FastQCFastQC Report
Thu 26 May 2016
SRR937900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698711
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94780.5579524710206739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73690.4337995103346008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49680.29245704537146106No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24980.1470526770003844No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.10978912834496274No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18240.10737553356633353No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18050.10625703842501756No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17460.1027838166704048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.061.0528451
GTATCAA79450.045.910871
ATCAACG103400.034.7931943
TATCAAC104600.034.4869422
TCAACGC105750.034.1169474
CAACGCA108050.033.4787065
TGGTATC14600.033.204122
AACGCAG110400.032.829886
GTGGTAT17400.030.3380571
ACGCAGA129000.027.9522767
CGCAGAG128300.027.8826128
GTACATG92100.027.0573771
TACATGG91100.026.607032
ACATGGG92550.025.0075683
GCAGAGT139450.024.9037069
GAGTACT83300.024.4935212-13
TAAGGTG9050.024.1611735
GTACTTT87750.022.97800814-15
CAGAGTA132000.022.87078710-11
TAGGACG1050.002187403922.6354684
Produced by FastQC (version 0.11.5)