395 1107 9 0.0023017703465981754 1107 10-11 0.015829124807034134 1107 12-13 0.03969849081076404 1107 14-15 0.027387862599503876 1107 16-17 0.11706945567992477 1107 18-19 0.20121366622509385 1107 20-21 0.08040747669348747 1107 22-23 0.07770783883442078 1107 24-25 -0.0051424142915976745 1107 26-27 -0.03239974042823235 1107 28-29 0.09281351666468396 1107 30-31 0.10494194476979146 1107 32-33 0.08691183882053366 1107 34-35 0.08404793533150468 1107 36-37 0.06285266681878454 1107 38-39 0.08034692833899015 1107 40-41 0.09252543401927227 1107 42-43 0.07975525634022063 1107 44-45 0.21785720539730846 1107 46-47 0.005478015967106842 1107 48-49 0.06893670123628937 1107 50-51 -0.09366008837141493 1107 52-53 -0.22359632551417263 1107 54-55 -0.07914102199914197 1107 56-57 -0.11282484895185974 1107 58-59 -0.005399088611191871 1107 60-61 0.09475504915665312 1107 62-63 0.0064825113202360285 1107 64-65 -0.006808690350005975 1107 66-67 0.06946098418550406 1107 68-69 0.10199951723772926 1107 70-71 0.09008679007071407 1107 72-73 0.18815461326949645 1107 74-75 0.21245083583544755 1107 76-77 0.058625464611328226 1107 78-79 0.19420876581111202 1107 80-81 0.3439613541895312 1107 82-83 0.4337808196815267 1107 84-85 0.37101723843200674 1107 86-87 0.623483774985317 1107 88-89 0.7844932870393215 1107 90-91 0.7470185179658664 1107 92-93 0.8850423662526552 1107 94-95 0.8946335165297796 1107 96-97 0.7687822857742148 1107 98-99 1.0776426964591046 1107 100-101 1.5465495425581288 1108 1 0.06842725445424946 1108 2 0.029789481626295355 1108 3 -0.0034705286681671055 1108 4 0.03949531322987099 1108 5 0.027863593941312104 1108 6 0.060049973870540896 1108 7 0.0794995045866429 1108 8 0.05894440168113135 1108 9 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82-83 0.08178199714607715 2316 84-85 0.2687833052798858 2316 86-87 0.3951168189223324 2316 88-89 0.5405430846148143 2316 90-91 0.4271992664204447 2316 92-93 0.441752767316963 2316 94-95 0.4970891800195858 2316 96-97 0.8272416804503919 2316 98-99 0.9119470557540019 2316 100-101 1.1919807507208624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 27.0 5 61.0 6 132.0 7 318.0 8 768.0 9 1391.0 10 2375.0 11 3872.0 12 5742.0 13 7963.0 14 10213.0 15 14114.0 16 19084.0 17 25664.0 18 23211.0 19 15024.0 20 12532.0 21 12563.0 22 12977.0 23 13934.0 24 15966.0 25 18756.0 26 22364.0 27 27628.0 28 34023.0 29 41541.0 30 51653.0 31 64483.0 32 81573.0 33 105200.0 34 143345.0 35 202656.0 36 302095.0 37 415934.0 38 281346.0 39 20386.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42851485778987 19.26677160214992 15.227617798702967 22.07709574135725 2 16.070930400824693 20.498237381827007 39.696727234029645 23.734104983318655 3 19.6732455496937 24.580131869399665 29.70542470669029 26.041197874216344 4 13.190725873825418 16.705471304790624 35.872194330179894 34.231608491204064 5 13.330528413982224 38.05270960679905 33.52793704646966 15.088824932749075 6 32.50363389259392 36.114552030429714 16.5026711534818 14.879142923494564 7 28.920712765095136 33.46189746132261 19.563793649601752 18.05359612398051 8 27.330452740996826 34.659503145063795 18.745365947778364 19.264678166161016 9 27.125075027512157 16.449710556663113 19.86141728524478 36.56379713057994 10-11 25.40422859285435 27.14705491839841 25.783381710642196 21.665334778105045 12-13 26.48979253867288 24.278144835771283 27.194061493175507 22.038001132380334 14-15 23.163398608304917 25.29161894825725 26.15962572522451 25.385356718213327 16-17 21.8765804262607 28.757852713845196 26.975591878181675 22.38997498171243 18-19 21.843237627981893 26.474803436620586 30.291223608590855 21.390735326806663 20-21 22.489479331003555 25.257959966134948 31.063255794293415 21.189304908568083 22-23 22.42741544067918 25.20692268521097 30.881184307890614 21.484477566219237 24-25 22.101078655686006 25.574796524392458 30.889305390692563 21.43481942922898 26-27 21.38375147881325 26.04561234430502 30.818841087809805 21.751795089071923 28-29 21.632045249525834 26.278859054728894 30.69286595912067 21.396229736624605 30-31 21.645294289355814 25.801532525514325 30.779704305820843 21.773468879309014 32-33 21.486138993470185 25.76137695562598 30.663563954769163 22.08892009613467 34-35 22.151005828649346 25.991767983852238 30.51636830539517 21.340857882103247 36-37 22.33943493070217 25.56091821585278 30.32109905831729 21.778547795127757 38-39 21.81260501986893 26.02053578221807 30.289831217312997 21.877027980600012 40-41 22.195708745270217 25.8403214040092 30.194532173470247 21.76943767725034 42-43 21.80801430884992 25.937103333293543 30.08452109520797 22.17036126264857 44-45 21.953932734571932 26.19615113213843 30.15623624702812 21.693679886261524 46-47 22.475631909430916 26.612426395959282 29.1004306964592 21.811510998150606 48-49 22.35561275410519 26.701986992081768 28.833588853708907 22.10881140010413 50-51 22.75080323571842 27.124627473172847 28.479896605001965 21.64467268610677 52-53 22.826097496753363 26.866246114667923 28.442085335207096 21.865571053371614 54-55 22.443631774170047 27.153395271538677 28.471591985594717 21.93138096869656 56-57 22.570364244585463 27.401017141828476 28.283246201133906 21.745372412452156 58-59 22.49736315901755 26.84624867412027 28.41699035619855 22.23939781066363 60-61 22.242605283428706 27.154911983466345 28.75365067588164 21.8488320572233 62-63 22.164482187088655 27.14086375003792 29.00967662209854 21.684977440774887 64-65 22.08735365594708 27.008586578641015 28.84189347311616 22.06216629229575 66-67 22.12790705191481 27.180820406886568 28.505183425173144 22.186089116025478 68-69 22.414590470374637 26.897443917711666 28.803229751025523 21.884735860888178 70-71 22.39855310654926 26.984020818238736 28.376760193920326 22.240665881291683 72-73 22.27196982091362 27.216649618161554 28.169592263078407 22.341788297846417 74-75 22.248075392020304 27.628797436408743 27.896832757397206 22.226294414173747 76-77 22.421825932193528 27.18489812420031 28.23344334882037 22.159832594785794 78-79 22.44967375775077 27.308721491410186 28.13393710071315 22.107667650125887 80-81 22.554998212269055 27.623128414777447 27.74046224406729 22.081411128886202 82-83 22.48735159821893 27.608624086614824 27.754129192222887 22.14989512294336 84-85 22.43668958315203 27.703071969367333 27.769832291109815 22.090406156370822 86-87 22.47120609429771 27.763436700152017 27.50721184089543 22.258145364654844 88-89 22.403923758621016 27.754062923156415 27.544135073888736 22.297878244333837 90-91 22.489898521450396 27.675353188073625 27.522522399309103 22.31222589116687 92-93 22.27307345826559 27.364833195872546 27.811827330022503 22.550266015839362 94-95 22.241386941060576 27.352084534063607 27.776788737941523 22.629739786934298 96-97 22.428191149463757 27.241265106823757 27.644088876335264 22.68645486737722 98-99 22.019324488293915 27.44629323891552 27.555521850695204 22.97886042209536 100-101 22.021800606561566 27.305093107896937 27.964248875255016 22.70885741028648 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 906.0 1 634.5 2 501.0 3 673.0 4 573.0 5 505.5 6 957.5 7 2108.0 8 3378.0 9 3972.5 10 3643.5 11 3220.5 12 3342.0 13 3607.0 14 3908.0 15 4060.0 16 4064.5 17 4232.0 18 4468.5 19 4686.5 20 5091.5 21 5724.0 22 6562.5 23 7856.0 24 9216.5 25 11020.0 26 13621.0 27 16351.0 28 18713.0 29 20947.0 30 23890.5 31 27922.0 32 33640.5 33 36480.5 34 37411.5 35 39817.5 36 44144.0 37 49086.5 38 51871.5 39 54044.0 40 55690.0 41 58770.0 42 60567.0 43 62693.0 44 66898.0 45 69507.0 46 70810.5 47 72959.0 48 72536.0 49 70767.5 50 71609.0 51 69552.5 52 66843.5 53 67701.5 54 70016.0 55 66231.0 56 59196.0 57 54305.0 58 50763.5 59 45155.0 60 35853.5 61 29696.0 62 25562.5 63 18929.0 64 13205.0 65 10109.0 66 7385.5 67 5657.0 68 4432.0 69 3162.0 70 2231.5 71 1494.5 72 1070.0 73 884.0 74 694.5 75 535.0 76 388.0 77 239.5 78 150.5 79 103.0 80 72.0 81 40.5 82 24.5 83 17.5 84 8.5 85 5.0 86 3.5 87 1.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040031249238536015 2 0.0 3 0.0 4 4.972825992364723E-5 5 0.008752173746561911 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005992255320799491 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.23233689503707E-4 22-23 0.0016410325774803584 24-25 0.0 26-27 0.001292934758014828 28-29 0.001740489097327653 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.729619494273542E-4 38-39 0.0 40-41 9.945651984729446E-5 42-43 0.021134510467550074 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.4864129961823615E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.4918477977094168E-4 84-85 1.9891303969458892E-4 86-87 0.0 88-89 0.0 90-91 0.010368342194080447 92-93 0.0026107336459914796 94-95 0.0 96-97 0.0 98-99 4.475543393128251E-4 100-101 0.012481793240835454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2010929.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.609012908153375 #Duplication Level Percentage of deduplicated Percentage of total 1 73.30316265211478 34.898912169129986 2 14.18446902725341 13.506171380276186 3 5.13313526174211 7.3315050880673205 4 2.300144708778236 4.380304765233749 5 1.2776526000737267 3.041388956452289 6 0.8031937395411627 2.294355666813791 7 0.5492776383525612 1.8305396322140959 8 0.3731623976073477 1.4212714723620543 9 0.2758421014634781 1.1819313152268192 >10 1.5899649073372168 14.17069705785143 >50 0.12892254514441 4.223897778629603 >100 0.07280966837676629 6.4933750563444 >500 0.00575254901028173 1.9264737438435664 >1k 0.0021964278039257514 1.7483067914171737 >5k 1.0459180018694054E-4 0.36255829530926914 >10k+ 2.0918360037388108E-4 1.188310830828238 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13052 0.6490532485234436 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10812 0.5376619462944738 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7281 0.36207146050407546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2784 0.1384434756274339 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2433 0.12098885639423372 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2418 0.12024293249537901 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2169 0.10786059577439083 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2140 0.10641847623660507 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2069 0.10288776978202611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.972825992364723E-5 6 0.0 0.0 0.0 0.0 1.9891303969458892E-4 7 0.0 0.0 0.0 0.0 1.9891303969458892E-4 8 0.0 0.0 0.0 0.0 1.9891303969458892E-4 9 0.0 0.0 0.0 0.0 1.9891303969458892E-4 10-11 0.0 0.0 0.0 0.0 1.9891303969458892E-4 12-13 0.0 0.0 0.0 0.0 2.7350542958005973E-4 14-15 0.0 0.0 0.0 0.0 3.23233689503707E-4 16-17 0.0 0.0 0.0 0.0 3.480978194655306E-4 18-19 0.0 0.0 0.0 0.0 3.480978194655306E-4 20-21 0.0 0.0 0.0 0.0 3.480978194655306E-4 22-23 0.0 0.0 0.0 4.972825992364723E-5 3.9782607938917784E-4 24-25 0.0 0.0 0.0 1.4918477977094168E-4 3.9782607938917784E-4 26-27 0.0 0.0 0.0 1.9891303969458892E-4 4.475543393128251E-4 28-29 0.0 0.0 0.0 9.448369385492973E-4 4.475543393128251E-4 30-31 0.0 0.0 0.0 0.0031328803751897753 4.475543393128251E-4 32-33 0.0 0.0 0.0 0.006290624880341374 4.724184692746487E-4 34-35 0.0 0.0 0.0 0.008553260706867325 5.470108591601196E-4 36-37 0.0 0.0 0.0 0.010268885674233154 5.470108591601196E-4 38-39 0.0 0.0 0.0 0.011338043262591569 5.718749891219432E-4 40-41 0.0 0.0 0.0 0.013724999738926635 5.967391190837667E-4 42-43 0.0 0.0 0.0 0.016286005124994465 5.967391190837667E-4 44-45 0.0 0.0 0.0 0.019344293110298772 6.46467379007414E-4 46-47 0.0 0.0 0.0 0.023297689774228726 6.961956389310612E-4 48-49 0.0 0.0 0.0 0.028295379896555273 7.210597688928848E-4 50-51 0.0 0.0 0.0 0.036550271043880714 7.956521587783557E-4 52-53 0.0 4.972825992364723E-5 0.0 0.05236385769960053 7.956521587783557E-4 54-55 0.0 4.972825992364723E-5 0.0 0.07568641160379108 7.956521587783557E-4 56-57 0.0 4.972825992364723E-5 0.0 0.10032676439595828 8.205162887401793E-4 58-59 0.0 9.945651984729446E-5 0.0 0.1365538017503353 8.453804187020029E-4 60-61 0.0 9.945651984729446E-5 0.0 0.18690366492302812 8.453804187020029E-4 62-63 0.0 9.945651984729446E-5 0.0 0.22367771313656526 8.951086786256502E-4 64-65 0.0 9.945651984729446E-5 0.0 0.26144632654857536 8.951086786256502E-4 66-67 0.0 9.945651984729446E-5 0.0 0.3284054285357663 8.951086786256502E-4 68-69 0.0 9.945651984729446E-5 0.0 0.4169465953298202 8.951086786256502E-4 70-71 0.0 9.945651984729446E-5 0.0 0.49462213733055715 8.951086786256502E-4 72-73 0.0 9.945651984729446E-5 0.0 0.5661562392307238 9.69701068511121E-4 74-75 0.0 9.945651984729446E-5 0.0 0.6820728131127454 0.0010691575883584156 76-77 0.0 9.945651984729446E-5 0.0 0.7781478112852318 0.0010940217183202392 78-79 0.0 9.945651984729446E-5 0.0 0.8625118042456994 0.0011188858482820627 80-81 0.0 9.945651984729446E-5 0.0 0.9685324544029152 0.0011437499782438862 82-83 0.0 9.945651984729446E-5 0.0 1.1467834020992287 0.0012183423681293572 84-85 0.0 9.945651984729446E-5 0.0 1.3221501107199707 0.001292934758014828 86-87 0.0 9.945651984729446E-5 0.0 1.5132806777365087 0.0013675271479002987 88-89 0.0 9.945651984729446E-5 0.0 1.7593112437087535 0.0013923912778621224 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5285 0.0 59.1608 1 GTATCAA 9795 0.0 46.280396 1 TGGTATC 1850 0.0 37.744904 2 TATCAAC 13285 0.0 34.111397 2 ATCAACG 13255 0.0 34.045254 3 TCAACGC 13470 0.0 33.572376 4 CAACGCA 13765 0.0 32.921898 5 AACGCAG 14195 0.0 32.02182 6 GTGGTAT 2355 0.0 30.064121 1 TAAGGTG 950 0.0 28.501251 5 CGCAGAG 16655 0.0 27.038116 8 ACGCAGA 16795 0.0 26.98243 7 GTAAGGT 965 0.0 26.08923 4 GTACATG 10575 0.0 25.252773 1 TACATGG 10445 0.0 25.19498 2 GCAGAGT 17920 0.0 24.784853 9 ACATGGG 10365 0.0 23.464611 3 GAGTACT 11690 0.0 22.836761 12-13 AGTACTT 11760 0.0 22.357487 12-13 GGACCGA 865 0.0 21.9641 6 >>END_MODULE